##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544509
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.991586915918745	33.0	31.0	34.0	30.0	34.0
2	32.083302571674665	33.0	31.0	34.0	30.0	34.0
3	32.06355450506787	33.0	31.0	34.0	30.0	34.0
4	35.72235720621698	37.0	35.0	37.0	33.0	37.0
5	35.63199139040861	37.0	35.0	37.0	33.0	37.0
6	35.5941371033353	37.0	35.0	37.0	33.0	37.0
7	35.858823270138785	37.0	35.0	37.0	35.0	37.0
8	35.76961262348281	37.0	35.0	37.0	35.0	37.0
9	37.48631886708943	39.0	37.0	39.0	35.0	39.0
10	36.962973982064575	39.0	37.0	39.0	33.0	39.0
11	36.896934669583054	39.0	37.0	39.0	33.0	39.0
12	36.77031968250295	39.0	35.0	39.0	33.0	39.0
13	36.74014938228753	39.0	35.0	39.0	33.0	39.0
14	37.79119904354198	40.0	37.0	41.0	33.0	41.0
15	37.95397872211478	40.0	37.0	41.0	33.0	41.0
16	37.98765309664303	40.0	37.0	41.0	33.0	41.0
17	37.931598926739504	40.0	36.0	41.0	33.0	41.0
18	37.87865398000768	40.0	36.0	41.0	33.0	41.0
19	37.80598300487228	40.0	36.0	41.0	33.0	41.0
20	37.73258660554738	40.0	36.0	41.0	33.0	41.0
21	37.58586359454114	40.0	35.0	41.0	33.0	41.0
22	37.571869335493076	39.0	35.0	41.0	33.0	41.0
23	37.517455175212895	39.0	35.0	41.0	33.0	41.0
24	37.46909601126887	39.0	35.0	41.0	33.0	41.0
25	37.331889831022075	39.0	35.0	41.0	32.0	41.0
26	37.285512268851384	39.0	35.0	41.0	32.0	41.0
27	37.24457263332654	39.0	35.0	41.0	32.0	41.0
28	37.14405455189905	39.0	35.0	41.0	32.0	41.0
29	37.082689175018224	39.0	35.0	41.0	32.0	41.0
30	36.91370757875444	39.0	35.0	41.0	31.0	41.0
31	36.83791636134573	39.0	35.0	41.0	31.0	41.0
32	36.70246772780615	39.0	35.0	41.0	31.0	41.0
33	36.58469924280407	39.0	35.0	41.0	31.0	41.0
34	36.477553171756576	39.0	35.0	41.0	30.0	41.0
35	36.327849493764106	39.0	35.0	41.0	30.0	41.0
36	36.146811163819144	39.0	35.0	41.0	30.0	41.0
37	36.10222420566051	39.0	35.0	41.0	29.0	41.0
38	36.01155169152393	38.0	35.0	40.0	29.0	41.0
39	35.89567298244841	38.0	35.0	40.0	29.0	41.0
40	35.76180742650718	38.0	35.0	40.0	28.0	41.0
41	35.597240816956194	38.0	35.0	40.0	28.0	41.0
42	35.45775368267559	38.0	34.0	40.0	27.0	41.0
43	35.39221573931744	38.0	34.0	40.0	27.0	41.0
44	35.26761357479858	38.0	34.0	40.0	27.0	41.0
45	35.19164421524713	38.0	34.0	40.0	26.0	41.0
46	35.03976242816923	37.0	34.0	40.0	26.0	41.0
47	34.93018113566534	37.0	34.0	40.0	26.0	41.0
48	34.79660758591685	37.0	34.0	40.0	26.0	41.0
49	34.728944792464404	37.0	34.0	40.0	26.0	41.0
50	34.59476886516109	37.0	34.0	40.0	25.0	41.0
51	33.12082812221653	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	5.0
12	13.0
13	10.0
14	27.0
15	43.0
16	88.0
17	177.0
18	354.0
19	524.0
20	874.0
21	1225.0
22	1803.0
23	2535.0
24	3450.0
25	4452.0
26	5759.0
27	6525.0
28	7753.0
29	9264.0
30	11012.0
31	13604.0
32	17405.0
33	23684.0
34	38801.0
35	47565.0
36	46955.0
37	64553.0
38	101121.0
39	134882.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83615697812157	18.844316622865737	21.06833863168469	14.251187767327997
