##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1579175
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930770180632294	33.0	31.0	34.0	30.0	34.0
2	32.1234138078427	33.0	31.0	34.0	30.0	34.0
3	31.931562366425506	33.0	31.0	34.0	30.0	34.0
4	35.70665885668149	37.0	35.0	37.0	33.0	37.0
5	35.658023968211246	37.0	35.0	37.0	33.0	37.0
6	35.64391723526525	37.0	35.0	37.0	33.0	37.0
7	35.96887425396172	37.0	35.0	37.0	35.0	37.0
8	35.91810154036126	37.0	35.0	37.0	35.0	37.0
9	37.66654044041984	39.0	38.0	39.0	35.0	39.0
10	37.1409033197714	39.0	37.0	39.0	34.0	39.0
11	37.12430794560451	39.0	37.0	39.0	34.0	39.0
12	37.157979324647364	39.0	37.0	39.0	34.0	39.0
13	37.22667532097456	39.0	37.0	39.0	34.0	39.0
14	38.44101635347571	40.0	38.0	41.0	34.0	41.0
15	38.5355359602324	40.0	38.0	41.0	34.0	41.0
16	38.523038295312425	40.0	38.0	41.0	34.0	41.0
17	38.46036379755252	40.0	38.0	41.0	34.0	41.0
18	38.144575490366805	39.0	38.0	41.0	34.0	41.0
19	37.77263381195878	39.0	37.0	41.0	34.0	41.0
20	37.41479760001266	39.0	35.0	41.0	33.0	41.0
21	37.27947060965378	39.0	35.0	41.0	33.0	41.0
22	37.27244985514589	39.0	35.0	41.0	33.0	41.0
23	37.221143318504915	39.0	35.0	41.0	33.0	41.0
24	37.13044976015958	39.0	35.0	41.0	33.0	41.0
25	36.99838903224785	39.0	35.0	41.0	33.0	41.0
26	36.95733595073377	39.0	35.0	41.0	33.0	41.0
27	36.90787721436826	39.0	35.0	41.0	33.0	41.0
28	36.75042094764672	38.0	35.0	41.0	32.0	41.0
29	36.592301676508306	38.0	35.0	41.0	31.0	41.0
30	36.2032466319439	38.0	35.0	41.0	30.0	41.0
31	35.74634096917694	38.0	35.0	41.0	27.0	41.0
32	35.05941900042744	38.0	35.0	40.0	22.0	41.0
33	34.3630192980512	38.0	34.0	40.0	18.0	41.0
34	33.81703231117514	38.0	33.0	40.0	15.0	41.0
35	33.47981952601833	38.0	33.0	40.0	10.0	41.0
36	33.233677711463265	38.0	33.0	40.0	10.0	41.0
37	33.145571580097204	38.0	33.0	40.0	10.0	41.0
38	33.033799927177164	38.0	32.0	40.0	10.0	41.0
39	32.891010812607846	38.0	32.0	40.0	10.0	41.0
40	32.792927319644754	38.0	31.0	40.0	10.0	41.0
41	32.63950733769215	38.0	31.0	40.0	10.0	41.0
42	32.485122928111196	38.0	31.0	40.0	9.0	41.0
43	32.39709341903209	38.0	31.0	40.0	8.0	41.0
44	32.30431649437206	38.0	31.0	40.0	8.0	41.0
45	32.26308547184448	37.0	30.0	40.0	8.0	41.0
46	32.123655706302344	37.0	30.0	40.0	8.0	41.0
47	32.05271106748777	37.0	30.0	40.0	8.0	41.0
48	31.9519489606915	37.0	30.0	40.0	8.0	41.0
49	31.870247439327496	37.0	30.0	40.0	8.0	41.0
50	31.758284547311096	37.0	29.0	40.0	8.0	41.0
51	30.561899726122817	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	10.0
11	8.0
12	11.0
13	27.0
14	59.0
15	116.0
16	282.0
17	673.0
18	1224.0
19	2386.0
20	3959.0
21	6278.0
22	9391.0
23	15120.0
24	26055.0
25	44564.0
26	59163.0
27	57168.0
28	46948.0
29	39193.0
30	36084.0
31	37509.0
32	42683.0
33	53531.0
34	79381.0
35	98299.0
36	111172.0
37	155347.0
38	264916.0
39	387514.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.568904649579686	15.042981303528741	18.65683030696408	29.731283739927495
