##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611913
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647936879915935	31.0	31.0	34.0	30.0	34.0
2	31.731347430108528	31.0	31.0	34.0	30.0	34.0
3	31.742654593054894	31.0	31.0	34.0	30.0	34.0
4	35.47717404271522	37.0	35.0	37.0	33.0	37.0
5	35.35445234861819	37.0	35.0	37.0	33.0	37.0
6	35.30779865765231	37.0	35.0	37.0	33.0	37.0
7	35.608236791831516	37.0	35.0	37.0	35.0	37.0
8	35.34912152544561	37.0	35.0	37.0	33.0	37.0
9	37.106075536881875	39.0	37.0	39.0	34.0	39.0
10	36.63863490398145	39.0	35.0	39.0	32.0	39.0
11	36.594605769120776	39.0	35.0	39.0	32.0	39.0
12	36.55286617542036	39.0	35.0	39.0	32.0	39.0
13	36.45235515506289	39.0	35.0	39.0	32.0	39.0
14	37.46171759710939	39.0	36.0	41.0	32.0	41.0
15	37.56839125823442	39.0	36.0	41.0	32.0	41.0
16	37.740317659536565	39.0	36.0	41.0	33.0	41.0
17	37.55686020725168	39.0	36.0	41.0	32.0	41.0
18	37.54450387555094	39.0	36.0	41.0	32.0	41.0
19	37.46105246987725	39.0	36.0	41.0	32.0	41.0
20	37.19190473155498	39.0	35.0	41.0	31.0	41.0
21	37.202726531386	39.0	35.0	41.0	32.0	41.0
22	37.19119548040326	39.0	35.0	41.0	32.0	41.0
23	36.969147574900354	39.0	35.0	41.0	31.0	41.0
24	36.995231348247216	39.0	35.0	41.0	31.0	41.0
25	36.99595530737213	39.0	35.0	41.0	31.0	41.0
26	36.931134001075314	39.0	35.0	41.0	31.0	41.0
27	36.79769673139809	39.0	35.0	41.0	31.0	41.0
28	36.685231397273796	39.0	35.0	41.0	30.0	41.0
29	36.63944711094551	39.0	35.0	41.0	30.0	41.0
30	36.55189708340892	39.0	35.0	40.0	30.0	41.0
31	36.48981309434511	39.0	35.0	40.0	30.0	41.0
32	36.16294309812016	38.0	35.0	40.0	30.0	41.0
33	36.04033416515093	38.0	35.0	40.0	29.0	41.0
34	35.87976068493397	38.0	35.0	40.0	29.0	41.0
35	35.71165345400408	38.0	35.0	40.0	27.0	41.0
36	35.60063113547187	38.0	35.0	40.0	27.0	41.0
37	35.490365460449446	38.0	35.0	40.0	27.0	41.0
38	35.37825311768176	38.0	35.0	40.0	26.0	41.0
39	35.19418119896129	38.0	34.0	40.0	26.0	41.0
40	35.127601472758386	38.0	34.0	40.0	25.0	41.0
41	35.011214012449486	38.0	34.0	40.0	24.0	41.0
42	34.95860032390225	38.0	34.0	40.0	24.0	41.0
43	34.963937683951805	38.0	34.0	40.0	25.0	41.0
44	35.01043448987029	38.0	34.0	40.0	25.0	41.0
45	34.93774768635411	38.0	34.0	40.0	25.0	41.0
46	34.730079275975505	37.0	34.0	40.0	24.0	41.0
47	34.55139374388189	37.0	34.0	40.0	24.0	41.0
48	34.554485686690754	37.0	34.0	40.0	24.0	41.0
49	34.46002618019228	37.0	33.0	40.0	24.0	41.0
50	34.32370124511164	37.0	33.0	40.0	23.0	41.0
51	32.8586334985529	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	7.0
12	4.0
13	8.0
14	19.0
15	32.0
16	69.0
17	153.0
18	305.0
19	637.0
20	1340.0
21	2619.0
22	4190.0
23	5157.0
24	5742.0
25	6482.0
26	7098.0
27	7875.0
28	8932.0
29	10660.0
30	13611.0
31	17104.0
32	22026.0
33	29020.0
34	44167.0
35	54163.0
36	55205.0
37	75033.0
38	113356.0
39	126870.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.16483225556574	17.096548038691775	20.416791275883988	13.321828429858492
2	28.122461853237308	32.712983708468364	23.322106247129902	15.84244819116443
3	29.188299643903626	31.872995017265527	24.683247455112085	14.255457883718764
4	25.425673257472877	24.02106181761133	32.8440480918039	17.709216833111896
5	29.192385192012587	27.06430489301584	23.90519567324113	19.83811424173044