2	32.10672367215234	25.23410999634533	24.399964004268064	18.259202327234263
3	32.87971365027943	25.006198244657114	25.416476128034617	16.697611977028846
4	28.176577430308775	27.51781880556611	24.638343902488298	19.66725986163681
5	24.472873726605073	32.11553895344246	24.014479099519015	19.397108220433452
6	24.94687874764237	37.336389297513904	23.471788345096225	14.244943609747498
7	82.28146825855954	7.000067951126611	6.911731486531902	3.8067323037819394
8	84.26490654883574	5.771070817929547	6.528266750411839	3.4357558828228734
9	79.10888525258535	7.88728928263812	8.39214778819083	4.611677676585694
10	42.25623451586659	25.83152895544426	16.40193275042286	15.510303778266291
11	32.95464354124542	27.20505997146053	21.354100666839297	18.48619582045476
12	27.774012918060127	25.06625234844603	27.255380535491607	19.904354198002235
13	25.761924963591053	25.797553392138607	28.410366036190403	20.030155608079937
14	22.66151707317969	28.82578616698714	27.61441959637031	20.898277163462865
15	22.481354761812934	26.51856994099271	30.498485791786727	20.501589505407626
16	25.02842009957595	24.584533956279877	29.46617962237539	20.920866321768784
17	24.989669592238144	23.932386792504808	27.043079177754635	24.03486443750241
18	25.53658433561245	25.142100497879742	27.813498032172102	21.507817134335706
19	26.695242870182128	25.975328231489286	25.61720742907831	21.712221469250277
20	26.495246175912612	26.183038296887656	25.662202093996612	21.659513433203124
21	26.205994758580665	26.295984088417274	26.65135011542509	20.84667103757697
22	25.335302079488127	24.548905527732323	26.588173932845923	23.527618459933628
23	23.710902850090633	27.212956994282923	26.128126440517974	22.948013715108473
24	22.99980349268791	26.839776753001328	27.966663544587878	22.19375620972289
25	24.61869317127908	26.58945949470073	26.550708987362924	22.241138346657262
26	22.977214334381983	27.65114993507913	26.00856918802077	23.363066542518123
27	23.320275697922348	26.927194959128315	26.641432923973706	23.111096418975627
28	22.276215820124186	27.666576677336828	28.30513361578964	21.752073886749347
29	23.935876174682143	27.323698965490014	26.855203495259033	21.885221364568814
30	23.862599148958054	26.860529394371813	26.70516006163351	22.57171139503663
31	25.320058988923964	27.417912284278128	25.046234313849723	22.215794412948178
32	25.319875337230425	27.86528780975154	24.91455604957861	21.90028080343943
33	24.466262265637482	27.778604210398726	23.765447403073228	23.989686120890564
34	23.50337643638581	28.256282265306908	25.690117151415308	22.55022414689197
35	23.61062902541556	27.877041518138356	25.193890275459175	23.318439180986907
36	24.55735350563535	28.966830667629	24.358091418140013	22.117724408595635
37	23.276015639778223	29.575636031727665	24.718232389179974	22.430115939314135
38	22.994293941881587	29.58610417825968	24.505196424668828	22.914405455189904