2	45.113429480583214	19.780771605426885	20.83201671758988	14.27378219640002
3	26.839963905203668	19.868095682872386	39.86872892491333	13.423211487010622
4	22.334130162901513	21.995788940427754	39.88671299887599	15.783367897794735
5	20.182120410974086	25.88053888897684	38.81349438789241	15.123846312156664
6	19.750186014849525	30.63321037883705	38.2500356198648	11.36656798644862
7	69.30238890559944	4.497158326341285	23.55033482672915	2.6501179413301252
8	71.45370209128184	3.7371412288061805	22.621622049487865	2.187534630424114
9	66.46483132015135	6.074120980891921	24.02058036633052	3.440467332626213
10	30.657083603780457	23.24055282030174	33.0480789019583	13.054284673959504
11	24.067756898380484	23.7837478430193	37.11380942580778	15.034685832792437
12	19.754112115503347	21.57588614308104	42.134468947393415	16.535532794022195
13	19.842955973847104	21.429227286399545	43.07787293998449	15.649943799768867
14	19.17513891747273	23.07970934190321	41.77250779679263	15.97264394383143
15	18.712808903383095	23.509554039292667	42.26295375749996	15.514683299824275
16	20.816565611790967	22.12345053588108	41.378187977899856	15.681795874428103
17	21.16909145598176	22.2012126585084	40.69789605331898	15.93179983219086
18	20.941947535896908	22.278468187502966	40.79978469770608	15.979799578894044
19	20.62222362942676	23.788877103550902	39.64190162584894	15.946997641173397
20	20.783953646682605	23.015688571564265	40.0187439644118	16.181613817341333
21	20.974242879984804	23.05773584308262	40.29452087324078	15.6735004036918
22	20.278056580176358	21.93417448984438	40.34885304035335	17.43891588962591
23	19.210663795969417	23.737394525622555	40.365443981825955	16.686497696582077
24	18.757515791473395	23.427992464419713	40.98431142843573	16.830180315671157
25	19.404689157313154	24.28343913752434	39.81344689473934	16.498424810423167
26	18.93463359032406	24.184843351750125	39.98176262922095	16.89876042870486
27	19.54115281713553	23.253280985324615	39.94262827109092	17.262937926448938
28	19.047857267243973	23.803315022084316	40.27387718270616	16.874950527965552
29	19.572055028733356	23.517469564804408	39.58699954089952	17.323475865562717
30	19.21022052654076	23.99335095857014	39.112194658603386	17.68423385628572
31	19.659189133566578	24.054458815520764	38.29239951240363	17.99395253850903
32	19.335095857013947	24.98925071635506	37.29732296927193	18.378330457359063
33	19.981572656608673	25.80929915937119	34.859341111656406	19.349787072363732
34	19.46421390916143	26.943688951509493	33.743695283929895	19.84840185539918
35	20.560292557822915	27.235613532382413	32.45264141086327	19.751452498931403
36	20.613548213465894	28.52464103091804	31.151392340937516	19.71041841467855
37	20.650719521269018	28.71024427311729	31.690217993572595	18.948818212041097
38	21.203159877784287	28.17756106827932	31.448066237117484	19.171212816818908
39	21.088099798945652	27.867715737647824	31.36492155714218	19.67926290626435
40	21.313787262336348	27.454715278547344	32.08276473475074	19.148732724365573
