##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298632
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.819888692437516	31.0	31.0	34.0	30.0	34.0
2	31.9167537303437	33.0	31.0	34.0	30.0	34.0
3	31.921354710814647	33.0	31.0	34.0	30.0	34.0
4	35.60324747515337	37.0	35.0	37.0	33.0	37.0
5	35.515815451793515	37.0	35.0	37.0	33.0	37.0
6	35.489267727504085	37.0	35.0	37.0	33.0	37.0
7	35.741715556269924	37.0	35.0	37.0	35.0	37.0
8	35.578802673524606	37.0	35.0	37.0	33.0	37.0
9	37.320819604061185	39.0	37.0	39.0	34.0	39.0
10	36.87857965656728	39.0	37.0	39.0	32.0	39.0
11	36.81393822497254	39.0	37.0	39.0	33.0	39.0
12	36.674907578558226	39.0	35.0	39.0	33.0	39.0
13	36.49925326153929	39.0	35.0	39.0	32.0	39.0
14	37.53402515470545	40.0	36.0	41.0	32.0	41.0
15	37.693197647941275	40.0	36.0	41.0	33.0	41.0
16	37.8227383535589	40.0	36.0	41.0	33.0	41.0
17	37.685033753917864	39.0	36.0	41.0	33.0	41.0
18	37.675791609740415	39.0	36.0	41.0	33.0	41.0
19	37.50033151169332	39.0	36.0	41.0	33.0	41.0
20	37.309574995311955	39.0	35.0	41.0	32.0	41.0
21	37.23420798842723	39.0	35.0	41.0	32.0	41.0
22	37.242854081277294	39.0	35.0	41.0	32.0	41.0
23	37.04540705617617	39.0	35.0	41.0	32.0	41.0
24	37.05975581987195	39.0	35.0	41.0	32.0	41.0
25	36.9986572102119	39.0	35.0	41.0	32.0	41.0
26	36.907501540357366	39.0	35.0	41.0	32.0	41.0
27	36.78208631358997	39.0	35.0	41.0	31.0	41.0
28	36.73324693937689	39.0	35.0	41.0	31.0	41.0
29	36.685894344879316	39.0	35.0	41.0	31.0	41.0
30	36.56132631466152	38.0	35.0	40.0	31.0	41.0
31	36.48055466259476	38.0	35.0	40.0	31.0	41.0
32	36.19008009858287	38.0	35.0	40.0	30.0	41.0
33	35.98459977497388	38.0	35.0	40.0	30.0	41.0
34	35.758860403439684	38.0	35.0	40.0	29.0	41.0
35	35.6629597631868	38.0	35.0	40.0	28.0	41.0
36	35.50353947333173	38.0	35.0	40.0	27.0	41.0
37	35.36552680221811	38.0	35.0	40.0	27.0	41.0
38	35.34387473546037	38.0	34.0	40.0	26.0	41.0
39	35.211005518497686	38.0	34.0	40.0	26.0	41.0
40	35.039677596506735	38.0	34.0	40.0	25.0	41.0
41	34.85498874869405	37.0	34.0	40.0	24.0	41.0
42	34.698408074151466	37.0	34.0	40.0	24.0	41.0
43	34.60945578504648	37.0	34.0	40.0	24.0	41.0
44	34.573475046210724	37.0	34.0	40.0	23.0	41.0
45	34.55459897130917	37.0	34.0	40.0	23.0	41.0
46	34.389167939135795	37.0	34.0	40.0	23.0	41.0
47	34.22218650379062	36.0	33.0	40.0	23.0	41.0
48	34.25367006884728	36.0	33.0	40.0	23.0	41.0
49	34.169774170216186	36.0	33.0	40.0	23.0	41.0
50	34.00406185539393	36.0	33.0	40.0	23.0	41.0
51	32.56844544456053	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	3.0
13	5.0
14	11.0
15	18.0
16	36.0
17	92.0
18	175.0
19	339.0
20	637.0
21	1166.0
22	1750.0
23	2435.0
24	2956.0
25	3265.0
26	3718.0
27	4204.0
28	4563.0
29	5075.0
30	6254.0
31	7838.0
32	10093.0
33	13583.0
34	23536.0
35	30499.0
36	25587.0
37	34825.0
38	51513.0
39	64438.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.924080473626404	19.27221463205551	21.41732969005331	13.38637520426478
2	28.642945163277883	30.745532963647566	24.48766374668488	16.12385812638967
3	29.0608508130408	30.025248466339843	25.905127380856708	15.008773339762651
4	25.230049023547373	26.65052640038576	30.069450025449385	18.04997455061748
5	27.542594229687374	30.195357496852317	24.259623884915214	18.002424388545098
