FastQCFastQC Report
Sat 18 Jun 2016
SRR3553627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500540
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24220.4838774123946138No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15960.3188556359132137No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC13590.27150677268549966No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13380.26731130379190476No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC11020.220162224797219TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC10150.20278099652375434No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC9720.19419027450353618TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT9080.1814040835897231TruSeq Adapter, Index 13 (95% over 23bp)
TGGAGCGGGTTTGGGCCAATATTTTTCCTCTTACTATTTCCTTTTCTTCTT8380.16741918727774005No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG7860.15703040716026692No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6480.12946018300235745No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5490.10968154393255283No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC5470.10928197546649619No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5390.10768370160226955No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC5350.10688456467015622TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG750.045.0000042
CGAACCT207.0294656E-445.038
TTTACGG351.2100827E-745.02
ACGCATT1700.045.017
TATTGCG207.0294656E-445.01
CCGTACT502.1827873E-1145.044
TACGCAT1700.045.016
TGACCGT207.0294656E-445.031
GTTCGTC207.0294656E-445.037
TACGAAT850.045.012
AACGATC207.0294656E-445.041
CGAATAT850.045.014
GCGATAT207.0294656E-445.09
CGGTCTA207.0294656E-445.031
TATAACG253.8876384E-545.01
AGTACGG453.8380676E-1045.02
TACACGA207.0294656E-445.022
TACCGTT207.0294656E-445.038
GTGAGTC302.1630367E-644.99999617
TGGGTAC950.042.631586
Produced by FastQC (version 0.11.5)