##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663561
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09169315255116	31.0	31.0	34.0	28.0	34.0
2	31.2863052530212	31.0	31.0	34.0	28.0	34.0
3	31.50711690409774	31.0	31.0	34.0	30.0	34.0
4	35.49411583863428	37.0	35.0	37.0	33.0	37.0
5	35.13006340035053	37.0	35.0	37.0	32.0	37.0
6	35.143914425350495	37.0	35.0	37.0	32.0	37.0
7	32.73198394721812	36.0	35.0	37.0	26.0	37.0
8	34.22045900829012	37.0	35.0	37.0	29.0	37.0
9	34.421685421536225	38.0	35.0	39.0	28.0	39.0
10	34.9458075444458	37.0	35.0	39.0	27.0	39.0
11	35.83379222106181	37.0	35.0	39.0	31.0	39.0
12	35.91670095138201	37.0	35.0	39.0	31.0	39.0
13	35.837118215205535	37.0	35.0	39.0	31.0	39.0
14	36.9263579384563	39.0	36.0	40.0	31.0	41.0
15	36.99209567771463	39.0	35.0	40.0	32.0	41.0
16	36.99830761602927	39.0	35.0	40.0	32.0	41.0
17	36.95490844097227	38.0	35.0	40.0	32.0	41.0
18	36.82522028871498	38.0	35.0	40.0	31.0	41.0
19	36.81350621871991	39.0	35.0	40.0	31.0	41.0
20	36.74776697244112	38.0	35.0	40.0	31.0	41.0
21	36.8083130262327	39.0	35.0	40.0	31.0	41.0
22	36.78765629685892	39.0	35.0	40.0	31.0	41.0
23	36.73733839089398	38.0	35.0	40.0	31.0	41.0
24	36.41117847492544	38.0	35.0	40.0	30.0	41.0
25	36.49193216599529	38.0	35.0	40.0	31.0	41.0
26	36.4093564871956	38.0	35.0	40.0	30.0	41.0
27	36.31293882551868	38.0	35.0	40.0	30.0	41.0
28	36.25925875691911	38.0	35.0	40.0	30.0	41.0
29	35.9690804613291	38.0	35.0	40.0	30.0	41.0
30	35.973303132643416	38.0	35.0	40.0	30.0	41.0
31	35.887112111772694	38.0	35.0	40.0	29.0	41.0
32	35.81560549821343	38.0	34.0	40.0	29.0	41.0
33	35.77626623626163	38.0	34.0	40.0	29.0	41.0
34	35.71360281873106	38.0	34.0	40.0	29.0	41.0
35	35.63392815430684	38.0	34.0	40.0	29.0	41.0
36	35.485922168421595	38.0	34.0	40.0	28.0	41.0
37	35.41007382893208	38.0	34.0	40.0	27.0	41.0
38	35.292289631247165	38.0	34.0	40.0	27.0	41.0
39	35.24025221494331	38.0	34.0	40.0	27.0	41.0
40	35.04713507876443	38.0	34.0	40.0	26.0	41.0
41	34.9776704779214	38.0	34.0	40.0	26.0	41.0
42	34.915212015172685	38.0	34.0	40.0	26.0	41.0
43	34.819261529836744	37.0	34.0	40.0	26.0	41.0
44	34.69305610185047	37.0	33.0	40.0	25.0	41.0
45	34.510914595643804	37.0	33.0	40.0	24.0	41.0
46	34.45881087043995	37.0	33.0	40.0	24.0	41.0
47	34.31735590247166	37.0	33.0	40.0	24.0	41.0
48	34.25923163055092	37.0	33.0	40.0	23.0	41.0
49	34.07436241732109	36.0	33.0	40.0	23.0	41.0
50	33.87925149308052	36.0	33.0	40.0	23.0	41.0
51	32.00445173842345	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	13.0
11	10.0
12	13.0
13	20.0
14	33.0
15	73.0
16	151.0
17	302.0
18	613.0
19	1122.0
20	1713.0
21	2677.0
22	3730.0
23	4859.0
24	5752.0
25	6788.0
26	7814.0
27	9233.0
28	11041.0
29	13656.0
30	17385.0
31	22493.0
32	28878.0
33	39772.0
34	59709.0
35	66002.0
36	74022.0
37	101971.0
38	115539.0
39	68172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.47721008317246	31.440666344164292	20.034028521869125	26.048095050794124
2	25.321711191586004	29.97132140074537	19.94873116412809	24.758236243540534
