FastQCFastQC Report
Sat 18 Jun 2016
SRR3553575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463276
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18100.39069582710954165No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT17270.37277994111501567TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT17140.36997383848936705No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC15840.3419128122328806No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT14650.3162261805057892No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5530.11936728861413066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT207.02892E-445.0000049
CGAGTTT207.02892E-445.00000429
TAGCGGA302.1626856E-645.0000043
TAACGCG207.02892E-445.0000041
CATAGCG302.1626856E-645.0000041
TCATCCG207.02892E-445.0000041
ATAACGC302.1626856E-645.00000411
TATACGG302.1626856E-645.0000042
GCATCGT302.1626856E-645.00000435
TAGACGA253.8871876E-545.030
CTCCCGT253.8871876E-545.041
ATAGCCG253.8871876E-545.01
TCTAGCG502.1827873E-1145.01
AGAACCG351.209828E-745.01
CGCTACG453.8380676E-1045.01
TAGTCGG253.8871876E-545.02
TGCGTAG502.1827873E-1145.01
CTAGCGG850.045.02
AGTACGG700.041.7857132
TTAGCGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)