FastQCFastQC Report
Sat 18 Jun 2016
SRR3553587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663561
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36980.5572961641808365No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT22190.3344078389175976No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC13590.2048040798057752No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGT13220.19922810412305725No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT13210.1990774020775784No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8230.12402778342910448No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.12297286911075243No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7810.11769829751899223No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT7540.1136293422910629No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8890164E-545.040
TCGCACG207.031128E-445.01
CTATCCG253.8890164E-545.01
CTATACG207.031128E-445.01
TCGATAG253.8890164E-545.01
TCTAGCG207.031128E-445.01
TAGCCGT207.031128E-445.044
ACGATAT253.8890164E-545.035
CGTAGCA207.031128E-445.038
GAACGAC207.031128E-445.025
TCGACCA207.031128E-445.017
ACGACAA207.031128E-445.040
ATCGATA207.031128E-445.020
TACCGTT207.031128E-445.029
TAGTACG207.031128E-445.01
TGCGTAG207.031128E-445.01
ACGGGTA950.044.9999965
TACACCG302.16411E-644.9999961
ATAACGG1050.042.8571432
CGGGTTA700.041.7857136