SRR3553583_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517004
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2781	0.5379068633898384	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT	2552	0.49361320221893834	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	2404	0.4649867312438588	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	2366	0.457636691398906	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1765	0.34139000858794133	TruSeq Adapter, Index 1 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	613	0.11856774802516035	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	605	0.11702037121569658	No Hit
AACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	577	0.11160455238257345	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	568	0.10986375347192671	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	554	0.10715584405536516	RNA PCR Primer, Index 33 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	544	0.10522162304353545	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	542	0.10483477884116951	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	40	6.8030204E-9	45.000004	1
CCGTAAG	20	7.02968E-4	45.000004	1
TATCTCG	20	7.02968E-4	45.000004	24
TATCTAG	20	7.02968E-4	45.000004	1
CGAATAA	20	7.02968E-4	45.000004	44
ACTAACG	20	7.02968E-4	45.000004	1
CGGCTAT	20	7.02968E-4	45.000004	39
GTCCCGT	20	7.02968E-4	45.000004	27
ATCGGGT	20	7.02968E-4	45.000004	4
TCGAATC	20	7.02968E-4	45.000004	20
ATCGAAT	20	7.02968E-4	45.000004	19
TGGTACG	20	7.02968E-4	45.000004	12
TAAGTCG	25	3.8878177E-5	45.0	36
CGACATT	25	3.8878177E-5	45.0	31
TACGGGT	25	3.8878177E-5	45.0	4
CGTTACG	45	3.8380676E-10	45.0	1
TAACCCG	25	3.8878177E-5	45.0	1
CCGCATA	25	3.8878177E-5	45.0	24
TAAACGG	30	2.1631768E-6	44.999996	2
ATGCGAG	30	2.1631768E-6	44.999996	1