##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750028
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.972125040665148	33.0	31.0	34.0	30.0	34.0
2	32.061116384988296	33.0	31.0	34.0	30.0	34.0
3	32.044563669623	33.0	31.0	34.0	30.0	34.0
4	35.70316708176228	37.0	35.0	37.0	33.0	37.0
5	35.62032350792237	37.0	35.0	37.0	33.0	37.0
6	35.600366919635	37.0	35.0	37.0	33.0	37.0
7	35.94214882644381	37.0	35.0	37.0	35.0	37.0
8	35.89505458462884	37.0	35.0	37.0	35.0	37.0
9	37.60475075597178	39.0	38.0	39.0	35.0	39.0
10	36.94852458841537	39.0	37.0	39.0	33.0	39.0
11	36.916036467971864	39.0	37.0	39.0	33.0	39.0
12	36.87173678849323	39.0	37.0	39.0	33.0	39.0
13	36.90346760387612	39.0	37.0	39.0	33.0	39.0
14	37.959996160143355	40.0	37.0	41.0	33.0	41.0
15	38.10369612867786	40.0	37.0	41.0	33.0	41.0
16	38.125341987232474	40.0	37.0	41.0	33.0	41.0
17	38.08144095953751	40.0	37.0	41.0	33.0	41.0
18	38.02689899577082	40.0	37.0	41.0	33.0	41.0
19	37.95088583359554	40.0	37.0	41.0	33.0	41.0
20	37.920442970129116	40.0	36.0	41.0	33.0	41.0
21	37.76430079943682	40.0	36.0	41.0	33.0	41.0
22	37.792941063533625	40.0	36.0	41.0	33.0	41.0
23	37.746152143653305	40.0	36.0	41.0	33.0	41.0
24	37.69386742894932	40.0	36.0	41.0	33.0	41.0
25	37.57754110513208	40.0	36.0	41.0	33.0	41.0
26	37.56730282069469	40.0	36.0	41.0	33.0	41.0
27	37.53372274101767	40.0	36.0	41.0	33.0	41.0
28	37.42599342957863	39.0	36.0	41.0	32.0	41.0
29	37.39234668572373	39.0	35.0	41.0	32.0	41.0
30	37.21328537067949	39.0	35.0	41.0	32.0	41.0
31	37.14072407963436	39.0	35.0	41.0	31.0	41.0
32	37.01194755395799	39.0	35.0	41.0	31.0	41.0
33	36.926110758531685	39.0	35.0	41.0	31.0	41.0
34	36.78959718837164	39.0	35.0	41.0	31.0	41.0
35	36.693060792397084	39.0	35.0	41.0	30.0	41.0
36	36.53265878073885	39.0	35.0	41.0	30.0	41.0
37	36.47932077202451	39.0	35.0	41.0	30.0	41.0
38	36.384128325875835	39.0	35.0	41.0	30.0	41.0
39	36.303187347672356	39.0	35.0	41.0	30.0	41.0
40	36.22453828390407	39.0	35.0	41.0	29.0	41.0
41	36.06838811351043	39.0	35.0	41.0	29.0	41.0
42	35.95937618328916	39.0	35.0	40.0	29.0	41.0
43	35.89879177844027	38.0	35.0	40.0	28.0	41.0
44	35.8058565813543	38.0	35.0	40.0	28.0	41.0
45	35.74458153562267	38.0	35.0	40.0	28.0	41.0
46	35.56835878127217	38.0	34.0	40.0	27.0	41.0
47	35.46816385521607	38.0	34.0	40.0	27.0	41.0
48	35.373492722938344	38.0	34.0	40.0	27.0	41.0
49	35.28645463902681	38.0	34.0	40.0	27.0	41.0
50	35.16641912035284	38.0	34.0	40.0	26.0	41.0
51	33.69723663649891	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	2.0
12	8.0
13	13.0
14	31.0
15	52.0
16	104.0
17	159.0
18	298.0
19	559.0
20	902.0
21	1332.0
22	2021.0
23	2918.0
24	4074.0
25	5628.0
26	7299.0
27	8627.0
28	9887.0
29	11523.0
30	14118.0
31	17010.0
32	22212.0
33	30083.0
34	47718.0
35	57931.0
36	62256.0
37	88391.0
38	147312.0
39	207500.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.14507725044932	18.72370098183001	22.08904200909833	13.042179758622344
2	32.38772419163018	24.141498717381218	25.987563130976444	17.483213960012158
3	32.65438090311295	25.044931655884845	26.425013466163932	15.875673974838273
4	27.12952049790141	27.586703429738623	25.966363922413567	19.317412149946403
5	24.54388369500872	31.612553131349763	24.8344061821692	19.009156991472317
6	23.750579978347474	37.34393915960471	24.862671793586372	14.042809068461445
7	84.13979211442772	5.817382817708139	6.74321491997632	3.2996101478878126
8	86.80342600542913	4.790487821787987	5.683654476899529	2.7224316958833534
9	81.2908318089458	7.584516844704464	7.20106449359224	3.923586852757497
10	38.504162511266244	30.478728794125022	16.134730970043783	14.882377724564948
11	30.146341203261745	28.412672593556508	23.71831451625806	17.72267168692369
12	25.271589860645204	26.779000250657308	28.628797858213296	19.320612030484195