39	23.83009279920075	26.949600465740698	25.887726373668755	23.3325803613898
40	24.305750685479946	27.16318738533247	26.300208077368787	22.230853851818793
41	22.33920835100981	27.693757127981357	26.222890714386722	23.744143806622112
42	23.04608371946102	28.16923136256701	25.5817626522243	23.202922265747674
43	22.74048730140365	26.917094115983392	26.26953824454692	24.07288033806604
44	23.03341175260648	26.728300175020063	26.46586190494556	23.772426167427906
45	23.702087568800515	27.36134756266655	25.112716226912685	23.82384864162025
46	23.596304193319117	27.503493973469677	25.733091647704633	23.167110185506576
47	23.022025347606743	28.068406582811306	26.336754764384057	22.572813305197894
48	22.975010514059456	27.897794159508845	26.08955958487371	23.03763574155799
49	23.020372482364845	27.363551382989076	26.82747208953387	22.7886040451122
50	22.607890778664817	27.265114075249446	26.92425653203161	23.20273861405413
51	22.691268647533832	27.281642727668416	25.886991766894578	24.140096857903178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1842.0
1	2191.5
2	2541.0
3	2559.5
4	2578.0
5	1919.0
6	1260.0
7	1276.0
8	1292.0
9	1353.5
10	1415.0
11	1428.0
12	1441.0
13	1490.5
14	1540.0
15	1718.5
16	1897.0
17	1839.0
18	1781.0
19	1844.5
20	1908.0
21	1814.5
22	1721.0
23	2140.0
24	2559.0
25	2989.0
26	3893.0
27	4367.0
28	5652.0
29	6937.0
30	8074.0
31	9211.0
32	10172.0
33	11133.0
34	12587.0
35	14041.0
36	15089.5
37	16138.0
38	17467.5
39	18797.0
40	21166.5
41	23536.0
42	26283.5
43	29031.0
44	31336.0
45	33641.0
46	36092.0
47	38543.0
48	39237.0
49	39931.0
50	38593.0
51	37255.0
52	34817.5
53	32380.0
54	32137.5
55	31895.0
56	30366.5
57	28838.0
58	28224.5
59	27611.0
60	25724.0
61	23837.0
62	21934.5
63	20032.0
64	19207.0
65	18382.0
66	16037.0
67	13692.0
68	12373.0
69	11054.0
70	9922.5
71	8791.0
72	7673.0
73	6555.0
74	5704.5
75	3893.0
76	2932.0
77	2221.5
78	1511.0
79	1350.5
80	1190.0
81	884.5
82	579.0
83	432.5
84	286.0
85	221.0
86	156.0
87	121.0
88	86.0
89	61.0
90	36.0
91	33.0
92	30.0
93	22.0
94	14.0
95	10.0
96	6.0
97	6.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.745475888400843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09946380675551	18.583719708619
2	7.619646120789879	3.771035387203059
3	2.8236100012620535	2.096147196134328
4	1.6170355724403451	1.6005725907403605
5	1.1310100930597773	1.3993691493674356
6	0.8883273429629736	1.3189249707778468
7	0.6837230058763921	1.1843335809381446
8	0.5965106418494073	1.1808751764047214
9	0.5458837583144538	1.2157338041315955
>10	7.855475652526202	47.75730673634007
>50	1.0842818655807354	16.72635701326658
>100	0.05205742838864984	2.4026506162899346
>500	0.001487355096818567	0.28248075183862204
>1k	0.001487355096818567	0.4804933179483011
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1568	0.28796585547713627	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.191548716366488	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	897	0.16473556910905054	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	638	0.11716978048113069	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.83651693544092E-4	0.0	0.0	0.04719848524083165	0.0
