##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397771
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.071151491687427	31.0	31.0	34.0	28.0	34.0
2	31.264569312493872	31.0	31.0	34.0	28.0	34.0
3	31.4600058827818	31.0	31.0	34.0	30.0	34.0
4	35.45505328442747	37.0	35.0	37.0	33.0	37.0
5	35.07053555940478	37.0	35.0	37.0	32.0	37.0
6	35.095733474788254	37.0	35.0	37.0	32.0	37.0
7	34.04018895294026	37.0	35.0	37.0	31.0	37.0
8	34.830005203999285	37.0	35.0	37.0	32.0	37.0
9	36.13026842077477	39.0	35.0	39.0	32.0	39.0
10	35.73512146436015	37.0	35.0	39.0	30.0	39.0
11	36.11700702162802	37.0	35.0	39.0	31.0	39.0
12	35.96509298063459	37.0	35.0	39.0	31.0	39.0
13	35.825148137999	37.0	35.0	39.0	30.0	39.0
14	36.886339124772796	39.0	35.0	40.0	31.0	41.0
15	36.94010372802442	39.0	35.0	40.0	32.0	41.0
16	36.91175073094821	38.0	35.0	40.0	31.0	41.0
17	36.84387247939141	38.0	35.0	40.0	31.0	41.0
18	36.69548056545098	38.0	35.0	40.0	31.0	41.0
19	36.757471007187554	38.0	35.0	40.0	31.0	41.0
20	36.743691722121525	38.0	35.0	40.0	31.0	41.0
21	36.809692008718585	39.0	35.0	40.0	31.0	41.0
22	36.80719559746689	39.0	35.0	40.0	31.0	41.0
23	36.75675702854155	38.0	35.0	40.0	31.0	41.0
24	36.45960866930973	38.0	35.0	40.0	30.0	41.0
25	36.55241583725309	38.0	35.0	40.0	31.0	41.0
26	36.45977710793396	38.0	35.0	40.0	31.0	41.0
27	36.33852141056035	38.0	35.0	40.0	30.0	41.0
28	36.25630576386916	38.0	35.0	40.0	30.0	41.0
29	36.0754127374796	38.0	35.0	40.0	30.0	41.0
30	36.007690354500454	38.0	35.0	40.0	30.0	41.0
31	35.931711964924546	38.0	35.0	40.0	30.0	41.0
32	35.79289088445362	38.0	34.0	40.0	29.0	41.0
33	35.82426320671944	38.0	34.0	40.0	29.0	41.0
34	35.78392341322017	38.0	34.0	40.0	29.0	41.0
35	35.734857493381874	38.0	34.0	40.0	29.0	41.0
36	35.5653152190582	38.0	34.0	40.0	28.0	41.0
37	35.491513961550744	38.0	34.0	40.0	28.0	41.0
38	35.43573312282695	38.0	34.0	40.0	28.0	41.0
39	35.41576434682267	38.0	34.0	40.0	28.0	41.0
40	35.25370627823547	38.0	34.0	40.0	27.0	41.0
41	35.26412181883546	38.0	34.0	40.0	27.0	41.0
42	35.17638792169364	38.0	34.0	40.0	27.0	41.0
43	35.07322303536457	37.0	34.0	40.0	27.0	41.0
44	34.971189453228114	37.0	34.0	40.0	27.0	41.0
45	34.76708709282477	37.0	33.0	40.0	26.0	41.0
46	34.65991738965385	37.0	33.0	40.0	26.0	41.0
47	34.50487089305153	37.0	33.0	40.0	26.0	41.0
48	34.439753023724705	37.0	33.0	40.0	25.0	41.0
49	34.251021316285	36.0	33.0	40.0	24.0	41.0
50	34.154347602012216	36.0	33.0	39.0	24.0	41.0
51	32.099514544800904	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	3.0
11	10.0
12	7.0
13	14.0
14	29.0
15	43.0
16	79.0
17	174.0
18	262.0
19	537.0
20	800.0
21	1220.0
22	1690.0
23	2312.0
24	2903.0
25	3402.0
26	4197.0
27	5171.0
28	6619.0
29	8163.0
30	10488.0
31	13431.0
32	17683.0
33	24187.0
34	36537.0
35	38179.0
36	44290.0
37	58905.0
38	74370.0
39	42058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.14070155943998	32.104401778912994	19.07278308373411	25.68211357791292
2	26.654532381697006	30.33051680489528	19.02652531230281	23.988425501104906
