FastQCFastQC Report
Sat 18 Jun 2016
SRR3553588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568050
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30110.5300589736818943No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT21920.3858815245136872No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT12840.22603644045418536No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGT12170.21424170407534546No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC11810.2079042337822375No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT7410.13044626353313968No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.12005985388610158No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6810.11988381304462636No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.11741924126397324No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT5930.1043922189948068No Hit
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5830.10263181058005456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT302.163555E-645.00000427
TCATACG302.163555E-645.0000041
TATCGCG302.163555E-645.0000041
ATTTACG207.03027E-445.01
TCGTTCA551.8189894E-1245.016
TCCGAAC207.03027E-445.043
CGCATCG207.03027E-445.021
ATTCGGT207.03027E-445.019
TTCGTAG253.8883056E-545.018
TTGTAAC207.03027E-445.041
GTCAGGT207.03027E-445.012
ATCCGGT253.8883056E-545.09
GTTACGT207.03027E-445.033
TACGCAA207.03027E-445.01
CGCTAAC253.8883056E-545.031
CGGTCTA207.03027E-445.031
CGACTAC253.8883056E-545.036
TTATTCG207.03027E-445.017
ACGACAG253.8883056E-545.01
CGGGTTA800.042.18756