##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973909422611452	33.0	31.0	34.0	30.0	34.0
2	32.068142191935536	33.0	31.0	34.0	30.0	34.0
3	32.054287870980744	33.0	31.0	34.0	30.0	34.0
4	35.706244284011206	37.0	35.0	37.0	33.0	37.0
5	35.6125591087906	37.0	35.0	37.0	33.0	37.0
6	35.59153853028024	37.0	35.0	37.0	33.0	37.0
7	35.92187372251809	37.0	35.0	37.0	35.0	37.0
8	35.85814365598922	37.0	35.0	37.0	35.0	37.0
9	37.55653243639447	39.0	37.0	39.0	35.0	39.0
10	36.89629556391735	39.0	37.0	39.0	32.0	39.0
11	36.89052396009408	39.0	37.0	39.0	33.0	39.0
12	36.89162009898524	39.0	37.0	39.0	33.0	39.0
13	36.91677995653472	39.0	37.0	39.0	33.0	39.0
14	38.040352741868325	40.0	37.0	41.0	33.0	41.0
15	38.17097199854735	40.0	37.0	41.0	33.0	41.0
16	38.18068608977882	40.0	37.0	41.0	33.0	41.0
17	38.11518679993992	40.0	37.0	41.0	33.0	41.0
18	38.072618963868294	40.0	37.0	41.0	33.0	41.0
19	37.984092201157175	40.0	37.0	41.0	33.0	41.0
20	37.934529230687325	40.0	37.0	41.0	33.0	41.0
21	37.81333315587228	40.0	36.0	41.0	33.0	41.0
22	37.830189737554115	40.0	36.0	41.0	33.0	41.0
23	37.746938321509994	40.0	36.0	41.0	33.0	41.0
24	37.71948161091537	40.0	36.0	41.0	33.0	41.0
25	37.60334697882066	40.0	36.0	41.0	33.0	41.0
26	37.57777737637778	40.0	36.0	41.0	33.0	41.0
27	37.53372457157416	40.0	36.0	41.0	33.0	41.0
28	37.426549187450206	40.0	36.0	41.0	32.0	41.0
29	37.39405004781941	39.0	36.0	41.0	32.0	41.0
30	37.24463101093857	39.0	35.0	41.0	32.0	41.0
31	37.16816652945125	39.0	35.0	41.0	31.0	41.0
32	37.03374548662673	39.0	35.0	41.0	31.0	41.0
33	36.91641109106224	39.0	35.0	41.0	31.0	41.0
34	36.8019639234357	39.0	35.0	41.0	31.0	41.0
35	36.711391288310196	39.0	35.0	41.0	30.0	41.0
36	36.57209893960683	39.0	35.0	41.0	30.0	41.0
37	36.52768772685702	39.0	35.0	41.0	30.0	41.0
38	36.42190984851798	39.0	35.0	41.0	30.0	41.0
39	36.34425701177137	39.0	35.0	41.0	30.0	41.0
40	36.235751620441235	39.0	35.0	41.0	30.0	41.0
41	36.11762150219513	39.0	35.0	41.0	29.0	41.0
42	36.01887773630682	39.0	35.0	40.0	29.0	41.0
43	35.932895004534764	39.0	35.0	40.0	29.0	41.0
44	35.8606382513495	38.0	35.0	40.0	28.0	41.0
45	35.78797555600766	38.0	35.0	40.0	28.0	41.0
46	35.66824543624046	38.0	35.0	40.0	28.0	41.0
47	35.562671004321814	38.0	34.0	40.0	28.0	41.0
48	35.46512319916644	38.0	34.0	40.0	27.0	41.0
49	35.362915140026274	38.0	34.0	40.0	27.0	41.0
50	35.237077824910585	38.0	34.0	40.0	27.0	41.0
51	33.76685705702394	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	10.0
11	9.0
12	15.0
13	18.0
14	35.0
15	56.0
16	125.0
17	243.0
18	404.0
19	745.0
20	1378.0
21	2137.0
22	3272.0
23	4339.0
24	5939.0
25	7847.0
26	10054.0
27	11774.0
28	13164.0
29	15270.0
30	18756.0
31	23916.0
32	30699.0
33	40932.0
34	65146.0
35	80987.0
36	86893.0
37	126045.0
38	208364.0
39	293213.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4855562548366	18.681800291669916	23.615471054517936	13.217172398975544
