##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517504
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979115137274302	31.0	31.0	34.0	28.0	34.0
2	31.179074944348255	31.0	31.0	34.0	28.0	34.0
3	31.41229246537225	31.0	31.0	34.0	28.0	34.0
4	35.41461322038091	37.0	35.0	37.0	33.0	37.0
5	35.06750092752906	37.0	35.0	37.0	32.0	37.0
6	35.09542728172149	36.0	35.0	37.0	32.0	37.0
7	32.78381809609201	35.0	35.0	37.0	26.0	37.0
8	34.20703994558496	37.0	35.0	37.0	29.0	37.0
9	34.42923532958199	38.0	35.0	39.0	28.0	39.0
10	34.929169629606726	37.0	34.0	39.0	27.0	39.0
11	35.83227376020282	37.0	35.0	39.0	31.0	39.0
12	35.90270220133564	37.0	35.0	39.0	31.0	39.0
13	35.78882482067772	37.0	35.0	39.0	30.0	39.0
14	36.893175704922086	39.0	35.0	40.0	31.0	41.0
15	36.963250525599804	39.0	35.0	40.0	32.0	41.0
16	36.96837319131833	38.0	35.0	40.0	32.0	41.0
17	36.92781505070492	38.0	35.0	40.0	32.0	41.0
18	36.79927111674499	38.0	35.0	40.0	31.0	41.0
19	36.759860793346526	38.0	35.0	40.0	31.0	41.0
20	36.70422837311403	38.0	35.0	40.0	31.0	41.0
21	36.76474384739055	38.0	35.0	40.0	31.0	41.0
22	36.771105150878064	38.0	35.0	40.0	31.0	41.0
23	36.6971173169676	38.0	35.0	40.0	31.0	41.0
24	36.40629057939649	38.0	35.0	40.0	30.0	41.0
25	36.49663384244373	38.0	35.0	40.0	31.0	41.0
26	36.378062778258716	38.0	35.0	40.0	30.0	41.0
27	36.26029557259461	38.0	35.0	40.0	30.0	41.0
28	36.1647542820925	38.0	35.0	40.0	30.0	41.0
29	35.819280623917884	38.0	34.0	40.0	29.0	41.0
30	35.82354725760574	38.0	34.0	40.0	29.0	41.0
31	35.71191140551571	38.0	34.0	40.0	29.0	41.0
32	35.62684539636409	38.0	34.0	40.0	29.0	41.0
33	35.56527099307445	38.0	34.0	40.0	29.0	41.0
34	35.45699743383626	38.0	34.0	40.0	28.0	41.0
35	35.34010751607717	38.0	34.0	40.0	27.0	41.0
36	35.103460069873854	38.0	34.0	40.0	26.0	41.0
37	35.087678549344545	38.0	34.0	40.0	26.0	41.0
38	34.91718711352956	38.0	34.0	40.0	26.0	41.0
39	34.8635353543161	37.0	34.0	40.0	25.0	41.0
40	34.712243770096464	37.0	33.0	40.0	25.0	41.0
41	34.622016448182045	37.0	33.0	40.0	24.0	41.0
42	34.536867347885234	37.0	33.0	40.0	24.0	41.0
43	34.41914072161761	37.0	33.0	40.0	24.0	41.0
44	34.296328144323525	37.0	33.0	40.0	23.0	41.0
45	34.088841825377195	37.0	33.0	40.0	23.0	41.0
46	34.01419699171407	36.0	33.0	40.0	23.0	41.0
47	33.89984618476379	36.0	33.0	40.0	23.0	41.0
48	33.83452495053179	36.0	33.0	40.0	23.0	41.0
49	33.649517684887456	36.0	33.0	40.0	22.0	41.0
50	33.44855885171902	36.0	32.0	39.0	22.0	40.0
51	31.57560328036112	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	8.0
11	17.0
12	13.0
13	23.0
14	28.0
15	55.0
16	122.0
17	224.0
18	445.0
19	875.0
20	1403.0
21	2225.0
22	3224.0
23	4076.0
24	4903.0
25	5746.0
26	6679.0
27	7917.0
28	9228.0
29	11648.0
30	14405.0
31	18063.0
32	23675.0
33	32014.0
34	47696.0
35	52593.0
36	58650.0
37	77951.0
38	85240.0
39	48346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.221277516695523	31.142947687360873	20.24795943606233	26.387815359881277
2	26.453515335147166	30.199186866188477	19.40893210487262	23.938365693791738
3	20.924862416522387	32.39472545139748	19.605259089784813	27.075153042295323
4	21.229014654959187	33.80283050952263	18.243144014345784	26.725010821172397
5	17.497449295077914	35.314702881523615	17.455710487261932	29.73213733613653
6	18.621885048231512	40.28142777640366	19.168933959930744	21.927753215434084
7	78.22490260944843	13.354486148899333	4.550882698491219	3.869728543161019
8	85.34909875092754	6.035895374721742	4.033398775661637	4.581607098689092
9	75.26299313628493	13.47409875092753	6.81579272817215	4.447115384615385
10	37.532656752411576	32.06023528320554	12.862702510511998	17.544405453870887
11	30.82082457333663	29.261223101657187	22.256253091763543	17.661699233242643
12	29.712040873114027	25.941248763294585	26.587813813999507	17.75889654959189
13	24.825508595102647	31.36400878060846	24.693722174128123	19.116760450160772