3	21.36606238262719	30.727227475105025	20.572892443139395	27.333817699128392
4	22.62507824853999	32.38119420470572	18.357798834002477	26.63592871275181
5	18.78694022440047	33.4094240153254	17.97944043180624	29.82419532846789
6	19.58740079090733	38.567416930847145	19.962742381923267	21.882439896322257
7	82.25763064677918	9.09342310022601	4.5591558962317515	4.0897903567630625
8	86.49423914765028	4.750723406180944	3.754672914817822	5.000364531350953
9	80.25899323982895	8.366874407636555	6.846401572764228	4.52773077977027
10	38.868846647945674	31.205894849046313	12.241968368734774	17.68329013427324
11	30.80013374529566	28.206430333030813	22.419935088279438	18.57350083339409
12	29.78447398126057	25.309537397145593	27.040935613707383	17.86505300788645
13	25.793232789720722	29.562738359508362	24.916094939047845	19.727933911723078
14	21.2021489751641	31.757468493178237	28.231821827131693	18.80856070452597
15	19.9803404471417	29.87724092505487	29.455138760744248	20.68727986705919
16	21.53952902549457	27.144261396632736	29.55997294925975	21.756236628612946
17	21.477181594434988	26.517016072061562	26.54793838665966	25.45786394684379
18	23.248301158204093	26.63743711834196	26.83830646276375	23.275955260690196
19	23.32422423957503	29.70251727752903	25.57652518660234	21.396733296293597
20	25.84728399003447	29.080299971591693	23.836076536499647	21.236339501874195
21	23.400650122809356	29.53810106820256	25.466662979453	21.594585829535085
22	22.48127691561225	27.107556860605726	24.75419273903829	25.656973484743734
23	22.958435883963386	29.915956668535415	23.380035246410625	23.745572201090578
24	20.668676198113992	29.664807137775252	27.518345982990212	22.148170681120543
25	22.623067041086454	27.395913729256282	25.227580693414048	24.753438536243216
26	24.516367457657797	28.92945941257658	23.452187313806185	23.101985815959434
27	21.240613317712956	27.434629472736827	25.598397067659533	25.726360141890687
28	21.821600870852826	29.680142594608455	26.882804427673207	21.615452106865508
29	22.227361974603475	28.895017484934797	26.190697662725537	22.686922877736183
30	24.300162656402804	26.468244291313344	27.547257090134774	21.684335962149078
31	27.56535795721659	27.948492977114974	22.980307765020576	21.50584130064786
32	26.016476817063083	26.004912374205258	26.694756530767705	21.283854277963954
33	24.81226635425911	26.161032352785902	24.425360320385348	24.601340972569645
34	22.468958269959348	27.072109329237176	27.360717598819424	23.098214801984057
35	24.232535805777697	25.679599568596	27.920336072765483	22.167528552860816
36	25.891279153080543	28.112657785509754	26.616068039148153	19.379995022261554
37	22.689436887053102	28.489759183047532	25.710773284125793	23.110030645773573
38	22.637145493261198	29.303795399865752	25.987817110850216	22.071241996022835
39	22.869188553212776	25.74848342387957	26.62562127455244	24.75670674835521
40	24.204127500496515	25.168752875398155	25.98882271457698	24.638296909528346
