##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1161344
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993353390554393	33.0	31.0	34.0	30.0	34.0
2	32.09400832139315	33.0	31.0	34.0	30.0	34.0
3	32.08403281026121	33.0	31.0	34.0	30.0	34.0
4	35.73579146230574	37.0	35.0	37.0	33.0	37.0
5	35.636068210624934	37.0	35.0	37.0	33.0	37.0
6	35.597002266339686	37.0	35.0	37.0	33.0	37.0
7	35.887166076545796	37.0	35.0	37.0	35.0	37.0
8	35.809704962526176	37.0	35.0	37.0	35.0	37.0
9	37.50457487187259	39.0	37.0	39.0	35.0	39.0
10	36.96341652430288	39.0	37.0	39.0	33.0	39.0
11	36.88490318114185	39.0	37.0	39.0	33.0	39.0
12	36.797084240328445	39.0	35.0	39.0	33.0	39.0
13	36.774299432381795	39.0	35.0	39.0	33.0	39.0
14	37.811799949024575	40.0	37.0	41.0	33.0	41.0
15	37.97323704259892	40.0	37.0	41.0	33.0	41.0
16	38.01116723382564	40.0	37.0	41.0	33.0	41.0
17	37.962670836548	40.0	37.0	41.0	33.0	41.0
18	37.918644260443074	40.0	37.0	41.0	33.0	41.0
19	37.864552621789926	40.0	36.0	41.0	33.0	41.0
20	37.80224205747823	40.0	36.0	41.0	33.0	41.0
21	37.66282772374077	40.0	36.0	41.0	33.0	41.0
22	37.67464420533451	40.0	36.0	41.0	33.0	41.0
23	37.60683053427753	40.0	36.0	41.0	33.0	41.0
24	37.55918487545465	40.0	35.0	41.0	33.0	41.0
25	37.39687121128623	39.0	35.0	41.0	32.0	41.0
26	37.367750640637055	39.0	35.0	41.0	32.0	41.0
27	37.318877094125426	39.0	35.0	41.0	32.0	41.0
28	37.200194774330434	39.0	35.0	41.0	32.0	41.0
29	37.15277213297696	39.0	35.0	41.0	32.0	41.0
30	36.996274144439546	39.0	35.0	41.0	31.0	41.0
31	36.90496355946214	39.0	35.0	41.0	31.0	41.0
32	36.77390075636504	39.0	35.0	41.0	31.0	41.0
33	36.69629584343657	39.0	35.0	41.0	31.0	41.0
34	36.5302726840626	39.0	35.0	41.0	30.0	41.0
35	36.438209522759834	39.0	35.0	41.0	30.0	41.0
36	36.27635739281384	39.0	35.0	41.0	30.0	41.0
37	36.192268612917445	39.0	35.0	41.0	29.0	41.0
38	36.069286964069214	39.0	35.0	41.0	29.0	41.0
39	35.96283099581175	39.0	35.0	40.0	29.0	41.0
40	35.82555211892428	38.0	35.0	40.0	28.0	41.0
41	35.70201507908079	38.0	35.0	40.0	28.0	41.0
42	35.562415615011574	38.0	34.0	40.0	27.0	41.0
43	35.51177945552739	38.0	34.0	40.0	27.0	41.0
44	35.389951642235204	38.0	34.0	40.0	27.0	41.0
45	35.30505173316433	38.0	34.0	40.0	27.0	41.0
46	35.14015829934972	38.0	34.0	40.0	26.0	41.0
47	35.056630076876445	37.0	34.0	40.0	26.0	41.0
48	34.9458377534994	37.0	34.0	40.0	26.0	41.0
49	34.86079318444836	37.0	34.0	40.0	26.0	41.0
50	34.72888480932437	37.0	34.0	40.0	25.0	41.0
51	33.228693651493444	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	9.0
11	8.0
12	16.0
13	35.0
14	49.0
15	80.0
16	189.0
17	320.0
18	560.0
19	954.0
20	1674.0
21	2576.0
22	3608.0
23	5115.0
24	6951.0
25	9171.0
26	12063.0
27	14730.0
28	17161.0
29	19874.0
30	23730.0
31	29202.0
32	36468.0
33	49998.0
34	81209.0
35	91144.0
36	97107.0
37	137763.0
38	219982.0
39	299526.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58174666593189	18.40462429736581	23.291376198611264	14.722252838091041