14	19.845257234726688	34.03799777393025	27.202301817956965	18.9144431733861
15	19.120045448924067	30.89000278258719	29.956676663368786	20.03327510511996
16	20.733366312144447	27.702974276527332	30.226433032401683	21.33722637892654
17	19.47424560969577	27.549738745980708	26.35399919614148	26.622016448182045
18	23.162912750432845	27.76017190205293	26.31399950531783	22.76291584219639
19	22.18263047242147	31.99569471926787	24.21913647044274	21.60253833786792
20	25.742216485283205	29.921121382636656	22.97141664605491	21.365245486025227
21	23.09991806826614	30.966137459807076	25.25912843185753	20.674816040069256
22	21.71828623546871	27.57466608953747	24.29005379668563	26.416993878308187
23	20.744187484541182	32.75568884491714	22.691225575068017	23.80889809547366
24	19.085456344298787	31.181015025970815	26.997665718525848	22.735862911204553
25	21.891618229037842	28.693691256492706	25.6454056393767	23.76928487509275
26	23.563489364333414	31.130387397971802	22.583207086322037	22.722916151372743
27	20.036173633440516	29.91609726688103	25.501831869898588	24.545897229779868
28	21.583021580509524	30.48961940390799	27.108775970813753	20.818583044768737
29	20.531435505812514	31.089228295819936	26.522693544397725	21.856642653969825
30	23.181463331684395	29.84054229532525	26.138155453870887	20.839838919119465
31	25.845404093494928	29.466245671531045	22.23345133564185	22.45489889933218
32	26.58626793222854	29.216006059856543	23.50841732624289	20.689308681672024
33	23.30010975760574	27.54799962898837	24.30551261439525	24.846377999010635
34	22.543980336383875	29.33465248577789	24.692949233242643	23.428417944595598
35	23.377017375711105	27.719206035122433	26.631484974029185	22.272291615137274
36	25.305698120207765	30.153196883502346	24.240972050457582	20.300132945832303
37	23.27325006183527	30.668748454118226	22.329102770220132	23.728898713826364
38	23.41643736087064	30.930002473410834	23.13025599802127	22.523304167697255
39	23.505518797922335	28.50605985654217	23.25083477615632	24.737586569379175
40	25.30260635666584	27.510125525599804	23.784357222359635	23.40291089537472
41	22.752481140242395	25.634584466979966	26.927907803611177	24.685026589166462
42	25.88192555033391	26.959791615137274	23.397693544397725	23.76058929013109
43	23.161366868661883	25.976804044026714	25.522701273806582	25.339127813504824
44	23.498175859510265	26.286366868661887	25.55980243630967	24.655654835518177
45	25.319611056146424	27.313025599802128	24.64096895869404	22.726394385357406
46	23.60812670046995	26.90587898837497	26.88346370269602	22.602530608459066
47	22.941658421963886	28.79687886470443	24.329280546623792	23.93218216670789
48	22.69856851348009	26.94935691318328	27.825871877318825	22.52620269601781
49	23.4894802745486	24.876136223101657	28.322099925797673	23.312283576552066
50	21.5084327850606	26.44114828097947	26.96462249567153	25.085796438288398
51	21.548046005441503	26.57061587929755	27.729254266633692	24.152083848627257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3713.0
1	3032.0
2	2351.0
3	1693.5
4	1036.0
5	1034.5
6	1033.0
7	1131.0
8	1229.0
9	1344.5
10	1460.0
11	1607.5
12	1755.0
13	1756.5
14	1758.0
15	1757.0
16	1756.0
17	1589.5
18	1423.0
19	1422.0
20	1421.0
21	1407.0
22	1393.0
23	1610.0
24	1827.0
25	1956.5
26	2880.5
27	3675.0
28	4315.0
29	4955.0
30	6115.0
31	7275.0
32	8005.5
33	8736.0
34	11018.5
35	13301.0
36	13993.5
37	14686.0
38	15245.5
39	15805.0
40	18677.5
41	21550.0
42	25046.0
43	28542.0
44	33601.0
45	38660.0
46	42989.0
47	47318.0
48	48680.5
49	50043.0
50	48961.0
51	47879.0
52	43831.5
53	39784.0
54	35378.0
55	30972.0
56	28165.0
57	25358.0
58	23669.5
59	21981.0
60	19394.5
61	16808.0
62	15308.0
63	13808.0
64	12640.0
65	11472.0
66	10010.5
67	8549.0
68	7134.0
69	5719.0
70	5494.0
71	5269.0
72	4785.0
73	4301.0
74	3418.0
75	2107.5
76	1680.0
77	1404.5
78	1129.0
79	850.0
80	571.0
81	436.5
82	302.0
83	227.5
