##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671596
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945738211662963	33.0	31.0	34.0	30.0	34.0
2	32.04317625477221	33.0	31.0	34.0	30.0	34.0
3	32.021383391205426	33.0	31.0	34.0	30.0	34.0
4	35.7116436071686	37.0	35.0	37.0	33.0	37.0
5	35.59973853328489	37.0	35.0	37.0	33.0	37.0
6	35.55779814054878	37.0	35.0	37.0	33.0	37.0
7	35.80958195105391	37.0	35.0	37.0	35.0	37.0
8	35.675593660474455	37.0	35.0	37.0	35.0	37.0
9	37.39035223557019	39.0	37.0	39.0	35.0	39.0
10	36.93902733190787	39.0	37.0	39.0	33.0	39.0
11	36.86596406172759	39.0	37.0	39.0	33.0	39.0
12	36.7601176898016	39.0	35.0	39.0	33.0	39.0
13	36.69950982435869	39.0	35.0	39.0	32.0	39.0
14	37.78186439466584	40.0	37.0	41.0	33.0	41.0
15	37.898613154336836	40.0	37.0	41.0	33.0	41.0
16	37.980754799016076	40.0	37.0	41.0	33.0	41.0
17	37.84871410788629	40.0	36.0	41.0	33.0	41.0
18	37.863548919290764	40.0	36.0	41.0	33.0	41.0
19	37.76690450806735	40.0	36.0	41.0	33.0	41.0
20	37.59661165343451	40.0	36.0	41.0	33.0	41.0
21	37.520729724417656	39.0	35.0	41.0	32.0	41.0
22	37.516399740320075	39.0	35.0	41.0	33.0	41.0
23	37.350679575220816	39.0	35.0	41.0	32.0	41.0
24	37.323331288453176	39.0	35.0	41.0	32.0	41.0
25	37.215590920732105	39.0	35.0	41.0	32.0	41.0
26	37.135182460884224	39.0	35.0	41.0	32.0	41.0
27	37.04316583183938	39.0	35.0	41.0	31.0	41.0
28	36.93360889582428	39.0	35.0	41.0	31.0	41.0
29	36.87940517811303	39.0	35.0	41.0	31.0	41.0
30	36.73840523171668	39.0	35.0	41.0	31.0	41.0
31	36.690858790105956	39.0	35.0	41.0	31.0	41.0
32	36.453748980041574	39.0	35.0	41.0	30.0	41.0
33	36.3445940714358	39.0	35.0	41.0	30.0	41.0
34	36.138700945211106	39.0	35.0	41.0	30.0	41.0
35	36.04703869588264	39.0	35.0	41.0	29.0	41.0
36	35.82183783107702	38.0	35.0	40.0	28.0	41.0
37	35.71438186052329	38.0	35.0	40.0	28.0	41.0
38	35.61327047808504	38.0	35.0	40.0	27.0	41.0
39	35.450851404713546	38.0	35.0	40.0	27.0	41.0
40	35.366945306404446	38.0	34.0	40.0	26.0	41.0
41	35.23618812500372	38.0	34.0	40.0	26.0	41.0
42	35.09405505690921	38.0	34.0	40.0	26.0	41.0
43	34.98643083043973	38.0	34.0	40.0	25.0	41.0
44	34.92658235010334	37.0	34.0	40.0	25.0	41.0
45	34.84444517239531	37.0	34.0	40.0	24.0	41.0
46	34.635481747955616	37.0	34.0	40.0	24.0	41.0
47	34.52240632761362	37.0	33.0	40.0	23.0	41.0
48	34.44657353528014	37.0	33.0	40.0	23.0	41.0
49	34.322830987677115	36.0	33.0	40.0	24.0	41.0
50	34.21042561301735	36.0	33.0	40.0	23.0	41.0
51	32.677322378334594	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	6.0
12	6.0
13	15.0
14	35.0
15	73.0
16	109.0
17	200.0
18	376.0
19	762.0
20	1495.0
21	2607.0
22	3759.0
23	4717.0
24	5419.0
25	6365.0
26	7451.0
27	8527.0
28	9911.0
29	11143.0
30	13629.0
31	16998.0
32	21944.0
33	29387.0
34	48196.0
35	57373.0
36	59049.0
37	81258.0
38	122499.0
39	158225.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31816746972883	17.64513189477007	22.584857563177863	14.451843072323243
2	29.80929010893454	27.53604845770374	25.176594262026576	17.478067171335148
3	30.939880523409908	26.51683452551832	26.321330085348933	16.221954865722847
