##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444324
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88312357648923	31.0	31.0	33.0	28.0	34.0
2	31.07399555279481	31.0	31.0	34.0	28.0	34.0
3	31.344563876810614	31.0	31.0	34.0	28.0	34.0
4	35.365463040483974	37.0	35.0	37.0	33.0	37.0
5	34.97222072181561	35.0	35.0	37.0	32.0	37.0
6	34.98558484349259	35.0	35.0	37.0	32.0	37.0
7	32.6749601642045	35.0	35.0	37.0	26.0	37.0
8	34.12058317804125	37.0	35.0	37.0	28.0	37.0
9	34.269161692818756	38.0	35.0	39.0	27.0	39.0
10	34.72363635545233	37.0	34.0	39.0	26.0	39.0
11	35.63050386654783	37.0	35.0	39.0	30.0	39.0
12	35.83315328454011	37.0	35.0	39.0	31.0	39.0
13	35.71687552326681	37.0	35.0	39.0	30.0	39.0
14	36.81393082525364	38.0	36.0	40.0	31.0	41.0
15	36.869082021227754	38.0	35.0	40.0	31.0	41.0
16	36.87604765891557	38.0	35.0	40.0	31.0	41.0
17	36.82869257568801	38.0	35.0	40.0	32.0	41.0
18	36.67508619835976	38.0	35.0	40.0	31.0	41.0
19	36.6412010154752	38.0	35.0	40.0	31.0	41.0
20	36.51163565326203	38.0	35.0	40.0	31.0	41.0
21	36.566550535195034	38.0	35.0	40.0	31.0	41.0
22	36.557374798570414	38.0	35.0	40.0	31.0	41.0
23	36.47306470053385	38.0	35.0	40.0	31.0	41.0
24	36.15993059119021	38.0	34.0	40.0	30.0	41.0
25	36.24052718286656	38.0	35.0	40.0	30.0	41.0
26	36.126650822372866	38.0	35.0	40.0	30.0	41.0
27	35.99893096029024	38.0	34.0	40.0	30.0	41.0
28	35.96522807680881	38.0	34.0	40.0	30.0	41.0
29	35.61769564551994	38.0	34.0	40.0	28.0	41.0
30	35.55967942312367	38.0	34.0	40.0	28.0	41.0
31	35.483905888495784	38.0	34.0	40.0	27.0	41.0
32	35.31922876099423	38.0	34.0	40.0	27.0	41.0
33	35.32049810498645	38.0	34.0	40.0	27.0	41.0
34	35.229787272350805	38.0	34.0	40.0	27.0	41.0
35	35.13783635365184	38.0	34.0	40.0	26.0	41.0
36	34.92915755169651	38.0	34.0	40.0	25.0	41.0
37	34.81398709050153	38.0	33.0	40.0	25.0	41.0
38	34.68604891925712	38.0	33.0	40.0	24.0	41.0
39	34.63000873236647	38.0	33.0	40.0	24.0	41.0
40	34.48102060658439	37.0	33.0	40.0	23.0	41.0
41	34.44910695798561	37.0	33.0	40.0	23.0	41.0
42	34.33277518207434	37.0	33.0	40.0	23.0	41.0
43	34.267401715865	37.0	33.0	40.0	23.0	41.0
44	34.063343416065756	37.0	33.0	40.0	22.0	41.0
45	33.86306163970436	37.0	33.0	40.0	22.0	41.0
46	33.810786723201986	37.0	33.0	40.0	22.0	41.0
47	33.71695654522376	37.0	33.0	40.0	21.0	41.0
48	33.620621438409806	37.0	32.0	40.0	21.0	41.0
49	33.44490506927377	36.0	32.0	40.0	20.0	41.0
50	33.206675759130725	36.0	32.0	39.0	20.0	40.0
51	31.24551228382892	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	2.0
12	7.0
13	13.0
14	25.0
15	43.0
16	97.0
17	192.0
18	458.0
19	843.0
20	1409.0
21	2264.0
22	3182.0
23	4230.0
24	5043.0
25	5519.0
26	6195.0
27	7043.0
28	8530.0
29	10232.0
30	12790.0
31	16197.0
32	20491.0
33	27407.0
34	40318.0
35	45515.0
36	51603.0
37	67599.0
38	71231.0
39	35838.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.95115276239861	32.90954348628478	20.26494179922759	25.874361952089014
2	23.12231614767602	32.48170254138872	19.65412626821869	24.741855042716576
3	19.53394369874236	35.292264203599174	18.617270280245947	26.55652181741252
