##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500540
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.785663483437887	31.0	31.0	34.0	30.0	34.0
2	31.889757062372638	33.0	31.0	34.0	30.0	34.0
3	31.880918607903464	33.0	31.0	34.0	30.0	34.0
4	35.55384984217046	37.0	35.0	37.0	33.0	37.0
5	35.47262956007512	37.0	35.0	37.0	33.0	37.0
6	35.45438925959963	37.0	35.0	37.0	33.0	37.0
7	35.79372277939825	37.0	35.0	37.0	35.0	37.0
8	35.71955288288648	37.0	35.0	37.0	35.0	37.0
9	37.41040476285612	39.0	37.0	39.0	35.0	39.0
10	36.8396951292604	39.0	37.0	39.0	32.0	39.0
11	36.818919566867784	39.0	37.0	39.0	32.0	39.0
12	36.74221840412355	39.0	35.0	39.0	33.0	39.0
13	36.657246174131934	39.0	35.0	39.0	32.0	39.0
14	37.72399208854437	40.0	36.0	41.0	33.0	41.0
15	37.86349342709873	40.0	36.0	41.0	33.0	41.0
16	37.90341431254245	40.0	36.0	41.0	33.0	41.0
17	37.8439505334239	40.0	36.0	41.0	33.0	41.0
18	37.79132736644424	39.0	36.0	41.0	33.0	41.0
19	37.71091421265034	39.0	36.0	41.0	33.0	41.0
20	37.61981859591641	39.0	35.0	41.0	33.0	41.0
21	37.47799576457426	39.0	35.0	41.0	33.0	41.0
22	37.48077476325568	39.0	35.0	41.0	33.0	41.0
23	37.39868142406201	39.0	35.0	41.0	32.0	41.0
24	37.39020058336996	39.0	35.0	41.0	33.0	41.0
25	37.26216086626444	39.0	35.0	41.0	32.0	41.0
26	37.23854836776282	39.0	35.0	41.0	32.0	41.0
27	37.206115395373	39.0	35.0	41.0	32.0	41.0
28	37.11825028968714	39.0	35.0	41.0	32.0	41.0
29	37.0799316737923	39.0	35.0	41.0	32.0	41.0
30	36.906672793383144	39.0	35.0	41.0	31.0	41.0
31	36.817682902465336	39.0	35.0	41.0	31.0	41.0
32	36.673856235265916	39.0	35.0	41.0	31.0	41.0
33	36.54673352778999	39.0	35.0	41.0	31.0	41.0
34	36.450239741079635	39.0	35.0	41.0	30.0	41.0
35	36.38159987213809	39.0	35.0	41.0	30.0	41.0
36	36.222273944140326	39.0	35.0	40.0	30.0	41.0
37	36.14760458704599	38.0	35.0	40.0	30.0	41.0
38	36.04193870619731	38.0	35.0	40.0	30.0	41.0
39	35.97527670116274	38.0	35.0	40.0	29.0	41.0
40	35.82331082430974	38.0	35.0	40.0	29.0	41.0
41	35.706233268070484	38.0	35.0	40.0	28.0	41.0
42	35.603090662084945	38.0	35.0	40.0	28.0	41.0
43	35.51013505414153	38.0	35.0	40.0	28.0	41.0
44	35.399878131617854	38.0	34.0	40.0	27.0	41.0
45	35.35406161345747	38.0	34.0	40.0	27.0	41.0
46	35.17076956886562	38.0	34.0	40.0	26.0	41.0
47	35.115473288848044	37.0	34.0	40.0	26.0	41.0
48	35.052199624405645	37.0	34.0	40.0	26.0	41.0
49	34.96880968553962	37.0	34.0	40.0	26.0	41.0
50	34.837547448755345	37.0	34.0	40.0	26.0	41.0
51	33.32359651576298	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	6.0
12	4.0
13	12.0
14	21.0
15	50.0
16	58.0
17	119.0
18	213.0
19	397.0
20	666.0
21	1111.0
22	1606.0
23	2271.0
24	2904.0
25	3941.0
26	5045.0
27	5938.0
28	7008.0
29	8285.0
30	10152.0
31	12665.0
32	16650.0
33	22188.0
34	36618.0
35	46180.0
36	43209.0
37	60628.0
38	96289.0
39	116265.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11599472569625	20.312862108922364	24.172893275262716	14.398249890118672
2	31.38490430335238	25.17481120389979	26.1417668917569	17.29851760099093
3	31.757501897950213	25.14624205857674	26.61046070244136	16.485795341031686
4	27.461341750909018	26.199704319335115	26.763095856475005	19.57585807328086