2	32.11506321099295	24.92941169759876	25.432989122271298	17.52253596913699
3	32.16392837925455	24.684325308924834	26.984600817208143	16.167145494612473
4	27.54797627852766	27.07168349060819	26.21540222498132	19.164938005882835
5	24.894521587186297	31.414694155383632	25.110802244375275	18.579982013054796
6	23.899725632537738	37.4754010461329	24.86809256621991	13.756780755109451
7	83.55744129049677	5.859256907196109	7.419805033682741	3.163496768624379
8	85.98178108784893	5.298068019553673	5.836725691480158	2.883425201117244
9	80.1935402909474	7.583702991042654	7.800458990335344	4.422297727674607
10	38.08326852835986	28.191209213270792	17.850712157539782	15.874810100829567
11	30.512970184641887	27.632777309829887	22.913866109438963	18.940386396089266
12	25.45352390115166	25.55667313765717	28.997484489587155	19.992318471604015
13	24.736898145595383	26.829354086135794	29.3513291515904	19.082418616678424
14	22.780390046716622	28.971720947565966	28.72596907994684	19.52191992577058
15	22.624667973540557	27.372670110678655	30.639886526333004	19.362775389447787
16	25.09397513390387	25.953583794256723	29.21633199413618	19.736109077703222
17	25.217564080869003	25.801569389489615	27.913799561544444	21.06706696809694
18	25.9858119888884	25.22155695454018	28.125516934537785	20.667114122033627
19	24.827498350562898	27.937661735150787	26.85454721763253	20.380292696653783
20	25.796815968452496	26.555081692293943	27.258397868946282	20.38970447030728
21	24.987878776355345	27.771006793589347	27.45138676305337	19.78972766700194
22	25.096541981263915	25.278502938564884	27.196413258622233	22.42854182154897
23	23.06331366684603	28.1766637448972	27.259348553153707	21.500674035103064
24	22.375493642774703	28.097852024101744	28.337804718055587	21.188849615067966
25	23.113319656156538	28.569581527825576	27.476865099812336	20.840233716205553
26	23.042113409020473	28.17694895015943	26.95636549624765	21.82457214457245
27	23.574306428336474	27.191659837585107	27.98472060341828	21.249313130660134
28	22.734471999498037	28.237697671013827	28.687656506387647	20.340173823100486
29	23.5263919442823	27.11028126942961	27.414595284226056	21.94873150206203
30	23.069017772090575	28.224673297372117	27.458326757767566	21.247982172769742
31	24.115911221305975	28.092338055698683	26.32891391934776	21.462836803647587
32	24.184645689502734	27.677554535999562	27.355462726524284	20.782337047973424
33	23.895637690445813	27.64456579400194	26.08563383066793	22.37416268488431
34	22.865856556964047	28.351684707483976	26.764897696872442	22.01756103867954
35	23.55244069156572	27.277506621515506	27.24860582160981	21.921446865308962
36	23.87776482734624	29.346195456870312	25.796815968452496	20.979223747330956
37	23.367912886904705	28.62300998028281	26.97880164354286	21.030275489269627
38	22.747971715243462	29.091507157701397	26.844660101875316	21.31586102517982