13	25.117195624696677	26.711136117584942	29.835286149317092	18.336382108401285
14	22.73235132555051	28.698528588266036	29.519697931277232	19.049422154906217
15	22.427829361037187	27.352445508701006	31.207634914963172	19.01209021529863
16	25.353586799426154	26.49327758430352	29.05851514876778	19.094620467502548
17	25.548379527164318	26.337016751374616	27.515506087772724	20.59909763368834
18	26.833798204867023	25.81476958193561	27.668833696875318	19.68259851632206
19	25.47171572261302	28.16841504583829	26.21822118640904	20.141648045139647
20	26.151290351826866	27.982955303002022	26.454079047715553	19.41167529745556
21	25.596244406875478	27.557504519831262	27.88282570784024	18.963425365453023
22	24.703211080119676	25.47211570768025	28.135216285258686	21.689456926941393
23	23.019540603817457	28.37614062408337	27.843627171252272	20.7606916008469
24	22.435962390737412	27.6497677420043	29.112913117910267	20.801356749348024
25	23.460990789677187	28.997584090193968	26.564341597913675	20.97708352221517
26	22.870079517031364	28.884654972881012	26.79446633992331	21.450799170164313
27	22.55502461241447	28.077218450511182	27.675100129595158	21.69265680747919
28	21.488397766483384	29.41730175406785	28.362007818374778	20.732292661073988
29	23.50298922173572	27.99162164612521	27.258982331326298	21.24640680081277
30	23.595385772264503	27.16591913901881	28.38814017610009	20.850554912616595
31	24.782141466718578	27.950823169268347	26.581540955804318	20.68549440820876
32	23.587252742564278	28.215346627059258	27.039123872708753	21.158276757667714
33	23.707914904510233	27.434842432549182	26.201688470297107	22.655554192643475
34	22.89381196435333	28.70732825974497	27.89709184190457	20.50176793399713
35	22.744084220855754	29.415168500375987	26.574874538017244	21.265872740751014
36	24.397089175337456	30.765118102257517	25.211192115494356	19.626600606910674
37	23.3199293893028	30.0402118320916	25.683841136597568	20.95601764200803
38	22.99580815649549	30.08007701045828	25.827569104086777	21.096545728959452
39	23.609918563040313	27.89615854341438	25.46278272277835	23.03114017076696
40	25.204525697707286	27.27191518183321	26.436346376401946	21.087212744057553
41	22.287567930797252	28.362274475086263	26.574741209661507	22.77541638445498
42	23.44992453615065	28.589199336558103	26.549542150426387	21.411333976864864
43	22.241036334643507	28.14254934482446	27.448308596479066	22.16810572405297
44	23.025940364893042	27.973355661388645	27.00419184350451	21.996512130213805
45	23.31739615054371	28.244012223543653	26.096892382684377	22.341699243228252
46	23.437391670710962	28.29854352104188	25.776504343837832	22.487560464409327
47	21.954380369799527	29.243574906536825	27.462041417120425	21.340003306543224
48	22.634488312436336	29.066648178468	26.87939650253057	21.41946700656509
49	23.093271184542445	28.774259094327142	26.805532593449843	21.326937127680566
50	22.453295076983792	28.603865455689654	26.876863263771483	22.065976203555067
51	21.97291301124758	27.901491677644035	26.034494712197407	24.091100598910977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1877.0
1	2031.0
2	2185.0
3	2976.0
4	3767.0
5	2676.0
6	1585.0
7	1662.5
8	1740.0
9	1834.5
10	1929.0
11	1986.0
12	2043.0
13	2075.5
14	2108.0
15	2224.0
16	2340.0
17	2208.5
18	2077.0
19	2360.5
20	2644.0
21	2843.5
22	3043.0
23	3254.5
24	3466.0
25	4563.5
26	6312.0
27	6963.0
28	8750.0
29	10537.0
30	12291.5
31	14046.0
32	15631.0
33	17216.0
34	20896.5
35	24577.0
36	24954.0
37	25331.0
38	28314.5
39	31298.0
40	34700.0
41	38102.0
42	40114.5
43	42127.0
44	44846.0
45	47565.0
46	51809.0
47	56053.0
48	57729.0
49	59405.0
50	60398.5
51	61392.0
52	58682.5
53	55973.0
54	51883.0
55	47793.0
56	43985.0
57	40177.0
58	36543.5
59	32910.0
60	29940.0
61	26970.0
62	24433.5