41	20.4149001852233	27.44616651099466	31.938765494641192	20.200167809140847
42	20.732787689774725	28.150648281539407	31.3470008073836	19.76956322130226
43	20.482023841562842	27.272024949736412	32.1590704006839	20.086880808016844
44	20.759320531290072	27.594661769594886	31.64012854813431	20.00588915098073
45	20.810169867177482	27.48511089651242	31.39766017065873	20.30705906565137
46	20.89255465670366	27.75075593268637	30.907467506767773	20.449221903842197
47	20.7819272721516	27.585986353634016	31.716814159292035	19.91527221492235
48	20.722275871895135	27.42925894850159	31.46168094099767	20.3867842386056
49	20.608102331913816	27.325090632767107	31.56154321085377	20.505263824465306
50	20.334985039656782	27.12087007456425	31.815409945066254	20.728734940712716
51	20.23417290673928	27.089017999905014	31.65456646666772	21.022242626687987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1099.0
1	1384.5
2	1670.0
3	30209.5
4	58749.0
5	41655.5
6	24562.0
7	24742.5
8	24923.0
9	25309.5
10	25696.0
11	25936.5
12	26177.0
13	25693.5
14	25210.0
15	24571.0
16	23932.0
17	22832.5
18	21733.0
19	20967.0
20	20201.0
21	19615.0
22	19029.0
23	18445.5
24	17862.0
25	18141.0
26	19774.5
27	21129.0
28	22611.5
29	24094.0
30	27281.5
31	30469.0
32	34079.5
33	37690.0
34	41875.0
35	46060.0
36	49723.0
37	53386.0
38	59434.0
39	65482.0
40	70044.0
41	74606.0
42	79107.0
43	83608.0
44	91282.5
45	98957.0
46	100210.0
47	101463.0
48	102395.5
49	103328.0
50	98632.5
51	93937.0
52	88394.0
53	82851.0
54	78295.0
55	73739.0
56	68435.0
57	63131.0
58	58334.0
59	53537.0
60	49159.5
61	44782.0
62	40263.0
63	35744.0
64	30746.5
65	25749.0
66	21369.0
67	16989.0
68	14575.0
69	12161.0
70	10646.0
71	9131.0
72	7997.0
73	6863.0
74	5670.0
75	3681.0
76	2885.0
77	2253.5
78	1622.0
79	1280.0
80	938.0
81	774.0
82	610.0
83	421.0
84	232.0
85	171.0
86	110.0
87	95.0
88	80.0
89	50.0
90	20.0
91	15.5
92	11.0
93	15.0
94	19.0
95	14.5
96	10.0
97	9.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1579175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.45531724695993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7465353035091	29.128570454544928
2	7.864446550073391	6.048595741096636
3	3.751170534960712	4.3275735890808775
4	2.28518736658399	3.5151042060292905
5	1.6230055470280602	3.1206596602269903
6	1.2007784081854485	2.770578877804258
7	0.9335294321165353	2.5129419329990994
8	0.7949869668992603	2.445718081544759
9	0.6277273411285291	2.1725508642919404
>10	5.030950116074386	36.193766420638504
>50	0.11124760744408022	2.7910057260068446
>100	0.027274954302581183	1.8119110446378082
>500	0.002328326511350996	0.6020692408436238
>1k	6.652361461002847E-4	0.3062789031655104
>5k	0.0	0.0
>10k+	1.6630903652507117E-4	2.252675257088906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35223	2.2304684407997843	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.266484081878196E-4	0.0	0.0	0.01576772681938354	0.0