3	20.550032325588756	32.48005835183201	19.77888995887341	27.191019363705827
4	21.11320586954327	33.313892769466555	18.11800271565086	27.454898645339316
5	18.20103954270971	35.55799692869231	17.13678169753798	29.104181831059993
6	19.15091453536299	39.31032715907053	18.999006873520294	22.539751432046188
7	78.18165925966113	13.797827177908287	4.222671314317749	3.7978422481128336
8	85.40752093628167	6.450047546495348	3.9797396170058215	4.162691900217162
9	75.22036406600147	13.841681473142636	6.638425103343927	4.299529357511969
10	40.515039310628566	29.957758216652273	12.52997086929461	16.997231603424552
11	30.988710909773175	29.50866612112526	21.52944491915589	17.97317804994567
12	29.684987514335532	26.25410474696373	26.26872284537518	17.792184893325558
13	25.432175790922013	30.589953297436107	24.87714015742336	19.100730754218528
14	21.030319744529894	33.36272023220171	27.19945867825264	18.407501345015756
15	19.29679411538653	30.831830080429683	29.589291715456454	20.282084088727338
16	20.759056062667938	28.354439154802648	29.84473168254313	21.041773099986287
17	20.266712480088493	27.10737972846505	27.234722956894693	25.391184834551762
18	22.87747471596432	28.157471581361776	27.04423557140941	21.920818131264497
19	23.067359293267685	31.890813354009655	23.975489819323318	21.06633753339934
20	24.77466276649773	30.015627802116157	23.805347209977683	21.404362221408434
21	23.18113933760423	30.613161412439847	25.079834408592426	21.12586484136349
22	21.88570455466792	28.525485976421155	24.41297785734846	25.175831611562465
23	21.455751618916725	31.25922108140774	23.2747253078466	24.010301991828936
24	20.313731518277898	30.853079068842206	26.723993724766824	22.109195688113076
25	21.769513277603718	29.491636789986153	25.064613501999062	23.67423643041107
26	22.703564555481712	31.152825437299658	23.526548425841785	22.617061581376845
27	19.997709328908723	30.006133573250988	25.396610108188998	24.59954698965129
28	20.1041049730168	30.305879941708447	27.032932917998497	22.557082167276256
29	20.84797026950047	29.56065832681547	27.352722658504643	22.238648745179418
30	21.80055789897236	28.503935583917677	27.213323266436696	22.482183250673263
31	25.413338035237153	28.159430707953	22.453700564077756	23.97353069273209
32	23.957104169774894	28.136071890903775	26.360198986980848	21.54662495234048
33	22.514584190451217	28.092217595669428	24.840670262417472	24.552527951461887
34	19.75372271727844	28.52247193551158	26.01147445374276	25.712330893467218
35	21.364576881402012	27.699488065151506	27.464694278295436	23.471240775151042
36	24.39835975893701	26.818936013418508	26.58414222656244	22.19856200108204
37	21.52899281301945	29.466017442254746	24.909239693110354	24.09575005161545
38	22.598073123646508	28.815888818058927	24.99046809562346	23.595569962671103
39	21.446106688006076	26.62377686452338	26.362158113572075	25.567958333898467
40	23.908126004994266	27.11446272460256	25.174927399289594	23.802483871113584
41	21.571490789844493	24.609794728743854	27.857574510858836	25.96113997055282
