##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705437
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908137792602314	33.0	31.0	34.0	30.0	34.0
2	32.00368424111579	33.0	31.0	34.0	30.0	34.0
3	31.988186046379763	33.0	31.0	34.0	30.0	34.0
4	35.66383532477032	37.0	35.0	37.0	33.0	37.0
5	35.56416802634396	37.0	35.0	37.0	33.0	37.0
6	35.537984256567206	37.0	35.0	37.0	33.0	37.0
7	35.81529463297218	37.0	35.0	37.0	35.0	37.0
8	35.69169465168399	37.0	35.0	37.0	35.0	37.0
9	37.40214221822785	39.0	37.0	39.0	35.0	39.0
10	36.89854657467641	39.0	37.0	39.0	33.0	39.0
11	36.86604331782994	39.0	37.0	39.0	33.0	39.0
12	36.79718529081973	39.0	35.0	39.0	33.0	39.0
13	36.753949679418575	39.0	35.0	39.0	33.0	39.0
14	37.85528544717671	40.0	37.0	41.0	33.0	41.0
15	37.991041014293266	40.0	37.0	41.0	33.0	41.0
16	38.019973434906305	40.0	37.0	41.0	33.0	41.0
17	37.92785039627918	40.0	37.0	41.0	33.0	41.0
18	37.91306948742411	40.0	37.0	41.0	33.0	41.0
19	37.83491084249905	40.0	36.0	41.0	33.0	41.0
20	37.7062515858964	40.0	36.0	41.0	33.0	41.0
21	37.60702940163331	40.0	36.0	41.0	33.0	41.0
22	37.60806705630694	39.0	36.0	41.0	33.0	41.0
23	37.49062354257007	39.0	35.0	41.0	32.0	41.0
24	37.46951038859601	39.0	35.0	41.0	32.0	41.0
25	37.35759961555745	39.0	35.0	41.0	32.0	41.0
26	37.32892660861282	39.0	35.0	41.0	32.0	41.0
27	37.26976895172779	39.0	35.0	41.0	32.0	41.0
28	37.16580219069881	39.0	35.0	41.0	32.0	41.0
29	37.14959805057007	39.0	35.0	41.0	32.0	41.0
30	37.0048891679909	39.0	35.0	41.0	31.0	41.0
31	36.911415193702624	39.0	35.0	41.0	31.0	41.0
32	36.758983438634495	39.0	35.0	41.0	31.0	41.0
33	36.617777349359336	39.0	35.0	41.0	30.0	41.0
34	36.479786288499184	39.0	35.0	41.0	30.0	41.0
35	36.38558935808584	39.0	35.0	41.0	30.0	41.0
36	36.22002815276205	39.0	35.0	41.0	30.0	41.0
37	36.18188300301799	39.0	35.0	41.0	30.0	41.0
38	36.016727220148645	39.0	35.0	41.0	29.0	41.0
39	35.92227229362792	38.0	35.0	40.0	29.0	41.0
40	35.80940324933339	38.0	35.0	40.0	28.0	41.0
41	35.67069915527538	38.0	35.0	40.0	28.0	41.0
42	35.59036030148688	38.0	35.0	40.0	28.0	41.0
43	35.5415437523124	38.0	35.0	40.0	27.0	41.0
44	35.466447039211154	38.0	34.0	40.0	27.0	41.0
45	35.38901418553322	38.0	34.0	40.0	27.0	41.0
46	35.21157807146492	38.0	34.0	40.0	26.0	41.0
47	35.107832449956554	37.0	34.0	40.0	26.0	41.0
48	35.054156501572784	37.0	34.0	40.0	26.0	41.0
49	34.95368544604266	37.0	34.0	40.0	26.0	41.0
50	34.82793644223368	37.0	34.0	40.0	26.0	41.0
51	33.408477298468895	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	5.0
12	9.0
13	13.0
14	33.0
15	47.0
16	98.0
17	210.0
18	352.0
19	596.0
20	1046.0
21	1866.0
22	2763.0
23	3741.0
24	4746.0
25	5631.0
26	7015.0
27	8378.0
28	9580.0
29	11298.0
30	13735.0
31	17207.0
32	21679.0
33	30115.0
34	48696.0
35	59802.0
36	60744.0
37	85299.0
38	132701.0
39	177975.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.66196130908926	18.566080316172812	22.79990984311852	13.972048531619407
2	30.578917748856384	27.476727191797423	24.58461917931722	17.359735880028975
3	32.333716547331655	26.419793688167758	25.58073931477935	15.665750449721235