4	18.93460627830142	35.114015898308445	18.3699282505559	27.581449572834238
5	17.791296441335604	37.24691891502597	16.91445881833977	28.047325825298657
6	18.803170659248654	39.72551561473159	18.149818600840828	23.321495125178924
7	75.82754926585105	14.863478002538688	5.146469693286881	4.162503038323385
8	82.32123405442874	8.681727748219767	4.239698958417732	4.757339238933751
9	71.32812992320919	16.86044418037288	7.7758572573167335	4.0355686391011965
10	33.95360142598644	40.36671437959687	11.169551948578064	14.510132245838623
11	25.702865476544147	28.977727964278323	28.853044174971416	16.46636238420612
12	26.886911352976657	26.750524392110265	28.776748498843187	17.585815756069895
13	22.491245127429533	33.11479911055896	25.88426463571628	18.509691126295227
14	17.67899100656278	36.70407180345874	27.137404236548107	18.47953295343038
15	16.265157857779457	33.83679477138305	30.957139384773274	18.94090798606422
16	18.456351671302922	31.959110919059064	29.264230606494362	20.32030680314365
17	17.77981833076764	30.28690775200079	27.405001755475734	24.528272161755833
18	20.07228959047902	30.189906464651923	27.224502840269714	22.513301104599346
19	20.721815612030863	33.92186782618089	25.08574823777244	20.27056832401581
20	22.096038026305127	33.155985272008714	24.319640622608727	20.42833607907743
21	19.772958471745845	34.39517109136576	24.904124017608773	20.927746419279625
22	19.175646600228664	31.196604279759814	24.431946057381552	25.195803062629974
23	19.172045624364202	34.029897102114674	23.027565470242436	23.770491803278688
24	17.053996633087568	32.99371629711652	27.842745383999063	22.109541685796852
25	18.063620241085335	32.204202338833824	25.842853413274998	23.889324006805847
26	20.62638975162269	34.800055815125894	23.156750479379912	21.4168039538715
27	17.51852251960281	33.253436681340645	24.829178707429715	24.39886209162683
28	18.798219317435024	31.28775398132894	27.35796400824623	22.5560626929898
29	20.919419162593062	32.10224970967132	25.27817538552948	21.700155742206135
30	20.596456639749373	33.02477471394748	23.27670798786471	23.102060658438436
31	25.032633843771663	31.560752964053258	20.817466533430558	22.589146658744518
32	23.503569467325644	32.410808329057176	22.407522438580855	21.678099765036325
33	22.235125719069867	30.33799659707781	22.57496781627821	24.85190986757411
34	19.63117004708276	31.372151853152204	24.033813163367274	24.962864936397764
35	20.945301176618862	29.34570268542775	25.461375032633843	24.247621105319542
36	23.89315004366183	29.86401814891836	25.54847363635545	20.69435817106436
37	20.986487338068617	31.8911424996174	24.260899703819735	22.861470458494253
38	20.915593125737075	32.07659275663705	26.10955068823651	20.898263429389363
39	20.894887514516434	29.264230606494362	25.780961640604605	24.059920238384603
40	22.54908580225241	27.143931005302434	26.35104113214681	23.95594206029834
41	19.39643143291832	25.254093859435905	29.51787434394721	25.831600363698566
42	22.32897615253734	27.30012333342336	25.904295063962334	24.46660545007697
