##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522871
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79988563144638	31.0	31.0	34.0	30.0	34.0
2	31.889955648716413	33.0	31.0	34.0	30.0	34.0
3	31.876875940719604	33.0	31.0	34.0	30.0	34.0
4	35.584201456955924	37.0	35.0	37.0	33.0	37.0
5	35.48402378406911	37.0	35.0	37.0	33.0	37.0
6	35.464363102945086	37.0	35.0	37.0	33.0	37.0
7	35.72334667633125	37.0	35.0	37.0	35.0	37.0
8	35.61878360054392	37.0	35.0	37.0	35.0	37.0
9	37.34969810909383	39.0	37.0	39.0	34.0	39.0
10	36.85888106244179	39.0	37.0	39.0	32.0	39.0
11	36.80807885692647	39.0	37.0	39.0	32.0	39.0
12	36.734664955600906	39.0	35.0	39.0	33.0	39.0
13	36.64676373331089	39.0	35.0	39.0	32.0	39.0
14	37.67648425711122	40.0	36.0	41.0	33.0	41.0
15	37.8457535415045	40.0	36.0	41.0	33.0	41.0
16	37.89602597963934	40.0	36.0	41.0	33.0	41.0
17	37.81190962971746	39.0	36.0	41.0	33.0	41.0
18	37.7179552891631	39.0	36.0	41.0	33.0	41.0
19	37.582604504744	39.0	36.0	41.0	33.0	41.0
20	37.469909404040386	39.0	35.0	41.0	33.0	41.0
21	37.33109313769553	39.0	35.0	41.0	32.0	41.0
22	37.29495611728323	39.0	35.0	41.0	32.0	41.0
23	37.21091053051326	39.0	35.0	41.0	32.0	41.0
24	37.15407433190978	39.0	35.0	41.0	32.0	41.0
25	37.02482256617789	39.0	35.0	41.0	32.0	41.0
26	36.996498180239485	39.0	35.0	41.0	32.0	41.0
27	36.97622166844212	39.0	35.0	41.0	32.0	41.0
28	36.85066297423265	39.0	35.0	41.0	31.0	41.0
29	36.77546278145088	39.0	35.0	41.0	31.0	41.0
30	36.60283894115375	39.0	35.0	40.0	31.0	41.0
31	36.47842010744524	39.0	35.0	40.0	31.0	41.0
32	36.28941172870555	38.0	35.0	40.0	30.0	41.0
33	36.1098951749093	38.0	35.0	40.0	30.0	41.0
34	35.9688776007849	38.0	35.0	40.0	30.0	41.0
35	35.865500285921385	38.0	35.0	40.0	29.0	41.0
36	35.68626869725038	38.0	35.0	40.0	28.0	41.0
37	35.5951219325608	38.0	35.0	40.0	27.0	41.0
38	35.48605487778056	38.0	35.0	40.0	27.0	41.0
39	35.34846453522953	38.0	35.0	40.0	26.0	41.0
40	35.20449020886605	38.0	34.0	40.0	26.0	41.0
41	35.044471772196204	38.0	34.0	40.0	25.0	41.0
42	34.902299037429884	38.0	34.0	40.0	24.0	41.0
43	34.76777828565746	38.0	34.0	40.0	24.0	41.0
44	34.6637048143806	37.0	34.0	40.0	24.0	41.0
45	34.60468643317377	37.0	34.0	40.0	23.0	41.0
46	34.43221176925093	37.0	33.0	40.0	23.0	41.0
47	34.298211222270886	37.0	33.0	40.0	23.0	41.0
48	34.229863580118234	37.0	33.0	40.0	23.0	41.0
49	34.13286833654955	36.0	33.0	40.0	23.0	41.0
50	33.970072924296815	36.0	33.0	40.0	22.0	41.0
51	32.371445346940256	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	3.0
12	4.0
13	9.0
14	14.0
15	27.0
16	88.0
17	175.0
18	355.0
19	678.0
20	1121.0
21	1687.0
22	2306.0
23	3332.0
24	4599.0
25	5726.0
26	6729.0
27	7512.0
28	8311.0
29	9583.0
30	11516.0
31	13852.0
32	17652.0
33	23389.0
34	38661.0
35	50513.0
36	45183.0
37	61895.0
38	93746.0
39	114175.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.430788856142335	19.897450805265542	22.8723719617267	13.799388376865421
2	29.85478253718412	27.039747853677103	25.381403826182748	17.724065782956025
3	29.889399106089265	28.95130921393613	25.84671936290213	15.31257231707247
