##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373626
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854996707937886	33.0	31.0	34.0	30.0	34.0
2	31.932758962170727	33.0	31.0	34.0	30.0	34.0
3	31.917859035506094	33.0	31.0	34.0	30.0	34.0
4	35.62355403531874	37.0	35.0	37.0	33.0	37.0
5	35.527214380155556	37.0	35.0	37.0	33.0	37.0
6	35.51041951041951	37.0	35.0	37.0	33.0	37.0
7	35.77172894819954	37.0	35.0	37.0	35.0	37.0
8	35.638842050606755	37.0	35.0	37.0	35.0	37.0
9	37.380401792166495	39.0	37.0	39.0	35.0	39.0
10	36.90097316567905	39.0	37.0	39.0	33.0	39.0
11	36.840037898861425	39.0	37.0	39.0	33.0	39.0
12	36.70066055360173	39.0	35.0	39.0	33.0	39.0
13	36.6357212827801	39.0	35.0	39.0	32.0	39.0
14	37.71418209653504	40.0	36.0	41.0	33.0	41.0
15	37.85262802909862	40.0	36.0	41.0	33.0	41.0
16	37.927863692569574	40.0	36.0	41.0	33.0	41.0
17	37.84399640281993	40.0	36.0	41.0	33.0	41.0
18	37.79795838619368	39.0	36.0	41.0	33.0	41.0
19	37.65853554088848	39.0	36.0	41.0	33.0	41.0
20	37.55865758806935	39.0	35.0	41.0	33.0	41.0
21	37.437592672886794	39.0	35.0	41.0	33.0	41.0
22	37.43733037850685	39.0	35.0	41.0	33.0	41.0
23	37.34734199440082	39.0	35.0	41.0	32.0	41.0
24	37.326955297543535	39.0	35.0	41.0	32.0	41.0
25	37.201463495581145	39.0	35.0	41.0	32.0	41.0
26	37.148439883734	39.0	35.0	41.0	32.0	41.0
27	37.100903042079516	39.0	35.0	41.0	32.0	41.0
28	37.03394303394303	39.0	35.0	41.0	32.0	41.0
29	36.98128609893316	39.0	35.0	41.0	32.0	41.0
30	36.85392344215874	39.0	35.0	41.0	31.0	41.0
31	36.76472461766579	39.0	35.0	41.0	31.0	41.0
32	36.55069775658011	39.0	35.0	41.0	31.0	41.0
33	36.42327086444733	39.0	35.0	41.0	30.0	41.0
34	36.29601794307677	39.0	35.0	41.0	30.0	41.0
35	36.208920150096624	39.0	35.0	41.0	30.0	41.0
36	36.01119301119301	38.0	35.0	40.0	30.0	41.0
37	35.94362009067891	38.0	35.0	40.0	29.0	41.0
38	35.831896067190186	38.0	35.0	40.0	29.0	41.0
39	35.72430719489543	38.0	35.0	40.0	29.0	41.0
40	35.56550133020721	38.0	35.0	40.0	28.0	41.0
41	35.41702932879404	38.0	34.0	40.0	27.0	41.0
42	35.286604786604784	38.0	34.0	40.0	27.0	41.0
43	35.214516655693124	38.0	34.0	40.0	26.0	41.0
44	35.09718542071483	38.0	34.0	40.0	26.0	41.0
45	35.02658540893835	37.0	34.0	40.0	26.0	41.0
46	34.82001252589488	37.0	34.0	40.0	25.0	41.0
47	34.71160732925439	37.0	34.0	40.0	25.0	41.0
48	34.65677977442683	37.0	34.0	40.0	24.0	41.0
49	34.59352935823524	37.0	34.0	40.0	24.0	41.0
50	34.44048594048594	36.0	34.0	40.0	24.0	41.0
51	32.95110886287357	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	3.0
11	3.0
12	3.0
13	1.0
14	11.0
15	21.0
16	51.0
17	94.0
18	178.0
19	373.0
20	637.0
21	1040.0
22	1518.0
23	2105.0
24	2765.0
25	3241.0
26	4019.0
27	4628.0
28	5469.0
29	6244.0
30	7496.0
31	9351.0
32	12332.0
33	16741.0
34	27800.0
35	36223.0
36	32254.0
37	44468.0
38	68750.0
39	85765.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95859495859496	19.1349103113809	22.510478392831335	13.396016337192806
2	30.432839256368666	27.451783334136277	24.369556722497897	17.745820686997156
3	30.807545513427865	26.876341582223933	26.696750226161992	15.619362678186208
4	26.213111507229154	28.30343712696654	26.669717846188433	18.813733519615873
5	25.05152211034564	31.541166835284486	24.17524476348006	19.232066290889822
6	23.360258654376302	38.12823518705872	24.454400924989162	14.057105233575824
7	78.02374567080449	8.38057308645544	9.216970981676864	4.378710261063203
8	79.17275564334389	9.125703243350303	7.564248740719329	4.13729237258649
9	74.3079978374096	9.6133566721802	9.619512560689031	6.459132929721164
10	40.38771391712569	29.01992901992902	16.00771894889542	14.584638114049877
11	31.577031577031576	26.789356201120906	22.495490142548967	19.13812207929855
12	26.204546792782086	24.74131885896592	29.10504086974675	19.94909347850524
13	23.998865175335762	28.074331015507486	29.393297040355865	18.533506768800887
14	19.833469833469834	31.069304598716364	27.519765755059872	21.57745981275393
15	17.86867080984728	27.438133320486262	33.13768313768314	21.55551273198332
