SRR3553623_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705437
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	3365	0.47700928644230456	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	2136	0.30279103591107354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.2751485958349222	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	1939	0.2748650836290129	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1889	0.2677772784812818	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1145	0.1623107378830427	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	1041	0.14756810317576197	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	915	0.12970683420347953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	904	0.1281475170709787	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	711	0.10078858920073655	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	20	7.0314325E-4	45.0	42
TCGATTG	20	7.0314325E-4	45.0	16
CGCGACA	20	7.0314325E-4	45.0	21
TAACGCA	25	3.889268E-5	45.0	1
AATGCGT	25	3.889268E-5	45.0	14
TTCCACG	20	7.0314325E-4	45.0	30
ACGTATA	25	3.889268E-5	45.0	13
TAGGCGA	20	7.0314325E-4	45.0	18
TATCGAT	20	7.0314325E-4	45.0	14
CGTAAGG	140	0.0	43.39286	2
TACTCGC	55	6.002665E-11	40.909092	45
AATTGCG	50	1.0804797E-9	40.5	1
CGAATGC	100	0.0	40.5	45
GCGTATG	50	1.0804797E-9	40.5	1
TAGTGCG	45	1.9264917E-8	40.0	1
GTACGAG	45	1.9264917E-8	40.0	1
GCGTAAG	85	0.0	39.705883	1
CGCACGG	80	0.0	39.375	2
GGCGATA	270	0.0	39.166668	8
ACACGAC	230	0.0	39.130436	26