6	23.133153848214523	39.20008572423585	24.172895068177557	13.49386535937207
7	74.85701465348656	8.280090546224116	12.281336226526292	4.581558573763026
8	76.11508478662702	11.465616544777518	7.8725655656460125	4.5467331029494495
9	70.76200809022475	10.211564735192477	10.049492351790832	8.97693482279193
10	42.830306196254924	24.7789922044523	16.71053336547992	15.680168233812852
11	34.049264646789354	26.274813147954674	22.098770392992044	17.577151812263924
12	28.61716092046398	23.703755793083126	27.671850304053148	20.007232982399742
13	24.67518551260414	28.17748935144258	28.245800851884596	18.901524284068685
14	19.84013769455383	33.01622063275202	27.625304722869622	19.518336949824533
15	17.935117468991937	27.130046344665004	33.84131640279675	21.093519783546306
16	20.52794074312197	23.782113102413675	32.170363524337645	23.519582630126713
17	20.245653513354227	26.36522542795146	27.37449436095261	26.0146266977417
18	22.594698491789224	24.89083554341129	28.40485949261968	24.109606472179806
19	23.521926652200705	28.45609311795119	25.853893755525192	22.168086474322912
20	26.18004768410619	26.76638806290016	26.140869029440918	20.91269522355273
21	23.001553751774757	30.916311714752602	26.079589595220874	20.002544938251763
22	20.76602641378017	25.663693107235662	27.79106056953039	25.779219909453776
23	21.430724101904687	29.98372578960058	26.268450802325273	22.317099306169467
24	21.52113638190147	27.43309491280238	28.388116477805458	22.65765222749069
25	20.149883468616895	30.418039593881435	27.148463660960648	22.28361327654103
26	20.18705296150446	28.59807388357577	28.098797181815748	23.11607597310402
27	22.230035629135525	29.515591095395	26.482426531650994	21.77194674381848
28	19.361287470867154	29.27616598355166	30.806477537571325	20.556069008009857
29	21.912922928554206	26.523614348093975	28.66638538401779	22.89707733933403
30	20.942497789922044	29.770419780867424	27.642717458276405	21.644364970934127
31	23.66055881486244	28.082723887594096	23.187401216212596	25.069316081330868
32	25.739706394492217	28.638926839722465	23.968965147740363	21.65240161804495
33	24.625961049050336	26.73457633475314	23.556082402421712	25.083380213774813
34	21.19833105628332	28.34090117602936	25.898765035227306	24.562002732460016
35	23.22289640761874	28.312103190548903	24.27435773795173	24.19064266388063
36	24.748519917490423	28.793297436309572	24.60185110771786	21.856331538482145
37	23.207827694285942	29.84141016367971	26.137855286774357	20.812906855259985
38	22.198893621581075	29.640493985909078	22.779876235634493	25.38073615687535
39	22.42726834364703	30.51447935921134	24.271678855581452	22.786573441560183
40	21.892161590184575	28.180503094109138	26.90502022556189	23.022315090144392
41	21.911583487369068	28.198585550108497	24.51713145275791	25.372699509764523
42	22.101114415066032	27.66515309812746	25.953682123817945	24.28005036298856
43	24.215757186101957	24.70867154223258	26.918414637413274	24.15715663425219
44	23.173671944064935	25.180489699697283	26.806236438158	24.839601918079776
45	21.252243563985104	28.20729191781189	25.42627715717003	25.114187361032975
46	24.108267030994668	29.59696214739211	24.957472742371884	21.33729807924134
