##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314225
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97735062455247	33.0	31.0	34.0	30.0	34.0
2	32.112288964913674	33.0	31.0	34.0	30.0	34.0
3	32.077244013047974	33.0	31.0	34.0	30.0	34.0
4	35.74192378073037	37.0	35.0	37.0	33.0	37.0
5	35.643370196515235	37.0	35.0	37.0	33.0	37.0
6	35.603491128968095	37.0	35.0	37.0	33.0	37.0
7	35.829154268438224	37.0	35.0	37.0	35.0	37.0
8	35.728197947330735	37.0	35.0	37.0	35.0	37.0
9	37.470366775399796	39.0	37.0	39.0	35.0	39.0
10	36.97218871827512	39.0	37.0	39.0	33.0	39.0
11	36.89685416500915	39.0	37.0	39.0	33.0	39.0
12	36.68515235897844	39.0	35.0	39.0	33.0	39.0
13	36.606940886307584	39.0	35.0	39.0	33.0	39.0
14	37.68316333837218	40.0	36.0	41.0	33.0	41.0
15	37.85276155620972	40.0	36.0	41.0	33.0	41.0
16	37.91261357307662	40.0	36.0	41.0	33.0	41.0
17	37.809572758373776	40.0	36.0	41.0	33.0	41.0
18	37.73834354363911	39.0	36.0	41.0	33.0	41.0
19	37.626955207255946	39.0	36.0	41.0	33.0	41.0
20	37.53809531386745	39.0	35.0	41.0	33.0	41.0
21	37.40526374413239	39.0	35.0	41.0	33.0	41.0
22	37.36950592728141	39.0	35.0	41.0	33.0	41.0
23	37.2826318720662	39.0	35.0	41.0	32.0	41.0
24	37.23561460736733	39.0	35.0	41.0	32.0	41.0
25	37.083344737051476	39.0	35.0	41.0	32.0	41.0
26	37.02760124114886	39.0	35.0	41.0	32.0	41.0
27	37.01097302888058	39.0	35.0	41.0	32.0	41.0
28	36.90247434163418	39.0	35.0	41.0	32.0	41.0
29	36.83646431697032	39.0	35.0	41.0	31.0	41.0
30	36.64058238523351	39.0	35.0	41.0	31.0	41.0
31	36.52584931179887	39.0	35.0	41.0	31.0	41.0
32	36.34312355796006	38.0	35.0	41.0	30.0	41.0
33	36.21846765852494	38.0	35.0	41.0	30.0	41.0
34	36.04054737847084	38.0	35.0	41.0	30.0	41.0
35	35.88914949478877	38.0	35.0	40.0	29.0	41.0
36	35.729483650250614	38.0	35.0	40.0	29.0	41.0
37	35.65536796881216	38.0	35.0	40.0	28.0	41.0
38	35.48786060943591	38.0	34.0	40.0	27.0	41.0
39	35.40552152120296	38.0	34.0	40.0	27.0	41.0
40	35.2209976927361	38.0	34.0	40.0	26.0	41.0
41	35.09265653592171	38.0	34.0	40.0	26.0	41.0
42	34.91796642533217	37.0	34.0	40.0	25.0	41.0
43	34.86718752486276	37.0	34.0	40.0	25.0	41.0
44	34.73251650887103	37.0	34.0	40.0	24.0	41.0
45	34.66216246320312	37.0	34.0	40.0	24.0	41.0
46	34.50243297000557	37.0	34.0	40.0	23.0	41.0
47	34.39842151324688	36.0	33.0	40.0	23.0	41.0
48	34.29719150290397	36.0	33.0	40.0	23.0	41.0
49	34.14829501153632	36.0	33.0	40.0	23.0	41.0
50	34.02266528761238	36.0	33.0	40.0	23.0	41.0
51	32.624950274484846	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	4.0
13	12.0
14	17.0
15	21.0
16	63.0
17	111.0
18	201.0
19	373.0
20	589.0
21	921.0
22	1294.0
23	1891.0
24	2509.0
25	3165.0
26	3788.0
27	4386.0
28	4944.0
29	5631.0
30	6809.0
31	8232.0
32	10506.0
33	14255.0
34	24739.0
35	30250.0
36	26068.0
37	35733.0
38	55698.0
39	71987.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.62041530750259	20.189354761715332	25.047975177022835	16.14225475375925
2	30.693611265812713	26.47370514758533	24.67499403293818	18.157689553663776