2	1.266484081878196E-4	0.0	0.0	0.07003656972786423	0.0
3	1.266484081878196E-4	0.0	0.0	0.15615748729558154	0.0
4	1.266484081878196E-4	0.0	0.0	0.2610856934791901	0.0
5	1.266484081878196E-4	0.0	0.0	0.5014010480155777	0.0
6	1.266484081878196E-4	0.0	0.0	0.8859056152737981	0.0
7	1.266484081878196E-4	0.0	0.0	1.1785900865958492	0.0
8	1.266484081878196E-4	0.0	0.0	1.6186933050485222	0.0
9	1.266484081878196E-4	0.0	0.0	1.9344277866607564	0.0
10	1.266484081878196E-4	0.0	0.0	2.3618661642946477	0.0
11	1.266484081878196E-4	0.0	0.0	2.7078696154637707	0.0
12	1.266484081878196E-4	0.0	0.0	3.015815219972454	0.0
13	1.266484081878196E-4	0.0	0.0	3.2175028100115566	0.0
14	1.899726122817294E-4	0.0	0.0	3.338895309259582	0.0
15	1.899726122817294E-4	0.0	0.0	3.445976538382383	0.0
16	1.899726122817294E-4	0.0	0.0	3.594408472778508	0.0
17	1.899726122817294E-4	0.0	0.0	3.7593047002390487	0.0
18	1.899726122817294E-4	0.0	0.0	3.9554197603178873	0.0
19	2.532968163756392E-4	0.0	0.0	4.0846011366694635	0.0
20	3.1662102046954896E-4	0.0	0.0	4.2163788053888895	0.0
21	3.1662102046954896E-4	0.0	0.0	4.366457169091456	0.0
22	3.1662102046954896E-4	0.0	0.0	4.536102711859041	0.0
23	3.1662102046954896E-4	0.0	0.0	4.714360346383397	0.0
24	3.1662102046954896E-4	0.0	0.0	4.874887203761458	0.0
25	3.799452245634588E-4	0.0	0.0	5.029841531179255	0.0
26	3.799452245634588E-4	0.0	0.0	5.178020168759004	0.0
27	3.799452245634588E-4	0.0	0.0	5.346969145281555	0.0
28	3.799452245634588E-4	1.899726122817294E-4	0.0	5.525733373438663	0.0
29	3.799452245634588E-4	1.899726122817294E-4	0.0	5.703801035350737	0.0
30	3.799452245634588E-4	1.899726122817294E-4	0.0	5.919926543923251	0.0
31	3.799452245634588E-4	1.899726122817294E-4	0.0	6.130732819351877	0.0
32	3.799452245634588E-4	1.899726122817294E-4	0.0	6.35186094004781	0.0
33	3.799452245634588E-4	1.899726122817294E-4	0.0	6.5683663938448875	0.0
34	4.4326942865736854E-4	1.899726122817294E-4	0.0	6.777399591558884	0.0
35	4.4326942865736854E-4	1.899726122817294E-4	0.0	6.994095017968243	0.0
36	4.4326942865736854E-4	1.899726122817294E-4	0.0	7.210853768581696	0.0
37	5.065936327512784E-4	1.899726122817294E-4	0.0	7.43394493960454	0.0
38	5.065936327512784E-4	1.899726122817294E-4	0.0	7.6857219750819255	0.0
39	5.699178368451882E-4	1.899726122817294E-4	0.0	7.929583485047572	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	25	3.8914703E-5	45.000004	1
ACTCGAC	25	3.8914703E-5	45.000004	28
TAACGCG	70	0.0	45.0	1
CGTTTTT	30345	0.0	44.02867	1
CTCGACG	50	1.0822987E-9	40.500004	1
TCGCACG	45	1.9290383E-8	40.0	1
GCTACGA	120	0.0	39.375004	10
GTTTTTT	34350	0.0	38.9476	2
TCACGAC	35	6.2497747E-6	38.571426	25
ACGGGTA	280	0.0	38.571426	5
TTGCGAG	65	9.094947E-12	38.07692	1
TCGATAG	30	1.1401149E-4	37.500004	1
CGGTCTA	30	1.1401149E-4	37.500004	31
TCGGCGT	110	0.0	36.818184	4
CGTAAGG	270	0.0	36.666668	2
TGGGCGA	695	0.0	36.25899	6
CGATCGC	25	0.0021073464	36.000004	10
AGGGCGA	1580	0.0	35.601265	6
CGCGACC	185	0.0	35.27027	10
CTACGAA	135	0.0	35.000004	11
>>END_MODULE