SRR3553624_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298632
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	2739	0.9171823515229447	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2119	0.7095689678266227	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	1952	0.6536472983471295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	1562	0.5230517827962174	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1300	0.4353183851697072	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.39680945109700233	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1153	0.38609392161590184	No Hit
AAGTGAGGGGTTTTTTCTGCAAGTACTTATCCTTGCTTTTTCTGAATACAT	939	0.3144338182110423	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	925	0.3097457740630609	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	594	0.19890701599292776	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	503	0.16843472903104825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	473	0.15838892014251654	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.14599908917999413	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA	377	0.1262423316992151	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	368	0.12322858903265559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTC	365	0.12222400814380241	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	365	0.12222400814380241	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	336	0.11251305955155509	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	314	0.1051461330332985	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGC	115	0.0	45.000004	45
TATCATG	55	1.8189894E-12	45.0	28
CGTGGTT	20	7.024891E-4	45.0	23
CTAGACT	20	7.024891E-4	45.0	38
CGGGTAG	20	7.024891E-4	45.0	6
TCGTTGC	20	7.024891E-4	45.0	16
CACGGGC	55	1.8189894E-12	45.0	4
TGGACTA	20	7.024891E-4	45.0	23
TAGGTCG	20	7.024891E-4	45.0	1
CCTACTA	20	7.024891E-4	45.0	35
TTACTAG	20	7.024891E-4	45.0	30
ATATGAC	20	7.024891E-4	45.0	36
TAACGCG	20	7.024891E-4	45.0	1
CGACAAA	20	7.024891E-4	45.0	12
CATTCGT	20	7.024891E-4	45.0	20
ACTTACC	20	7.024891E-4	45.0	23
ATCACGC	50	2.1827873E-11	45.0	42
CATTATC	20	7.024891E-4	45.0	42
GCACCGC	20	7.024891E-4	45.0	26
ATCGTGA	20	7.024891E-4	45.0	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content