FastQCFastQC Report
Sat 18 Jun 2016
SRR3553612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522871
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39170.7491331513891573No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.37638346743269374No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18080.3457831855275967No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC13040.24939229752654096TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC11880.22720709314534562No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC10710.20483063700224338No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG9900.189339244287788No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC7640.14611634609683843No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT7630.14592509433493156Illumina PCR Primer Index 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC6320.12087111352513334TruSeq Adapter, Index 13 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.12010610647750593No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC5850.1118822807155111No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5360.10251094438207511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT207.029754E-445.00000433
TTTACGT207.029754E-445.00000434
CGAAAGG650.045.0000042
CAACGAT207.029754E-445.00000431
CGCATCG302.1632222E-645.00000421
GCCGCAT302.1632222E-645.00000419
CCCTTTA207.029754E-445.00000435
CGTACTT207.029754E-445.00000438
TTACGTA207.029754E-445.00000435
CATCGAA207.029754E-445.00000442
GCGTGTC207.029754E-445.0000049
CACGTCG207.029754E-445.0000042
CCAGTCG302.1632222E-645.00000428
GAATGCG351.2102282E-745.01
TTCGTAG351.2102282E-745.01
CGATTCG253.887879E-545.010
GAATAAC351.2102282E-745.09
ATCACGC351.2102282E-745.042
TATTACG253.887879E-545.01
CCGACGT253.887879E-545.024