##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463276
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.083233320957703	31.0	31.0	34.0	28.0	34.0
2	31.28046564035262	31.0	31.0	34.0	28.0	34.0
3	31.474250770598953	31.0	31.0	34.0	30.0	34.0
4	35.461573662352464	37.0	35.0	37.0	33.0	37.0
5	35.12078329116984	37.0	35.0	37.0	32.0	37.0
6	35.13117234650618	37.0	35.0	37.0	32.0	37.0
7	32.71960775002374	36.0	35.0	37.0	26.0	37.0
8	34.19352610538858	37.0	35.0	37.0	28.0	37.0
9	34.413323806974674	38.0	35.0	39.0	28.0	39.0
10	34.907502223296696	37.0	34.0	39.0	27.0	39.0
11	35.835517056786884	37.0	35.0	39.0	31.0	39.0
12	35.91290289158083	37.0	35.0	39.0	31.0	39.0
13	35.87462765176698	37.0	35.0	39.0	31.0	39.0
14	36.98213591897702	39.0	36.0	40.0	32.0	41.0
15	37.035272710004406	39.0	35.0	40.0	32.0	41.0
16	37.035818820746165	39.0	35.0	40.0	32.0	41.0
17	36.963496921921276	38.0	35.0	40.0	32.0	41.0
18	36.83458240875849	38.0	35.0	40.0	31.0	41.0
19	36.846186722385795	39.0	35.0	40.0	31.0	41.0
20	36.82174556851639	39.0	35.0	40.0	31.0	41.0
21	36.90711152746959	39.0	35.0	40.0	31.0	41.0
22	36.92351211804626	39.0	35.0	40.0	31.0	41.0
23	36.87122795050898	39.0	35.0	40.0	31.0	41.0
24	36.57446748806327	38.0	35.0	40.0	31.0	41.0
25	36.67635707440057	38.0	35.0	40.0	31.0	41.0
26	36.57976238786382	38.0	35.0	40.0	31.0	41.0
27	36.47515304052012	38.0	35.0	40.0	30.0	41.0
28	36.39855075592088	38.0	35.0	40.0	30.0	41.0
29	36.12817845085867	38.0	35.0	40.0	30.0	41.0
30	36.09280213091116	38.0	35.0	40.0	30.0	41.0
31	36.04404501852028	38.0	35.0	40.0	30.0	41.0
32	35.977270568732244	38.0	35.0	40.0	30.0	41.0
33	35.986304060646354	38.0	35.0	40.0	30.0	41.0
34	35.919995855602274	38.0	35.0	40.0	30.0	41.0
35	35.81999930926705	38.0	34.0	40.0	29.0	41.0
36	35.67252566504632	38.0	34.0	40.0	29.0	41.0
37	35.59515494003575	38.0	34.0	40.0	29.0	41.0
38	35.54448536077846	38.0	34.0	40.0	29.0	41.0
39	35.577642701111216	38.0	34.0	40.0	29.0	41.0
40	35.42985390998023	38.0	34.0	40.0	28.0	41.0
41	35.39305942893653	38.0	34.0	40.0	28.0	41.0
42	35.30042998126387	38.0	34.0	40.0	27.0	41.0
43	35.19970816532693	38.0	34.0	40.0	27.0	41.0
44	35.05526295340143	37.0	34.0	40.0	27.0	41.0
45	34.857154266571115	37.0	34.0	40.0	26.0	41.0
46	34.78746578713337	37.0	33.0	40.0	26.0	41.0
47	34.64986098999301	37.0	33.0	40.0	26.0	41.0
48	34.615717196660306	37.0	33.0	40.0	26.0	41.0
49	34.42423522910749	37.0	33.0	40.0	25.0	41.0
50	34.24506557645982	36.0	33.0	40.0	24.0	41.0
51	32.29430404337803	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	6.0
11	8.0
12	11.0
13	16.0
14	22.0
15	58.0
16	90.0
17	155.0
18	319.0
19	563.0
20	919.0
21	1384.0
22	1983.0
23	2553.0
24	3252.0
25	4021.0
26	4744.0
27	5820.0
28	7475.0
29	9343.0
30	11917.0
31	15635.0
32	20410.0
33	28240.0
34	42295.0
35	46105.0
36	54225.0
37	73412.0
38	82221.0
39	46064.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.162434488296395	31.950716203731684	19.107616194234105	25.779233113737813
2	26.698771358758062	30.168625182396667	18.864348681995182	24.268254776850085
3	21.40279228796657	30.567955171431283	20.666945837902244	27.3623067026999
4	22.602077379359173	32.30989733981471	18.372849014410416	26.715176266415703
5	18.670080038681043	33.27023199993093	18.12914979407524	29.930538167312793
6	19.55983042505979	38.620822144898504	20.024995898773085	21.794351531268617
7	79.08935494176258	12.631131334237041	4.396946960343294	3.8825667636570853
8	86.70662844610987	4.7174902218116195	3.765142161476096	4.810739170602406
9	77.04672808433848	12.08847425724622	6.5483642580232955	4.3164334003919915
10	38.90790802890717	31.198680700057853	12.262236765988309	17.63117450504667
11	30.774527495488652	28.328253568067414	22.379531855740424	18.517687080703514
12	29.691156027940146	25.287733446153048	27.160267313653204	17.860843212253602
13	25.79930754021361	29.730009756602975	24.881280273530248	19.589402429653166
14	21.150890613802574	31.91035149673197	28.07980555867345	18.85895233079201
15	19.904981048014577	30.005655376060925	29.53293501066319	20.55642856526131
16	21.53079373850577	27.09551109921515	29.59488512247559	21.77881003980349
17	21.41488011466167	26.698123796613682	26.610271199026066	25.27672488969858
18	23.352170196599868	26.693806715651146	26.95002547077768	23.003997616971308
19	23.399873941235892	29.725692675640442	25.58561203256806	21.288821350555608
20	25.70692200761533	29.150657491430593	23.853815004446595	21.288605496507483
