##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568050
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.080809787870788	31.0	31.0	34.0	28.0	34.0
2	31.276311944371095	31.0	31.0	34.0	28.0	34.0
3	31.48689023853534	31.0	31.0	34.0	30.0	34.0
4	35.48374790951501	37.0	35.0	37.0	33.0	37.0
5	35.08540093301646	37.0	35.0	37.0	32.0	37.0
6	35.117509022093124	37.0	35.0	37.0	32.0	37.0
7	34.02395387729953	37.0	35.0	37.0	31.0	37.0
8	34.82535516239768	37.0	35.0	37.0	32.0	37.0
9	36.11461667106769	39.0	35.0	39.0	32.0	39.0
10	35.74834961711117	37.0	35.0	39.0	30.0	39.0
11	36.10897632250682	37.0	35.0	39.0	31.0	39.0
12	35.95739635595458	37.0	35.0	39.0	31.0	39.0
13	35.787369069624155	37.0	35.0	39.0	30.0	39.0
14	36.84359827479975	39.0	35.0	40.0	31.0	41.0
15	36.90557873426635	38.0	35.0	40.0	31.0	41.0
16	36.8955848956958	38.0	35.0	40.0	31.0	41.0
17	36.8428993926591	38.0	35.0	40.0	31.0	41.0
18	36.66660505237215	38.0	35.0	40.0	31.0	41.0
19	36.70068303846492	38.0	35.0	40.0	31.0	41.0
20	36.67056421089693	38.0	35.0	40.0	31.0	41.0
21	36.725670275503916	38.0	35.0	40.0	31.0	41.0
22	36.68622128333774	38.0	35.0	40.0	31.0	41.0
23	36.63572572836898	38.0	35.0	40.0	31.0	41.0
24	36.28937241440014	38.0	35.0	40.0	30.0	41.0
25	36.3831141624857	38.0	35.0	40.0	30.0	41.0
26	36.274745180881965	38.0	35.0	40.0	30.0	41.0
27	36.17858463163454	38.0	35.0	40.0	30.0	41.0
28	36.11188979843324	38.0	35.0	40.0	30.0	41.0
29	35.91001848428836	38.0	34.0	40.0	29.0	41.0
30	35.88174104392219	38.0	35.0	40.0	29.0	41.0
31	35.763675732770004	38.0	34.0	40.0	29.0	41.0
32	35.6137593521697	38.0	34.0	40.0	29.0	41.0
33	35.58850981427691	38.0	34.0	40.0	28.0	41.0
34	35.55609013291083	38.0	34.0	40.0	28.0	41.0
35	35.521073849132996	38.0	34.0	40.0	28.0	41.0
36	35.34373734706452	38.0	34.0	40.0	27.0	41.0
37	35.3010263181058	38.0	34.0	40.0	27.0	41.0
38	35.18628289763225	38.0	34.0	40.0	27.0	41.0
39	35.075697561834346	38.0	34.0	40.0	26.0	41.0
40	34.86654695889446	37.0	34.0	40.0	25.0	41.0
41	34.843293724144004	37.0	34.0	40.0	25.0	41.0
42	34.78242936361236	37.0	34.0	40.0	25.0	41.0
43	34.69651439133879	37.0	33.0	40.0	25.0	41.0
44	34.60496259132119	37.0	33.0	40.0	24.0	41.0
45	34.40075873602676	37.0	33.0	40.0	24.0	41.0
46	34.3402623008538	37.0	33.0	40.0	24.0	41.0
47	34.17954405422058	37.0	33.0	40.0	23.0	41.0
48	34.104313000616145	36.0	33.0	40.0	23.0	41.0
49	33.92391338790599	36.0	33.0	40.0	23.0	41.0
50	33.780105624504884	36.0	33.0	39.0	23.0	41.0
51	31.82100343279641	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	12.0
11	14.0
12	6.0
13	14.0
14	38.0
15	50.0
16	135.0
17	289.0
18	550.0
19	985.0
20	1629.0
21	2442.0
22	3358.0
23	4370.0
24	5035.0
25	5772.0
26	6829.0
27	8166.0
28	9682.0
29	11996.0
30	14897.0
31	18962.0
32	24788.0
33	33950.0
34	51433.0
35	55201.0
36	59759.0
37	81517.0
38	103920.0
39	62243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.543790159316963	31.68559105712525	19.935393011178594	25.835225772379193
2	25.198486048763314	30.01161869553736	20.01830824751342	24.7715870081859
3	20.634979315201125	32.44643957398116	19.790511398644487	27.128069712173225
4	21.101135463427514	33.313088636563684	18.068655928175335	27.517119971833466
