FastQCFastQC Report
Sat 18 Jun 2016
SRR3553617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291224
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19940.6846963162376727No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.4618438040820812No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10600.3639809905776996No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC8710.2990824932011098No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC8190.28122682196522264No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG7840.2692085817102986No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC6350.21804521605362195No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC5240.17993022553086285No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC5170.17752657747987802No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC4310.14799604428206467No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT3400.11674861961926215Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.11331483668928384No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3120.10713402741532291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.0246053E-445.01
CAGCGAA253.8836104E-545.02
TCGTTGC253.8836104E-545.016
CGAACTC253.8836104E-545.025
CTCGTTG253.8836104E-545.015
TCCGCAA207.0246053E-445.032
CTCAAGG351.2077544E-745.02
GATCGAG207.0246053E-445.01
CGTGCGG253.8836104E-545.02
TAGACAG207.0246053E-445.01
TTTCGAG207.0246053E-445.01
CGTGACG207.0246053E-445.02
GGCAATC253.8836104E-545.09
GGCGTCA253.8836104E-545.036
CGTTGCG253.8836104E-545.017
ATAGCCG207.0246053E-445.01
ATTAACG207.0246053E-445.01
TAACGTG207.0246053E-445.040
CGACACA207.0246053E-445.039
GCGTTAG351.2077544E-745.01