42	23.880396828626154	26.47834939063628	24.590504866922558	25.050748913815006
43	21.620167550534163	26.967829634351624	26.400587135169186	25.011415679945024
44	22.27813268109488	25.424489986602588	27.337652453956757	24.959724878345774
45	23.128393621686627	27.065785963912887	26.280326902877054	23.525493511523432
46	22.18002564948814	26.494775913593475	28.232822604101205	23.09237583281718
47	22.696632261389684	27.251752288033803	25.86438925735539	24.18722619322112
48	22.547587938411088	25.912915315999584	28.441997043225868	23.09749970236346
49	22.422053134527197	25.279363916806442	28.1672672143179	24.131315734348462
50	21.478055521647597	25.895132474633076	27.52316667194124	25.10364533177809
51	20.942611154061193	26.65331446543724	28.09899918771597	24.30507519278559
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4489.0
1	3619.5
2	2750.0
3	2002.0
4	1254.0
5	1291.0
6	1328.0
7	1481.0
8	1634.0
9	1793.0
10	1952.0
11	2145.5
12	2339.0
13	2378.5
14	2418.0
15	2410.5
16	2403.0
17	2229.0
18	2055.0
19	2082.5
20	2110.0
21	2121.5
22	2133.0
23	2359.5
24	2586.0
25	3086.0
26	4677.5
27	5769.0
28	6103.5
29	6438.0
30	7588.5
31	8739.0
32	11024.0
33	13309.0
34	14105.0
35	14901.0
36	18049.0
37	21197.0
38	22506.0
39	23815.0
40	27184.5
41	30554.0
42	35015.0
43	39476.0
44	45104.5
45	50733.0
46	54146.0
47	57559.0
48	58980.0
49	60401.0
50	57898.0
51	55395.0
52	50366.5
53	45338.0
54	41422.5
55	37507.0
56	34430.5
57	31354.0
58	28838.0
59	26322.0
60	25519.5
61	24717.0
62	22102.5
63	19488.0
64	17530.0
65	15572.0
66	13491.0
67	11410.0
68	10001.0
69	8592.0
70	7605.5
71	6619.0
72	5712.5
73	4806.0
74	4439.5
75	3300.0
76	2527.0
77	2039.5
78	1552.0
79	1372.5
80	1193.0
81	886.5
82	580.0
83	418.5
84	257.0
85	204.5
86	152.0
87	123.0
88	94.0
89	73.0
90	52.0
91	35.0
92	18.0
93	13.5
94	9.0
95	6.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	663561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.394645016244965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13638376957736	24.216693862827213
2	8.952430486265099	5.621167542978042
3	3.5318374644516113	3.3264235035459917
4	1.9512240666923784	2.450319476838444
5	1.190964996764858	1.8694961650103026
6	0.8221746033128039	1.54871278874265
7	0.5661872832961369	1.2442674140256036
8	0.425162004649691	1.0678248168297713
9	0.3164596263534445	0.8941623868205919
>10	4.023090950205753	31.483265963231727
>50	1.012300758332139	20.82617411800559
>100	0.0659240725396584	3.1734830449585623
>500	0.0034182852427971024	0.7573432967195797
>1k	0.0024416323162836442	1.520665619465893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3698	0.5572961641808365	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	2219	0.3344078389175976	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	1359	0.2048040798057752	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGT	1322	0.19922810412305725	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	1321	0.1990774020775784	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	823	0.12402778342910448	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	816	0.12297286911075243	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	781	0.11769829751899223	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	754	0.1136293422910629	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.535102273943165E-4	0.0	0.0	0.4382415482525344	0.0