6	20.678920042555067	41.14343052035175	24.72344924850428	13.4542001885889
7	72.38725112883694	6.48507222431947	16.397919312059066	4.72975733478452
8	72.1143365151582	13.245837235031777	9.381235567801305	5.258590682008717
9	68.38635557669146	7.95178399543726	10.182983528704243	13.47887689916704
10	39.96401449225625	24.04263351162665	18.15944423471964	17.83390776139745
11	31.036274764549866	24.939166188657538	25.030682466298316	18.993876580494288
12	26.115640622114583	21.36594581255832	29.286189376594386	23.23222418873271
13	25.473555881309924	22.773498847058324	33.13167067867491	18.62127459295684
14	19.521729396172333	30.94361453343858	26.708045097914244	22.826610972474846
15	17.533701686350838	25.43515172908567	35.28410084440108	21.747045740162406
16	18.960865351773865	24.23498111659664	29.497657346714323	27.306496184915176
17	19.10075451902476	26.139990488844	30.115392220789555	24.643862771341677
18	20.452907521167223	25.20080468955554	29.565967711096185	24.78032007818105
19	21.176539802226788	27.127549177742587	26.149632382381157	25.546278637649472
20	24.970543198134376	24.488448521276716	29.542108110139843	20.998900170449065
21	21.864219259927474	30.690800816455933	27.570912858527276	19.874067065089317
22	20.225424202460154	24.053092514785597	28.087979827197657	27.63350345555659
23	20.93974143383128	29.173428248787005	27.331009473568958	22.555820843812764
24	20.609629146627054	26.599369518215827	27.752964882262678	25.038036452894445
25	19.35454876755356	31.99360039744212	25.8763909248537	22.775459910150627
26	18.811987978683245	25.39086438758451	29.962429299589978	25.83471833414227
27	23.21114276049046	26.584824966947917	25.531080398684125	24.672951873877498
28	19.132785216199036	26.907746689480366	30.51430513814872	23.445162956171874
29	22.75160030919428	23.619697571386784	27.729268703230687	25.89943341618825
30	21.082081929947556	28.98010011227086	25.95254554160477	23.98527241617681
31	23.638981358461088	25.05143705069185	23.71546281906088	27.594118771786185
32	25.74058730571176	26.009906637054613	25.13053326208137	23.118972795152253
33	24.492043803612603	23.77543866530046	24.57489871926238	27.15761881182456
34	22.00443527102709	23.969747333362747	27.28672213206779	26.739095263542367
35	22.424756460477223	24.53584087934069	25.475843788250945	27.563558871931143
36	25.033460639012407	25.070393993917435	26.15355450856576	23.742590858504396
37	20.993507246945235	24.843727784832158	31.016173867853762	23.146591100368845
38	25.241823592569535	25.40181365651653	25.799909464253908	23.55645328666003
39	22.901621635755408	23.474088636783332	31.02336443252554	22.600925294935717
40	22.867956719337553	26.010560324752046	28.325595305214957	22.79588765069544
41	19.429069165061048	25.054868911103377	30.38454813020805	25.131513793627526
42	20.626134760987263	25.490551761443214	29.75177843909183	24.131535038477693
43	22.53424914979744	22.84736555686838	28.114944444716816	26.50344084861737
44	23.7953761400722	22.71711828315463	27.93109477981347	25.5564107969597
45	22.0142405864886	22.554840312266613	28.740686993085617	26.69023210815917
46	24.96792844734464	26.745958984365426	26.25373214819754	22.032380420092398
47	20.577761871377138	24.087737962749607	32.38156404586927	22.95293612000399