3	30.917336303604102	26.231203755270904	26.506484207176385	16.3449757339486
4	27.702442517304483	27.447847879704035	24.96014002705068	19.889569575940808
5	24.431537910732757	31.235579600604662	24.979234624870713	19.35364786379187
6	24.060466226430105	36.325881136128565	24.54387779457395	15.069774842867373
7	80.49964197629087	8.025459463760045	7.49144721139311	3.9834513485559713
8	82.08926724480867	7.06277349033336	7.242899196435674	3.605060068422309
9	77.4842867372106	8.792744052828388	9.024743416341794	4.698225793619222
10	43.05004375845334	25.93141856949638	15.462168828069059	15.556368843981225
11	33.941284111703396	26.465112578566313	21.381175908982417	18.21242740074787
12	29.048929906913834	23.563688439812235	27.443392473546023	19.943989179727904
13	23.304320152756784	27.192298512212588	28.55374333678097	20.949637998249663
14	20.73609674596229	29.364627257538388	28.489458190786856	21.409817805712468
15	20.93659002307264	26.868645079163024	31.203437027607606	20.991327870156734
16	23.802052669265652	24.059193253242103	30.934839684939135	21.20391439255311
17	22.582862598456522	25.786936112658125	27.107009308616437	24.523191980268916
18	24.437266290078767	25.023788686450793	29.086482615959902	21.45246240751054
19	25.427321187047497	27.78359455803962	25.30893468056329	21.480149574349593
20	25.924735460259367	26.1704192855438	25.988702362956477	21.916142891240355
21	25.86172328745326	27.343464078287848	25.274246161190227	21.520566473068662
22	23.997135810326995	25.53775161110669	26.13477603627973	24.33033654228658
23	23.34823772774286	28.05123717081709	25.460100246638557	23.140424854801495
24	23.30272893627178	26.653512610390646	28.634895377516113	21.408863075821465
25	24.227543957355397	27.289044474500756	25.60617391996181	22.877237648182035
26	21.46264619301456	28.581112260323017	26.768080197310844	23.18816134935158
27	21.550799586283713	27.027130241069298	27.259766091176708	24.162304081470285
28	21.998567905163497	27.442437743655024	27.58373776752327	22.975256583658208
29	22.02816453178455	26.884239000716047	27.468533694009068	23.619062773490334
30	23.715490492481504	27.23876203357467	26.602593682870555	22.443153791073275
31	25.573076617073752	27.3122762351818	24.639350783674118	22.47529636407033
32	24.938817726151644	27.580237091256265	24.842708250457473	22.638236932134618
33	25.574667833558756	27.240671493356672	23.797915506404646	23.386745166679926
34	22.665287612379664	27.33296204948683	25.81939692895218	24.18235340918132
35	23.3717877317209	27.438300580794017	25.241467101599174	23.94844458588591
36	25.29111305593126	28.186808815339326	24.235500039780412	22.286578088949003
37	24.043281088392078	28.536558198742938	25.312753600127298	22.107407112737686
38	23.613652637441323	27.937942557084895	26.135412522873736	22.312992282600046
39	24.866576497732517	26.915745087119102	25.605537433367807	22.61214098178057
40	24.6294852414671	26.182512530829822	26.665605855676667	22.522396372026414
