##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261383
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.950635657253915	33.0	31.0	34.0	30.0	34.0
2	32.055466499351525	33.0	31.0	34.0	30.0	34.0
3	32.02743483700164	33.0	31.0	34.0	30.0	34.0
4	35.703488750224764	37.0	35.0	37.0	33.0	37.0
5	35.57929934234438	37.0	35.0	37.0	33.0	37.0
6	35.549546068413015	37.0	35.0	37.0	33.0	37.0
7	35.770681337347874	37.0	35.0	37.0	35.0	37.0
8	35.64907052103618	37.0	35.0	37.0	35.0	37.0
9	37.34271547881844	39.0	37.0	39.0	34.0	39.0
10	36.88616704223305	39.0	37.0	39.0	32.0	39.0
11	36.8085376631227	39.0	37.0	39.0	32.0	39.0
12	36.71811479706025	39.0	35.0	39.0	32.0	39.0
13	36.688300310272666	39.0	35.0	39.0	32.0	39.0
14	37.79650551106996	40.0	37.0	41.0	33.0	41.0
15	37.92172788589924	40.0	37.0	41.0	33.0	41.0
16	37.96325315724435	40.0	37.0	41.0	33.0	41.0
17	37.855231595015745	40.0	36.0	41.0	33.0	41.0
18	37.85983403664355	40.0	36.0	41.0	33.0	41.0
19	37.76804153292295	40.0	36.0	41.0	33.0	41.0
20	37.57765424683319	40.0	36.0	41.0	32.0	41.0
21	37.483987099390546	39.0	35.0	41.0	32.0	41.0
22	37.45073321524353	39.0	35.0	41.0	32.0	41.0
23	37.29500388319057	39.0	35.0	41.0	32.0	41.0
24	37.287126553754455	39.0	35.0	41.0	32.0	41.0
25	37.197162784113736	39.0	35.0	41.0	32.0	41.0
26	37.10054976796501	39.0	35.0	41.0	32.0	41.0
27	37.00647708534985	39.0	35.0	41.0	31.0	41.0
28	36.88393659878416	39.0	35.0	41.0	31.0	41.0
29	36.849997895808066	39.0	35.0	41.0	31.0	41.0
30	36.69187743655861	39.0	35.0	41.0	31.0	41.0
31	36.62727491841474	39.0	35.0	41.0	30.0	41.0
32	36.361056380866394	39.0	35.0	41.0	30.0	41.0
33	36.232210970108994	39.0	35.0	41.0	30.0	41.0
34	36.03656702999047	39.0	35.0	41.0	29.0	41.0
35	35.912603344517436	39.0	35.0	41.0	28.0	41.0
36	35.746858058863815	38.0	35.0	40.0	27.0	41.0
37	35.655532303171974	38.0	35.0	40.0	27.0	41.0
38	35.517424622106255	38.0	35.0	40.0	27.0	41.0
39	35.39860281655655	38.0	34.0	40.0	26.0	41.0
40	35.243393793781536	38.0	34.0	40.0	26.0	41.0
41	35.13203613088839	38.0	34.0	40.0	25.0	41.0
42	34.95717012965648	38.0	34.0	40.0	24.0	41.0
43	34.87144152450618	38.0	34.0	40.0	24.0	41.0
44	34.817646901290445	37.0	34.0	40.0	24.0	41.0
45	34.74929509570247	37.0	34.0	40.0	24.0	41.0
46	34.5781095174514	37.0	34.0	40.0	23.0	41.0
47	34.47423512623239	37.0	33.0	40.0	23.0	41.0
48	34.409536963000654	37.0	33.0	40.0	23.0	41.0
49	34.267071691732056	36.0	33.0	40.0	23.0	41.0
50	34.08808912591867	36.0	33.0	40.0	23.0	41.0
51	32.59742217359201	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	6.0
13	7.0
14	15.0
15	23.0
16	46.0
17	104.0
18	173.0
19	328.0
20	625.0
21	1102.0
22	1567.0
23	1882.0
24	2313.0
25	2762.0
26	3027.0
27	3418.0
28	3964.0
29	4509.0
30	5516.0
31	6536.0
32	8503.0
33	11371.0
34	18298.0
35	22025.0
36	22498.0
37	30957.0
38	47457.0
39	62334.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.700370720360546	17.794194725747275	22.350344131026116	14.155090422866062
2	30.213900674489157	26.76761686873286	25.466461093491162	17.552021363286823