48	22.910773263519488	24.80695785185149	27.13294210124642	25.1493267833826
49	20.538540609531093	22.845077649927358	31.979872955796	24.636508784745544
50	22.4185464273516	21.649809695169083	30.01292667421676	25.918717203262553
51	21.73233776697014	22.86746645356448	27.250442464860203	28.14975331460518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2802.0
1	2860.0
2	2918.0
3	3354.5
4	3791.0
5	2694.0
6	1597.0
7	1696.5
8	1796.0
9	1787.5
10	1779.0
11	1761.5
12	1744.0
13	1806.5
14	1869.0
15	1877.0
16	1885.0
17	1921.0
18	1957.0
19	1785.0
20	1613.0
21	1883.5
22	2154.0
23	2137.5
24	2121.0
25	2841.5
26	4170.0
27	4778.0
28	5405.0
29	6032.0
30	7149.0
31	8266.0
32	9196.0
33	10126.0
34	11777.5
35	13429.0
36	14260.5
37	15092.0
38	15828.5
39	16565.0
40	19217.5
41	21870.0
42	24257.0
43	26644.0
44	31268.5
45	35893.0
46	59542.5
47	83192.0
48	69259.0
49	55326.0
50	56182.0
51	57038.0
52	49976.5
53	42915.0
54	39326.0
55	35737.0
56	32845.5
57	29954.0
58	27713.0
59	25472.0
60	23601.5
61	21731.0
62	19713.5
63	17696.0
64	16100.0
65	14504.0
66	11850.0
67	9196.0
68	8416.0
69	7636.0
70	7284.0
71	6932.0
72	6101.0
73	5270.0
74	4478.0
75	3028.0
76	2370.0
77	2005.5
78	1641.0
79	1171.0
80	701.0
81	500.5
82	300.0
83	230.0
84	160.0
85	117.5
86	75.0
87	60.5
88	46.0
89	36.5
90	27.0
91	18.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	3.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	611913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.893248262990166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62137314633156	20.068111137495222
2	10.375594951468942	5.580669018121634
3	3.762548727901491	3.0356147112315788
4	1.8592897321269664	2.00009361435676
5	1.1330037162182016	1.523507511157328
6	0.7927380140676588	1.2791580131898803
7	0.5551894799085697	1.045159396132709
8	0.46397560913645747	0.9982248995583072
9	0.3696459064154291	0.8946881217565348
>10	4.934183560301202	32.20483679908557
>50	1.0206170808020685	18.090505287909142
>100	0.0996171162699025	4.493699395616563
>500	0.0030557397628804446	0.5226569649266914
>1k	0.007333775430913066	3.493255073129528
>5k	0.001222295905152178	2.3831842740368003
>10k+	6.11147952576089E-4	2.3866357822957505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	14521	2.3730497636101866	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	8203	1.340550045513006	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	6297	1.0290678576856513	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	2712	0.44320025886032816	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	2617	0.42767517604626804	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2228	0.3641040474708006	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT	2226	0.36377720362208354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1881	0.3073966397183913	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	1749	0.2858249457030656	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	1545	0.2524868731339259	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA	1534	0.2506892319659821	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1283	0.20967032895199156	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1237	0.20215292043149927	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1165	0.19038654187768522	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	1077	0.17600541253413476	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	892	0.14577235652780707	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	677	0.11063664279072352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6342192435852808E-4	0.0	0.0	0.18515704029821234	0.0
