##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450120
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07013240913534	31.0	31.0	34.0	28.0	34.0
2	31.2653692348707	31.0	31.0	34.0	28.0	34.0
3	31.482593530614057	31.0	31.0	34.0	30.0	34.0
4	35.46610237270061	37.0	35.0	37.0	33.0	37.0
5	35.059064249533456	37.0	35.0	37.0	32.0	37.0
6	35.11529369945792	36.0	35.0	37.0	32.0	37.0
7	34.03368657246956	37.0	35.0	37.0	31.0	37.0
8	34.82989202879232	37.0	35.0	37.0	32.0	37.0
9	36.16196125477651	39.0	35.0	39.0	32.0	39.0
10	35.75697369590331	37.0	35.0	39.0	30.0	39.0
11	36.12525770905536	37.0	35.0	39.0	31.0	39.0
12	35.91294988003199	37.0	35.0	39.0	31.0	39.0
13	35.781927041677775	37.0	35.0	39.0	30.0	39.0
14	36.8368901626233	39.0	35.0	40.0	31.0	41.0
15	36.88720563405314	38.0	35.0	40.0	31.0	41.0
16	36.822036345863324	38.0	35.0	40.0	31.0	41.0
17	36.739658313338666	38.0	35.0	40.0	31.0	41.0
18	36.57358704345508	38.0	35.0	40.0	31.0	41.0
19	36.6190460321692	38.0	35.0	40.0	31.0	41.0
20	36.64080689593886	38.0	35.0	40.0	31.0	41.0
21	36.6861548031636	38.0	35.0	40.0	31.0	41.0
22	36.69622767262064	38.0	35.0	40.0	31.0	41.0
23	36.60700702035013	38.0	35.0	40.0	31.0	41.0
24	36.308824313516396	38.0	35.0	40.0	30.0	41.0
25	36.42964764951569	38.0	35.0	40.0	31.0	41.0
26	36.333713232027016	38.0	35.0	40.0	30.0	41.0
27	36.238774104683195	38.0	35.0	40.0	30.0	41.0
28	36.16983026748423	38.0	35.0	40.0	30.0	41.0
29	35.97347374033591	38.0	34.0	40.0	30.0	41.0
30	35.93890962410024	38.0	34.0	40.0	30.0	41.0
31	35.85818004087799	38.0	34.0	40.0	29.0	41.0
32	35.723160490535854	38.0	34.0	40.0	29.0	41.0
33	35.69523682573536	38.0	34.0	40.0	29.0	41.0
34	35.64333066737759	38.0	34.0	40.0	29.0	41.0
35	35.59200879765396	38.0	34.0	40.0	29.0	41.0
36	35.47082555762908	38.0	34.0	40.0	28.0	41.0
37	35.458371101039724	38.0	34.0	40.0	28.0	41.0
38	35.30163511952368	38.0	34.0	40.0	28.0	41.0
39	35.20291255665156	38.0	34.0	40.0	27.0	41.0
40	34.96558695458989	37.0	34.0	40.0	26.0	41.0
41	35.00994179329957	37.0	34.0	40.0	26.0	41.0
42	34.96896161023727	37.0	34.0	40.0	26.0	41.0
43	34.92984537456678	37.0	34.0	40.0	26.0	41.0
44	34.82033235581623	37.0	34.0	40.0	26.0	41.0
45	34.578514618324	37.0	33.0	40.0	26.0	41.0
46	34.45345241268995	37.0	33.0	40.0	25.0	41.0
47	34.238700790900204	36.0	33.0	40.0	24.0	41.0
48	34.16423176041944	36.0	33.0	40.0	24.0	41.0
49	33.99353505731805	36.0	33.0	39.0	24.0	41.0
50	33.87278947836133	36.0	33.0	39.0	24.0	40.0
51	31.851128587932106	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	12.0
12	18.0
13	19.0
14	30.0
15	56.0
16	92.0
17	211.0
18	355.0
19	611.0
20	1001.0
21	1568.0
22	2168.0
23	2947.0
24	3665.0
25	4211.0
26	5009.0
27	6212.0
28	7295.0
29	9378.0
30	11826.0
31	15523.0
32	20260.0
33	27988.0
34	42731.0
35	44229.0
36	49705.0
37	66493.0
38	81976.0
39	44521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.55291922154092	32.316937705500756	19.378832311383633	26.751310761574693
2	25.63605260819337	31.60646049942238	18.71367635297254	24.043810539411712
3	20.62316715542522	31.641562250066652	20.20772238514174	27.527548209366394
