SRR3553586_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348605
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2769	0.7943087448544914	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	2432	0.6976377275139485	Illumina PCR Primer Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	1244	0.3568508770671677	Illumina PCR Primer Index 10 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	968	0.27767817443811765	Illumina PCR Primer Index 10 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGT	815	0.23378895885027468	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC	813	0.23321524361383225	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	793	0.22747809124940835	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	644	0.18473630613445016	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	583	0.1672379914229572	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	510	0.1462973852928099	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	406	0.11646419299780554	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	392	0.1124481863427088	Illumina PCR Primer Index 10 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	370	0.1061373187418425	No Hit
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	351	0.10068702399563977	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	70	0.0	45.000004	2
TATCCGG	35	1.2086639E-7	45.000004	2
TTCTACG	20	7.0265174E-4	45.0	1
TCTGATC	25	3.8851955E-5	45.0	29
AATCCGG	25	3.8851955E-5	45.0	2
AGATCTA	20	7.0265174E-4	45.0	39
TCACCGT	25	3.8851955E-5	45.0	17
CAAGTCG	20	7.0265174E-4	45.0	19
CTATCCG	20	7.0265174E-4	45.0	1
TAGCGAT	25	3.8851955E-5	45.0	45
TAAACGG	25	3.8851955E-5	45.0	2
CGACCGG	20	7.0265174E-4	45.0	2
CACGATT	25	3.8851955E-5	45.0	36
CGATTAC	25	3.8851955E-5	45.0	38
TTTCCGT	20	7.0265174E-4	45.0	18
TATTGGG	45	3.8380676E-10	45.0	3
TAGCACG	20	7.0265174E-4	45.0	1
TACGAAG	55	1.8189894E-12	45.0	1
GGTATGC	25	3.8851955E-5	45.0	8
TATTACG	20	7.0265174E-4	45.0	1