4	26.10051045095253	28.24807648540462	26.554159630195596	19.097253433447257
5	22.956522736965717	32.98327885845648	25.2852041899436	18.7749942146342
6	22.037366769241363	38.25819370360949	25.82893294904479	13.875506578104352
7	76.1876256285011	9.970336851728247	9.387592733198055	4.454444786572596
8	77.08612640593951	8.781707151477134	8.795668530096332	5.336497912487019
9	72.09866295893251	11.602670639603268	10.299672385731853	5.99899401573237
10	38.487122062612	27.605661817159486	18.07616027662655	15.831055843601959
11	29.316408827416325	29.87199519575574	22.457164386626914	18.354431590201024
12	26.776776681055175	24.593446567126502	29.674049622182146	18.955727129636184
13	22.686475249153233	28.73462096769567	29.794538232183466	18.78436555096764
14	19.630080842119757	31.67473430348977	28.799072811458277	19.896112042932195
15	19.142197597495368	28.812269183029848	32.913280713598574	19.13225250587621
16	19.807371225407415	26.229796642001563	32.40684604806921	21.555986084521802
17	20.323942234317833	27.678911241969818	28.30143572697664	23.695710796735714
18	22.05190190314628	27.33006802825171	28.55121052802699	22.06681954057502
19	22.70254039715341	29.29403237127322	26.378399261003192	21.62502797057018
20	23.99769732878664	28.220918735213846	27.37520344406173	20.406180491937782
21	22.43364041991237	30.31110924109388	27.14818760267829	20.10706273631546
22	20.54426426403453	28.335669792357965	26.45336995167068	24.666695991936827
23	20.7330297530366	30.390096218761414	25.762186084139298	23.114687944062684
24	21.322850186757343	27.845682778352593	29.003520944936707	21.827946089953354
25	20.7135220733221	30.02901289228127	26.399054451289132	22.858410583107496
26	19.259052424020457	30.679268882764582	26.775629170483732	23.28604952273123
27	20.824448095228078	30.09212597371053	26.344165195621866	22.739260735439526
28	18.77996676044378	30.113737422806008	29.175647530652878	21.930648286097334
29	21.498801807711654	28.889534894840217	27.53757618992065	22.07408710752748
30	21.87442026809672	29.527550772561494	26.084062799428544	22.51396615991325
31	22.806963859154553	30.410751409047354	22.91119606939379	23.8710886624043
32	22.693169060819972	29.85822506889845	24.49227438507777	22.95633148520381
33	21.36511682613876	30.22332468237864	22.940648840727444	25.470909650755157
34	21.020481151182604	29.33228272365459	25.689319162852787	23.957916962310016
35	21.49841930418784	27.766695800685063	25.58202692442304	25.152857970704055
36	22.686475249153233	30.1414689282825	24.399517280552946	22.77253854201132
37	21.037120054468502	29.86549263585091	25.823960403235212	23.273426906445376
38	20.360471320842045	30.706617884717264	25.005594114035773	23.927316680404918
39	21.700189912999573	28.209443629499436	26.048872475237676	24.04149398226331
40	22.358860981006785	26.23209166314445	28.3450411286914	23.064006227157368
41	19.72551547131128	27.617519426397713	27.18701171034538	25.469953391945623
42	21.757756693333537	27.12561989477328	27.57506153525439	23.541561876638788
43	21.837508678048696	26.501947899195024	27.235972161393533	24.424571261362747