4	27.52398763542368	25.842470771118354	27.516393784358456	19.117147809099517
5	26.476185087463293	29.56896705757628	24.697139351634018	19.257708503326405
6	24.24791094646186	38.097308500944024	23.411098338882304	14.243682213711814
7	80.11334194962447	6.335207475923026	9.806937504094723	3.7445130703577743
8	82.0065932495131	7.937212252604245	6.660849677484679	3.3953448203979777
9	76.92258441086605	7.1800606316892885	7.929022805377042	7.968332152067611
10	43.721225260424426	24.93463332122288	15.49562534619027	15.84851607216243
11	33.24275308369913	26.040357595935653	20.952030685114266	19.764858635250953
12	27.698944008004812	22.39218220477787	27.64295796877885	22.26591581843847
13	26.604833858450615	23.688199453242724	29.111995902298403	20.594970786008254
14	22.47913924442671	28.445672696085143	26.6081096373415	22.467078422146646
15	21.09259137934115	25.180614536119926	30.521176421539142	23.20561766299978
16	24.382217880987973	23.052549449371348	27.938075867039114	24.62715680260156
17	23.409758247517853	24.93463332122288	26.880297083365594	24.775311347893673
18	24.14874418549247	23.921524249697733	27.470681778926615	24.45904978588318
19	25.02724852441051	25.707717139470752	24.94937432623184	24.315660009886898
20	28.058386291758737	24.25267571575769	25.551224247910948	22.13771374457263
21	26.107808861279697	27.368983734268816	24.869862238607734	21.653345165843753
22	25.2693583642547	23.569229119887552	26.14935169357769	25.012060822280063
23	24.86226838754251	27.055402354987223	24.661701379996305	23.420627877473958
24	23.460235022245516	26.41498758182002	26.383718783316162	23.7410586126183
25	24.613458090876062	27.52919910184099	25.56566745483892	22.29167535244403
26	22.855853816878007	26.084282812881554	26.505369299400233	24.554494070840207
27	24.834424266970025	25.137284915336007	26.169453064044458	23.858837753649514
28	23.21663619199638	26.628211007808268	27.708026849474983	22.447125950720373
29	24.0902268625781	25.09008391949922	27.590545506524755	23.229143711397924
30	23.81506143574411	28.110054258810358	25.741517221663024	22.333367083782512
31	26.209358006897006	25.785144640527935	23.49016372938493	24.515333623190134
32	26.836818563541176	26.045122365231478	24.21798223932245	22.900076831904894
33	26.83905204914859	25.37641677437031	23.2738134235463	24.5107177529348
34	24.53215921476602	25.654857980095176	25.351997331729194	24.46098547340961
35	26.178982602636108	24.180161883036828	25.028439716734464	24.6124157975926
36	26.797807014931596	27.13491444261133	24.707413385428147	21.359865157028928
37	25.273378638348053	26.52532177082651	26.185683059458363	22.015616531367073
38	25.771594827842932	26.672285123794666	24.48138464195737	23.074735406405043
39	25.68880696132794	25.46813858331497	25.037671457245132	23.80538299811196
40	25.295862393462738	24.614500384159523	26.350365398245373	23.739271824132366
41	23.11032227708325	24.56208792190543	27.002096498490165	25.325493302521156
42	23.3479651457126	25.422277678842637	26.606769545977045	24.622987629467715
43	24.049875222604065	23.840225373587693	27.116599860630497	24.993299543177745