41	22.64443612028026	24.153847314158146	28.764037599523345	24.43767896603825
42	25.620268948716724	25.349761546216293	24.519887070701486	24.5100824343655
43	22.910921107873623	25.467417182248077	26.484585351873314	25.137076358004983
44	22.943351828061875	24.852490503329804	26.64271653790749	25.56144113070083
45	25.44378549466904	26.79179729040076	25.575770983807267	22.18864623112293
46	22.89357444358689	24.92514537258875	28.91512955947015	23.266150624354214
47	23.169864067516237	27.640526835792457	25.842507372332324	23.34710172435899
48	23.02430292806665	25.487277855851737	29.196447202033333	22.291972014048284
49	23.910742613212125	24.19382006229715	28.771831028405792	23.123606296084933
50	21.64411181307838	25.692421016112288	27.17065849446038	25.492808676348954
51	21.41734817269233	25.016403910792896	29.484301268820502	24.081946647694277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1764.0
1	1414.0
2	1064.0
3	746.5
4	429.0
5	438.0
6	447.0
7	460.0
8	473.0
9	525.0
10	577.0
11	632.0
12	687.0
13	700.5
14	714.0
15	713.0
16	712.0
17	747.5
18	783.0
19	725.0
20	667.0
21	728.0
22	789.0
23	1200.0
24	1611.0
25	1777.0
26	2451.0
27	2959.0
28	3444.5
29	3930.0
30	4799.5
31	5669.0
32	6675.5
33	7682.0
34	8493.0
35	9304.0
36	10138.0
37	10972.0
38	12073.0
39	13174.0
40	14833.0
41	16492.0
42	18830.0
43	21168.0
44	26605.0
45	32042.0
46	34043.0
47	36044.0
48	36538.5
49	37033.0
50	35761.0
51	34489.0
52	32616.5
53	30744.0
54	27774.0
55	24804.0
56	22602.0
57	20400.0
58	18640.5
59	16881.0
60	15868.0
61	14855.0
62	13629.0
63	12403.0
64	10982.5
65	9562.0
66	8070.0
67	6578.0
68	5598.5
69	4619.0
70	4260.0
71	3901.0
72	3762.5
73	3624.0
74	2986.5
75	2028.0
76	1707.0
77	1201.0
78	695.0
79	569.5
80	444.0
81	367.5
82	291.0
83	217.0
84	143.0
85	105.5
86	68.0
87	50.0
88	32.0
89	30.0
90	28.0
91	21.0
92	14.0
93	10.5
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.64043003428612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63203949988305	27.23847662748921
2	7.987159983912051	5.533573131907105
3	2.597717966466375	2.699582023985595
4	1.3337874923452806	1.8481188923677048
5	0.8554859553652825	1.4817200691072743
6	0.6612015733374698	1.3742584103853914
7	0.5611979189658038	1.360809607312533
8	0.5192749272543591	1.4390325428890915
9	0.45089174975510415	1.4057175699385678
>10	6.152442091267947	46.912960936325526
>50	0.21356604020187347	4.606201215504895
>100	0.030920335786803227	2.1115491944535476
>500	7.190775764372844E-4	0.13849491000544678
>1k	0.003595387882186422	1.8495048683281339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	1618	0.40676670747741794	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	1599	0.40199008977527273	TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	1468	0.36905656772364	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1454	0.36553695467995406	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT	1286	0.32330159815572274	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	556	0.13977891802067016	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.542027950755586E-4	0.0	0.0	0.5224111360556702	0.0
