##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316053
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.935734351124157	33.0	31.0	34.0	30.0	34.0
2	32.03796731590597	33.0	31.0	34.0	30.0	34.0
3	32.01381783256449	33.0	31.0	34.0	30.0	34.0
4	35.68387975256316	37.0	35.0	37.0	33.0	37.0
5	35.587742286974766	37.0	35.0	37.0	33.0	37.0
6	35.560112700628316	37.0	35.0	37.0	33.0	37.0
7	35.847609480773194	37.0	35.0	37.0	35.0	37.0
8	35.73263082869763	37.0	35.0	37.0	35.0	37.0
9	37.426960008449505	39.0	37.0	39.0	35.0	39.0
10	36.94091271400164	39.0	37.0	39.0	33.0	39.0
11	36.915137916178146	39.0	37.0	39.0	33.0	39.0
12	36.85145507057847	39.0	37.0	39.0	33.0	39.0
13	36.85152497657769	39.0	37.0	39.0	33.0	39.0
14	37.94635018498495	40.0	37.0	41.0	33.0	41.0
15	38.05408748735803	40.0	37.0	41.0	33.0	41.0
16	38.0891878974479	40.0	37.0	41.0	33.0	41.0
17	37.989034636143074	40.0	37.0	41.0	33.0	41.0
18	37.971410725859826	40.0	37.0	41.0	33.0	41.0
19	37.90537159217752	40.0	37.0	41.0	33.0	41.0
20	37.786642331273896	40.0	36.0	41.0	33.0	41.0
21	37.689960054800224	40.0	36.0	41.0	33.0	41.0
22	37.67230651045209	40.0	36.0	41.0	33.0	41.0
23	37.5849802401575	39.0	36.0	41.0	33.0	41.0
24	37.55341692165893	39.0	36.0	41.0	33.0	41.0
25	37.430394520585416	39.0	35.0	41.0	32.0	41.0
26	37.37384512629811	39.0	35.0	41.0	32.0	41.0
27	37.30300679379934	39.0	35.0	41.0	32.0	41.0
28	37.180766276130214	39.0	35.0	41.0	32.0	41.0
29	37.14020332007905	39.0	35.0	41.0	32.0	41.0
30	36.99807758502127	39.0	35.0	41.0	31.0	41.0
31	36.94443004954967	39.0	35.0	41.0	31.0	41.0
32	36.79511995337574	39.0	35.0	41.0	31.0	41.0
33	36.697569170846464	39.0	35.0	41.0	31.0	41.0
34	36.54084144027634	39.0	35.0	41.0	30.0	41.0
35	36.456502131753055	39.0	35.0	41.0	30.0	41.0
36	36.290564285784846	39.0	35.0	41.0	30.0	41.0
37	36.22604028865099	39.0	35.0	41.0	30.0	41.0
38	36.07157538488192	39.0	35.0	40.0	29.0	41.0
39	35.97800240567819	38.0	35.0	40.0	29.0	41.0
40	35.84624251454919	38.0	35.0	40.0	29.0	41.0
41	35.698122340057736	38.0	35.0	40.0	28.0	41.0
42	35.590878178918324	38.0	35.0	40.0	28.0	41.0
43	35.50510655725871	38.0	34.0	40.0	27.0	41.0
44	35.44425870386679	38.0	34.0	40.0	27.0	41.0
45	35.37825376333628	38.0	34.0	40.0	27.0	41.0
46	35.211290122814205	38.0	34.0	40.0	27.0	41.0
47	35.092161181958474	37.0	34.0	40.0	26.0	41.0
48	35.015553324980075	37.0	34.0	40.0	26.0	41.0
49	34.912332558035274	37.0	34.0	40.0	26.0	41.0
50	34.79185336760754	37.0	34.0	40.0	26.0	41.0
51	33.29538779973147	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	13.0
11	9.0
12	17.0
13	24.0
14	46.0
15	100.0
16	144.0
17	305.0
18	589.0
19	1149.0
20	1969.0
21	3101.0
22	4833.0
23	6519.0
24	8172.0
25	10418.0
26	12933.0
27	15279.0
28	17647.0
29	20948.0
30	25541.0
31	32096.0
32	41130.0
33	55578.0
34	89745.0
35	108232.0
36	115869.0
37	163040.0
38	250381.0
39	330124.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92513523391535	19.284329734440785	24.023196634178106	14.767338397465757
2	30.400599367958588	27.575029273137176	24.797861484301926	17.226509874602314
3	31.747125685667676	25.820388692552655	26.50326392630084	15.929221695478828
