FastQCFastQC Report
Sat 18 Jun 2016
SRR3553597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051874
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27130.25792062547415373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15770.1499228995107779No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.033014E-445.01
CGTAAAT207.033014E-445.030
CAATGCG351.2117926E-745.01
ACGGGCT2300.040.1086925
CGTATGG1350.040.02
CGAATGG1100.038.8636362
TCGTTTA356.2477848E-638.57142638
TATAGCG700.038.5714261
TCGACTA356.2477848E-638.5714261
TCGACAG356.2477848E-638.5714261
TAGGGCG4500.038.05
CGTTTTT21350.037.7283361
CGTAAGG1500.037.52
TCGTTAG301.1398561E-437.4999961
CTCGTAT301.1398561E-437.4999969
TACGTAC301.1398561E-437.49999640
GTTACGG1100.036.818182
ATGCGAG552.748493E-936.818181
TCGTTGA1300.036.34615324
TAATCGT1550.036.29032521
Produced by FastQC (version 0.11.5)