43	20.42293461528074	26.818267750560405	27.710859642963243	25.047937991195614
44	21.81988818969941	28.282964683429206	25.97631458125152	23.920832545619863
45	23.098909804557035	29.03849443199107	25.62296882455145	22.239626938900443
46	20.887685562787514	28.440957499482362	28.00321387095903	22.668143066771094
47	20.701110000810218	26.85135171631512	26.59973352778603	25.847804755088628
48	21.731214159037098	25.673157425662353	30.587139114700086	22.008489300600463
49	21.46339157911794	24.796319802666524	28.924163448294486	24.81612516992105
50	20.047082759427806	26.551570475598886	27.279642783194248	26.12170398177906
51	19.592459556539822	26.309629909705528	29.53002763748976	24.567882896264887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4557.0
1	3608.5
2	2660.0
3	2042.5
4	1425.0
5	1441.0
6	1457.0
7	1646.0
8	1835.0
9	2130.5
10	2426.0
11	2661.5
12	2897.0
13	2989.5
14	3082.0
15	2962.5
16	2843.0
17	2628.0
18	2413.0
19	2224.0
20	2035.0
21	1854.0
22	1673.0
23	1692.0
24	1711.0
25	1915.5
26	2601.5
27	3083.0
28	3605.0
29	4127.0
30	5119.5
31	6112.0
32	7053.5
33	7995.0
34	8926.0
35	9857.0
36	11528.5
37	13200.0
38	14486.0
39	15772.0
40	17551.0
41	19330.0
42	23631.0
43	27932.0
44	32865.0
45	37798.0
46	40724.0
47	43650.0
48	46814.5
49	49979.0
50	46705.5
51	43432.0
52	39461.0
53	35490.0
54	30808.5
55	26127.0
56	22313.5
57	18500.0
58	16074.5
59	13649.0
60	11605.0
61	9561.0
62	8367.5
63	7174.0
64	6154.5
65	5135.0
66	4258.5
67	3382.0
68	2824.5
69	2267.0
70	2243.5
71	2220.0
72	2105.5
73	1991.0
74	1592.5
75	1037.0
76	880.0
77	673.5
78	467.0
79	444.5
80	422.0
81	338.5
82	255.0
83	177.0
84	99.0
85	73.5
86	48.0
87	37.0
88	26.0
89	22.5
90	19.0
91	11.5
92	4.0
93	5.5
94	7.0
95	5.5
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.211719965091085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06810020190724	34.95736704298485
2	9.242391930329337	8.172440876626766
3	3.3745407621792434	4.475827535647612
4	1.7705423763596122	3.131148949197523
5	1.0922236318455694	2.4144542675205534
6	0.7371471814506673	1.9554326855611852
7	0.5435843774861752	1.6822960192371998
8	0.4348740386468355	1.538122337339367
9	0.34043227996688324	1.3545986966075996
>10	3.122779090696944	28.84340811609367
>50	0.2387589180694694	6.700967087362793
>100	0.03100765169733296	2.5117202397496508
>500	0.002583970974777747	0.754224371091931
>1k	0.0010335883899110986	1.5079917749793228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3801	0.855456828800605	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	2799	0.6299457152888432	TruSeq Adapter, Index 13 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	783	0.17622275636697543	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	766	0.17239671951098748	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	697	0.15686751109550687	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	538	0.1210828134424429	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	517	0.11635653262034013	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	493	0.11095506882365119	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC	480	0.10802927593377806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.250609915287043E-4	0.0	0.0	0.8061684716558187	0.0