4	28.410253994330013	25.35247440642588	27.027786874844708	19.2094847243994
5	25.730802634848292	29.30543070833773	24.522188696047955	20.441577960766015
6	23.864160485937877	36.710223676402094	24.659037288011955	14.766578549648077
7	80.36895170635225	6.325277173487693	9.270371330030022	4.0353997901300325
8	81.8879634786745	6.8925035693851235	7.451675578415156	3.7678573735252305
9	76.20969672194053	7.1599700012081575	9.11756593389476	7.512767342956553
10	41.331694088308	24.768835297666584	16.98844955332346	16.911021060701962
11	31.428977404405444	25.773582066983625	22.206932395579813	20.590508133031115
12	25.815525666519513	22.65850995362649	29.863083021732407	21.662881358121595
13	25.005147968964774	23.557865830631442	30.77809176378155	20.658894436622234
14	22.034446940966664	27.08758689809605	28.647478483009426	22.230487677927865
15	21.49130772088966	25.145643830453636	30.98332665933667	22.379721789320033
16	23.995310219269285	23.776626017341247	28.95810427087663	23.26995949251284
17	23.347539954697876	24.06840757933001	27.892721645708797	24.691330820263317
18	24.422344692804927	24.17516619771392	27.347758790869364	24.05473031861179
19	25.440692738058424	25.468883092094313	25.36197250414687	23.728451665700394
20	26.11771714361048	24.8452759881251	26.665415450593557	22.37159141767087
21	26.147275223718193	26.136941293397758	27.040628302963484	20.675155179920566
22	23.218213856128894	23.752082932830213	27.007498938112672	26.022204272928217
23	23.84774777307601	26.06353999420996	26.253805887756798	23.834906344957233
24	23.428387762498925	25.638481125000283	27.06653911354634	23.86659199895445
25	24.011798916912923	27.312197913001985	25.547223402097032	23.128779767988068
26	22.588831908745316	25.655197776989226	26.582592038466537	25.173378275798925
27	24.669371218332394	24.222960625445936	27.01304582718173	24.09462232903994
28	22.48518866641389	25.989606801549787	28.642007578722133	22.88319695331419
29	23.863400638120197	25.290546809285036	27.098756661015933	23.747295891578833
30	23.409467551838716	25.487955272318057	27.096629087126427	24.005948088716792
31	24.998537292950967	25.664087996456068	25.242980335898324	24.094394374694637
32	25.03660566861669	26.687147098179175	25.20977498626575	23.066472246938382
33	25.11129870909454	24.859941051006302	24.024184436341088	26.004575803558062
34	23.845620199186506	25.505583741688216	26.33518558903023	24.31361047009505
35	25.465311807351227	24.091582937769225	25.553758093329066	24.889347161550486
36	25.14070481963872	27.03454952042205	25.12482400024923	22.69992165969
37	24.038165636186385	26.660020531088037	26.36953071038932	22.93228312233626
38	24.646347829456715	27.274357491681567	25.37572574964686	22.703568929214857
39	25.8437160205554	25.36774734756123	25.230290877343087	23.55824575454028
40	25.503076243889872	24.174330365114475	27.65587708093823	22.66671631005742
41	22.812987014960644	25.831406485909003	26.85598528326747	24.499621215862888
42	23.097701992244993	26.22196826419605	27.217824814046242	23.46250492951272
43	23.2572700339576	23.853066707799762	27.62525521388576	25.264408044356877