5	18.06531115218731	35.64070064254907	17.118387465892088	29.175600739371532
6	19.144617551271896	39.2880908370742	19.070328316169352	22.49696329548455
7	81.25816389402341	10.329900536924566	4.408942874746941	4.002992694305079
8	85.18704339406742	6.461227004665082	4.010034327964088	4.341695273303406
9	78.30296628817885	10.191004313000617	6.911891558841651	4.594137839978875
10	40.42760320394331	29.92747117331221	12.584103512014789	17.06082211072969
11	30.96100695361324	29.463779596866473	21.570812428483407	18.00440102103688
12	29.656896399964793	26.168471085291788	26.271102895871838	17.903529618871577
13	25.35005721327348	30.535340198926153	24.938297685062935	19.176304902737435
14	20.95977466772291	33.377167502860665	27.22401197077722	18.439045858639204
15	19.23404629874131	30.90308951676789	29.437725552328143	20.42513863216266
16	20.81172432004225	28.336766129742102	29.851949652319338	20.99955989789631
17	20.30402253322771	26.888126045242494	27.266965936097176	25.540885485432618
18	22.776164070064254	28.119179649678728	27.022269166446616	22.0823871138104
19	22.952028870698	31.954933544582342	23.911979579262386	21.181058005457267
20	24.702050875803184	29.995422938121646	23.920077457970248	21.38244872810492
21	23.138632162661736	30.662617727312735	24.948508053868498	21.250242056157028
22	21.872546430771937	28.463163453921307	24.37179825719567	25.29249185811108
23	21.448640084499605	31.150955021565004	23.188451720799225	24.211953173136166
24	20.391514831440894	30.855382448728104	26.588152451368718	22.164950268462285
25	21.89437549511487	29.352873866737085	24.90203327171904	23.850717366429013
26	22.65768858375143	30.90168119003609	23.60320394331485	22.83742628289763
27	20.014963471525395	29.91233166094534	25.44547134935305	24.62723351817622
28	20.07552152099287	30.178505413255873	27.106592729513245	22.63938033623801
29	20.823519056421087	29.55268022181146	27.353754070944458	22.27004665082299
30	21.68752750638148	28.52319338086436	27.270486752926683	22.51879235982748
31	25.313792799929587	28.040665434380774	22.42108969280873	24.224452072880908
32	24.063198662089604	27.95458146289939	26.314408942874746	21.667810932136256
33	22.5534724055981	27.972009506205442	24.84305958982484	24.63145849837162
34	19.691752486576885	28.44362292051756	26.006161429451634	25.85846316345392
35	21.393363260276384	27.62186427251122	27.509374174808553	23.47539829240384
36	24.524073585071736	26.589032655576094	26.628993926590972	22.2578998327612
37	21.488249273831528	29.34266349793152	24.922806091013115	24.246281137223836
38	22.598010738491332	28.734090308951675	25.031599331044802	23.63629962151219
39	21.59281753366781	26.556641140744652	26.334477598802923	25.516063726784616
40	24.001408326731802	27.00308071472582	25.054308599595103	23.941202358947276
41	21.439485960742893	24.573188979843323	27.892967168383066	26.094357891030718
42	23.90212129213978	26.282545550567733	24.691488425314674	25.12384473197782
43	21.650558929671686	26.717542469853008	26.42496259132119	25.206936009154123
44	22.22445207288091	25.30305430859959	27.39670803626441	25.075785582255083
45	23.162397676260895	27.128069712173225	26.239415544406302	23.47011706715958
