FastQCFastQC Report
Sat 18 Jun 2016
SRR3553576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397771
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT16180.40676670747741794No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT15990.40199008977527273TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC14680.36905656772364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14540.36553695467995406No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT12860.32330159815572274No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5560.13977891802067016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG302.161909E-645.0000041
AGACTCG302.161909E-645.0000041
CCAACCG302.161909E-645.0000041
CCACACG302.161909E-645.0000041
TTTAGCG253.88619E-545.01
TATCACG207.0277177E-445.01
TCACCGG351.209246E-745.02
ATCTACG207.0277177E-445.01
TTTACGT207.0277177E-445.013
CACGCGT253.88619E-545.039
CGCGACG406.7957444E-945.01
TCTAGCG207.0277177E-445.01
GTCGTGA207.0277177E-445.025
TTAACGG351.209246E-745.02
ATCGGCG207.0277177E-445.039
CGCCGTC207.0277177E-445.042
CGTCAGG207.0277177E-445.02
TGTAACG253.88619E-545.01
CGTCAAA207.0277177E-445.042
TGCGTAG453.8380676E-1045.01