FastQCFastQC Report
Sat 18 Jun 2016
SRR3553602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750028
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.245457502919891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15840.2111921154943549No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8640.11519569936055722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG1250.045.0000042
TGATCCG207.031717E-445.043
AACGTTC207.031717E-445.045
CCTACGT207.031717E-445.029
TTATCGT253.8895047E-545.016
TCGTGCG406.8102963E-945.01
CTTGCGT207.031717E-445.021
TTCGACC207.031717E-445.042
CGATAGA207.031717E-445.010
CAAATCG302.16449E-644.9999961
ACGCATT700.041.78571317
ACCGCTA700.041.78571326
CGAGACA4150.040.12048322
GAACGTT451.9268555E-840.010
TAGACGG1200.039.3749962
TATACGG750.039.02
CGTTGAT356.2453873E-638.57142625
TACGAGA356.2453873E-638.57142624
AACACGT4050.038.33333641
CACGACC4350.038.27586427
Produced by FastQC (version 0.11.5)