46	22.192060558049466	26.428659448992164	28.301910043130007	23.07736994982836
47	22.663497931520112	27.14092069360092	25.757591761288616	24.437989613590354
48	22.63603556024998	25.777132294692368	28.516855910571255	23.0699762344864
49	22.525129830120587	25.212041193556907	28.185899128597836	24.076929847724674
50	21.672035912331662	25.736114778628643	27.479975354282193	25.111873954757503
51	21.027726432532347	26.47372590440982	28.112842179385616	24.38570548367221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3652.0
1	2988.5
2	2325.0
3	1682.5
4	1040.0
5	1047.0
6	1054.0
7	1197.0
8	1340.0
9	1522.0
10	1704.0
11	1847.5
12	1991.0
13	2077.0
14	2163.0
15	2112.0
16	2061.0
17	1900.0
18	1739.0
19	1763.5
20	1788.0
21	1874.5
22	1961.0
23	2059.5
24	2158.0
25	2549.0
26	3913.5
27	4887.0
28	5129.0
29	5371.0
30	6158.0
31	6945.0
32	9103.5
33	11262.0
34	11692.0
35	12122.0
36	14854.5
37	17587.0
38	18734.5
39	19882.0
40	22769.0
41	25656.0
42	29258.5
43	32861.0
44	38190.5
45	43520.0
46	46378.0
47	49236.0
48	50380.0
49	51524.0
50	49853.5
51	48183.0
52	43850.5
53	39518.0
54	36186.5
55	32855.0
56	29929.5
57	27004.0
58	24889.0
59	22774.0
60	22076.5
61	21379.0
62	19215.5
63	17052.0
64	15248.5
65	13445.0
66	11749.5
67	10054.0
68	8735.0
69	7416.0
70	6615.0
71	5814.0
72	5071.0
73	4328.0
74	3919.5
75	2934.5
76	2358.0
77	1891.0
78	1424.0
79	1262.0
80	1100.0
81	794.5
82	489.0
83	372.0
84	255.0
85	198.5
86	142.0
87	119.5
88	97.0
89	72.5
90	48.0
91	33.5
92	19.0
93	14.5
94	10.0
95	7.5
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.870376685699142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33031299428967	25.282869577224247
2	8.65443285741013	5.516400703335048
3	2.937399021780955	2.808480399010896
4	1.322628924352046	1.6861072813800313
5	0.7854633712265469	1.2516506756904595
6	0.5259892469508327	1.005808525977016
7	0.4004509741804125	0.8933766373899451
8	0.3407740970905426	0.8688479071203684
9	0.26762871201436694	0.7676485077425734
>10	4.4201066103441535	35.497177331824794
>50	0.9582822230228062	19.4831511146843
>100	0.04994483522054713	2.598418173244228
>500	0.0038419104015805484	0.7859099866971604
>1k	0.0027442217154146774	1.5541531786788938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3011	0.5300589736818943	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	2192	0.3858815245136872	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	1284	0.22603644045418536	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGT	1217	0.21424170407534546	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	1181	0.2079042337822375	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	741	0.13044626353313968	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.12005985388610158	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	681	0.11988381304462636	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	667	0.11741924126397324	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT	593	0.1043922189948068	No Hit
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	583	0.10263181058005456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.802042073761113E-4	0.0	0.0	0.4904497843499692	0.0