5	23.9725096895353	30.760378790905822	25.025772166060655	20.241339353498223
6	22.28393335197986	36.36732329084589	25.963359571662604	15.385383785511648
7	78.25927997762416	7.153873816278419	9.572261957086345	5.014584249011068
8	79.2623966116594	6.495185199984017	8.908778519199265	5.33363966915731
9	74.00527430375195	8.74075997922244	10.962160866264433	6.291804850761178
10	39.75166819834578	24.975426539337516	18.825868062492507	16.44703719982419
11	29.82399009070204	26.63783114236624	24.342709873336794	19.195468893594917
12	26.521356934510727	22.65952771007312	31.07503895792544	19.74407639749071
13	23.582331082430976	25.612738242697887	31.36452631158349	19.44040436328765
14	20.413353578135613	28.04591041674991	30.188396531745713	21.35233947336876
15	19.63998881208295	25.758580732808568	34.042034602629165	20.559395852479323
16	20.505254325328647	24.368482039397453	33.07647740440324	22.04978623087066
17	20.516042673912175	24.92248371758501	29.466975666280415	25.0944979422224
18	22.119910496663604	25.152635154033643	30.15543213329604	22.57202221600671
19	23.937547448755346	27.063371558716586	27.12490510248931	21.874175890038757
20	23.94294162304711	25.917608982299118	28.340991728932753	21.79845766572102
21	22.517481120389977	28.153793902585207	28.60890238542374	20.71982259160107
22	21.982059375874055	24.858552763015943	27.77240580173413	25.386982059375875
23	20.629719902505293	27.881487992967596	27.727054780836696	23.761737323690415
24	22.03799896112199	24.597834338913973	30.187197826347546	23.176968873616495
25	20.416749910097096	27.325088903983698	28.36057058376953	23.89759060214968
26	19.711111999041037	27.790785951172737	29.11615455308267	23.38194749670356
27	22.144683741559117	26.789067806768692	28.739361489591243	22.326886962080952
28	19.283973308826468	27.037000039956848	30.782954409238023	22.89607224197866
29	21.382307108323012	25.97854317337276	29.01386502577217	23.625284692532063
30	22.577016821832423	26.37311703360371	28.258281056459023	22.791585088104846
31	23.22911255843689	25.927398409717505	27.253166580093495	23.590322451752108
32	22.035002197626564	27.169656770687656	27.479921684580656	23.315419347105127
33	23.35817317297319	26.574499540496266	25.467894673752344	24.599432612778198
34	20.405961561513568	25.23894194270188	28.956726735126065	25.39836976065849
35	22.275142845726613	23.740959763455468	28.804491149558476	25.17940624125944
36	24.265193590921804	27.03080672873297	27.311303791904745	21.392695888440485
37	21.448635473688416	26.7940624125944	28.84564670156231	22.91165541215487
38	21.714748072082152	27.903064690134656	27.087545450913016	23.29464178687018
39	22.831142366244457	25.38977903863827	26.583689615215565	25.195388979901708
40	22.681703759939264	24.583250089902904	29.572261957086347	23.162784193071484
41	20.443920565788947	24.57306109401846	28.980700843089462	26.002317497103128
42	22.591800855076517	23.98569544891517	29.5035361809246	23.918967515083708
43	22.777600191792864	23.51800055939585	29.943461062052982	23.760938186758302
44	21.56051464418428	24.771247053182563	28.61729332321093	25.050944979422223
45	22.242977584209054	24.313141806848606	27.552643145402968	25.89123746353938