4	27.843166717935127	26.593303158184217	26.499318861925303	19.064211261955354
5	26.246425974254254	30.406825839869473	24.011499255071676	19.335248930804593
6	23.41938401303022	38.33609521473924	23.789083929535877	14.455436842694668
7	79.49256985386364	6.681106888354312	9.874304863510137	3.9520183942719194
8	80.93408766480918	7.744135904411024	7.343816669667171	3.977959761112615
9	75.61752502349609	8.13467396805101	9.20592483807909	7.041876170373825
10	40.63495393635434	26.508674764720308	16.76932171122297	16.087049587702374
11	31.833742772210698	26.607053500170817	22.04675966812061	19.51244405949787
12	27.229504548244567	23.393726158395435	28.497654645276615	20.879114648083387
13	25.159298420695258	24.911792264936487	29.88076894180487	20.048140372563388
14	21.41225935129572	29.274052821159085	27.38061655399419	21.933071273551004
15	20.451578241572246	26.360539637132728	31.873717993243904	21.31416412805112
16	23.073215609615033	24.662159767633398	29.975462018578554	22.289162604173015
17	23.334755619566312	24.46823741879147	27.946932185297907	24.250074776344306
18	23.189880882346685	25.36115911130264	28.052823994205013	23.396136012145664
19	24.459448540408285	26.720741894740424	25.8614163986295	22.95839316622179
20	25.691167318981	26.475220324423017	26.670418478191532	21.16319387840445
21	25.204235105331875	27.432499287675583	26.611164427156503	20.752101179836043
22	23.400813963543165	25.479950725578615	25.831789373111985	25.287445937766233
23	23.376290157732015	27.84345023014103	25.923080303414764	22.85717930871219
24	22.6428440810448	27.153381520957932	27.13651254470633	23.067261853290937
25	22.93727150688155	28.16112565686234	26.412847639122983	22.488755197133127
26	21.586902870135816	27.18995459552022	27.31058903913461	23.91255349520935
27	23.42207737898636	26.5115098867794	27.688510809611632	22.37790192462261
28	20.83871415874132	28.1259701433296	28.895422270167288	22.1398934277618
29	22.899564383495623	26.03492586864596	27.64598397872524	23.419525769133177
30	22.63277939773502	27.544203096803827	26.278888121830867	23.54412938363029
31	23.914396324547763	27.961533063902234	24.336687755249585	23.78738285630042
32	24.909240655083302	28.09917823987117	24.618215375717465	22.37336572932806
33	23.501177284435038	27.422434604365804	24.32619780363094	24.750190307568218
34	21.783660341036832	28.132632680168463	25.95072274349091	24.132984235303788
35	23.339717083169724	25.617595901547553	25.492992287050438	25.549694728232286
36	23.23439229867444	29.17312247585539	24.452077223054648	23.140408002415526
37	23.12197970903142	28.602979429771903	25.11563754098523	23.159403320211442
38	22.681118228842546	28.944044613480724	24.89634084971443	23.4784963079623
39	23.57730031172167	27.414212750394434	25.204660373640735	23.80382656424316
40	22.785308964514194	26.215948412119012	27.451494605471506	23.54724801789529
41	20.77435688799992	26.63653876958538	26.535750180384643	26.05335416203006
42	20.80100703535539	26.589475743404446	26.72343526069656	25.886081960543606