44	21.143838537612528	27.46757804506274	26.20799394114418	25.180589476180547
45	21.071545371611737	27.26810245739389	26.28277338004976	25.37757879094461
46	22.256541288386618	28.19892478259456	26.158650986572212	23.385882942446607
47	19.965345180742478	27.844726519543062	27.844344016019246	24.345584283695214
48	20.83592320094249	27.123516125392307	28.13332542826051	23.9072352454047
49	21.37066312723406	26.698363458673363	28.14040174345106	23.790571670641516
50	20.05752852998158	25.46134706265982	29.3156438203687	25.165480586989908
51	19.429457743879468	26.579404862767298	26.535416957528724	27.455720435824517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4476.0
1	4184.5
2	3893.0
3	4159.5
4	4426.0
5	3334.0
6	2242.0
7	2594.0
8	2946.0
9	3289.0
10	3632.0
11	3320.0
12	3008.0
13	3113.5
14	3219.0
15	3136.5
16	3054.0
17	3047.0
18	3040.0
19	3053.0
20	3066.0
21	2805.5
22	2545.0
23	2820.0
24	3095.0
25	3285.0
26	3856.5
27	4238.0
28	5804.5
29	7371.0
30	7971.5
31	8572.0
32	9514.0
33	10456.0
34	11416.5
35	12377.0
36	13365.5
37	14354.0
38	15887.0
39	17420.0
40	18789.0
41	20158.0
42	23842.0
43	27526.0
44	31188.0
45	34850.0
46	38996.5
47	43143.0
48	44242.5
49	45342.0
50	44841.0
51	44340.0
52	39403.0
53	34466.0
54	30328.0
55	26190.0
56	24874.5
57	23559.0
58	21886.5
59	20214.0
60	19474.0
61	18734.0
62	17501.5
63	16269.0
64	13887.5
65	11506.0
66	10214.0
67	8922.0
68	7916.0
69	6910.0
70	6448.0
71	5986.0
72	5338.5
73	4691.0
74	3839.0
75	2559.0
76	2131.0
77	1872.5
78	1614.0
79	1303.0
80	992.0
81	842.5
82	693.0
83	584.5
84	476.0
85	340.0
86	204.0
87	123.0
88	42.0
89	25.0
90	8.0
91	7.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.348829030937406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10437211714893	22.335742057730435
2	9.899292127458944	5.8106526435219426
3	3.4601708607382977	3.046558890289195
4	1.8342796977131735	2.153358449724139
5	1.087490677927473	1.5958288989615808
6	0.6914814752981475	1.217650295795142
7	0.5486655397309258	1.1271883784510954
8	0.4141769068931688	0.9724485783176072
9	0.356285964259812	0.9410918266065507
>10	4.4404946047383	31.74622165779032
>50	1.0290894339684327	20.289133472242664
>100	0.12569031254576277	5.66048417983763
>500	0.004582459311564268	0.9410444475203555
>1k	0.0039278222670550865	2.1625962232113354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3917	0.7491331513891573	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.37638346743269374	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1808	0.3457831855275967	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1304	0.24939229752654096	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	1188	0.22720709314534562	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1071	0.20483063700224338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	990	0.189339244287788	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	764	0.14611634609683843	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	763	0.14592509433493156	Illumina PCR Primer Index 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	632	0.12087111352513334	TruSeq Adapter, Index 13 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.12010610647750593	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	585	0.1118822807155111	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	536	0.10251094438207511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15606143771599496	0.0