44	24.894281681248845	24.500443719140673	26.271895603904728	24.33337899570575
45	25.21754149816259	24.015330645209325	25.65992054747199	25.1072073091561
46	25.123735102650997	25.84827783369764	25.90753965181448	23.120447411836878
47	24.301514601039912	26.107213265117718	26.906503314492642	22.684768819349728
48	24.11628419466465	25.810308578371522	25.682999898748655	24.390407328215176
49	23.775603190013044	25.663940821565344	27.63164164170126	22.92881434672035
50	24.2660766294022	24.579806907724286	27.287387060077783	23.866729402795727
51	24.07891053550051	24.831446286160133	25.987200638479084	25.102442539860277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1599.0
1	1962.5
2	2326.0
3	3075.0
4	3824.0
5	2812.0
6	1800.0
7	1795.5
8	1791.0
9	1869.0
10	1947.0
11	1996.5
12	2046.0
13	2068.0
14	2090.0
15	1998.5
16	1907.0
17	1985.5
18	2064.0
19	2010.0
20	1956.0
21	1993.0
22	2030.0
23	2210.5
24	2391.0
25	3211.0
26	4528.0
27	5025.0
28	6207.0
29	7389.0
30	9047.5
31	10706.0
32	11715.5
33	12725.0
34	14067.5
35	15410.0
36	16295.0
37	17180.0
38	18268.0
39	19356.0
40	21870.0
41	24384.0
42	26155.5
43	27927.0
44	30498.5
45	33070.0
46	35269.0
47	37468.0
48	45279.0
49	53090.0
50	50178.0
51	47266.0
52	47616.0
53	47966.0
54	44866.0
55	41766.0
56	41327.5
57	40889.0
58	37602.5
59	34316.0
60	33285.5
61	32255.0
62	30282.0
63	28309.0
64	26633.5
65	24958.0
66	22303.5
67	19649.0
68	17738.5
69	15828.0
70	14402.0
71	12976.0
72	11479.5
73	9983.0
74	8977.0
75	6301.0
76	4631.0
77	3800.5
78	2970.0
79	2543.0
80	2116.0
81	1586.0
82	1056.0
83	858.5
84	661.0
85	462.0
86	263.0
87	194.5
88	126.0
89	81.5
90	37.0
91	29.5
92	22.0
93	17.5
94	13.0
95	16.0
96	19.0
97	18.5
98	18.0
99	9.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.37304793980682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17648141680118	18.322799855176918
2	7.805364831437774	3.8048106244863003
3	2.8230497458514914	2.0641898037629356
4	1.5997153973573888	1.5595976027935499
5	1.138674262074808	1.3876481188686725
6	0.8446000869926387	1.2351287046141952
7	0.7100173337984945	1.211370056033517
8	0.6007332468064485	1.1713360178759493
9	0.512004262148069	1.1231193984048276
>10	7.617277074618273	46.18046518472233
>50	1.0991337026199648	16.704053854158953
>100	0.06375343283462151	2.5998858776048293
>500	0.0055171239953037835	0.9137924272990712
>1k	0.0036780826635358557	1.7218024741979225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	3269	0.486750963376792	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	2096	0.3120923888766461	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	1953	0.2907998260859207	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.2625090083919499	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1418	0.21113883942131878	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1025	0.15262151650694764	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	876	0.13043555947325475	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	845	0.1258196892179227	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	786	0.11703464582874229	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTT	713	0.10616501587263773	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	678	0.10095354945532731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4889904049458305E-4	0.0	0.0	0.03975604381205367	0.0