43	22.310284263513257	25.38384008777538	26.366209881250914	25.93966576746045
44	22.69260047318187	27.045363370506507	25.860282349805864	24.401753806505756
45	22.963779898134064	26.02471942923323	26.50229574008735	24.50920493254536
46	23.319871228756075	28.00165004103839	25.485337457490886	23.193141272714644
47	21.43848423034233	27.397343774142836	28.081458727001845	23.082713268512993
48	22.519799783680185	27.113831568233593	26.215097875501286	24.151270772584937
49	22.1108334266561	26.692957698561315	26.9269970245394	24.269211850243185
50	21.71760199705998	25.868220691571324	27.945514624268363	24.468662687100338
51	22.09183810886018	26.055622259677335	25.97000157349274	25.88253805796974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2444.0
1	2621.5
2	2799.0
3	3510.5
4	4222.0
5	3037.5
6	1853.0
7	1887.5
8	1922.0
9	1826.0
10	1730.0
11	1857.5
12	1985.0
13	2046.0
14	2107.0
15	2112.5
16	2118.0
17	2095.0
18	2072.0
19	2042.0
20	2012.0
21	2399.5
22	2787.0
23	3013.0
24	3239.0
25	4192.5
26	6091.5
27	7037.0
28	7970.0
29	8903.0
30	10466.0
31	12029.0
32	13189.0
33	14349.0
34	16019.0
35	17689.0
36	18671.5
37	19654.0
38	22497.0
39	25340.0
40	27194.5
41	29049.0
42	32230.0
43	35411.0
44	38232.5
45	41054.0
46	49013.0
47	56972.0
48	56370.5
49	55769.0
50	54466.5
51	53164.0
52	49551.5
53	45939.0
54	43030.0
55	40121.0
56	37452.5
57	34784.0
58	33161.0
59	31538.0
60	31950.5
61	32363.0
62	29384.5
63	26406.0
64	23889.0
65	21372.0
66	19417.5
67	17463.0
68	14408.0
69	11353.0
70	10433.0
71	9513.0
72	8844.0
73	8175.0
74	6752.0
75	4566.0
76	3803.0
77	2846.5
78	1890.0
79	1546.0
80	1202.0
81	968.5
82	735.0
83	497.5
84	260.0
85	206.0
86	152.0
87	102.5
88	53.0
89	40.5
90	28.0
91	52.0
92	76.0
93	49.0
94	22.0
95	12.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.201895738290464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00847897496212	18.903558666147475
2	8.809731506535412	4.440438698200761
3	3.1235024763747656	2.3615455124366673
4	1.6675081495339361	1.680974661092157
5	1.0662105809417664	1.3435263947978247
6	0.8305498692865947	1.2558858726726918
7	0.6258748334383303	1.1041262608272897
8	0.5321488562185729	1.0728927993336796
9	0.4827586366435473	1.0949789544704935
>10	6.542075669491421	40.06107944899641
>50	1.2087348786712324	19.85304880722918
>100	0.09223959982369408	3.8953664668744405
>500	0.006224758270310643	1.013559693932659
>1k	0.003961209808379501	1.9190177629883016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	3365	0.47700928644230456	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	2136	0.30279103591107354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.2751485958349222	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	1939	0.2748650836290129	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1889	0.2677772784812818	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1145	0.1623107378830427	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	1041	0.14756810317576197	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	915	0.12970683420347953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	904	0.1281475170709787	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	711	0.10078858920073655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4175610295462246E-4	0.0	0.0	0.0713033197861751	0.0