2	0.0	0.0	0.0	0.7170028553888053	0.0
3	0.0	0.0	0.0	0.9554938024866554	0.0
4	0.0	0.0	0.0	1.331877269919349	0.0
5	0.0	0.0	0.0	2.440181230169583	0.0
6	1.9125176190685656E-4	0.0	0.0	3.1677029324632655	0.0
7	1.9125176190685656E-4	0.0	0.0	3.9195136085191185	0.0
8	1.9125176190685656E-4	0.0	0.0	5.1054657841035365	0.0
9	1.9125176190685656E-4	0.0	0.0	5.631599381109298	0.0
10	1.9125176190685656E-4	0.0	0.0	6.890992233265949	0.0
11	1.9125176190685656E-4	0.0	0.0	8.162816449946545	0.0
12	1.9125176190685656E-4	0.0	0.0	9.277240466577798	0.0
13	3.8250352381371313E-4	0.0	0.0	9.677912907772663	0.0
14	3.8250352381371313E-4	0.0	0.0	9.841050660679212	0.0
15	3.8250352381371313E-4	0.0	0.0	10.133665856396702	0.0
16	3.8250352381371313E-4	0.0	0.0	10.750644040308222	0.0
17	3.8250352381371313E-4	0.0	0.0	11.471854434458978	0.0
18	3.8250352381371313E-4	0.0	0.0	12.406310543135879	0.0
19	3.8250352381371313E-4	0.0	0.0	12.871243576331446	0.0
20	3.8250352381371313E-4	0.0	0.0	13.299647523002806	0.0
21	3.8250352381371313E-4	0.0	0.0	13.783896984150967	0.0
22	3.8250352381371313E-4	0.0	0.0	14.305631790632871	0.0
23	3.8250352381371313E-4	0.0	0.0	14.827366597114777	0.0
24	3.8250352381371313E-4	0.0	0.0	15.235115353500195	0.0
25	3.8250352381371313E-4	0.0	0.0	15.606335023361403	0.0
26	3.8250352381371313E-4	0.0	0.0	15.958047013508112	0.0
27	3.8250352381371313E-4	0.0	0.0	16.314922801226306	0.0
28	3.8250352381371313E-4	0.0	0.0	16.700677604992435	0.0
29	3.8250352381371313E-4	0.0	0.0	17.084711142901405	0.0
30	3.8250352381371313E-4	0.0	0.0	17.493033654572542	0.0
31	3.8250352381371313E-4	0.0	0.0	17.904989949719912	0.0
32	3.8250352381371313E-4	0.0	0.0	18.322301294200674	0.0
33	3.8250352381371313E-4	0.0	0.0	18.72641626710986	0.0
34	3.8250352381371313E-4	0.0	0.0	19.12976623297142	0.0
35	3.8250352381371313E-4	0.0	0.0	19.53808874464256	0.0
36	3.8250352381371313E-4	0.0	0.0	19.92326979312297	0.0
37	3.8250352381371313E-4	0.0	0.0	20.32967978717504	0.0
38	3.8250352381371313E-4	0.0	0.0	20.74469611051292	0.0
39	3.8250352381371313E-4	0.0	0.0	21.14479479642206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACT	20	7.029754E-4	45.000004	33
TTTACGT	20	7.029754E-4	45.000004	34
CGAAAGG	65	0.0	45.000004	2
CAACGAT	20	7.029754E-4	45.000004	31
CGCATCG	30	2.1632222E-6	45.000004	21
GCCGCAT	30	2.1632222E-6	45.000004	19
CCCTTTA	20	7.029754E-4	45.000004	35
CGTACTT	20	7.029754E-4	45.000004	38
TTACGTA	20	7.029754E-4	45.000004	35
CATCGAA	20	7.029754E-4	45.000004	42
GCGTGTC	20	7.029754E-4	45.000004	9
CACGTCG	20	7.029754E-4	45.000004	2
CCAGTCG	30	2.1632222E-6	45.000004	28
GAATGCG	35	1.2102282E-7	45.0	1
TTCGTAG	35	1.2102282E-7	45.0	1
CGATTCG	25	3.887879E-5	45.0	10
GAATAAC	35	1.2102282E-7	45.0	9
ATCACGC	35	1.2102282E-7	45.0	42
TATTACG	25	3.887879E-5	45.0	1
CCGACGT	25	3.887879E-5	45.0	24
>>END_MODULE