2	7.535102273943165E-4	0.0	0.0	0.5705579441829765	0.0
3	7.535102273943165E-4	0.0	0.0	0.7646621787597523	0.0
4	7.535102273943165E-4	0.0	0.0	1.7101668120941405	0.0
5	7.535102273943165E-4	0.0	0.0	1.8884473318956359	0.0
6	7.535102273943165E-4	0.0	0.0	2.762519195673043	0.0
7	7.535102273943165E-4	0.0	0.0	3.433896808281379	0.0
8	7.535102273943165E-4	0.0	0.0	3.9242812642696	0.0
9	7.535102273943165E-4	0.0	0.0	4.700999606667661	0.0
10	7.535102273943165E-4	0.0	0.0	5.642284582728641	0.0
11	7.535102273943165E-4	0.0	0.0	6.740299686087639	0.0
12	7.535102273943165E-4	0.0	0.0	7.222094125483565	0.0
13	7.535102273943165E-4	0.0	0.0	7.446188067110635	0.0
14	7.535102273943165E-4	0.0	0.0	7.679474833511915	0.0
15	7.535102273943165E-4	0.0	0.0	7.839219001719511	0.0
16	7.535102273943165E-4	0.0	0.0	8.187039322684727	0.0
17	7.535102273943165E-4	0.0	0.0	8.608552943889107	0.0
18	7.535102273943165E-4	0.0	0.0	9.161328046705577	0.0
19	7.535102273943165E-4	0.0	0.0	9.394012004924942	0.0
20	7.535102273943165E-4	0.0	0.0	9.626093154962392	0.0
21	7.535102273943165E-4	0.0	0.0	9.901576494097755	0.0
22	9.042122728731797E-4	0.0	0.0	10.201172160509735	0.0
23	9.042122728731797E-4	0.0	0.0	10.54266299556484	0.0
24	0.001054914318352043	0.0	0.0	10.783484864240062	0.0
25	0.001054914318352043	0.0	0.0	11.022799712460497	0.0
26	0.001054914318352043	0.0	0.0	11.224891155447652	0.0
27	0.001054914318352043	0.0	0.0	11.435874019118062	0.0
28	0.001054914318352043	0.0	0.0	11.653939878925977	0.0
29	0.001054914318352043	0.0	0.0	11.872457844870329	0.0
30	0.001054914318352043	0.0	0.0	12.208222002197235	0.0
31	0.001054914318352043	0.0	0.0	12.43442577246101	0.0
32	0.001054914318352043	0.0	0.0	12.67569974727267	0.0
33	0.001054914318352043	0.0	0.0	12.922549697767048	0.0
34	0.001054914318352043	0.0	0.0	13.146643639394117	0.0
35	0.001054914318352043	0.0	0.0	13.417605917165114	0.0
36	0.001054914318352043	0.0	0.0	13.633561948336325	0.0
37	0.001054914318352043	0.0	0.0	13.860217824736536	0.0
38	0.001054914318352043	0.0	0.0	14.09425810136521	0.0
39	0.001054914318352043	0.0	0.0	14.341108051859587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8890164E-5	45.0	40
TCGCACG	20	7.031128E-4	45.0	1
CTATCCG	25	3.8890164E-5	45.0	1
CTATACG	20	7.031128E-4	45.0	1
TCGATAG	25	3.8890164E-5	45.0	1
TCTAGCG	20	7.031128E-4	45.0	1
TAGCCGT	20	7.031128E-4	45.0	44
ACGATAT	25	3.8890164E-5	45.0	35
CGTAGCA	20	7.031128E-4	45.0	38
GAACGAC	20	7.031128E-4	45.0	25
TCGACCA	20	7.031128E-4	45.0	17
ACGACAA	20	7.031128E-4	45.0	40
ATCGATA	20	7.031128E-4	45.0	20
TACCGTT	20	7.031128E-4	45.0	29
TAGTACG	20	7.031128E-4	45.0	1
TGCGTAG	20	7.031128E-4	45.0	1
ACGGGTA	95	0.0	44.999996	5
TACACCG	30	2.16411E-6	44.999996	1
ATAACGG	105	0.0	42.857143	2
CGGGTTA	70	0.0	41.785713	6
>>END_MODULE