2	2.8351220590924493E-4	0.0	0.0	0.3662977700347444	0.0
3	2.8351220590924493E-4	0.0	0.0	0.49997377512095337	0.0
4	2.8351220590924493E-4	0.0	0.0	0.7368482231581275	0.0
5	2.8351220590924493E-4	0.0	0.0	1.5381954731606082	0.0
6	2.8351220590924493E-4	0.0	0.0	2.0096762715876824	0.0
7	2.8351220590924493E-4	0.0	0.0	2.4759120942054356	0.0
8	2.8351220590924493E-4	0.0	0.0	3.13139231426761	0.0
9	2.8351220590924493E-4	0.0	0.0	3.3993113488518465	0.0
10	2.8351220590924493E-4	0.0	0.0	4.468577633438564	0.0
11	2.8351220590924493E-4	0.0	0.0	5.27715444469173	0.0
12	2.8351220590924493E-4	0.0	0.0	6.168516820070396	0.0
13	2.8351220590924493E-4	0.0	0.0	6.425804146933036	0.0
14	2.8351220590924493E-4	0.0	0.0	6.538074980473096	0.0
15	2.8351220590924493E-4	0.0	0.0	6.768428647774359	0.0
16	2.8351220590924493E-4	0.0	0.0	7.145925149942518	0.0
17	2.8351220590924493E-4	0.0	0.0	7.586644874028439	0.0
18	2.8351220590924493E-4	0.0	0.0	8.152251724817383	0.0
19	4.2526830886386734E-4	0.0	0.0	8.497427835511889	0.0
20	4.2526830886386734E-4	0.0	0.0	8.787744334362955	0.0
21	4.2526830886386734E-4	0.0	0.0	9.11236581012904	0.0
22	4.2526830886386734E-4	0.0	0.0	9.472851579942645	0.0
23	4.2526830886386734E-4	0.0	0.0	9.841700959830574	0.0
24	4.2526830886386734E-4	0.0	0.0	10.129324092725502	0.0
25	4.2526830886386734E-4	0.0	0.0	10.390438834367917	0.0
26	4.2526830886386734E-4	0.0	0.0	10.625328696963726	0.0
27	4.2526830886386734E-4	0.0	0.0	10.874535925957952	0.0
28	4.2526830886386734E-4	0.0	0.0	11.131256228408773	0.0
29	4.2526830886386734E-4	0.0	0.0	11.397757701963464	0.0
30	4.2526830886386734E-4	0.0	0.0	11.691334591182487	0.0
31	4.2526830886386734E-4	0.0	0.0	11.960529430693315	0.0
32	4.2526830886386734E-4	0.0	0.0	12.22929900189528	0.0
33	4.2526830886386734E-4	0.0	0.0	12.518764964128618	0.0
34	4.2526830886386734E-4	0.0	0.0	12.78314009613899	0.0
35	4.2526830886386734E-4	0.0	0.0	13.095712303153931	0.0
36	5.670244118184899E-4	0.0	0.0	13.366182947591351	0.0
37	5.670244118184899E-4	0.0	0.0	13.655507153721736	0.0
38	5.670244118184899E-4	0.0	0.0	13.928387651909384	0.0
39	5.670244118184899E-4	0.0	0.0	14.196448442596575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	20	7.0314325E-4	45.0	42
TCGATTG	20	7.0314325E-4	45.0	16
CGCGACA	20	7.0314325E-4	45.0	21
TAACGCA	25	3.889268E-5	45.0	1
AATGCGT	25	3.889268E-5	45.0	14
TTCCACG	20	7.0314325E-4	45.0	30
ACGTATA	25	3.889268E-5	45.0	13
TAGGCGA	20	7.0314325E-4	45.0	18
TATCGAT	20	7.0314325E-4	45.0	14
CGTAAGG	140	0.0	43.39286	2
TACTCGC	55	6.002665E-11	40.909092	45
AATTGCG	50	1.0804797E-9	40.5	1
CGAATGC	100	0.0	40.5	45
GCGTATG	50	1.0804797E-9	40.5	1
TAGTGCG	45	1.9264917E-8	40.0	1
GTACGAG	45	1.9264917E-8	40.0	1
GCGTAAG	85	0.0	39.705883	1
CGCACGG	80	0.0	39.375	2
GGCGATA	270	0.0	39.166668	8
ACACGAC	230	0.0	39.130436	26
>>END_MODULE