FastQCFastQC Report
Sat 18 Jun 2016
SRR3553610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261383
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG8540.32672362012831746No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.27316237092695395No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC6720.2570939961665449No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.25097271054353193No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC4680.17904760447312948No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4190.16030116725265225No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.12663409632608089No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT2750.10520959664553549No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG406.7811925E-945.0000042
GTGGAAC207.0232735E-445.00000435
CGTAGCC207.0232735E-445.00000439
GGTATGC207.0232735E-445.0000048
TCGTTCA253.8825106E-545.016
ACGGGTA351.2071177E-745.05
CGTTAGG253.8825106E-545.02
CGAATGC502.1827873E-1145.045
AGTTCGC253.8825106E-545.037
CGTCGAC253.8825106E-545.043
CTAGGGA1000.045.04
CGTATGG302.1590458E-644.9999962
GATGAAT3250.043.61538720
CGATGAA3300.042.27272819
TGAATGA3250.041.53846422
ATAGCGG603.6379788E-1241.2499962
TCAAGCG603.6379788E-1241.24999617
GTTGATC1100.040.90909216
TACACGC900.040.00000435
ATGAATG3500.039.85714321