SRR3553603_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346478
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	4914	1.4182718671892587	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	3496	1.0090106731163306	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	3040	0.8774005853185484	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1634	0.4716028146087198	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1370	0.39540750062053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1109	0.32007804247311517	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	1026	0.2961226975450101	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	953	0.27505353875282124	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.24503720293929196	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	737	0.21271191821702964	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	711	0.2052078342636473	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	708	0.20434197842287244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	522	0.1506589162948297	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCGCCGATCGAATGC	517	0.14921582322687155	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	502	0.14488654402299714	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	459	0.13247594363855714	Illumina Single End Adapter 1 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	370	0.10678888702890227	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	363	0.10476855673376087	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.103902700892986	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC	358	0.10332546366580275	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	354	0.10217098921143622	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	7.026458E-4	45.0	34
TTAGGCG	20	7.026458E-4	45.0	1
TCGCAAA	25	3.8851453E-5	45.0	1
CGTATTA	20	7.026458E-4	45.0	17
CATATCG	20	7.026458E-4	45.0	1
CGTATAT	25	3.8851453E-5	45.0	14
GTCGACC	20	7.026458E-4	45.0	23
CAACGAG	25	3.8851453E-5	45.0	14
CTATCGG	20	7.026458E-4	45.0	30
TTATGTC	20	7.026458E-4	45.0	41
TAGGTGC	20	7.026458E-4	45.0	5
GACCGTA	20	7.026458E-4	45.0	9
CGATTGT	20	7.026458E-4	45.0	10
CGATTAG	25	3.8851453E-5	45.0	10
GCGTTAG	25	3.8851453E-5	45.0	1
CCGTCGA	20	7.026458E-4	45.0	21
CGTTAGG	40	6.7921064E-9	45.0	2
ATATCGG	35	1.2086457E-7	45.0	2
CTAACGG	25	3.8851453E-5	45.0	2
TCGGACG	25	3.8851453E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content