##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517004
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09506502851042	31.0	31.0	34.0	28.0	34.0
2	31.287467795220152	31.0	31.0	34.0	28.0	34.0
3	31.50722044703716	31.0	31.0	34.0	30.0	34.0
4	35.48379122792087	37.0	35.0	37.0	33.0	37.0
5	35.10767808372856	37.0	35.0	37.0	32.0	37.0
6	35.1427242342419	37.0	35.0	37.0	32.0	37.0
7	32.68917261761998	36.0	35.0	37.0	26.0	37.0
8	34.18298697882415	37.0	35.0	37.0	28.0	37.0
9	34.40676087612475	38.0	35.0	39.0	28.0	39.0
10	34.92543771421498	37.0	35.0	39.0	27.0	39.0
11	35.832821409505534	37.0	35.0	39.0	31.0	39.0
12	35.87259286195078	37.0	35.0	39.0	31.0	39.0
13	35.84603020479532	37.0	35.0	39.0	31.0	39.0
14	36.92396577202498	39.0	35.0	40.0	31.0	41.0
15	36.974953772117814	39.0	35.0	40.0	32.0	41.0
16	36.94527315069129	38.0	35.0	40.0	32.0	41.0
17	36.867666401033645	38.0	35.0	40.0	31.0	41.0
18	36.73365970089206	38.0	35.0	40.0	31.0	41.0
19	36.74017802570193	38.0	35.0	40.0	31.0	41.0
20	36.73064811877664	38.0	35.0	40.0	31.0	41.0
21	36.79034011342272	38.0	35.0	40.0	31.0	41.0
22	36.81459331069005	39.0	35.0	40.0	31.0	41.0
23	36.73192663886547	38.0	35.0	40.0	31.0	41.0
24	36.44433118505853	38.0	35.0	40.0	30.0	41.0
25	36.545651484321205	38.0	35.0	40.0	31.0	41.0
26	36.47276810237445	38.0	35.0	40.0	31.0	41.0
27	36.389103372507755	38.0	35.0	40.0	30.0	41.0
28	36.32273638114986	38.0	35.0	40.0	30.0	41.0
29	36.03155101314496	38.0	35.0	40.0	30.0	41.0
30	36.034336291402	38.0	35.0	40.0	30.0	41.0
31	35.973590146304474	38.0	35.0	40.0	30.0	41.0
32	35.915211487725436	38.0	34.0	40.0	30.0	41.0
33	35.88278620668312	38.0	35.0	40.0	30.0	41.0
34	35.78925308121407	38.0	34.0	40.0	29.0	41.0
35	35.69751684706501	38.0	34.0	40.0	29.0	41.0
36	35.58462797193059	38.0	34.0	40.0	29.0	41.0
37	35.56169197917231	38.0	34.0	40.0	29.0	41.0
38	35.40995234079427	38.0	34.0	40.0	28.0	41.0
39	35.35144989207047	38.0	34.0	40.0	28.0	41.0
40	35.128786237630656	38.0	34.0	40.0	27.0	41.0
41	35.135304562440524	38.0	34.0	40.0	27.0	41.0
42	35.10203015837401	38.0	34.0	40.0	27.0	41.0
43	35.05628969988627	37.0	34.0	40.0	27.0	41.0
44	34.88937803189144	37.0	34.0	40.0	26.0	41.0
45	34.65343208176339	37.0	33.0	40.0	26.0	41.0
46	34.539196988804726	37.0	33.0	40.0	25.0	41.0
47	34.37399130374233	37.0	33.0	40.0	24.0	41.0
48	34.3286783080982	36.0	33.0	40.0	24.0	41.0
49	34.15517674911606	36.0	33.0	40.0	24.0	41.0
50	33.95576049701743	36.0	33.0	40.0	24.0	41.0
51	32.020450518758075	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	9.0
11	17.0
12	14.0
13	11.0
14	22.0
15	61.0
16	104.0
17	220.0
18	372.0
19	691.0
20	1163.0
21	1704.0
22	2461.0
23	3286.0
24	4005.0
25	4785.0
26	5544.0
27	6849.0
28	8526.0
29	10535.0
30	13334.0
31	17587.0
32	23360.0
33	32280.0
34	48650.0
35	52281.0
36	60196.0
37	81930.0
38	89159.0
39	47838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.608730299958996	32.08563183263573	19.432151395347038	26.873486472058243
2	25.628041562541103	31.41561767413792	18.748210845564056	24.208129917756924
3	20.674308129144066	31.424515090792333	20.18843181097245	27.712744969091148