47	20.714792788448662	28.317126094993167	28.45040049291436	22.517680623643816
48	23.201130488360256	27.605213105092556	25.532427871092178	23.66122853545501
49	22.406841865573682	25.582991775831122	28.18452141766455	23.825644940930644
50	22.209944011358463	26.81226392349112	27.103927241554825	23.873864823595596
51	21.442109351978356	27.652763267164936	24.529856144016716	26.375271236839993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2601.0
1	2526.5
2	2452.0
3	2524.5
4	2597.0
5	1909.0
6	1221.0
7	1182.5
8	1144.0
9	1168.5
10	1193.0
11	1302.5
12	1412.0
13	1410.0
14	1408.0
15	1271.0
16	1134.0
17	1154.5
18	1175.0
19	1111.0
20	1047.0
21	1037.0
22	1027.0
23	1092.0
24	1157.0
25	1591.5
26	2218.5
27	2411.0
28	3060.5
29	3710.0
30	3937.0
31	4164.0
32	5317.5
33	6471.0
34	6290.0
35	6109.0
36	6668.0
37	7227.0
38	8147.0
39	9067.0
40	9898.5
41	10730.0
42	12966.5
43	15203.0
44	16722.5
45	18242.0
46	24213.5
47	30185.0
48	27193.0
49	24201.0
50	24812.5
51	25424.0
52	22705.0
53	19986.0
54	18643.5
55	17301.0
56	15871.5
57	14442.0
58	13584.0
59	12726.0
60	12013.5
61	11301.0
62	10452.0
63	9603.0
64	8260.5
65	6918.0
66	6170.0
67	5422.0
68	4751.5
69	4081.0
70	4015.5
71	3950.0
72	3425.0
73	2900.0
74	2504.0
75	1741.0
76	1374.0
77	1090.5
78	807.0
79	645.0
80	483.0
81	369.5
82	256.0
83	191.0
84	126.0
85	101.0
86	76.0
87	48.5
88	21.0
89	15.0
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	298632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.264995044067614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37098081599567	21.095194085027728
2	8.545601925769448	4.659915883093573
3	3.1514824003340625	2.5777545607972354
4	1.6580285426543193	1.8082455999357068
5	0.9935889563017367	1.354509898470358
6	0.6853184642971186	1.1211122719601383
7	0.5232001179042519	0.9985534035200515
8	0.44214094470781856	0.9643976532990437
9	0.37090712583822555	0.9101502853009724
>10	4.936012379946452	32.53067320313965
>50	1.1876397042568347	21.69291971389536
>100	0.1215887597946501	5.273379945886576
>500	0.004912677163420206	0.9915213372980793
>1k	0.008597185035985359	4.021672158375526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	2739	0.9171823515229447	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2119	0.7095689678266227	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	1952	0.6536472983471295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	1562	0.5230517827962174	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1300	0.4353183851697072	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.39680945109700233	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1153	0.38609392161590184	No Hit
AAGTGAGGGGTTTTTTCTGCAAGTACTTATCCTTGCTTTTTCTGAATACAT	939	0.3144338182110423	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	925	0.3097457740630609	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	594	0.19890701599292776	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	503	0.16843472903104825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	473	0.15838892014251654	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.14599908917999413	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA	377	0.1262423316992151	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	368	0.12322858903265559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTC	365	0.12222400814380241	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	365	0.12222400814380241	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	336	0.11251305955155509	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	314	0.1051461330332985	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3486029628439017E-4	0.0	0.0	0.13059551555091217	0.0