41	21.698146232794972	27.26835866019572	25.891001670777307	25.142493436231998
42	21.980109793937466	28.021640544196035	26.345453098894104	23.65279656297239
43	22.408147028403214	26.078128729413635	26.620415307502586	24.893308934680565
44	23.155064046463522	26.237886864507914	26.615323414750574	23.991725674277987
45	23.96053783117193	27.024266051396296	25.09730288805792	23.917893229373856
46	23.827830376322698	27.328188400031824	25.976927360967462	22.867053862678016
47	23.481581669186095	26.92529238602912	26.340997692736096	23.25212825204869
48	24.300421672368525	25.66186649693691	26.603230169464553	23.43448166123001
49	23.305274882647783	26.22038348317289	27.883204710000797	22.591136924178535
50	22.323494311401067	26.174874691701806	27.751770228339566	23.74986076855756
51	23.303047179568782	27.056090381096347	25.299069138356273	24.341793300978598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2022.0
1	2062.0
2	2102.0
3	2227.5
4	2353.0
5	1751.5
6	1150.0
7	1191.0
8	1232.0
9	1391.0
10	1550.0
11	1476.5
12	1403.0
13	1352.0
14	1301.0
15	1422.5
16	1544.0
17	1585.5
18	1627.0
19	1516.0
20	1405.0
21	1462.5
22	1520.0
23	1601.0
24	1682.0
25	1901.5
26	2679.0
27	3237.0
28	3356.0
29	3475.0
30	3820.5
31	4166.0
32	5132.0
33	6098.0
34	6742.5
35	7387.0
36	7480.5
37	7574.0
38	8363.0
39	9152.0
40	10773.0
41	12394.0
42	13119.5
43	13845.0
44	15654.5
45	17464.0
46	17823.0
47	18182.0
48	19772.5
49	21363.0
50	21515.5
51	21668.0
52	22005.0
53	22342.0
54	20636.5
55	18931.0
56	18599.5
57	18268.0
58	17345.5
59	16423.0
60	15682.5
61	14942.0
62	14065.5
63	13189.0
64	11750.0
65	10311.0
66	9562.5
67	8814.0
68	7460.0
69	6106.0
70	5327.5
71	4549.0
72	4262.0
73	3975.0
74	3450.0
75	2257.5
76	1590.0
77	1454.0
78	1318.0
79	1094.0
80	870.0
81	580.0
82	290.0
83	237.5
84	185.0
85	127.5
86	70.0
87	68.5
88	67.0
89	44.5
90	22.0
91	17.0
92	12.0
93	9.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.49279974540536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29584249282256	20.212904765693374
2	7.232693070020542	3.8322857824807066
3	2.7688685478155373	2.200652398758851
4	1.547202902206686	1.6395894661468693
5	1.1243648419762875	1.4893786299626064
6	0.8444748759715065	1.3423502267483491
7	0.7255516715317069	1.3455326597183548
8	0.5994210001561617	1.2704272416262232
9	0.5153338859057984	1.2287373697191504
>10	7.465734500942976	47.957037154904924
>50	0.8180474972071066	13.74301853767205
>100	0.058860979975254364	2.588909221099531
>500	0.0024024889785818106	0.5890683427480309
>1k	0.0012012444892909053	0.5601082027209802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1760	0.5601082027209802	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	981	0.31219667435754633	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	870	0.2768716683904845	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	440	0.14002705068024504	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	412	0.13111623836422948	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	368	0.11711353329620494	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	351	0.11170339724719548	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.547298910016707E-4	0.0	0.0	0.03627973585806349	0.0