4	20.977593983798965	33.3283688327363	18.88708791421343	26.8069492692513
5	18.245893648791885	35.97283580010986	17.522688412468764	28.25858213862949
6	19.20894229058189	38.02330349475053	19.69346465404523	23.074289560622354
7	77.78044270450519	13.847861912093522	4.57307873826895	3.7986166451323395
8	85.14305498603493	5.6929927041183435	3.8866237011705906	5.277328608676142
9	75.85105724520507	13.379587005129553	6.393760976704242	4.375594772961137
10	38.482487562958895	33.09471493450727	12.282690269320934	16.1401072332129
11	32.31058173631152	27.624544490951713	22.125360732218706	17.93951304051806
12	28.365737982684852	26.596119178961864	26.688768365428505	18.349374472924772
13	24.689170683398967	31.24115093887088	24.06596467338744	20.00371370434271
14	19.555554695901773	32.266868341444166	28.857610385993144	19.319966576660917
15	19.860774771568497	29.538262760056018	29.216601805788738	21.384360662586747
16	22.055535353691656	27.99630176942538	29.070568119395595	20.87759475748737
17	20.63407633209801	27.79185460847498	26.749309483098777	24.82475957632823
18	24.02476576583547	27.488762175921273	26.021848960549626	22.464623097693632
19	22.97409691220958	30.71833099937331	24.191302194954005	22.116269893463105
20	26.305599182985045	30.707305939605885	22.970035048084732	20.01705982932434
21	22.996727298047983	31.126064788667012	25.060734539771452	20.81647337351355
22	23.087248841401614	27.68469876441962	24.45861153878887	24.769440855389902
23	21.843351308693936	32.16609542672784	22.400987226404435	23.589566038173786
24	20.481853138467013	30.361854066893102	27.71487261220416	21.441420182435724
25	21.90602006947722	29.143875095743937	25.259572459787545	23.690532374991296
26	24.958027404043296	29.80595894809325	21.70234659693155	23.533667050931907
27	21.22942955953919	28.393010498951654	25.200191874724375	25.177368066784783
28	20.584753696296353	30.38158312121376	26.9069484955629	22.126714686926988
29	22.73541403934979	30.993570649356677	23.863064889246505	22.407950422047023
30	23.703878499972923	26.939830252764	27.10249823985888	22.253793007404198
31	27.93382643074328	27.439246118018428	22.318202567098126	22.30872488414016
32	26.209468398697112	26.686834144416675	24.681240377250464	22.422457079635745
33	23.971768110111334	26.643314171650513	23.51819328283727	25.866724435400883
34	21.75824558417343	27.24253584111535	27.92899087821371	23.070227696497515
35	21.912983265119806	27.03886236856968	28.82202071937548	22.226133646935033
36	24.633465118258272	29.540390403169027	24.300005415818834	21.526139062753867
37	22.487253483532044	28.004812341877432	23.93830608660668	25.569628087983848
38	22.780674811026607	30.38371076432678	24.67930615623864	22.156308268407983
39	22.615105492413985	26.350473110459493	25.23462100873494	25.79980038839158
40	24.50870786299526	26.74969632730114	25.356476932480216	23.385118877223388
41	20.34491029082947	24.351649116834686	30.130714655979453	25.172725936356393
42	24.47118397536576	26.039063527554912	25.099418960008045	24.390333537071278