2	1.6342192435852808E-4	0.0	0.0	1.0418147677856167	0.0
3	1.6342192435852808E-4	0.0	0.0	1.3078656606413004	0.0
4	1.6342192435852808E-4	0.0	0.0	1.913343890389647	0.0
5	1.6342192435852808E-4	0.0	0.0	4.263678006513998	0.0
6	1.6342192435852808E-4	0.0	0.0	5.026858393268324	0.0
7	1.6342192435852808E-4	0.0	0.0	6.0837079780949255	0.0
8	1.6342192435852808E-4	0.0	0.0	7.2711316804839905	0.0
9	1.6342192435852808E-4	0.0	0.0	7.6517413423150025	0.0
10	1.6342192435852808E-4	0.0	0.0	11.028038299562192	0.0
11	1.6342192435852808E-4	0.0	0.0	12.228372333975582	0.0
12	1.6342192435852808E-4	0.0	0.0	14.338312799368538	0.0
13	1.6342192435852808E-4	0.0	0.0	14.81877325698261	0.0
14	1.6342192435852808E-4	0.0	0.0	15.060474283108872	0.0
15	1.6342192435852808E-4	0.0	0.0	15.572475172124141	0.0
16	1.6342192435852808E-4	0.0	0.0	16.250185892438957	0.0
17	1.6342192435852808E-4	0.0	0.0	16.97937451892671	0.0
18	1.6342192435852808E-4	0.0	0.0	17.901891281930602	0.0
19	1.6342192435852808E-4	0.0	0.0	18.721779076437336	0.0
20	1.6342192435852808E-4	0.0	0.0	19.21678408531932	0.0
21	1.6342192435852808E-4	0.0	0.0	19.73728291440123	0.0
22	1.6342192435852808E-4	0.0	0.0	20.349624864972636	0.0
23	4.902657730755843E-4	0.0	0.0	20.89529067040576	0.0
24	4.902657730755843E-4	0.0	0.0	21.329012457653295	0.0
25	4.902657730755843E-4	0.0	0.0	21.72122507611376	0.0
26	4.902657730755843E-4	0.0	0.0	22.06522822688846	0.0
27	4.902657730755843E-4	0.0	0.0	22.468880380054028	0.0
28	8.171096217926405E-4	0.0	0.0	22.847528978792738	0.0
29	8.171096217926405E-4	0.0	0.0	23.22487020213658	0.0
30	8.171096217926405E-4	0.0	0.0	23.65205511240977	0.0
31	8.171096217926405E-4	0.0	0.0	24.027598694585667	0.0
32	8.171096217926405E-4	0.0	0.0	24.412457326450003	0.0
33	8.171096217926405E-4	0.0	0.0	24.808755493019433	0.0
34	8.171096217926405E-4	0.0	0.0	25.172696118565874	0.0
35	8.171096217926405E-4	0.0	0.0	25.572426145546835	0.0
36	8.171096217926405E-4	0.0	0.0	25.9406157411266	0.0
37	8.171096217926405E-4	0.0	0.0	26.31779354254608	0.0
38	8.171096217926405E-4	0.0	0.0	26.684675762730976	0.0
39	8.171096217926405E-4	0.0	0.0	27.052211670613307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	60	0.0	45.000004	1
CGAATTC	30	2.1638316E-6	45.000004	44
CCGAACT	30	2.1638316E-6	45.000004	11
TCGCGAC	30	2.1638316E-6	45.000004	1
CTTACCG	30	2.1638316E-6	45.000004	25
CGTATTA	20	7.0306985E-4	45.0	17
TTTACGT	20	7.0306985E-4	45.0	36
ACGTTTA	20	7.0306985E-4	45.0	39
CGCTCGA	20	7.0306985E-4	45.0	42
AACGCTC	20	7.0306985E-4	45.0	22
CTCGAAT	35	1.210683E-7	45.0	43
AACTCGT	20	7.0306985E-4	45.0	39
CGCCGTG	20	7.0306985E-4	45.0	45
GCGTCTA	20	7.0306985E-4	45.0	34
CGTTTAG	20	7.0306985E-4	45.0	40
CGTCAAT	40	6.8066583E-9	45.0	14
TGAGACG	20	7.0306985E-4	45.0	13
AACACGC	35	1.210683E-7	45.0	28
AACGTCG	25	3.888661E-5	44.999996	17
ACTTAGA	25	3.888661E-5	44.999996	20
>>END_MODULE