3	31.823416212990136	26.28709594732634	25.834120811223375	16.05536702846015
4	27.672036819533023	26.167730877677585	27.92989597640244	18.23033632638695
5	26.84336777831764	30.050921444776442	23.775455940133828	19.33025483677209
6	23.69587922703466	38.445882096387294	23.80453204684314	14.053706629734911
7	80.78604959006516	6.669140686272634	9.119950417586454	3.4248593060757586
8	83.23762448208186	7.881155239629203	5.791501360073149	3.089718918215798
9	77.93161758798391	7.465672977967197	7.337508560235364	7.265200873813522
10	43.955039157098966	24.493176679432096	15.626877034849244	15.924907128619688
11	33.02663141826362	27.038101177199742	20.537295845559964	19.397971558976675
12	27.09778371202412	24.199737549878915	27.22747845116171	21.475000286935263
13	26.11378704812478	24.762130666493228	28.731019232314264	20.39306305306772
14	22.62848004652177	28.545850342218127	27.691930997807813	21.13373861345229
15	22.266941614412566	25.401039853395208	29.361129071133163	22.97088946105906
16	24.355447752914305	23.874544251156347	27.719094202759937	24.05091379316941
17	24.18213885371275	25.849041444929473	26.40033973135208	23.5684799700057
18	25.43508950467322	24.28581812895253	26.111491566016156	24.167600800358095
19	25.771377633587495	25.490563655631775	24.453388322882514	24.28467038789822
20	27.84266765627451	24.96336793134978	25.53264749428999	21.66131691808572
21	26.894250965058937	26.855992929915107	25.665402876239078	20.584353228786874
22	25.309985729752892	23.465948435820234	26.57900475547377	24.645061078953105
23	26.187625055952374	25.743066687581056	24.90712861968835	23.162179636778216
24	23.11856547671425	27.114617247487406	26.653225343652803	23.11359193214555
25	24.55362437495935	27.388927359468674	25.204776133107355	22.852672132464622
26	23.497702604989612	25.525378467612658	26.77565105611306	24.201267871284667
27	25.993656817773154	25.220844507867763	25.619493234066486	23.166005440292597
28	23.332045312816824	26.928300616336948	27.619240731034534	22.120413339811694
29	24.273958138057946	25.450010138379316	26.931743839499887	23.34428788406285
30	24.253298799080277	27.508675009468863	26.081650298603964	22.156375892846896
31	26.070555468412255	25.885386578316112	23.970189339015928	24.07386861425571
32	27.048430846688575	26.626827299403555	23.603294781986587	22.72144707192128
33	25.753396357069892	26.000160683747602	23.811801073520467	24.434641885662035
34	23.47742584636338	26.671206620170402	24.956481485023893	24.894886048442324
35	26.09159738774136	26.555667354036032	24.31106843214746	23.041666826075147
36	24.992444038059094	28.585256118416268	24.281609745086712	22.140690098437922
37	25.404483076558154	27.284482923526017	24.416660609144436	22.894373390771396
38	24.420486412658818	28.144140973207897	24.427372858984707	23.007999755148575
39	24.881495736141986	26.516644158189322	24.484377331349016	24.117482774319676
40	23.834373314255327	26.6761801647391	26.339892035824825	23.14955448518075