46	22.45654692931634	25.868661845207175	27.878890797938226	23.795900427538257
47	20.70783553761937	24.719902505294282	30.46709553682023	24.105166420266112
48	20.726215687057977	24.527110720421945	30.14204658968314	24.60462700283694
49	22.280137451552324	23.436488592320295	29.935869261197904	24.347504694929476
50	20.88284652575219	23.445878451272627	29.846565709034245	25.824709313940943
51	21.20549806209294	22.944619810604546	27.591800855076514	28.258081272225994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3020.0
1	3036.0
2	3052.0
3	3246.0
4	3440.0
5	2494.5
6	1549.0
7	1520.0
8	1491.0
9	1565.5
10	1640.0
11	1687.5
12	1735.0
13	1676.0
14	1617.0
15	1787.5
16	1958.0
17	1747.0
18	1536.0
19	1566.0
20	1596.0
21	1708.5
22	1821.0
23	2113.5
24	2406.0
25	2544.0
26	3517.5
27	4353.0
28	5772.5
29	7192.0
30	7777.5
31	8363.0
32	8793.5
33	9224.0
34	10491.5
35	11759.0
36	12318.5
37	12878.0
38	13873.0
39	14868.0
40	16852.0
41	18836.0
42	22849.5
43	26863.0
44	29433.0
45	32003.0
46	37787.5
47	43572.0
48	46404.5
49	49237.0
50	47555.0
51	45873.0
52	41369.0
53	36865.0
54	31776.5
55	26688.0
56	24814.0
57	22940.0
58	21645.0
59	20350.0
60	19570.0
61	18790.0
62	16860.5
63	14931.0
64	13462.0
65	11993.0
66	10508.5
67	9024.0
68	7896.0
69	6768.0
70	6176.5
71	5585.0
72	5044.0
73	4503.0
74	3787.5
75	2426.0
76	1780.0
77	1478.5
78	1177.0
79	994.5
80	812.0
81	558.0
82	304.0
83	270.0
84	236.0
85	160.0
86	84.0
87	58.0
88	32.0
89	26.5
90	21.0
91	18.0
92	15.0
93	9.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	500540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.5376371077255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.84290854619286	21.81145035616407
2	10.591355074674686	6.2568720534961555
3	4.03042761010723	3.5714792440891414
4	2.045400475952241	2.416651879945853
5	1.1671171848807387	1.7236941934598717
6	0.7974399493638272	1.4132695103707036
7	0.6477853783770102	1.3393834601133547
8	0.46782199546992737	1.1054685066562173
9	0.38413505817508603	1.0211797753857645
>10	5.108309149358071	36.37705422598254
>50	0.8263837909260786	15.827648032666522
>100	0.08073864623990423	4.098899506345164
>500	0.0061062841694045225	1.2669653683623003
>1k	0.004070856112936348	1.7699838869623274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2422	0.4838774123946138	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1596	0.3188556359132137	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1359	0.27150677268549966	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1338	0.26731130379190476	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	1102	0.220162224797219	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	1015	0.20278099652375434	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	972	0.19419027450353618	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	908	0.1814040835897231	TruSeq Adapter, Index 13 (95% over 23bp)