2	31.18128650942357	24.881430480546676	25.442935082111763	18.494347927918
3	33.440307092472175	23.521454452771962	25.756106717733935	17.282131737021935
4	28.50163259120467	25.905760911495644	25.396351124214707	20.196255373084977
5	25.202437865094236	29.922830789154638	25.023335032514055	19.85139631323708
6	25.215353934751462	35.74668659759727	24.253795602336602	14.784163865314671
7	83.53769425768765	5.6847066846687975	6.963483687865095	3.814115369778463
8	85.60934572357544	4.844387192769757	6.285390030860796	3.260877052794004
9	80.29653573790367	6.704301223410118	8.038272897608287	4.960890141077924
10	42.35067301333627	26.143847128843824	15.60054557478232	15.904934283037583
11	31.71170643668026	26.668325664058194	22.32637358095448	19.293594318307065
12	27.597679929461037	24.023200705389616	28.322185330100297	20.056934035049046
13	25.47866954149675	24.15218918769977	29.420309572357546	20.94883169844594
14	22.321465474484736	26.338363137881625	29.362876115948417	21.97729527168522
15	22.701542350931334	24.99664182188912	30.645527940041884	21.656287887137662
16	25.45042640251295	24.382697977515704	28.760384520004408	21.406491099966935
17	25.959750082662847	23.962925713655903	27.045130469524963	23.032193734156287
18	26.21634933318638	24.190248539623056	27.542829686983357	22.05057244020721
19	26.47148476248209	25.77100325140527	25.639001019508434	22.11851096660421
20	26.799122396120357	25.75369571806459	26.2362400804585	21.210941805356555
21	26.640599167860685	25.659236195304754	26.858708530805686	20.84145610602888
22	25.666813622836987	23.631585473382565	26.82004642896506	23.881554474815385
23	24.461227681031634	26.05162639149124	27.287091507770306	22.20005441970682
24	24.010284635732393	25.73699093464124	27.722018626694588	22.530705802931774
25	25.131830017634744	25.848671883610713	26.599956602005953	22.419541496748593
26	23.502510883941362	26.212216190896065	25.805876639479774	24.479396285682796
27	23.783822881075718	25.15981483522539	27.570986718836103	23.485375564862778
28	22.794451945332305	26.82194078584812	27.063040752782978	23.320566516036592
29	23.75618249200926	26.301767607186154	26.55914182188912	23.382908078915463
30	24.81521409677064	25.357000165325694	27.1420870990852	22.685698638818472
31	25.611618951835112	26.214713297696463	25.25995742863441	22.913710321834014
32	25.091187451780005	26.351881957456193	25.62970144935523	22.927229141408574
33	25.594655847018625	25.528353356111538	25.142593409015763	23.734397387854074
34	23.20475242477681	26.725586906205223	26.619502920753884	23.45015774826408
35	24.28823845475587	25.872437451780005	26.368931169403726	23.4703929240604
36	24.54199616995481	28.307202689297917	24.679250936845587	22.471550203901685
37	24.001415601234434	28.249597018626694	24.969259754215805	22.77972762592307
38	24.21418632205445	27.22518048054668	26.226854403174254	22.333778794224624