4	21.14391717764152	33.1851506264996	18.94294854705412	26.727983648804766
5	18.158491069048253	36.02150537634409	17.649960010663822	28.170043543943834
6	19.262641073491512	38.03541277881454	19.723851417399803	22.978094730294142
7	81.01595130187505	10.221052163867412	4.759619656980361	4.00337687727717
8	84.97556207233626	5.691149026926153	3.894294854705412	5.438994046032169
9	79.33684350839776	9.448591486714655	6.711099262418911	4.503465742468675
10	38.53950057762374	33.05318581711544	12.246067715275927	16.161245889984894
11	32.085888207589086	27.723273793655025	22.18630587398916	18.00453212476673
12	28.21980805118635	26.594685861548033	26.62156758197814	18.563938505287478
13	24.610992624189105	31.268994934684084	24.144228205811782	19.97578423531503
14	19.464809384164223	32.14231760419444	29.01359637430019	19.379276637341153
15	19.94934684084244	29.50613169821381	29.07491335643828	21.46960810450547
16	22.158313338665245	27.78325779792055	29.19066026837288	20.867768595041323
17	20.651604016706656	27.74415711365858	26.821292099884474	24.78294676975029
18	23.880298587043455	27.491779969785835	25.995956633786545	22.631964809384165
19	22.990980183062295	30.739580556296097	24.21798631476051	22.0514529458811
20	26.223673687016795	30.68092952990314	22.930551852839244	20.164844930240825
21	23.0029769839154	30.94530347462899	25.10530525193282	20.946414289522792
22	23.07962321158802	27.585977072780594	24.548786990135966	24.785612725495422
23	21.972585088420864	32.13987381142807	22.33604372167422	23.551497378476853
24	20.420554518794987	30.332800142184308	27.637963209810717	21.60868212920999
25	22.027459344174886	28.972718386208125	25.274149115791346	23.725673153825646
26	24.994890251488492	29.730960632720166	21.740646938594153	23.533502177197192
27	21.21212121212121	28.37465564738292	25.0555407446903	25.357682395805565
28	20.598729227761485	30.3099173553719	26.90082644628099	22.190526970585623
29	22.88811872389585	30.91819959122012	23.749000266595573	22.444681418288457
30	23.67190971296543	26.85528303563494	27.180307473562603	22.292499777837023
31	27.936550253265796	27.428907846796406	22.21407624633431	22.420465653603483
32	26.319425930862884	26.460055096418735	24.707411356971473	22.51310761574691
33	23.965831333866525	26.64511685772683	23.409757398027192	25.979294410379456
34	21.792410912645515	27.12054563227584	27.916777748156047	23.1702657069226
35	21.901048609259753	26.980138629698747	28.868523949169113	22.25028881187239
36	24.687416688882966	29.25486536923487	24.30751799520128	21.750199946680883
37	22.579534346396514	27.759930685150625	23.980494090464767	25.68004087798809
38	22.757486892384254	30.333244468141828	24.84692970763352	22.062338931840397
39	22.812805474095796	26.070825557629078	25.21549808939838	25.90087087887674
40	24.538123167155426	26.679107793477296	25.343686128143606	23.439082911223675
41	20.407224740069317	24.30351906158358	30.051319648093845	25.237936550253266
42	24.49146894161557	26.083044521460945	25.159513018750555	24.26597351817293
43	22.842353150271038	25.251932817915225	25.60028436861281	26.305429663200925
