Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553612_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 522871 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3917 | 0.7491331513891573 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1968 | 0.37638346743269374 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1808 | 0.3457831855275967 | No Hit |
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC | 1304 | 0.24939229752654096 | TruSeq Adapter, Index 13 (95% over 22bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC | 1188 | 0.22720709314534562 | No Hit |
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC | 1071 | 0.20483063700224338 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG | 990 | 0.189339244287788 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC | 764 | 0.14611634609683843 | No Hit |
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT | 763 | 0.14592509433493156 | Illumina PCR Primer Index 1 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC | 632 | 0.12087111352513334 | TruSeq Adapter, Index 13 (95% over 22bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 628 | 0.12010610647750593 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC | 585 | 0.1118822807155111 | No Hit |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 536 | 0.10251094438207511 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTAGACT | 20 | 7.029754E-4 | 45.000004 | 33 |
TTTACGT | 20 | 7.029754E-4 | 45.000004 | 34 |
CGAAAGG | 65 | 0.0 | 45.000004 | 2 |
CAACGAT | 20 | 7.029754E-4 | 45.000004 | 31 |
CGCATCG | 30 | 2.1632222E-6 | 45.000004 | 21 |
GCCGCAT | 30 | 2.1632222E-6 | 45.000004 | 19 |
CCCTTTA | 20 | 7.029754E-4 | 45.000004 | 35 |
CGTACTT | 20 | 7.029754E-4 | 45.000004 | 38 |
TTACGTA | 20 | 7.029754E-4 | 45.000004 | 35 |
CATCGAA | 20 | 7.029754E-4 | 45.000004 | 42 |
GCGTGTC | 20 | 7.029754E-4 | 45.000004 | 9 |
CACGTCG | 20 | 7.029754E-4 | 45.000004 | 2 |
CCAGTCG | 30 | 2.1632222E-6 | 45.000004 | 28 |
GAATGCG | 35 | 1.2102282E-7 | 45.0 | 1 |
TTCGTAG | 35 | 1.2102282E-7 | 45.0 | 1 |
CGATTCG | 25 | 3.887879E-5 | 45.0 | 10 |
GAATAAC | 35 | 1.2102282E-7 | 45.0 | 9 |
ATCACGC | 35 | 1.2102282E-7 | 45.0 | 42 |
TATTACG | 25 | 3.887879E-5 | 45.0 | 1 |
CCGACGT | 25 | 3.887879E-5 | 45.0 | 24 |