2	8.802042073761113E-4	0.0	0.0	0.6372678461403045	0.0
3	8.802042073761113E-4	0.0	0.0	0.8543262036792536	0.0
4	8.802042073761113E-4	0.0	0.0	1.900184842883549	0.0
5	8.802042073761113E-4	0.0	0.0	2.099815157116451	0.0
6	8.802042073761113E-4	0.0	0.0	3.046386761728721	0.0
7	8.802042073761113E-4	0.0	0.0	3.7771322946923687	0.0
8	8.802042073761113E-4	0.0	0.0	4.286418449080187	0.0
9	0.0010562450488513335	0.0	0.0	5.117859343367662	0.0
10	0.0010562450488513335	0.0	0.0	6.081330868761553	0.0
11	0.0010562450488513335	0.0	0.0	7.181058005457266	0.0
12	0.0010562450488513335	0.0	0.0	7.6407006425490716	0.0
13	0.0010562450488513335	0.0	0.0	7.864448552064079	0.0
14	0.0010562450488513335	0.0	0.0	8.089604788310888	0.0
15	0.0010562450488513335	0.0	0.0	8.2571956693953	0.0
16	0.0010562450488513335	0.0	0.0	8.598538861015756	0.0
17	0.0010562450488513335	0.0	0.0	9.027726432532347	0.0
18	0.0010562450488513335	0.0	0.0	9.580142593081595	1.7604084147522224E-4
19	0.0010562450488513335	0.0	0.0	9.807587360267583	1.7604084147522224E-4
20	0.0010562450488513335	0.0	0.0	10.061790335357802	1.7604084147522224E-4
21	0.0010562450488513335	0.0	0.0	10.34856086612094	1.7604084147522224E-4
22	0.0010562450488513335	0.0	0.0	10.639204295396532	1.7604084147522224E-4
23	0.0012322858903265558	0.0	0.0	10.97825895607781	1.7604084147522224E-4
24	0.0012322858903265558	0.0	0.0	11.218026582167063	1.7604084147522224E-4
25	0.0012322858903265558	0.0	0.0	11.457794208256315	1.7604084147522224E-4
26	0.0012322858903265558	0.0	0.0	11.656368277440366	1.7604084147522224E-4
27	0.0012322858903265558	0.0	0.0	11.870257899832762	1.7604084147522224E-4
28	0.0012322858903265558	0.0	0.0	12.091541237567116	1.7604084147522224E-4
29	0.0012322858903265558	0.0	0.0	12.302262124812957	1.7604084147522224E-4
30	0.0012322858903265558	0.0	0.0	12.632514743420474	1.7604084147522224E-4
31	0.0012322858903265558	0.0	0.0	12.858375143033184	1.7604084147522224E-4
32	0.0012322858903265558	0.0	0.0	13.094621952292933	1.7604084147522224E-4
33	0.0012322858903265558	0.0	0.0	13.335445823431035	1.7604084147522224E-4
34	0.0012322858903265558	0.0	0.0	13.571692632690784	1.7604084147522224E-4
35	0.0012322858903265558	0.0	0.0	13.855822550831792	1.7604084147522224E-4
36	0.0012322858903265558	0.0	0.0	14.069712173224188	1.7604084147522224E-4
37	0.0012322858903265558	0.0	0.0	14.299445471349353	1.7604084147522224E-4
38	0.0012322858903265558	0.0	0.0	14.539213097438605	1.7604084147522224E-4
39	0.0012322858903265558	0.0	0.0	14.781621336149987	1.7604084147522224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAGT	30	2.163555E-6	45.000004	27
TCATACG	30	2.163555E-6	45.000004	1
TATCGCG	30	2.163555E-6	45.000004	1
ATTTACG	20	7.03027E-4	45.0	1
TCGTTCA	55	1.8189894E-12	45.0	16
TCCGAAC	20	7.03027E-4	45.0	43
CGCATCG	20	7.03027E-4	45.0	21
ATTCGGT	20	7.03027E-4	45.0	19
TTCGTAG	25	3.8883056E-5	45.0	18
TTGTAAC	20	7.03027E-4	45.0	41
GTCAGGT	20	7.03027E-4	45.0	12
ATCCGGT	25	3.8883056E-5	45.0	9
GTTACGT	20	7.03027E-4	45.0	33
TACGCAA	20	7.03027E-4	45.0	1
CGCTAAC	25	3.8883056E-5	45.0	31
CGGTCTA	20	7.03027E-4	45.0	31
CGACTAC	25	3.8883056E-5	45.0	36
TTATTCG	20	7.03027E-4	45.0	17
ACGACAG	25	3.8883056E-5	45.0	1
CGGGTTA	80	0.0	42.1875	6
>>END_MODULE