41	22.72603803613854	26.1688786187319	25.598451314737382	25.506632030392183
42	23.35040916968586	26.647486638381224	25.50433654828355	24.497767643649357
43	24.2884961914126	24.61292432943229	26.163905074163203	24.93467440499191
44	24.61713271329811	24.654243007387628	26.69186595914807	24.036758320166193
45	23.97669320499038	24.912484744608488	26.229326314259154	24.881495736141986
46	25.149301982148803	26.563318961064798	24.65271268598187	23.634666370804528
47	23.633518629750213	27.440193126561407	26.41946874892399	22.50681949476439
48	23.509180015532763	27.10428757799857	25.774438276399003	23.612094130069668
49	24.40212255578978	26.603872478317257	26.422911972086936	22.571092993806026
50	23.945704196523877	25.713990580871748	27.02432828454796	23.315976938056416
51	23.373363990772162	26.20331085036135	25.47105205770842	24.95227310115807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	799.0
1	898.0
2	997.0
3	1258.0
4	1519.0
5	1133.0
6	747.0
7	743.5
8	740.0
9	770.5
10	801.0
11	834.5
12	868.0
13	863.0
14	858.0
15	823.5
16	789.0
17	739.0
18	689.0
19	687.5
20	686.0
21	694.0
22	702.0
23	785.0
24	868.0
25	1115.5
26	1757.5
27	2152.0
28	2561.5
29	2971.0
30	3318.0
31	3665.0
32	3926.5
33	4188.0
34	4913.0
35	5638.0
36	6409.0
37	7180.0
38	7716.0
39	8252.0
40	9124.0
41	9996.0
42	11186.5
43	12377.0
44	14623.0
45	16869.0
46	16446.0
47	16023.0
48	17174.0
49	18325.0
50	17603.0
51	16881.0
52	16664.0
53	16447.0
54	16034.5
55	15622.0
56	15282.5
57	14943.0
58	14508.0
59	14073.0
60	13473.5
61	12874.0
62	12068.5
63	11263.0
64	10255.0
65	9247.0
66	8435.0
67	7623.0
68	7058.5
69	6494.0
70	5646.5
71	4799.0
72	4264.5
73	3730.0
74	3197.0
75	2388.0
76	2112.0
77	1618.0
78	1124.0
79	884.0
80	644.0
81	499.5
82	355.0
83	288.5
84	222.0
85	165.0
86	108.0
87	82.5
88	57.0
89	39.5
90	22.0
91	15.0
92	8.0
93	7.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.863204569539715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81569310199916	22.24551711473202
2	7.694062556282644	4.903149784033391
3	3.8482319745452362	3.6785100790793583
4	2.5838986612235098	3.2932516651809793
5	1.9139100678393468	3.0491654009633375
6	1.656961037401693	3.1677653099092136
7	1.3832022573092395	3.0851279539985383
8	1.173080386624242	2.9902480268418374
9	1.0590142282523864	3.0369228297173114
>10	8.798703247883772	47.04934903953203
>50	0.04562646334874227	0.9981521369025529
>100	0.022813231674371135	1.3948879613440812
>500	0.004802785615657081	1.1079526977653482
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	854	0.32672362012831746	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.27316237092695395	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	672	0.2570939961665449	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	656	0.25097271054353193	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	468	0.17904760447312948	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	419	0.16030116725265225	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	331	0.12663409632608089	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	275	0.10520959664553549	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8258035143831084E-4	0.0	0.0	0.020276758626230473	0.0
