FastQCFastQC Report
Sat 18 Jun 2016
SRR3553598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161344
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.2844118538520886No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13390.11529744847349277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11900.10246748594731621No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA302.1655214E-645.00000425
ACGCTAG253.8908307E-545.01
GCGATAT650.041.5384649
TAGTACG501.0822987E-940.51
ACGCATT451.9283107E-840.017
GCGAGAC4700.039.25531821
TTACGGG2050.038.4146353
CGTAAGG1950.038.0769232
TACGGGT1550.037.7419364
GACACGA4850.037.5773225
AACACGT4950.037.27272841
ACGACCA4650.037.25806428
CGAGACA4900.037.1938822
CGAATAT2300.037.17391214
GTAACGG1150.037.1739122
CACGACC4950.036.8181827
TCAAGCG5250.036.42857417
TACGAAT2350.036.3829812
GGCGATA2600.036.3461578
ACACGAC5050.036.0891126
Produced by FastQC (version 0.11.5)