2	1.4889904049458305E-4	0.0	0.0	0.2711451527406357	0.0
3	1.4889904049458305E-4	0.0	0.0	0.34857265379781893	0.0
4	1.4889904049458305E-4	0.0	0.0	0.5186153580426328	0.0
5	1.4889904049458305E-4	0.0	0.0	1.1112335392110733	0.0
6	1.4889904049458305E-4	0.0	0.0	1.4236237261687086	0.0
7	1.4889904049458305E-4	0.0	0.0	1.7159125426595752	0.0
8	1.4889904049458305E-4	0.0	0.0	2.091138124705924	0.0
9	1.4889904049458305E-4	0.0	0.0	2.278006420526626	0.0
10	1.4889904049458305E-4	0.0	0.0	3.214432486197059	0.0
11	1.4889904049458305E-4	0.0	0.0	3.799754614381265	0.0
12	1.4889904049458305E-4	0.0	0.0	4.609616495631302	0.0
13	1.4889904049458305E-4	0.0	0.0	4.824924508186469	0.0
14	1.4889904049458305E-4	0.0	0.0	4.917837509455089	0.0
15	1.4889904049458305E-4	0.0	0.0	5.092198285874246	0.0
16	1.4889904049458305E-4	0.0	0.0	5.36036545780499	0.0
17	2.977980809891661E-4	0.0	0.0	5.673500139965098	0.0
18	2.977980809891661E-4	0.0	0.0	6.04396095271562	0.0
19	2.977980809891661E-4	0.0	0.0	6.348310591486548	0.0
20	2.977980809891661E-4	0.0	0.0	6.536221180590712	0.0
21	2.977980809891661E-4	0.0	0.0	6.756442861482201	0.0
22	2.977980809891661E-4	0.0	0.0	7.018802970833656	0.0
23	2.977980809891661E-4	0.0	0.0	7.23172859874091	0.0
24	2.977980809891661E-4	0.0	0.0	7.423361663857438	0.0
25	2.977980809891661E-4	0.0	0.0	7.594148863304725	0.0
26	4.4669712148374914E-4	0.0	0.0	7.747961572135629	0.0
27	4.4669712148374914E-4	0.0	0.0	7.927236016891107	0.0
28	5.955961619783322E-4	0.0	0.0	8.097278721135922	0.0
29	5.955961619783322E-4	0.0	0.0	8.268363718664197	0.0
30	5.955961619783322E-4	0.0	0.0	8.475184485911173	0.0
31	5.955961619783322E-4	0.0	0.0	8.63852673333373	0.0
32	7.444952024729152E-4	0.0	0.0	8.813334206874371	0.0
33	7.444952024729152E-4	0.0	0.0	8.995288834358751	0.0
34	7.444952024729152E-4	0.0	0.0	9.167565024210983	0.0
35	7.444952024729152E-4	0.0	0.0	9.365154050947297	0.0
36	7.444952024729152E-4	0.0	0.0	9.529836389734305	0.0
37	7.444952024729152E-4	0.0	0.0	9.703899368072472	0.0
38	7.444952024729152E-4	0.0	0.0	9.88704518788081	0.0
39	7.444952024729152E-4	0.0	0.0	10.071679998094092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGT	20	7.0311886E-4	45.000004	37
CATGCGG	70	0.0	45.000004	2
CTATCGA	20	7.0311886E-4	45.000004	2
CGAGGGT	75	0.0	45.000004	4
TGTTACG	20	7.0311886E-4	45.000004	31
ATAGTCG	20	7.0311886E-4	45.000004	1
CGTCATC	20	7.0311886E-4	45.000004	37
CGCATCG	45	3.8380676E-10	45.0	21
TAACGCC	45	3.8380676E-10	45.0	12
TCTAGCG	25	3.8890677E-5	45.0	1
CGTTCAT	25	3.8890677E-5	45.0	17
TATAGCG	25	3.8890677E-5	45.0	1
TATCGTC	25	3.8890677E-5	45.0	17
CTCCGTA	30	2.16415E-6	44.999996	43
GTAATCG	30	2.16415E-6	44.999996	18
TATCGAG	30	2.16415E-6	44.999996	1
TCGAATA	30	2.16415E-6	44.999996	44
CTCGAAT	85	0.0	42.35294	43
CGAATGC	165	0.0	42.272728	45
CATCGAA	150	0.0	42.000004	42
>>END_MODULE