43	22.78860511717511	25.41353645232919	25.685101082390076	26.112757348105625
44	23.706006143085933	24.754160509396446	25.542935838020597	25.996897509497025
45	25.805216207224703	26.27465164679577	25.11721379331688	22.802918352662648
46	22.900983357962414	25.22417621527106	27.67309343834864	24.201746988417884
47	22.228841556351593	28.101910236671284	25.395935041121536	24.273313165855583
48	22.332322380484484	26.117399478534015	29.27095341622115	22.27932472476035
49	24.2833711151171	24.822438511114033	27.653944650331525	23.24024572343734
50	21.99847583384268	26.028231889888666	26.10463361985594	25.86865865641272
51	20.84123140246497	24.47234450797286	28.54542711468383	26.140996974878338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3379.0
1	2735.0
2	2091.0
3	1532.0
4	973.0
5	988.0
6	1003.0
7	1112.5
8	1222.0
9	1334.0
10	1446.0
11	1546.5
12	1647.0
13	1684.0
14	1721.0
15	1625.5
16	1530.0
17	1377.0
18	1224.0
19	1258.5
20	1293.0
21	1397.5
22	1502.0
23	1558.0
24	1614.0
25	2062.5
26	2861.0
27	3211.0
28	3991.0
29	4771.0
30	5442.5
31	6114.0
32	7340.0
33	8566.0
34	10303.5
35	12041.0
36	13092.5
37	14144.0
38	15228.5
39	16313.0
40	18660.5
41	21008.0
42	25040.5
43	29073.0
44	35506.5
45	41940.0
46	44175.0
47	46410.0
48	46398.5
49	46387.0
50	45230.0
51	44073.0
52	41512.0
53	38951.0
54	35423.5
55	31896.0
56	28952.5
57	26009.0
58	23740.5
59	21472.0
60	20618.0
61	19764.0
62	18176.0
63	16588.0
64	14720.0
65	12852.0
66	11024.0
67	9196.0
68	7961.0
69	6726.0
70	5808.5
71	4891.0
72	4537.0
73	4183.0
74	3743.0
75	2485.0
76	1667.0
77	1396.0
78	1125.0
79	840.0
80	555.0
81	399.5
82	244.0
83	210.5
84	177.0
85	128.5
86	80.0
87	59.5
88	39.0
89	38.0
90	37.0
91	34.0
92	31.0
93	19.0
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	517004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.080641214997684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51667085971133	26.0773720653589
2	9.156091147829931	6.240909146820164
3	3.447339718955044	3.5246264432375343
4	1.8958869841007289	2.5845217635728384
5	1.1591941763405187	1.975304041118799
6	0.7949168155697199	1.6254764872320069
7	0.5619246633152746	1.3405526988214373
8	0.44922163935489245	1.224780921349374
9	0.419126105810524	1.2855677792370865
>10	5.202363524805159	41.16024200470213
>50	0.3478967166080973	7.295040047531991
>100	0.04239809734956778	2.524117506067778
>500	0.004065570978725677	0.7923496217682963
>1k	0.002903979270518341	2.3491394731816486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2781	0.5379068633898384	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGT	2552	0.49361320221893834	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	2404	0.4649867312438588	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	2366	0.457636691398906	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1765	0.34139000858794133	TruSeq Adapter, Index 1 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	613	0.11856774802516035	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	605	0.11702037121569658	No Hit
AACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	577	0.11160455238257345	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	568	0.10986375347192671	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	554	0.10715584405536516	RNA PCR Primer, Index 33 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	544	0.10522162304353545	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	542	0.10483477884116951	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4437103001137322	0.0
2	0.0	0.0	0.0	0.5858755444832149	0.0
3	0.0	0.0	0.0	0.8752350078529373	0.0
4	0.0	0.0	0.0	2.142343192702571	0.0
5	0.0	0.0	0.0	2.3435021779328595	0.0
6	0.0	0.0	0.0	3.3527787019055943	0.0
7	0.0	0.0	0.0	4.1212447099055325	0.0
8	0.0	0.0	0.0	4.697449149329599	0.0
9	0.0	0.0	0.0	5.8980201313722915	0.0
10	0.0	0.0	0.0	6.927992820171604	0.0
11	0.0	0.0	0.0	8.443841827142537	0.0
12	0.0	0.0	0.0	8.992773750299804	0.0
13	0.0	0.0	0.0	9.292384585032224	0.0
14	0.0	0.0	0.0	9.58058351579485	0.0
15	0.0	0.0	0.0	9.773038506471904	0.0
16	0.0	0.0	0.0	10.140733920820729	0.0
17	0.0	0.0	0.0	10.597210079612537	0.0
18	1.9342210118296956E-4	0.0	0.0	11.16993292121531	0.0
19	1.9342210118296956E-4	0.0	0.0	11.43182644621705	0.0
20	1.9342210118296956E-4	0.0	0.0	11.707452940402781	0.0
21	3.868442023659391E-4	0.0	0.0	12.033756025098452	0.0
22	3.868442023659391E-4	0.0	0.0	12.373985501079295	0.0
23	3.868442023659391E-4	0.0	0.0	12.747870422665976	0.0
24	3.868442023659391E-4	0.0	0.0	13.049415478410225	0.0
25	3.868442023659391E-4	0.0	0.0	13.314984023334443	0.0
26	3.868442023659391E-4	0.0	0.0	13.55328005199186	0.0
27	3.868442023659391E-4	0.0	0.0	13.79486425636939	0.0
28	3.868442023659391E-4	0.0	0.0	14.052889339347471	0.0
29	3.868442023659391E-4	0.0	0.0	14.330836898747398	0.0
30	3.868442023659391E-4	0.0	0.0	14.764102405397251	0.0
31	3.868442023659391E-4	0.0	0.0	15.00181816775112	0.0
32	3.868442023659391E-4	0.0	0.0	15.29098420901966	0.0
33	3.868442023659391E-4	0.0	0.0	15.601620103519508	0.0
34	3.868442023659391E-4	0.0	0.0	15.867575492646091	0.0
35	3.868442023659391E-4	0.0	0.0	16.200648350883164	0.0
36	3.868442023659391E-4	0.0	0.0	16.46157476537899	0.0
37	3.868442023659391E-4	0.0	0.0	16.731011752326868	0.0
38	3.868442023659391E-4	0.0	0.0	17.00857246752443	0.0
39	3.868442023659391E-4	0.0	0.0	17.318047829417182	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	40	6.8030204E-9	45.000004	1
CCGTAAG	20	7.02968E-4	45.000004	1
TATCTCG	20	7.02968E-4	45.000004	24
TATCTAG	20	7.02968E-4	45.000004	1
CGAATAA	20	7.02968E-4	45.000004	44
ACTAACG	20	7.02968E-4	45.000004	1
CGGCTAT	20	7.02968E-4	45.000004	39
GTCCCGT	20	7.02968E-4	45.000004	27
ATCGGGT	20	7.02968E-4	45.000004	4
TCGAATC	20	7.02968E-4	45.000004	20
ATCGAAT	20	7.02968E-4	45.000004	19
TGGTACG	20	7.02968E-4	45.000004	12
TAAGTCG	25	3.8878177E-5	45.0	36
CGACATT	25	3.8878177E-5	45.0	31
TACGGGT	25	3.8878177E-5	45.0	4
CGTTACG	45	3.8380676E-10	45.0	1
TAACCCG	25	3.8878177E-5	45.0	1
CCGCATA	25	3.8878177E-5	45.0	24
TAAACGG	30	2.1631768E-6	44.999996	2
ATGCGAG	30	2.1631768E-6	44.999996	1
>>END_MODULE