2	3.3486029628439017E-4	0.0	0.0	0.661683945457955	0.0
3	3.3486029628439017E-4	0.0	0.0	0.8190682847116183	0.0
4	3.3486029628439017E-4	0.0	0.0	1.1602909266254118	0.0
5	3.3486029628439017E-4	0.0	0.0	2.335315706287337	0.0
6	3.3486029628439017E-4	0.0	0.0	2.83894559189906	0.0
7	3.3486029628439017E-4	0.0	0.0	3.3633368158804147	0.0
8	3.3486029628439017E-4	0.0	0.0	4.156955718074419	0.0
9	3.3486029628439017E-4	0.0	0.0	4.4539768008786735	0.0
10	3.3486029628439017E-4	0.0	0.0	6.094792252672185	0.0
11	3.3486029628439017E-4	0.0	0.0	7.005947118862011	0.0
12	3.3486029628439017E-4	0.0	0.0	8.427763936885531	0.0
13	3.3486029628439017E-4	0.0	0.0	8.72210613731951	0.0
14	3.3486029628439017E-4	0.0	0.0	8.872123550054917	0.0
15	3.3486029628439017E-4	0.0	0.0	9.187896809451097	0.0
16	3.3486029628439017E-4	0.0	0.0	9.652013180101262	0.0
17	3.3486029628439017E-4	0.0	0.0	10.17439524230491	0.0
18	3.3486029628439017E-4	0.0	0.0	10.775804334431674	0.0
19	3.3486029628439017E-4	0.0	0.0	11.315933992338396	0.0
20	3.3486029628439017E-4	0.0	0.0	11.654477751881915	0.0
21	3.3486029628439017E-4	0.0	0.0	12.00809022475823	0.0
22	3.3486029628439017E-4	0.0	0.0	12.430683918669132	0.0
23	3.3486029628439017E-4	0.0	0.0	12.820126443247878	0.0
24	3.3486029628439017E-4	0.0	0.0	13.118486967237269	0.0
25	3.3486029628439017E-4	0.0	0.0	13.415508050041522	0.0
26	3.3486029628439017E-4	0.0	0.0	13.67468991936564	0.0
27	3.3486029628439017E-4	0.0	0.0	13.948940502022555	0.0
28	3.3486029628439017E-4	0.0	0.0	14.241608400975114	0.0
29	3.3486029628439017E-4	0.0	0.0	14.518537866002303	0.0
30	3.3486029628439017E-4	0.0	0.0	14.832301963620777	0.0
31	3.3486029628439017E-4	0.0	0.0	15.12697902435104	0.0
32	3.3486029628439017E-4	0.0	0.0	15.443087144043504	0.0
33	3.3486029628439017E-4	0.0	0.0	15.779956602105601	0.0
34	3.3486029628439017E-4	0.0	0.0	16.086018912909534	0.0
35	3.3486029628439017E-4	0.0	0.0	16.43795708430443	0.0
36	3.3486029628439017E-4	0.0	0.0	16.713547108146482	0.0
37	3.3486029628439017E-4	0.0	0.0	16.976077580433444	0.0
38	0.002009161777706341	0.0	0.0	17.29151597953334	0.0
39	0.002009161777706341	0.0	0.0	17.63943582737282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGC	115	0.0	45.000004	45
TATCATG	55	1.8189894E-12	45.0	28
CGTGGTT	20	7.024891E-4	45.0	23
CTAGACT	20	7.024891E-4	45.0	38
CGGGTAG	20	7.024891E-4	45.0	6
TCGTTGC	20	7.024891E-4	45.0	16
CACGGGC	55	1.8189894E-12	45.0	4
TGGACTA	20	7.024891E-4	45.0	23
TAGGTCG	20	7.024891E-4	45.0	1
CCTACTA	20	7.024891E-4	45.0	35
TTACTAG	20	7.024891E-4	45.0	30
ATATGAC	20	7.024891E-4	45.0	36
TAACGCG	20	7.024891E-4	45.0	1
CGACAAA	20	7.024891E-4	45.0	12
CATTCGT	20	7.024891E-4	45.0	20
ACTTACC	20	7.024891E-4	45.0	23
ATCACGC	50	2.1827873E-11	45.0	42
CATTATC	20	7.024891E-4	45.0	42
GCACCGC	20	7.024891E-4	45.0	26
ATCGTGA	20	7.024891E-4	45.0	30
>>END_MODULE