16	20.13805249099367	25.980525980525982	31.989476107123167	21.891945421357185
17	20.68672951025892	26.11568788039376	28.216719393189983	24.980863216157335
18	21.58040393334511	26.201335024864434	28.91554656260539	23.30271447918507
19	23.15657903893198	28.50096085390203	25.704046292281586	22.6384138148844
20	26.19972914090561	26.3991263991264	26.07286430815843	21.328280151809565
21	23.395588101470455	29.7575650516827	25.828502299090534	21.018344547756314
22	21.841895371307135	25.82100817394935	26.009967186437777	26.32712926830574
23	20.722058957353077	30.291253820665588	24.896554308319015	24.090132913662327
24	20.62062062062062	28.185137008666423	28.98513486748781	22.20910750322515
25	21.215332980038863	29.797176856000384	26.907388672094555	22.0801014918662
26	19.634340222575517	29.9770358593888	27.403874462697992	22.984749455337692
27	21.985621985621986	28.62916392328157	26.57497069261775	22.810243398478693
28	18.67589514648338	29.312735195088134	29.871850460085753	22.13951919834273
29	20.05401123048182	27.96887796887797	28.809022926669986	23.16808787397023
30	21.993651405416113	28.65592865592866	26.80032974150621	22.550090197149018
31	24.98327204209557	27.748871866518925	24.037674037674037	23.230182053711466
32	23.621750092338328	29.152949741185036	25.591902062490295	21.63339810398634
33	23.690000160588394	28.647096294155116	23.157917275564337	24.504986269692154
34	21.948419007242535	28.754958166722872	25.399998929410692	23.896623896623897
35	23.631117748764808	27.433583315936254	25.35824594648124	23.577052988817695
36	24.30478606949195	29.98399468987704	24.011444599679894	21.699774640951112
37	23.30030565324683	28.361516596810716	27.038803509391744	21.299374240550712
38	21.770433535139418	30.527854057265824	25.47681371210783	22.22489869548693
39	23.87601505248564	28.454657866422572	25.508128449304916	22.161198631786867
40	24.931348460760226	27.186009538950717	26.646164881459	21.23647711883006
41	22.152098622686857	27.125521243168304	25.76801400330812	24.954366130836718
42	22.511816629463688	28.318693024575374	25.46021957786664	23.7092707680943
43	22.94674353497883	27.29413905884494	25.85767585767586	23.901441548500372
44	22.516901928666634	26.23853800324388	26.910868087338674	24.333691980750803
45	22.685252097016804	26.953424012247538	26.273599803011567	24.087724087724087
46	23.546808840926488	28.686975745799277	26.16118792589381	21.60502748738043
47	22.403151814916523	26.986076986076984	28.26596650126062	22.344804697745875
48	23.02543184896126	26.89400630577101	26.61618838089426	23.464373464373462
49	23.13757607875255	26.494141200023552	27.677142383024737	22.691140338199162
50	21.79693062046003	26.1649349884644	28.084501613913375	23.95363277716219
51	22.92024644965821	25.983470101117163	25.381263616557735	25.71501983266689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2904.0
1	2914.5
2	2925.0
3	2929.5
4	2934.0
5	2089.0
6	1244.0
7	1238.5
8	1233.0
9	1343.0
10	1453.0
11	1443.0
12	1433.0
13	1446.5
14	1460.0
15	1512.5
16	1565.0
17	1514.0
18	1463.0
19	1434.5
20	1406.0
21	1440.5
22	1475.0
23	1732.0
24	1989.0
25	2530.0
26	3680.0
27	4289.0
28	4568.5
29	4848.0
30	5646.5
31	6445.0
32	6750.0
33	7055.0
34	8136.5
35	9218.0
36	9950.5
37	10683.0
38	10749.5
39	10816.0
40	11883.5
41	12951.0
42	16221.5
43	19492.0
44	21226.0
45	22960.0
46	27411.0
47	31862.0
48	31955.5
49	32049.0
50	31855.5
51	31662.0
52	29224.5
53	26787.0
54	23885.0
55	20983.0
56	19467.5
57	17952.0
58	15995.5
59	14039.0
60	13673.5
61	13308.0
62	12362.0
63	11416.0
64	9979.0
65	8542.0
66	8050.5
67	7559.0
68	6447.5
69	5336.0
70	5276.0
71	5216.0
72	4726.5
73	4237.0
74	3600.5
75	2487.5
76	2011.0
77	1582.0
78	1153.0
79	948.0
80	743.0
81	531.5
82	320.0
83	217.0
84	114.0
85	71.5
86	29.0
87	20.5
88	12.0
89	9.0
90	6.0
91	6.5
92	7.0
93	6.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.407159861998746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16911078478388	20.327646761008676
2	9.821652529575067	5.383672019741366
