FastQCFastQC Report
Sat 18 Jun 2016
SRR3553622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316053
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG61020.4636591383477717No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC54850.41677652799697273No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC42110.3199719160246586No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC36140.2746090013092178No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35300.26822627964071355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20610.15660463522365742No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16180.12294337690047438No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC14960.11367323352478965No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC14400.10941808574578683No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT14360.1091141466187152No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13560.1030353640772826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA302.1657434E-645.00000413
CGTAAGC253.891116E-545.043
TTACGAG207.03366E-445.01
TTCGATC253.891116E-545.021
TATCGAT453.8562575E-1045.013
CGATCGA1250.044.99999641
CCGATCG1350.043.33333640
CCCTCGT5900.041.567814
ACTATGC6100.040.5737728
GCGAACC501.0822987E-940.533
TACGAAT1400.040.1785712
CGAATAT1400.040.1785714
AGTTTCG451.9286745E-840.01
CGTACAT451.9286745E-840.035
ACGTAAG403.4587356E-739.3751
ACGTAGG1900.039.078952
CGTGTAC6250.038.8818
CCGATGA8300.038.49397318
CGTAAGG1950.038.0769232
ACGCATT1250.037.817