FastQCFastQC Report
Sat 18 Jun 2016
SRR3553606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579175
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352232.2304684407997843No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG253.8914703E-545.0000041
ACTCGAC253.8914703E-545.00000428
TAACGCG700.045.01
CGTTTTT303450.044.028671
CTCGACG501.0822987E-940.5000041
TCGCACG451.9290383E-840.01
GCTACGA1200.039.37500410
GTTTTTT343500.038.94762
TCACGAC356.2497747E-638.57142625
ACGGGTA2800.038.5714265
TTGCGAG659.094947E-1238.076921
TCGATAG301.1401149E-437.5000041
CGGTCTA301.1401149E-437.50000431
TCGGCGT1100.036.8181844
CGTAAGG2700.036.6666682
TGGGCGA6950.036.258996
CGATCGC250.002107346436.00000410
AGGGCGA15800.035.6012656
CGCGACC1850.035.2702710
CTACGAA1350.035.00000411
Produced by FastQC (version 0.11.5)