3	3.5106991885840535	2.886548816667374
4	1.829576018975195	2.0057392972692973
5	1.1570262921995444	1.5855402277424298
6	0.8203049259695305	1.3489336944979182
7	0.6424552855877026	1.232551230140176
8	0.5435391268184294	1.1917490991971127
9	0.4725992754699919	1.1657343504123767
>10	6.00690664456632	39.16286876121567
>50	0.9252154944863386	16.21266201214461
>100	0.08992375216389899	4.185087636713218
>500	0.006994069612747699	1.5266546865586725
>1k	0.0039966112072843995	1.7846114066910972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2492	0.6669771375653728	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	1428	0.38220038220038216	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1379	0.36908566320331027	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.3259944436415025	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	958	0.2564061387590799	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	873	0.2336561160090572	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	859	0.2299090534384652	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	838	0.22428845958257726	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	816	0.21840021840021842	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	616	0.1648707531060472	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	615	0.16460310577957638	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	478	0.1279354220530691	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	472	0.12632953809424396	Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	446	0.11937070760600173	No Hit
GTGAATGGGGGTTCAGTTTCATCAACGCTCGATGGACCAGGAATCTGATGA	419	0.11214422979128863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1322177792766028	0.0
2	0.0	0.0	0.0	0.6980242274359921	0.0
3	0.0	0.0	0.0	0.9113391466332643	0.0
4	0.0	0.0	0.0	1.2450953627424215	0.0
5	0.0	0.0	0.0	2.310599369422899	0.0
6	0.0	0.0	0.0	2.876405817582288	0.0
7	0.0	0.0	0.0	3.4960093783623196	0.0
8	0.0	0.0	0.0	4.4172514760750055	0.0
9	0.0	0.0	0.0	4.79704303233715	0.0
10	0.0	0.0	0.0	6.056591350708998	0.0
11	0.0	0.0	0.0	7.2331689978748805	0.0
12	0.0	0.0	0.0	8.340693634811283	0.0
13	0.0	0.0	0.0	8.70282046752635	0.0
14	0.0	0.0	0.0	8.846011787188258	0.0
15	0.0	0.0	0.0	9.129717953247365	0.0
16	0.0	0.0	0.0	9.65029200323318	0.0
17	0.0	0.0	0.0	10.315395609513256	0.0
18	0.0	0.0	0.0	11.1295787766376	0.0
19	0.0	0.0	0.0	11.58939688351453	0.0
20	0.0	0.0	0.0	11.978288448876684	0.0
21	0.0	0.0	0.0	12.410003586474176	0.0
22	0.0	0.0	0.0	12.85858050563933	0.0
23	0.0	0.0	0.0	13.325892737657444	0.0
24	0.0	0.0	0.0	13.707022530551942	0.0
25	0.0	0.0	0.0	14.032481679540503	0.0
26	0.0	0.0	0.0	14.321005497476087	0.0
27	0.0	0.0	0.0	14.626123449652862	0.0
28	0.0	0.0	0.0	14.949709067356126	0.0
29	0.0	0.0	0.0	15.265532912591736	0.0
30	0.0	0.0	0.0	15.644253879547998	0.0
31	0.0	0.0	0.0	15.976404211698329	0.0
32	0.0	0.0	0.0	16.328092798681034	0.0
33	2.6764732647085586E-4	0.0	0.0	16.671216671216673	0.0
34	2.6764732647085586E-4	0.0	0.0	17.000155235449352	0.0
35	2.6764732647085586E-4	0.0	0.0	17.37673502379385	0.0
36	2.6764732647085586E-4	0.0	0.0	17.6930941636824	0.0
37	5.352946529417117E-4	0.0	0.0	18.022032727915082	0.0
38	5.352946529417117E-4	0.0	0.0	18.348294818883055	0.0
39	5.352946529417117E-4	0.0	0.0	18.646453940571586	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	90	0.0	45.000004	16
GACAGTC	20	7.027167E-4	45.0	38
AACGTAC	20	7.027167E-4	45.0	21
AACGTAA	20	7.027167E-4	45.0	23
CGGAACA	20	7.027167E-4	45.0	19
ACGCCTA	25	3.885735E-5	45.0	17
GTCGATG	35	1.2089913E-7	45.0	1
GTACATC	20	7.027167E-4	45.0	36
TAGCGCG	25	3.885735E-5	45.0	1
ACTCTAC	25	3.885735E-5	45.0	45
TTGTCGG	20	7.027167E-4	45.0	2
CGACCGA	20	7.027167E-4	45.0	11
AAGGTTC	25	3.885735E-5	45.0	5
ATAGACG	20	7.027167E-4	45.0	1
TGTGCGT	20	7.027167E-4	45.0	39
TCGATAG	20	7.027167E-4	45.0	1
CCGTCGA	20	7.027167E-4	45.0	20
CCGTCAA	25	3.885735E-5	45.0	13
AGTATCG	20	7.027167E-4	45.0	1
CGTTAGG	35	1.2089913E-7	45.0	2
>>END_MODULE