2	3.8258035143831084E-4	0.0	0.0	0.18210824728463595	0.0
3	3.8258035143831084E-4	0.0	0.0	0.24255594281188908	0.0
4	3.8258035143831084E-4	0.0	0.0	0.3711029408951615	0.0
5	3.8258035143831084E-4	0.0	0.0	0.8458851570301053	0.0
6	3.8258035143831084E-4	0.0	0.0	1.117517206551306	0.0
7	3.8258035143831084E-4	0.0	0.0	1.3359705872225813	0.0
8	3.8258035143831084E-4	0.0	0.0	1.7017174031976066	0.0
9	3.8258035143831084E-4	0.0	0.0	1.858575347287314	0.0
10	3.8258035143831084E-4	0.0	0.0	2.4779729362659393	0.0
11	3.8258035143831084E-4	0.0	0.0	2.790541083391039	0.0
12	3.8258035143831084E-4	0.0	0.0	3.263027817417353	0.0
13	3.8258035143831084E-4	0.0	0.0	3.3927225565549404	0.0
14	3.8258035143831084E-4	0.0	0.0	3.4623521805167132	0.0
15	3.8258035143831084E-4	0.0	0.0	3.57177016102807	0.0
16	3.8258035143831084E-4	0.0	0.0	3.7336016496864755	0.0
17	3.8258035143831084E-4	0.0	0.0	3.9317782717315204	0.0
18	3.8258035143831084E-4	0.0	0.0	4.152144554159987	0.0
19	3.8258035143831084E-4	0.0	0.0	4.344199890582019	0.0
20	3.8258035143831084E-4	0.0	0.0	4.489580424128578	0.0
21	3.8258035143831084E-4	0.0	0.0	4.6330480559179446	0.0
22	3.8258035143831084E-4	0.0	0.0	4.79028858035909	0.0
23	3.8258035143831084E-4	0.0	0.0	4.942172979880099	0.0
24	3.8258035143831084E-4	0.0	0.0	5.0684244958547415	0.0
25	3.8258035143831084E-4	0.0	0.0	5.182050860231921	0.0
26	3.8258035143831084E-4	0.0	0.0	5.3132759207752605	0.0
27	3.8258035143831084E-4	0.0	0.0	5.44258807956141	0.0
28	3.8258035143831084E-4	0.0	0.0	5.558509926047218	0.0
29	3.8258035143831084E-4	0.0	0.0	5.695473691862134	0.0
30	3.8258035143831084E-4	0.0	0.0	5.865339367900743	0.0
31	3.8258035143831084E-4	0.0	0.0	6.000390231958467	0.0
32	3.8258035143831084E-4	0.0	0.0	6.133528194258999	0.0
33	3.8258035143831084E-4	0.0	0.0	6.273170022533983	0.0
34	3.8258035143831084E-4	0.0	0.0	6.404395083077324	0.0
35	3.8258035143831084E-4	0.0	0.0	6.554366580841141	0.0
36	3.8258035143831084E-4	0.0	0.0	6.6875045431416735	0.0
37	3.8258035143831084E-4	0.0	0.0	6.8271463714166565	0.0
38	3.8258035143831084E-4	0.0	0.0	6.966405619340202	0.0
39	3.8258035143831084E-4	0.0	0.0	7.106047447615185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	40	6.7811925E-9	45.000004	2
GTGGAAC	20	7.0232735E-4	45.000004	35
CGTAGCC	20	7.0232735E-4	45.000004	39
GGTATGC	20	7.0232735E-4	45.000004	8
TCGTTCA	25	3.8825106E-5	45.0	16
ACGGGTA	35	1.2071177E-7	45.0	5
CGTTAGG	25	3.8825106E-5	45.0	2
CGAATGC	50	2.1827873E-11	45.0	45
AGTTCGC	25	3.8825106E-5	45.0	37
CGTCGAC	25	3.8825106E-5	45.0	43
CTAGGGA	100	0.0	45.0	4
CGTATGG	30	2.1590458E-6	44.999996	2
GATGAAT	325	0.0	43.615387	20
CGATGAA	330	0.0	42.272728	19
TGAATGA	325	0.0	41.538464	22
ATAGCGG	60	3.6379788E-12	41.249996	2
TCAAGCG	60	3.6379788E-12	41.249996	17
GTTGATC	110	0.0	40.909092	16
TACACGC	90	0.0	40.000004	35
ATGAATG	350	0.0	39.857143	21
>>END_MODULE