44	23.74281278945453	24.448407472951317	27.19457347082526	24.614206266768893
45	25.19503393860278	23.635446292816475	25.9741818908509	25.195337877729852
46	24.862068624895805	25.260608805268483	26.09742920687845	23.779893362957267
47	23.7267800005015	25.21311831666354	28.09560101302911	22.96450066980585
48	23.155070502479763	26.245675516107635	26.62377579018474	23.97547819122786
49	23.289791520554264	25.920764589268064	27.899636260849675	22.889807629328
50	22.241505471284214	24.842540535981453	28.07500913717001	24.840944855564327
51	22.72788405938059	24.662684557536814	26.22265212723196	26.38677925585064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2726.0
1	3265.5
2	3805.0
3	5434.0
4	7063.0
5	5074.5
6	3086.0
7	3062.5
8	3039.0
9	3182.0
10	3325.0
11	3354.5
12	3384.0
13	3404.0
14	3424.0
15	3750.5
16	4077.0
17	3670.5
18	3264.0
19	3340.0
20	3416.0
21	4184.0
22	4952.0
23	4835.0
24	4718.0
25	6204.5
26	9333.0
27	10975.0
28	13237.0
29	15499.0
30	18093.0
31	20687.0
32	22977.0
33	25267.0
34	26871.0
35	28475.0
36	31963.5
37	35452.0
38	37652.5
39	39853.0
40	43430.5
41	47008.0
42	52500.5
43	57993.0
44	63758.0
45	69523.0
46	86733.5
47	103944.0
48	106546.0
49	109148.0
50	106037.0
51	102926.0
52	95160.5
53	87395.0
54	81646.5
55	75898.0
56	71623.0
57	67348.0
58	66916.0
59	66484.0
60	64252.5
61	62021.0
62	58492.5
63	54964.0
64	49890.0
65	44816.0
66	40134.0
67	35452.0
68	31127.0
69	26802.0
70	24904.5
71	23007.0
72	19752.5
73	16498.0
74	14768.0
75	10652.0
76	8266.0
77	6267.5
78	4269.0
79	3316.5
80	2364.0
81	1942.0
82	1520.0
83	1093.5
84	667.0
85	431.0
86	195.0
87	191.5
88	188.0
89	120.5
90	53.0
91	44.5
92	36.0
93	34.0
94	32.0
95	23.0
96	14.0
97	9.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1316053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.453214725593444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09179304185591	17.37690731614877
2	9.38645853346041	4.402852549962518
3	3.47953127310758	2.448185822778288
4	1.7747880276044965	1.6649793881528288
5	1.1595511237616298	1.3597600745442342
6	0.7714390297197546	1.0855635127032521
7	0.6236223107317821	1.023816357288427
8	0.5081191644055542	0.9533622295194062
9	0.4122917402929382	0.8702610043210997
>10	6.059028128966167	35.04728190503836
>50	1.5703938878772847	24.45807298882739
>100	0.1518786338090188	5.331343048502117
>500	0.004899310768011714	0.8101539445779152
>1k	0.005552552203746609	2.279860221116482
>5k	6.532414357348951E-4	0.8875996365189395
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	6102	0.4636591383477717	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	5485	0.41677652799697273	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4211	0.3199719160246586	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	3614	0.2746090013092178	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3530	0.26822627964071355	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2061	0.15660463522365742	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1618	0.12294337690047438	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	1496	0.11367323352478965	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1440	0.10941808574578683	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	1436	0.1091141466187152	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1356	0.1030353640772826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.039391270716301E-4	0.0	0.0	0.04870624511322872	0.0