84	153.0
85	181.5
86	210.0
87	167.0
88	124.0
89	86.0
90	48.0
91	38.0
92	28.0
93	29.0
94	30.0
95	16.0
96	2.0
97	1.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	517504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.09694088407057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0236249570712	25.82343302765079
2	8.763782369550519	5.801087740117469
3	3.3810930512613786	3.3571151052341888
4	1.8282969404479525	2.4204414302613193
5	1.116540750101041	1.8477041600374982
6	0.7853296306054649	1.5595205015194833
7	0.5805944463673193	1.3451130048327191
8	0.3912223240720279	1.0358609705872484
9	0.3176415341599712	0.9461666770576246
>10	3.888987878419398	31.293157599535515
>50	0.8404535714866962	18.302111826639695
>100	0.07586183623227465	3.9095248325336214
>500	0.004778698345340135	1.128078315774303
>1k	0.0017920118795025505	1.2306848082185482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3070	0.5932321296067277	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	2070	0.39999690823645806	No Hit
AATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT	1085	0.20966021518674252	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGT	920	0.17777640366064804	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	909	0.17565081622557507	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	754	0.14569935691318328	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	748	0.14453994558496167	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	624	0.12057877813504825	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	622	0.1201923076923077	No Hit
AAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT	618	0.11941936680682662	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5468556764778629	0.0
2	0.0	0.0	0.0	0.7211538461538461	0.0
3	0.0	0.0	0.0	0.9648234603017561	0.0
4	0.0	0.0	0.0	2.062013047242147	0.0
5	0.0	0.0	0.0	2.290610314123176	0.0
6	0.0	0.0	0.0	3.2629699480583727	0.0
7	0.0	0.0	0.0	4.050596710363592	0.0
8	0.0	0.0	0.0	4.738320863220381	0.0
9	0.0	0.0	0.0	5.657347576057383	0.0
10	0.0	0.0	0.0	6.8758888820183035	0.0
11	0.0	0.0	0.0	8.12766664605491	0.0
12	0.0	0.0	0.0	8.733459065050704	0.0
13	0.0	0.0	0.0	9.089011872372001	0.0
14	0.0	0.0	0.0	9.392391169923325	0.0
15	0.0	0.0	0.0	9.616930497155577	0.0
16	0.0	0.0	0.0	10.024077108582736	0.0
17	0.0	0.0	0.0	10.518372804847886	0.0
18	0.0	0.0	0.0	11.186000494682167	0.0
19	0.0	0.0	0.0	11.448413925302992	0.0
20	0.0	0.0	0.0	11.742904402671284	0.0
21	0.0	0.0	0.0	12.047443111550828	0.0
22	0.0	0.0	0.0	12.380773868414543	0.0
23	0.0	0.0	0.0	12.75004637645313	0.0
24	0.0	0.0	0.0	13.026565978233984	0.0
25	0.0	0.0	0.0	13.287047056641109	0.0
26	0.0	0.0	0.0	13.49651403660648	0.0
27	0.0	0.0	0.0	13.723758656937918	0.0
28	0.0	0.0	0.0	13.975544150383378	0.0
29	0.0	0.0	0.0	14.223464939401435	0.0
30	0.0	0.0	0.0	14.612640675241158	0.0
31	0.0	0.0	0.0	14.859595288152363	0.0
32	0.0	0.0	0.0	15.121429013109077	0.0
33	0.0	0.0	0.0	15.404132141973783	0.0
34	0.0	0.0	0.0	15.65340557754143	0.0
35	0.0	0.0	0.0	15.954272817214939	0.0
36	0.0	0.0	0.0	16.200840959683404	0.0
37	0.0	0.0	0.0	16.43407587187732	0.0
38	0.0	0.0	0.0	16.70016077170418	0.0
39	0.0	0.0	0.0	16.985955664110808	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAT	20	7.029688E-4	45.0	12
ACAGTAC	20	7.029688E-4	45.0	25
AGATCGG	20	7.029688E-4	45.0	2
CGAGTTT	20	7.029688E-4	45.0	22
CCGATCG	20	7.029688E-4	45.0	26
TAGACGG	45	3.8380676E-10	45.0	2
CAATTCG	20	7.029688E-4	45.0	38
TTATCCG	20	7.029688E-4	45.0	1
GCACCGT	55	1.8189894E-12	45.0	24
ACCTATC	20	7.029688E-4	45.0	38
TACGCCC	20	7.029688E-4	45.0	30
GATATCG	20	7.029688E-4	45.0	12
AACGATT	20	7.029688E-4	45.0	10
GCGATTA	35	1.2101918E-7	45.0	23
TCCGTTA	20	7.029688E-4	45.0	31
TCCGTCC	20	7.029688E-4	45.0	42
TCCGTCA	20	7.029688E-4	45.0	45
GCTAGAT	20	7.029688E-4	45.0	40
CGTAACG	20	7.029688E-4	45.0	1
CGGTCTA	20	7.029688E-4	45.0	31
>>END_MODULE