2	1.83651693544092E-4	0.0	0.0	0.243522145639466	0.0
3	1.83651693544092E-4	0.0	0.0	0.3223087221698815	0.0
4	1.83651693544092E-4	0.0	0.0	0.4347035586188658	0.0
5	1.83651693544092E-4	0.0	0.0	0.7904368890137721	0.0
6	1.83651693544092E-4	0.0	0.0	1.076015272474835	0.0
7	1.83651693544092E-4	0.0	0.0	1.3235777553722712	0.0
8	1.83651693544092E-4	0.0	0.0	1.7617706961684747	0.0
9	1.83651693544092E-4	0.0	0.0	1.9647058175346963	0.0
10	1.83651693544092E-4	0.0	0.0	2.3944507804278716	0.0
11	1.83651693544092E-4	0.0	0.0	2.9351213662216784	0.0
12	1.83651693544092E-4	0.0	0.0	3.3292379005673003	0.0
13	1.83651693544092E-4	0.0	0.0	3.4879129637893955	0.0
14	1.83651693544092E-4	0.0	0.0	3.55274201161046	0.0
15	1.83651693544092E-4	0.0	0.0	3.6717483090270315	0.0
16	1.83651693544092E-4	0.0	0.0	3.9391451748272295	0.0
17	1.83651693544092E-4	0.0	0.0	4.2384974353041	0.0
18	1.83651693544092E-4	0.0	0.0	4.605433519005196	0.0
19	1.83651693544092E-4	0.0	0.0	4.782106448194612	0.0
20	1.83651693544092E-4	0.0	0.0	4.955657298593779	0.0
21	1.83651693544092E-4	0.0	0.0	5.195506410362363	0.0
22	1.83651693544092E-4	0.0	0.0	5.447476533904857	0.0
23	3.67303387088184E-4	0.0	0.0	5.69981396083444	0.0
24	3.67303387088184E-4	0.0	0.0	5.900177958491044	0.0
25	3.67303387088184E-4	0.0	0.0	6.090992068083356	0.0
26	3.67303387088184E-4	0.0	0.0	6.271888986224287	0.0
27	3.67303387088184E-4	0.0	0.0	6.477027927913038	0.0
28	3.67303387088184E-4	0.0	0.0	6.6792284425050825	0.0
29	3.67303387088184E-4	0.0	0.0	6.884918339274465	0.0
30	3.67303387088184E-4	0.0	0.0	7.0968523936243475	0.0
31	3.67303387088184E-4	0.0	0.0	7.303460548861452	0.0
32	3.67303387088184E-4	0.0	0.0	7.5109869625662755	0.0
33	3.67303387088184E-4	0.0	0.0	7.709881746674527	0.0
34	3.67303387088184E-4	0.0	0.0	7.9309983857016135	0.0
35	3.67303387088184E-4	0.0	0.0	8.150645811180349	0.0
36	3.67303387088184E-4	0.0	0.0	8.36845671972364	0.0
37	3.67303387088184E-4	0.0	0.0	8.574330268186568	0.0
38	3.67303387088184E-4	0.0	0.0	8.797099772455551	0.0
39	3.67303387088184E-4	0.0	0.0	9.063027424707396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGA	20	7.030011E-4	45.000004	24
TACGAAT	75	0.0	45.000004	12
ACGGATC	20	7.030011E-4	45.000004	19
CGTACTA	20	7.030011E-4	45.000004	32
ATAACGG	40	6.8030204E-9	45.000004	2
ATGCCCG	20	7.030011E-4	45.000004	1
TAGTCCG	20	7.030011E-4	45.000004	13
GTTATGC	20	7.030011E-4	45.000004	38
CGTCTAC	25	3.8880928E-5	45.0	10
ACGGGTA	90	0.0	45.0	5
TATAGCG	25	3.8880928E-5	45.0	1
TATACGG	45	3.8380676E-10	45.0	2
GTTACGG	65	0.0	41.538464	2
GCGATTG	120	0.0	41.249996	9
TTACGGG	120	0.0	41.249996	3
TTGCGCA	50	1.0786607E-9	40.5	1
TTTACGG	45	1.9250365E-8	40.0	2
CGACAGG	90	0.0	40.0	2
GGCGATA	170	0.0	39.705883	8
ATGACGG	40	3.4537516E-7	39.375004	2
>>END_MODULE