39	24.2679171718285	26.153146698997027	25.59456973988758	23.984366389286897
40	24.841821200264523	25.31317163562218	27.275467045078805	22.5695401190345
41	22.677001818582607	25.850910669017967	27.18505455747823	24.287032954921195
42	23.555380662404936	26.281101895734597	26.613130993056323	23.550386448804144
43	23.536350986443296	24.904593298798634	26.643526810316327	24.91552890444175
44	23.807416234982917	25.37396327014218	26.549239501818583	24.269380993056323
45	23.71812314008597	24.976751074616995	26.47845944009699	24.826666345200042
46	23.548405984790037	25.582256420147694	26.86706092251736	24.002276672544916
47	23.10297379587788	26.41155419927257	27.438209522759838	23.047262482089717
48	22.693276066350712	26.60064545905434	27.26918122451229	23.436897250082662
49	23.80991334178331	26.650501487931223	26.643785131709468	22.895800038575995
50	22.59166965171388	26.109920919210843	28.015902264961973	23.2825071641133
51	22.649017000991954	25.845313705499834	26.944299019067564	24.561370274440648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1593.0
1	2094.5
2	2596.0
3	4419.0
4	6242.0
5	4424.5
6	2607.0
7	2505.5
8	2404.0
9	2481.5
10	2559.0
11	2625.0
12	2691.0
13	2724.5
14	2758.0
15	2782.0
16	2806.0
17	2678.0
18	2550.0
19	2598.0
20	2646.0
21	3080.5
22	3515.0
23	4009.0
24	4503.0
25	5577.5
26	7900.0
27	9148.0
28	11986.0
29	14824.0
30	16761.0
31	18698.0
32	21709.0
33	24720.0
34	28656.0
35	32592.0
36	34437.5
37	36283.0
38	37774.0
39	39265.0
40	42638.5
41	46012.0
42	49260.0
43	52508.0
44	56716.0
45	60924.0
46	68039.0
47	75154.0
48	79845.5
49	84537.0
50	84083.0
51	83629.0
52	81100.0
53	78571.0
54	75316.5
55	72062.0
56	70491.5
57	68921.0
58	65475.0
59	62029.0
60	58269.5
61	54510.0
62	51945.0
63	49380.0
64	45196.0
65	41012.0
66	36223.5
67	31435.0
68	27825.5
69	24216.0
70	21016.5
71	17817.0
72	15586.0
73	13355.0
74	11385.5
75	7921.0
76	6426.0
77	5119.0
78	3812.0
79	2975.0
80	2138.0
81	1579.0
82	1020.0
83	725.0
84	430.0
85	310.5
86	191.0
87	132.5
88	74.0
89	54.0
90	34.0
91	33.0
92	32.0
93	26.5
94	21.0
95	23.5
96	26.0
97	13.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1161344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.11753801191349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50567242353382	16.99270176342304
2	7.8781335061489015	3.64246101582653
3	3.0876628535090584	2.1413749015190673
4	1.7248308960189056	1.594953752113594
5	1.2266569995144985	1.4178644906928082
6	0.9340656800560282	1.2955979318591433
7	0.7526208293654426	1.217911844198943
8	0.6559263400732053	1.213072167972609
9	0.5400836717676399	1.1236864330532
>10	8.353372897501812	47.424980728937285
>50	1.276796317294236	18.643389805724166
>100	0.05967389502583726	2.2449630666021374
>500	0.0030024601270860142	0.44526185344161684