TGGAGCGGGTTTGGGCCAATATTTTTCCTCTTACTATTTCCTTTTCTTCTT	838	0.16741918727774005	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	786	0.15703040716026692	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	648	0.12946018300235745	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	549	0.10968154393255283	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	547	0.10928197546649619	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	539	0.10768370160226955	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	535	0.10688456467015622	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.997842330283294E-4	0.0	0.0	0.18739761058057297	0.0
2	1.997842330283294E-4	0.0	0.0	0.8217125504455188	0.0
3	1.997842330283294E-4	0.0	0.0	1.1127981779677947	0.0
4	1.997842330283294E-4	0.0	0.0	1.5503256482998362	0.0
5	1.997842330283294E-4	0.0	0.0	2.8015742997562634	0.0
6	1.997842330283294E-4	0.0	0.0	3.681424062013026	0.0
7	1.997842330283294E-4	0.0	0.0	4.481360131058457	0.0
8	1.997842330283294E-4	0.0	0.0	5.689455388180765	0.0
9	1.997842330283294E-4	0.0	0.0	6.198505613936948	0.0
10	1.997842330283294E-4	0.0	0.0	7.4889119750669275	0.0
11	1.997842330283294E-4	0.0	0.0	9.06361129979622	0.0
12	1.997842330283294E-4	0.0	0.0	10.299476565309465	0.0
13	1.997842330283294E-4	0.0	0.0	10.750189795021377	0.0
14	1.997842330283294E-4	0.0	0.0	10.934390857873497	0.0
15	1.997842330283294E-4	0.0	0.0	11.258241099612418	0.0
16	1.997842330283294E-4	0.0	0.0	11.972469732688696	0.0
17	1.997842330283294E-4	0.0	0.0	12.897071163143805	0.0
18	1.997842330283294E-4	0.0	0.0	13.998681424062013	0.0
19	1.997842330283294E-4	0.0	0.0	14.553881807647741	0.0
20	1.997842330283294E-4	0.0	0.0	15.027770008390938	0.0
21	1.997842330283294E-4	0.0	0.0	15.655092500099892	0.0
22	1.997842330283294E-4	0.0	0.0	16.27981779677948	0.0
23	1.997842330283294E-4	0.0	0.0	16.941702960802335	0.0
24	1.997842330283294E-4	0.0	0.0	17.43337195828505	0.0
25	1.997842330283294E-4	0.0	0.0	17.886083030327246	0.0
26	1.997842330283294E-4	0.0	0.0	18.29584049226835	0.0
27	1.997842330283294E-4	0.0	0.0	18.733767531066448	0.0
28	1.997842330283294E-4	0.0	0.0	19.175290686059057	0.0
29	1.997842330283294E-4	0.0	0.0	19.638390538218722	0.0
30	1.997842330283294E-4	0.0	0.0	20.138450473488632	0.0
31	1.997842330283294E-4	0.0	0.0	20.59895313061893	0.0
32	1.997842330283294E-4	0.0	0.0	21.05366204499141	0.0
33	1.997842330283294E-4	0.0	0.0	21.515563191752907	0.0
34	1.997842330283294E-4	0.0	0.0	21.958484836376712	0.0
35	1.997842330283294E-4	0.0	0.0	22.45534822391817	0.0
36	1.997842330283294E-4	0.0	0.0	22.885283893395133	0.0
37	1.997842330283294E-4	0.0	0.0	23.32361050065929	0.0
38	1.997842330283294E-4	0.0	0.0	23.749550485475687	0.0
39	3.995684660566588E-4	0.0	0.0	24.204259399848166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	75	0.0	45.000004	2
CGAACCT	20	7.0294656E-4	45.0	38
TTTACGG	35	1.2100827E-7	45.0	2
ACGCATT	170	0.0	45.0	17
TATTGCG	20	7.0294656E-4	45.0	1
CCGTACT	50	2.1827873E-11	45.0	44
TACGCAT	170	0.0	45.0	16
TGACCGT	20	7.0294656E-4	45.0	31
GTTCGTC	20	7.0294656E-4	45.0	37
TACGAAT	85	0.0	45.0	12
AACGATC	20	7.0294656E-4	45.0	41
CGAATAT	85	0.0	45.0	14
GCGATAT	20	7.0294656E-4	45.0	9
CGGTCTA	20	7.0294656E-4	45.0	31
TATAACG	25	3.8876384E-5	45.0	1
AGTACGG	45	3.8380676E-10	45.0	2
TACACGA	20	7.0294656E-4	45.0	22
TACCGTT	20	7.0294656E-4	45.0	38
GTGAGTC	30	2.1630367E-6	44.999996	17
TGGGTAC	95	0.0	42.63158	6
>>END_MODULE