2	3.039391270716301E-4	0.0	0.0	0.3211116877511772	0.0
3	3.039391270716301E-4	0.0	0.0	0.46137959489473446	0.0
4	3.039391270716301E-4	0.0	0.0	0.7613675133144334	0.0
5	3.039391270716301E-4	0.0	0.0	1.8221910515761903	0.0
6	3.039391270716301E-4	0.0	0.0	2.4443544446918173	0.0
7	3.039391270716301E-4	0.0	0.0	2.997523655962184	0.0
8	3.039391270716301E-4	0.0	0.0	3.709197122000406	0.0
9	3.039391270716301E-4	0.0	0.0	4.005309816549941	0.0
10	3.039391270716301E-4	0.0	0.0	5.110052558673549	0.0
11	3.799239088395376E-4	0.0	0.0	6.0512760504326195	0.0
12	3.799239088395376E-4	0.0	0.0	7.051539717625354	0.0
13	3.799239088395376E-4	0.0	0.0	7.340965751379314	0.0
14	3.799239088395376E-4	0.0	0.0	7.4623894326444296	0.0
15	4.5590869060744513E-4	0.0	0.0	7.689052036658098	0.0
16	4.5590869060744513E-4	0.0	0.0	8.094430847389885	0.0
17	4.5590869060744513E-4	0.0	0.0	8.556950214011138	0.0
18	4.5590869060744513E-4	0.0	0.0	9.089679518985937	0.0
19	4.5590869060744513E-4	0.0	0.0	9.45782578665145	0.0
20	4.5590869060744513E-4	0.0	0.0	9.764348396303188	0.0
21	4.5590869060744513E-4	0.0	0.0	10.102404690388608	0.0
22	4.5590869060744513E-4	0.0	0.0	10.47275451672539	0.0
23	4.5590869060744513E-4	0.0	0.0	10.816813608570476	0.0
24	5.318934723753527E-4	0.0	0.0	11.10965895750399	0.0
25	6.078782541432602E-4	0.0	0.0	11.366487519879518	0.0
26	6.078782541432602E-4	0.0	0.0	11.609942760663895	0.0
27	6.078782541432602E-4	0.0	0.0	11.863276023078098	0.0
28	6.078782541432602E-4	0.0	0.0	12.126335337558594	0.0
29	6.078782541432602E-4	0.0	0.0	12.393193891127485	0.0
30	6.078782541432602E-4	0.0	0.0	12.696449155163204	0.0
31	6.078782541432602E-4	0.0	0.0	12.964599450022149	0.0
32	6.078782541432602E-4	0.0	0.0	13.244679355618656	0.0
33	6.838630359111678E-4	0.0	0.0	13.519060402582571	0.0
34	8.358325994469827E-4	0.0	0.0	13.785311077897319	0.0
35	8.358325994469827E-4	0.0	0.0	14.086210813698232	0.0
36	8.358325994469827E-4	0.0	0.0	14.361351708479825	0.0
37	9.118173812148903E-4	0.0	0.0	14.636188664134346	0.0
38	9.118173812148903E-4	0.0	0.0	14.899475932960147	0.0
39	9.87802162982798E-4	0.0	0.0	15.156000556208603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATA	30	2.1657434E-6	45.000004	13
CGTAAGC	25	3.891116E-5	45.0	43
TTACGAG	20	7.03366E-4	45.0	1
TTCGATC	25	3.891116E-5	45.0	21
TATCGAT	45	3.8562575E-10	45.0	13
CGATCGA	125	0.0	44.999996	41
CCGATCG	135	0.0	43.333336	40
CCCTCGT	590	0.0	41.5678	14
ACTATGC	610	0.0	40.573772	8
GCGAACC	50	1.0822987E-9	40.5	33
TACGAAT	140	0.0	40.17857	12
CGAATAT	140	0.0	40.17857	14
AGTTTCG	45	1.9286745E-8	40.0	1
CGTACAT	45	1.9286745E-8	40.0	35
ACGTAAG	40	3.4587356E-7	39.375	1
ACGTAGG	190	0.0	39.07895	2
CGTGTAC	625	0.0	38.88	18
CCGATGA	830	0.0	38.493973	18
CGTAAGG	195	0.0	38.076923	2
ACGCATT	125	0.0	37.8	17
>>END_MODULE