39	23.472107876038383	27.93300338253441	26.68389940239991	21.9109893390273
40	23.86854319053423	27.48256920505688	27.134618785139665	21.514268819269226
41	22.856634920152032	27.111707295740743	27.11009113258812	22.9215666515191
42	23.12016458244999	27.56508859426129	27.46830894194552	21.846437881343203
43	23.0361240985137	27.326181652935617	27.59864774678336	22.039046501767324
44	23.200307261135837	27.699990683294768	27.07757773269422	22.02212432287517
45	22.990491256557345	27.904482856311684	26.614309318416463	22.490716568714504
46	23.358406044830467	28.162593618627323	26.324635840414345	22.15436449612786
47	22.859582041195047	27.66766742024235	27.8655048038073	21.607245734755303
48	23.15343852970983	27.938042008833758	26.955034538357253	21.953484923099154
49	22.627329889321345	27.88062068270534	27.585052962617198	21.906996465356116
50	22.43576702152539	27.091647858964095	28.16420978177995	22.308375337730567
51	22.341173942886698	27.18937819548729	26.700536376029827	23.768911485596185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1912.0
1	2018.0
2	2124.0
3	3826.5
4	5529.0
5	4003.0
6	2477.0
7	2445.5
8	2414.0
9	2502.5
10	2591.0
11	2646.5
12	2702.0
13	2794.0
14	2886.0
15	2757.5
16	2629.0
17	2744.0
18	2859.0
19	2992.0
20	3125.0
21	3686.5
22	4248.0
23	4542.5
24	4837.0
25	5599.0
26	7931.5
27	9502.0
28	11707.0
29	13912.0
30	16116.5
31	18321.0
32	21237.5
33	24154.0
34	28004.0
35	31854.0
36	34826.5
37	37799.0
38	41284.0
39	44769.0
40	48827.0
41	52885.0
42	57179.0
43	61473.0
44	65442.0
45	69411.0
46	75069.0
47	80727.0
48	83269.0
49	85811.0
50	85180.5
51	84550.0
52	79569.0
53	74588.0
54	70727.0
55	66866.0
56	61294.0
57	55722.0
58	51349.0
59	46976.0
60	41527.0
61	36078.0
62	32683.5
63	29289.0
64	26116.0
65	22943.0
66	19561.5
67	16180.0
68	14115.0
69	12050.0
70	10558.0
71	9066.0
72	8252.5
73	7439.0
74	6280.5
75	4483.5
76	3845.0
77	2799.0
78	1753.0
79	1375.0
80	997.0
81	704.0
82	411.0
83	355.5
84	300.0
85	220.0
86	140.0
87	151.0
88	162.0
89	101.5
90	41.0
91	32.5
92	24.0
93	18.5
94	13.0
95	8.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1051874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.052718423148935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29065035867845	19.09420676844109
2	8.75181510734135	4.560171493660503
3	3.403254788051372	2.659921161460075
4	1.9363473739248356	2.017884516090705
5	1.3285909016259136	1.730670232980875
6	0.9952462138770342	1.555732162310606
7	0.8043737121563064	1.466928528085391
8	0.7016939230387077	1.4624827356929753
9	0.6077227349803045	1.4249546364409056
>10	6.9921298100645926	41.165687419986455
>50	1.1054842740659196	18.83105814130419
>100	0.0790156554309094	3.0353892679739163
>500	0.00257260273495965	0.484386925325823
>1k	0.0011025440292684215	0.5105260102465484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2713	0.25792062547415373	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1577	0.1499228995107779	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.506842074240831E-5	0.0	0.0	0.0308972367412827	0.0