2	7.542027950755586E-4	0.0	0.0	0.6614358512812648	0.0
3	7.542027950755586E-4	0.0	0.0	0.910574174587891	0.0
4	7.542027950755586E-4	0.0	0.0	2.179897478700056	0.0
5	7.542027950755586E-4	0.0	0.0	2.3945938743648982	0.0
6	7.542027950755586E-4	0.0	0.0	3.4042200160393796	0.0
7	7.542027950755586E-4	0.0	0.0	4.1227238788146945	0.0
8	7.542027950755586E-4	0.0	0.0	4.574239952133262	0.0
9	7.542027950755586E-4	0.0	0.0	5.550932571756111	0.0
10	7.542027950755586E-4	0.0	0.0	6.5273737904472675	0.0
11	7.542027950755586E-4	0.0	0.0	7.893737854192488	0.0
12	7.542027950755586E-4	0.0	0.0	8.391511698942356	0.0
13	7.542027950755586E-4	0.0	0.0	8.623806159825628	0.0
14	7.542027950755586E-4	0.0	0.0	8.894313562326062	0.0
15	7.542027950755586E-4	0.0	0.0	9.103730538425376	0.0
16	7.542027950755586E-4	0.0	0.0	9.487871162050526	0.0
17	7.542027950755586E-4	0.0	0.0	9.939890037232479	0.0
18	7.542027950755586E-4	0.0	0.0	10.574174587891022	0.0
19	0.0010056037267674113	0.0	0.0	10.84518479225484	0.0
20	0.0010056037267674113	0.0	0.0	11.121725817115879	0.0
21	0.0010056037267674113	0.0	0.0	11.47067031030417	0.0
22	0.0010056037267674113	0.0	0.0	11.818609199765694	0.0
23	0.0010056037267674113	0.0	0.0	12.206520837366224	0.0
24	0.0010056037267674113	0.0	0.0	12.495883309743546	0.0
25	0.0010056037267674113	0.0	0.0	12.758597283361532	0.0
26	0.0010056037267674113	0.0	0.0	13.00798700759985	0.0
27	0.0010056037267674113	0.0	0.0	13.251091708545871	0.0
28	0.0010056037267674113	0.0	0.0	13.481626362907301	0.0
29	0.0010056037267674113	0.0	0.0	13.742831930935136	0.0
30	0.0010056037267674113	0.0	0.0	14.114402507975695	0.0
31	0.0010056037267674113	0.0	0.0	14.357507208921716	0.0
32	0.0010056037267674113	0.0	0.0	14.614187560179097	0.0
33	0.0010056037267674113	0.0	0.0	14.903298631624729	0.0
34	0.0010056037267674113	0.0	0.0	15.16123598754057	0.0
35	0.0010056037267674113	0.0	0.0	15.464676912092637	0.0
36	0.0010056037267674113	0.0	0.0	15.733424508071227	0.0
37	0.0010056037267674113	0.0	0.0	15.984071236968004	0.0
38	0.0010056037267674113	0.0	0.0	16.270668299096716	0.0
39	0.0010056037267674113	0.0	0.0	16.56153917706419	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.161909E-6	45.000004	1
AGACTCG	30	2.161909E-6	45.000004	1
CCAACCG	30	2.161909E-6	45.000004	1
CCACACG	30	2.161909E-6	45.000004	1
TTTAGCG	25	3.88619E-5	45.0	1
TATCACG	20	7.0277177E-4	45.0	1
TCACCGG	35	1.209246E-7	45.0	2
ATCTACG	20	7.0277177E-4	45.0	1
TTTACGT	20	7.0277177E-4	45.0	13
CACGCGT	25	3.88619E-5	45.0	39
CGCGACG	40	6.7957444E-9	45.0	1
TCTAGCG	20	7.0277177E-4	45.0	1
GTCGTGA	20	7.0277177E-4	45.0	25
TTAACGG	35	1.209246E-7	45.0	2
ATCGGCG	20	7.0277177E-4	45.0	39
CGCCGTC	20	7.0277177E-4	45.0	42
CGTCAGG	20	7.0277177E-4	45.0	2
TGTAACG	25	3.88619E-5	45.0	1
CGTCAAA	20	7.0277177E-4	45.0	42
TGCGTAG	45	3.8380676E-10	45.0	1
>>END_MODULE