FastQCFastQC Report
Sat 18 Jun 2016
SRR3553620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373626
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24920.6669771375653728No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG14280.38220038220038216No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13790.36908566320331027No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.3259944436415025No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC9580.2564061387590799No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC8730.2336561160090572No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC8590.2299090534384652No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC8380.22428845958257726No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC8160.21840021840021842No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC6160.1648707531060472No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6150.16460310577957638No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780.1279354220530691No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT4720.12632953809424396Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4460.11937070760600173No Hit
GTGAATGGGGGTTCAGTTTCATCAACGCTCGATGGACCAGGAATCTGATGA4190.11214422979128863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT900.045.00000416
GACAGTC207.027167E-445.038
AACGTAC207.027167E-445.021
AACGTAA207.027167E-445.023
CGGAACA207.027167E-445.019
ACGCCTA253.885735E-545.017
GTCGATG351.2089913E-745.01
GTACATC207.027167E-445.036
TAGCGCG253.885735E-545.01
ACTCTAC253.885735E-545.045
TTGTCGG207.027167E-445.02
CGACCGA207.027167E-445.011
AAGGTTC253.885735E-545.05
ATAGACG207.027167E-445.01
TGTGCGT207.027167E-445.039
TCGATAG207.027167E-445.01
CCGTCGA207.027167E-445.020
CCGTCAA253.885735E-545.013
AGTATCG207.027167E-445.01
CGTTAGG351.2089913E-745.02