2	2.250609915287043E-4	0.0	0.0	1.0510348304390489	0.0
3	2.250609915287043E-4	0.0	0.0	1.3499158271891682	0.0
4	2.250609915287043E-4	0.0	0.0	2.839819591109191	0.0
5	2.250609915287043E-4	0.0	0.0	3.1002151583079014	0.0
6	2.250609915287043E-4	0.0	0.0	4.475562877539813	0.0
7	2.250609915287043E-4	0.0	0.0	5.611895823768241	0.0
8	2.250609915287043E-4	0.0	0.0	6.674183703783726	0.0
9	2.250609915287043E-4	0.0	0.0	7.98133794258244	0.0
10	2.250609915287043E-4	0.0	0.0	9.972677595628415	0.0
11	2.250609915287043E-4	0.0	0.0	11.799722724858437	0.0
12	2.250609915287043E-4	0.0	0.0	12.774236818177727	0.0
13	2.250609915287043E-4	0.0	0.0	13.277248134244381	0.0
14	2.250609915287043E-4	0.0	0.0	13.72556962936956	0.0
15	2.250609915287043E-4	0.0	0.0	14.066537031535546	0.0
16	2.250609915287043E-4	0.0	0.0	14.704810003510952	0.0
17	2.250609915287043E-4	0.0	0.0	15.506027133353138	0.0
18	2.250609915287043E-4	0.0	0.0	16.595997515326655	0.0
19	2.250609915287043E-4	0.0	0.0	17.068625597536933	0.0
20	2.250609915287043E-4	0.0	0.0	17.67336448177456	0.0
21	2.250609915287043E-4	0.0	0.0	18.216211593341797	0.0
22	2.250609915287043E-4	0.0	0.0	18.80789694007076	0.0
23	2.250609915287043E-4	0.0	0.0	19.45359692476661	0.0
24	2.250609915287043E-4	0.0	0.0	19.9419792763839	0.0
25	2.250609915287043E-4	0.0	0.0	20.363068391534107	0.0
26	2.250609915287043E-4	0.0	0.0	20.752423906878764	0.0
27	2.250609915287043E-4	0.0	0.0	21.165860948316993	0.0
28	2.250609915287043E-4	0.0	0.0	21.62183451715415	0.0
29	2.250609915287043E-4	0.0	0.0	22.071956500211556	0.0
30	2.250609915287043E-4	0.0	0.0	22.732510510348305	0.0
31	2.250609915287043E-4	0.0	0.0	23.1621519431766	0.0
32	2.250609915287043E-4	0.0	0.0	23.54092959191941	0.0
33	2.250609915287043E-4	0.0	0.0	23.957292426247513	0.0
34	2.250609915287043E-4	0.0	0.0	24.35227446638039	0.0
35	2.250609915287043E-4	0.0	0.0	24.809823462158246	0.0
36	2.250609915287043E-4	0.0	0.0	25.243741053825588	0.0
37	2.250609915287043E-4	0.0	0.0	25.626119678432854	0.0
38	2.250609915287043E-4	0.0	0.0	26.061387636049368	0.0
39	2.250609915287043E-4	0.0	0.0	26.486077727064036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTC	30	2.1624837E-6	45.000004	43
TTAGCGG	30	2.1624837E-6	45.000004	2
GCGCGAC	60	0.0	45.000004	9
ACGGGTC	30	2.1624837E-6	45.000004	5
TCTAGCG	35	1.2096825E-7	45.000004	1
GTTACGG	35	1.2096825E-7	45.000004	2
AATGCGG	70	0.0	45.000004	2
AGTGCGC	35	1.2096825E-7	45.000004	13
ACCAGTT	30	2.1624837E-6	45.000004	18
GACGGGT	35	1.2096825E-7	45.000004	4
ACGTCGC	30	2.1624837E-6	45.000004	45
ATACGAG	30	2.1624837E-6	45.000004	1
TTACCGG	60	0.0	45.000004	2
CGAAGCA	30	2.1624837E-6	45.000004	14
TAATAGC	20	7.02861E-4	45.0	17
GGTACGT	25	3.88693E-5	45.0	9
CAAACGT	20	7.02861E-4	45.0	37
GTCGATC	25	3.88693E-5	45.0	10
TACTTCG	25	3.88693E-5	45.0	21
ATCTACG	20	7.02861E-4	45.0	1
>>END_MODULE