44	23.716786634675195	24.720074646760864	25.516084599662314	26.047054118901624
45	25.65982404692082	26.179463254243313	25.18994934684084	22.970763351995025
46	22.996756420510085	25.128188038745225	27.819692526437397	24.055363014307297
47	22.365369234870702	27.903225806451616	25.42655291922154	24.304852039456147
48	22.365369234870702	25.97129654314405	29.38460854883142	22.278725673153826
49	24.38594152670399	24.608993157380255	27.641962143428415	23.36310317248734
50	22.005243046298766	25.96507597973874	26.05682928996712	25.97285168399538
51	21.001288545276815	24.297298498178264	28.64080689593886	26.060606060606062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2905.0
1	2342.5
2	1780.0
3	1309.0
4	838.0
5	839.0
6	840.0
7	890.0
8	940.0
9	1124.0
10	1308.0
11	1362.0
12	1416.0
13	1476.5
14	1537.0
15	1458.5
16	1380.0
17	1188.0
18	996.0
19	1056.0
20	1116.0
21	1238.0
22	1360.0
23	1346.5
24	1333.0
25	1697.0
26	2374.0
27	2687.0
28	3316.0
29	3945.0
30	4584.0
31	5223.0
32	6257.0
33	7291.0
34	8671.0
35	10051.0
36	10995.0
37	11939.0
38	12822.5
39	13706.0
40	15786.5
41	17867.0
42	21486.5
43	25106.0
44	30577.0
45	36048.0
46	38313.5
47	40579.0
48	40288.5
49	39998.0
50	39485.0
51	38972.0
52	36612.0
53	34252.0
54	31170.0
55	28088.0
56	25547.0
57	23006.0
58	21002.0
59	18998.0
60	18271.0
61	17544.0
62	16106.0
63	14668.0
64	13024.0
65	11380.0
66	9779.0
67	8178.0
68	7133.0
69	6088.0
70	5180.0
71	4272.0
72	4009.5
73	3747.0
74	3368.5
75	2265.5
76	1541.0
77	1256.0
78	971.0
79	739.0
80	507.0
81	386.0
82	265.0
83	227.0
84	189.0
85	135.5
86	82.0
87	63.5
88	45.0
89	40.5
90	36.0
91	34.0
92	32.0
93	22.5
94	13.0
95	9.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	450120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.10975968849434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57844520559131	26.8029188265824
2	8.343745876223348	5.692063334796882
3	2.7657467259201036	2.830168685411243
4	1.3638071872570525	1.8607654167511787
5	0.8915241202414875	1.5204836748966741
6	0.6570159777625226	1.3446394267788475
7	0.518659338457926	1.2383941776495317
8	0.4424560195703574	1.2073654800218114
9	0.446096107383039	1.3694607918727406
>10	5.642151204141094	44.237407983788195
>50	0.3042019427224664	6.360999158707601
>100	0.03900024906698287	2.4047193055190883
>500	0.003900024906698287	0.7170258375482881
>1k	0.003250020755581906	2.41358789967553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2391	0.5311916822180752	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	2268	0.5038656358304452	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT	2246	0.4989780502976984	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	2236	0.4967564205100862	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1745	0.3876743979383276	TruSeq Adapter, Index 1 (95% over 24bp)
AACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	594	0.13196480938416422	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	560	0.12441126810628278	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	532	0.11819070470096864	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	525	0.11663556384964009	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	521	0.11574691193459521	RNA PCR Primer, Index 33 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	502	0.11152581533813205	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.10597174086910156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4900915311472496	0.0
2	0.0	0.0	0.0	0.6378299120234604	0.0
3	0.0	0.0	0.0	0.9632986759086466	0.0
4	0.0	0.0	0.0	2.3424864480582954	0.0
5	0.0	0.0	0.0	2.550653159157558	0.0
6	0.0	0.0	0.0	3.687683284457478	0.0
7	0.0	0.0	0.0	4.52990313694126	0.0
8	0.0	0.0	0.0	5.134186439171776	0.0
9	0.0	0.0	0.0	6.375855327468231	0.0
10	0.0	0.0	0.0	7.453790100417667	0.0
11	0.0	0.0	0.0	8.973162712165644	0.0
12	0.0	0.0	0.0	9.521016617790812	0.0
13	2.2216297876121924E-4	0.0	0.0	9.818492846352084	0.0
14	2.2216297876121924E-4	0.0	0.0	10.139740513640806	0.0
15	2.2216297876121924E-4	0.0	0.0	10.3425753132498	0.0
16	2.2216297876121924E-4	0.0	0.0	10.724029147782813	0.0
17	2.2216297876121924E-4	0.0	0.0	11.177908113391984	0.0
18	2.2216297876121924E-4	0.0	0.0	11.777748156047275	0.0
19	2.2216297876121924E-4	0.0	0.0	12.063005420776681	0.0
20	4.443259575224385E-4	0.0	0.0	12.337154536568026	0.0
21	4.443259575224385E-4	0.0	0.0	12.657291388962943	0.0
22	4.443259575224385E-4	0.0	0.0	13.00830889540567	0.0
23	4.443259575224385E-4	0.0	0.0	13.363991824402381	0.0
24	4.443259575224385E-4	0.0	0.0	13.667466453390206	0.0
25	4.443259575224385E-4	0.0	0.0	13.918288456411624	0.0
26	4.443259575224385E-4	0.0	0.0	14.14889362836577	0.0
27	4.443259575224385E-4	0.0	0.0	14.392384253088066	0.0
28	4.443259575224385E-4	0.0	0.0	14.64120678930063	0.0
29	4.443259575224385E-4	0.0	0.0	14.912912112325602	0.0
30	4.443259575224385E-4	0.0	0.0	15.353683462187862	0.0
31	4.443259575224385E-4	0.0	0.0	15.614724962232293	0.0
32	4.443259575224385E-4	0.0	0.0	15.906869279303297	0.0
33	4.443259575224385E-4	0.0	0.0	16.199902248289344	0.0
34	4.443259575224385E-4	0.0	0.0	16.454501021949703	0.0
35	4.443259575224385E-4	0.0	0.0	16.795965520305696	0.0
36	4.443259575224385E-4	0.0	0.0	17.04989780502977	0.0
37	4.443259575224385E-4	0.0	0.0	17.31982582422465	0.0
38	4.443259575224385E-4	0.0	0.0	17.602861459166444	0.0
39	4.443259575224385E-4	0.0	0.0	17.925442104327736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1625474E-6	45.000004	6
ATGATCG	20	7.0287066E-4	45.000004	27
CGTATAG	40	6.7993824E-9	45.000004	1
CGAAATG	20	7.0287066E-4	45.000004	1
CGAAACG	20	7.0287066E-4	45.000004	1
ACCGCAT	20	7.0287066E-4	45.000004	23
TAAACGG	30	2.1625474E-6	45.000004	2
TACGGGT	65	0.0	45.000004	4
GCACCGA	30	2.1625474E-6	45.000004	9
CACTCGA	20	7.0287066E-4	45.000004	27
TGCGATC	20	7.0287066E-4	45.000004	25
ACTACCG	20	7.0287066E-4	45.000004	1
CGTACCG	20	7.0287066E-4	45.000004	1
CAACTCG	20	7.0287066E-4	45.000004	1
GATTCGC	20	7.0287066E-4	45.000004	32
ATTTGCG	40	6.7993824E-9	45.000004	12
CGATACG	20	7.0287066E-4	45.000004	1
ATACCCG	40	6.7993824E-9	45.000004	1
ACGACCG	30	2.1625474E-6	45.000004	2
AAATCCG	25	3.8870112E-5	45.0	1
>>END_MODULE