SRR3553584_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450120
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2391	0.5311916822180752	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	2268	0.5038656358304452	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT	2246	0.4989780502976984	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	2236	0.4967564205100862	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1745	0.3876743979383276	TruSeq Adapter, Index 1 (95% over 24bp)
AACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	594	0.13196480938416422	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	560	0.12441126810628278	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	532	0.11819070470096864	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	525	0.11663556384964009	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	521	0.11574691193459521	RNA PCR Primer, Index 33 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	502	0.11152581533813205	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.10597174086910156	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1625474E-6	45.000004	6
ATGATCG	20	7.0287066E-4	45.000004	27
CGTATAG	40	6.7993824E-9	45.000004	1
CGAAATG	20	7.0287066E-4	45.000004	1
CGAAACG	20	7.0287066E-4	45.000004	1
ACCGCAT	20	7.0287066E-4	45.000004	23
TAAACGG	30	2.1625474E-6	45.000004	2
TACGGGT	65	0.0	45.000004	4
GCACCGA	30	2.1625474E-6	45.000004	9
CACTCGA	20	7.0287066E-4	45.000004	27
TGCGATC	20	7.0287066E-4	45.000004	25
ACTACCG	20	7.0287066E-4	45.000004	1
CGTACCG	20	7.0287066E-4	45.000004	1
CAACTCG	20	7.0287066E-4	45.000004	1
GATTCGC	20	7.0287066E-4	45.000004	32
ATTTGCG	40	6.7993824E-9	45.000004	12
CGATACG	20	7.0287066E-4	45.000004	1
ATACCCG	40	6.7993824E-9	45.000004	1
ACGACCG	30	2.1625474E-6	45.000004	2
AAATCCG	25	3.8870112E-5	45.0	1