2	9.547298910016707E-4	0.0	0.0	0.2224520646033893	0.0
3	9.547298910016707E-4	0.0	0.0	0.31378789084254916	0.0
4	9.547298910016707E-4	0.0	0.0	0.4439493993157769	0.0
5	9.547298910016707E-4	0.0	0.0	0.8471636566154825	0.0
6	0.0012729731880022277	0.0	0.0	1.172090062853051	0.0
7	0.0012729731880022277	0.0	0.0	1.4460975415705306	0.0
8	0.0012729731880022277	0.0	0.0	1.8687246399872703	0.0
9	0.0012729731880022277	0.0	0.0	2.0647625109396133	0.0
10	0.0012729731880022277	0.0	0.0	2.4784787970403372	0.0
11	0.0012729731880022277	0.0	0.0	3.0780491685893865	0.0
12	0.0012729731880022277	0.0	0.0	3.509587079322142	0.0
13	0.0012729731880022277	0.0	0.0	3.663298591773411	0.0
14	0.0012729731880022277	0.0	0.0	3.7364945500835387	0.0
15	0.0012729731880022277	0.0	0.0	3.8437425411727264	0.0
16	0.0012729731880022277	0.0	0.0	4.083379743814146	0.0
17	0.0012729731880022277	0.0	0.0	4.3529318163736175	0.0
18	0.0012729731880022277	0.0	0.0	4.705545389450235	0.0
19	0.0012729731880022277	0.0	0.0	4.891717718195561	0.0
20	0.0012729731880022277	0.0	0.0	5.069615721218872	0.0
21	0.0012729731880022277	0.0	0.0	5.265971835468215	0.0
22	0.0012729731880022277	0.0	0.0	5.466146869281566	0.0
23	0.0012729731880022277	0.0	0.0	5.674596228816931	0.0
24	0.0012729731880022277	0.0	0.0	5.842946932930225	0.0
25	0.0012729731880022277	0.0	0.0	6.00525101440051	0.0
26	0.0012729731880022277	0.0	0.0	6.160553743336781	0.0
27	0.0012729731880022277	0.0	0.0	6.312992282600048	0.0
28	0.0012729731880022277	0.0	0.0	6.455565279656297	0.0
29	0.0012729731880022277	0.0	0.0	6.630917336303604	0.0
30	0.0012729731880022277	0.0	0.0	6.82281804439494	0.0
31	0.0012729731880022277	0.0	0.0	7.005171453576259	0.0
32	0.0012729731880022277	0.0	0.0	7.179250537035563	0.0
33	0.0012729731880022277	0.0	0.0	7.363195162701886	0.0
34	0.0012729731880022277	0.0	0.0	7.563688439812236	0.0
35	0.0012729731880022277	0.0	0.0	7.764181716922588	0.0
36	0.0012729731880022277	0.0	0.0	7.940488503460896	0.0
37	0.0012729731880022277	0.0	0.0	8.120932452860211	0.0
38	0.0012729731880022277	0.0	0.0	8.32588113612857	0.0
39	0.0012729731880022277	0.0	0.0	8.54260482138595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	30	2.16045E-6	45.000004	2
AACCTAG	30	2.16045E-6	45.000004	1
CAGCGCG	25	3.884315E-5	45.0	1
TTTACGA	25	3.884315E-5	45.0	16
TCCTGAT	20	7.025454E-4	45.0	38
GGACTAT	35	1.2081546E-7	45.0	8
ATATGCG	20	7.025454E-4	45.0	1
CCCGTAG	20	7.025454E-4	45.0	19
TACGCCC	20	7.025454E-4	45.0	19
ACGGCTA	20	7.025454E-4	45.0	15
GGACGTA	20	7.025454E-4	45.0	8
ACGCTAA	20	7.025454E-4	45.0	41
GCGATAC	35	1.2081546E-7	45.0	9
CTTTACG	25	3.884315E-5	45.0	15
ATCGGGC	20	7.025454E-4	45.0	4
CGACTCC	20	7.025454E-4	45.0	28
TGTACGC	20	7.025454E-4	45.0	17
ACGAGAC	40	6.7884685E-9	45.0	25
CCGTTTG	25	3.884315E-5	45.0	27
GCCCGTA	20	7.025454E-4	45.0	18
>>END_MODULE