>1k	0.0015012300635430071	0.6017802446358251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3303	0.2844118538520886	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1339	0.11529744847349277	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1190	0.10246748594731621	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7221426209632976E-4	0.0	0.0	0.022646175465667363	0.0
2	1.7221426209632976E-4	0.0	0.0	0.13088283919321061	0.0
3	1.7221426209632976E-4	0.0	0.0	0.19899357985230906	0.0
4	1.7221426209632976E-4	0.0	0.0	0.2929364598258569	0.0
5	1.7221426209632976E-4	0.0	0.0	0.5749373140085969	0.0
6	2.5832139314449466E-4	0.0	0.0	0.9041248760057313	0.0
7	2.5832139314449466E-4	0.0	0.0	1.1472914140857489	0.0
8	3.444285241926595E-4	0.0	0.0	1.5293487545464566	0.0
9	3.444285241926595E-4	0.0	0.0	1.7062128017193872	0.0
10	3.444285241926595E-4	0.0	0.0	2.0754401796539184	0.0
11	3.444285241926595E-4	0.0	0.0	2.4791965171387633	0.0
12	4.3053565524082445E-4	0.0	0.0	2.7989984018516476	0.0
13	4.3053565524082445E-4	0.0	0.0	2.9332394191557367	0.0
14	4.3053565524082445E-4	0.0	0.0	2.9888646258128513	0.0
15	5.166427862889893E-4	0.0	0.0	3.0845296484073623	0.0
16	5.166427862889893E-4	0.0	0.0	3.279562300231456	0.0
17	5.166427862889893E-4	0.0	0.0	3.5002548771079027	0.0
18	5.166427862889893E-4	0.0	0.0	3.779328088835005	0.0
19	5.166427862889893E-4	0.0	0.0	3.9257963187479334	0.0
20	5.166427862889893E-4	0.0	0.0	4.073383941364488	0.0
21	5.166427862889893E-4	0.0	0.0	4.260408630001102	0.0
22	5.166427862889893E-4	0.0	0.0	4.457593960101399	0.0
23	6.027499173371542E-4	0.0	0.0	4.6525405047944455	0.0
24	6.027499173371542E-4	0.0	0.0	4.8147663396891875	0.0
25	6.027499173371542E-4	0.0	0.0	4.975700567618208	0.0
26	6.027499173371542E-4	0.0	0.0	5.122857654579522	0.0
27	6.027499173371542E-4	0.0	0.0	5.288872203240383	0.0
28	6.88857048385319E-4	0.0	0.0	5.452131323707704	0.0
29	7.74964179433484E-4	0.0	0.0	5.630545299239502	0.0
30	7.74964179433484E-4	0.0	0.0	5.8409911275212165	0.0
31	8.610713104816489E-4	0.0	0.0	6.008125068885705	0.0
32	8.610713104816489E-4	0.0	0.0	6.194977543260222	0.0
33	8.610713104816489E-4	0.0	0.0	6.373563733054117	0.0
34	8.610713104816489E-4	0.0	0.0	6.566529813733054	0.0
35	8.610713104816489E-4	0.0	0.0	6.769742643006723	0.0
36	8.610713104816489E-4	0.0	0.0	6.9553035104155185	0.0
37	8.610713104816489E-4	0.0	0.0	7.152230519122671	0.0
38	0.0010332855725779786	0.0	0.0	7.38454755869062	0.0
39	0.0010332855725779786	0.0	0.0	7.702368979389397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	30	2.1655214E-6	45.000004	25
ACGCTAG	25	3.8908307E-5	45.0	1
GCGATAT	65	0.0	41.538464	9
TAGTACG	50	1.0822987E-9	40.5	1
ACGCATT	45	1.9283107E-8	40.0	17
GCGAGAC	470	0.0	39.255318	21
TTACGGG	205	0.0	38.414635	3
CGTAAGG	195	0.0	38.076923	2
TACGGGT	155	0.0	37.741936	4
GACACGA	485	0.0	37.57732	25
AACACGT	495	0.0	37.272728	41
ACGACCA	465	0.0	37.258064	28
CGAGACA	490	0.0	37.19388	22
CGAATAT	230	0.0	37.173912	14
GTAACGG	115	0.0	37.173912	2
CACGACC	495	0.0	36.81818	27
TCAAGCG	525	0.0	36.428574	17
TACGAAT	235	0.0	36.38298	12
GGCGATA	260	0.0	36.346157	8
ACACGAC	505	0.0	36.08911	26
>>END_MODULE