63	21897.0
64	19129.0
65	16361.0
66	13439.5
67	10518.0
68	9160.0
69	7802.0
70	6890.0
71	5978.0
72	4996.0
73	4014.0
74	3670.0
75	2732.5
76	2139.0
77	1779.5
78	1420.0
79	1087.0
80	754.0
81	633.0
82	512.0
83	344.0
84	176.0
85	141.0
86	106.0
87	62.5
88	19.0
89	24.5
90	30.0
91	21.5
92	13.0
93	16.5
94	20.0
95	16.5
96	13.0
97	20.5
98	28.0
99	15.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	750028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.568463829042134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70967516569844	20.044940498269604
2	8.471045199076208	4.67067406329827
3	3.5417412816008165	2.929210992409123
4	2.0526856002295797	2.2635755488929923
5	1.444029685777217	1.9904840080206143
6	1.1195908047436305	1.8519239162341772
7	0.930583463035765	1.7958329578424377
8	0.7887620592931345	1.739596664107485
9	0.6506764420539648	1.61443349614571
>10	7.511366053885187	45.580162004610756
>50	0.7331702748548918	12.762213547468292
>100	0.0442430338266516	2.0177529274565704
>500	0.0014585615547247782	0.28013176686490177
>1k	9.723743698165188E-4	0.45906760837908545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1841	0.245457502919891	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1584	0.2111921154943549	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	864	0.11519569936055722	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026265686081052973	0.0
2	0.0	0.0	0.0	0.16292725071597328	0.0
3	0.0	0.0	0.0	0.2406576821132011	0.0
4	0.0	0.0	0.0	0.3538534561376375	0.0
5	0.0	0.0	0.0	0.6779746889449461	0.0
6	0.0	0.0	0.0	1.0194286079986348	0.0
7	0.0	0.0	0.0	1.2615529020249912	0.0
8	0.0	0.0	0.0	1.668071058680476	0.0
9	0.0	0.0	0.0	1.8717967862533131	0.0
10	0.0	0.0	0.0	2.302847360365213	0.0
11	0.0	0.0	0.0	2.8292277088322035	0.0
12	0.0	0.0	0.0	3.2485453876388615	0.0
13	0.0	0.0	0.0	3.4360050558112496	0.0
14	0.0	0.0	0.0	3.5226684870431506	0.0
15	0.0	0.0	0.0	3.6461305444596737	0.0
16	0.0	0.0	0.0	3.9050542113094444	0.0
17	0.0	0.0	0.0	4.221709056195236	0.0
18	0.0	0.0	0.0	4.60702800428784	0.0
19	0.0	0.0	0.0	4.813820284042729	0.0
20	0.0	0.0	0.0	5.032878772525826	0.0
21	0.0	0.0	0.0	5.306468558507149	0.0
22	0.0	0.0	0.0	5.587791389121473	0.0
23	0.0	0.0	0.0	5.894313278970919	0.0
24	0.0	0.0	0.0	6.1339043342381885	0.0
25	1.3332835574138564E-4	0.0	0.0	6.371095479102114	0.0
26	1.3332835574138564E-4	0.0	0.0	6.591753907854107	0.0
27	1.3332835574138564E-4	0.0	0.0	6.832678246678791	0.0
28	1.3332835574138564E-4	0.0	0.0	7.0756025108395955	0.0
29	1.3332835574138564E-4	0.0	0.0	7.345192446148677	0.0
30	1.3332835574138564E-4	0.0	0.0	7.666513783485416	0.0
31	2.666567114827713E-4	0.0	0.0	7.927304047315567	0.0
32	2.666567114827713E-4	0.0	0.0	8.208226892862667	0.0
33	2.666567114827713E-4	0.0	0.0	8.493949559216455	0.0
34	2.666567114827713E-4	0.0	0.0	8.771939180937245	0.0
35	2.666567114827713E-4	0.0	0.0	9.078727727498173	0.0
36	2.666567114827713E-4	0.0	0.0	9.357783976064894	0.0
37	2.666567114827713E-4	0.0	0.0	9.637240209698838	0.0
38	2.666567114827713E-4	0.0	0.0	9.951628472537026	0.0
39	2.666567114827713E-4	0.0	0.0	10.406411493970891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	125	0.0	45.000004	2
TGATCCG	20	7.031717E-4	45.0	43
AACGTTC	20	7.031717E-4	45.0	45
CCTACGT	20	7.031717E-4	45.0	29
TTATCGT	25	3.8895047E-5	45.0	16
TCGTGCG	40	6.8102963E-9	45.0	1
CTTGCGT	20	7.031717E-4	45.0	21
TTCGACC	20	7.031717E-4	45.0	42
CGATAGA	20	7.031717E-4	45.0	10
CAAATCG	30	2.16449E-6	44.999996	1
ACGCATT	70	0.0	41.785713	17
ACCGCTA	70	0.0	41.785713	26
CGAGACA	415	0.0	40.120483	22
GAACGTT	45	1.9268555E-8	40.0	10
TAGACGG	120	0.0	39.374996	2
TATACGG	75	0.0	39.0	2
CGTTGAT	35	6.2453873E-6	38.571426	25
TACGAGA	35	6.2453873E-6	38.571426	24
AACACGT	405	0.0	38.333336	41
CACGACC	435	0.0	38.275864	27
>>END_MODULE