FastQCFastQC Report
Sat 18 Jun 2016
SRR3553577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517504
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30700.5932321296067277No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT20700.39999690823645806No Hit
AATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT10850.20966021518674252No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGT9200.17777640366064804No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC9090.17565081622557507No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.14569935691318328No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7480.14453994558496167No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240.12057877813504825No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6220.1201923076923077No Hit
AAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT6180.11941936680682662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT207.029688E-445.012
ACAGTAC207.029688E-445.025
AGATCGG207.029688E-445.02
CGAGTTT207.029688E-445.022
CCGATCG207.029688E-445.026
TAGACGG453.8380676E-1045.02
CAATTCG207.029688E-445.038
TTATCCG207.029688E-445.01
GCACCGT551.8189894E-1245.024
ACCTATC207.029688E-445.038
TACGCCC207.029688E-445.030
GATATCG207.029688E-445.012
AACGATT207.029688E-445.010
GCGATTA351.2101918E-745.023
TCCGTTA207.029688E-445.031
TCCGTCC207.029688E-445.042
TCCGTCA207.029688E-445.045
GCTAGAT207.029688E-445.040
CGTAACG207.029688E-445.01
CGGTCTA207.029688E-445.031