2	9.506842074240831E-5	0.0	0.0	0.1760667152149402	0.0
3	9.506842074240831E-5	0.0	0.0	0.2546882991689119	0.0
4	9.506842074240831E-5	0.0	0.0	0.3947240829224793	0.0
5	9.506842074240831E-5	0.0	0.0	0.8100780131460612	0.0
6	9.506842074240831E-5	0.0	0.0	1.1614508962100023	0.0
7	9.506842074240831E-5	0.0	0.0	1.4287832953376545	0.0
8	9.506842074240831E-5	0.0	0.0	1.8679043307468386	0.0
9	9.506842074240831E-5	0.0	0.0	2.0730619827089556	0.0
10	9.506842074240831E-5	0.0	0.0	2.6076317125435176	0.0
11	9.506842074240831E-5	0.0	0.0	3.1261348792726125	0.0
12	9.506842074240831E-5	0.0	0.0	3.5872167198732927	0.0
13	9.506842074240831E-5	0.0	0.0	3.7750719192602915	0.0
14	9.506842074240831E-5	0.0	0.0	3.86262993476405	0.0
15	9.506842074240831E-5	0.0	0.0	3.99962352905386	0.0
16	9.506842074240831E-5	0.0	0.0	4.271424143956406	0.0
17	9.506842074240831E-5	0.0	0.0	4.616522511251348	0.0
18	9.506842074240831E-5	0.0	0.0	4.998507425794344	0.0
19	1.9013684148481663E-4	0.0	0.0	5.225625882947957	0.0
20	1.9013684148481663E-4	0.0	0.0	5.448656398009647	0.0
21	1.9013684148481663E-4	0.0	0.0	5.716749344503239	0.0
22	1.9013684148481663E-4	0.0	0.0	6.016975417207765	0.0
23	1.9013684148481663E-4	0.0	0.0	6.339732705628241	0.0
24	1.9013684148481663E-4	0.0	0.0	6.593660457431214	0.0
25	1.9013684148481663E-4	0.0	0.0	6.831426577707976	0.0
26	1.9013684148481663E-4	0.0	0.0	7.062632976953513	0.0
27	1.9013684148481663E-4	0.0	0.0	7.300399097230277	0.0
28	1.9013684148481663E-4	0.0	0.0	7.5398764490804036	0.0
29	2.852052622272249E-4	0.0	0.0	7.800839264018314	0.0
30	2.852052622272249E-4	0.0	0.0	8.118557926139443	0.0
31	3.8027368296963325E-4	0.0	0.0	8.39910483575029	0.0
32	4.753421037120416E-4	0.0	0.0	8.67822571905	0.0
33	4.753421037120416E-4	0.0	0.0	8.96951536020474	0.0
34	4.753421037120416E-4	0.0	0.0	9.25025240665707	0.0
35	4.753421037120416E-4	0.0	0.0	9.54192232149478	0.0
36	5.704105244544498E-4	0.0	0.0	9.83482812580214	0.0
37	5.704105244544498E-4	0.0	0.0	10.139142140598588	0.0
38	5.704105244544498E-4	0.0	0.0	10.450110944847006	0.0
39	5.704105244544498E-4	0.0	0.0	10.75223838596638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.033014E-4	45.0	1
CGTAAAT	20	7.033014E-4	45.0	30
CAATGCG	35	1.2117926E-7	45.0	1
ACGGGCT	230	0.0	40.108692	5
CGTATGG	135	0.0	40.0	2
CGAATGG	110	0.0	38.863636	2
TCGTTTA	35	6.2477848E-6	38.571426	38
TATAGCG	70	0.0	38.571426	1
TCGACTA	35	6.2477848E-6	38.571426	1
TCGACAG	35	6.2477848E-6	38.571426	1
TAGGGCG	450	0.0	38.0	5
CGTTTTT	2135	0.0	37.728336	1
CGTAAGG	150	0.0	37.5	2
TCGTTAG	30	1.1398561E-4	37.499996	1
CTCGTAT	30	1.1398561E-4	37.499996	9
TACGTAC	30	1.1398561E-4	37.499996	40
GTTACGG	110	0.0	36.81818	2
ATGCGAG	55	2.748493E-9	36.81818	1
TCGTTGA	130	0.0	36.346153	24
TAATCGT	155	0.0	36.290325	21
>>END_MODULE