21	23.43872766989872	29.596611954860602	25.47833257064903	21.48632780459165
22	22.549193137568103	27.134580681926106	24.67103843065473	25.64518774985106
23	22.754470337336706	30.2018667058082	23.397931254802753	23.64573170205234
24	20.552759046443157	29.752242723560034	27.715659779483502	21.9793384505133
25	22.738281283727197	27.47109714295582	25.18714545972595	24.603476113591032
26	24.48842590593944	28.923579032801182	23.387354406444537	23.20064065481484
27	21.143983284262514	27.61226569043076	25.61518403716143	25.628566988145295
28	21.818958892755074	29.759365907148222	26.92045346618431	21.5012217339124
29	22.036971481363164	29.08374273651128	26.203386318307015	22.675899463818546
30	24.088448354760445	26.63509441456065	27.71997686044604	21.556480370232865
31	27.64054257073537	27.96497120506998	22.972914634041047	21.4215715901536
32	26.022068917880485	26.11920323953755	26.634015144320017	21.224712698261943
33	24.796017924520157	26.10063979139865	24.56181628230256	24.54152600177864
34	22.363342802130912	26.994923112788054	27.50843989328176	23.133294191799273
35	24.38071473592416	25.56532175204414	27.988283442267676	22.065680069764028
36	25.772757492294012	28.149742270266536	26.719493347378236	19.358006890061215
37	22.49307108505513	28.759098248128545	25.649504830813598	23.09832583600273
38	22.41083069271881	29.393709149621394	26.171871627280495	22.0235885303793
39	22.74691544565227	25.864279608699782	26.68733109420734	24.70147385144061
40	24.248396204422416	25.227726020773794	26.000267659019677	24.523610115784113
41	22.39528920125368	24.341645153213204	28.758450685984165	24.504614959548952
42	25.486535024477845	25.482217943515312	24.5758467954308	24.455400236576036
43	22.893696198378503	25.63720115007037	26.41945622048196	25.049646431069167
44	22.943990191592054	24.963088957770314	26.701361607335585	25.391559243302048
45	25.36047626037179	26.812094734024644	25.604607188803218	22.222821816800355
46	22.697700722679354	25.010145140261958	28.962001053367754	23.330153083690934
47	23.083000198585722	27.72278296307169	25.93896510935166	23.255251728990924
48	22.96816584498226	25.575466892306096	29.20591612775106	22.250451134960585
49	23.585508422624958	24.420863588875747	28.905663146806653	23.08796484169264
50	21.74060387328504	25.7952063132992	27.15832462722006	25.305865186195703
51	21.5310095925539	25.001079270240634	29.491059325326585	23.97685181187888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2188.0
1	1713.0
2	1238.0
3	883.5
4	529.0
5	522.5
6	516.0
7	526.0
8	536.0
9	616.0
10	696.0
11	781.5
12	867.0
13	860.0
14	853.0
15	857.0
16	861.0
17	857.0
18	853.0
19	799.0
20	745.0
21	870.0
22	995.0
23	1409.0
24	1823.0
25	2179.5
26	3070.5
27	3605.0
28	4223.0
29	4841.0
30	5868.5
31	6896.0
32	7991.0
33	9086.0
34	10031.0
35	10976.0
36	11976.5
37	12977.0
38	14204.5
39	15432.0
40	17498.5
41	19565.0
42	22494.0
43	25423.0
44	31759.0
45	38095.0
46	40168.5
47	42242.0
48	42811.5
49	43381.0
50	41577.0
51	39773.0
52	37610.5
53	35448.0
54	31849.5
55	28251.0
56	25861.5
57	23472.0
58	21463.5
59	19455.0
60	18210.0
61	16965.0
62	15552.0
63	14139.0
64	12419.0
65	10699.0
66	9072.0
67	7445.0
68	6368.5
69	5292.0
70	4884.5
71	4477.0
72	4185.5
73	3894.0
74	3236.5
75	2185.5
76	1792.0
77	1269.5
78	747.0
79	618.5
80	490.0
81	396.5
82	303.0
83	226.5
84	150.0
85	107.5
86	65.0
87	48.0
88	31.0
89	31.5
90	32.0
91	21.5
92	11.0
93	7.5
94	4.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.209854155926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99742013830813	25.99860659158154
2	9.04159701871867	6.186234306940421
3	3.578925798576919	3.6730358881259217
4	1.8862849373146584	2.581181304082179
5	1.198322732101711	2.0497222948465157
6	0.8111422809582892	1.6649435478772936
7	0.6007684323068104	1.43865403154902
8	0.523951378103179	1.4339440183764958
9	0.4165005451249389	1.282358061412905
>10	5.616593341111629	43.305089899552804
>50	0.2862310889408551	5.965625526873413
>100	0.03842028039474565	2.4812778740092956
>500	6.40338006579094E-4	0.12113946008008657
>1k	0.0032016900328954707	1.818187194692077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1810	0.39069582710954165	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	1727	0.37277994111501567	TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	1714	0.36997383848936705	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	1584	0.3419128122328806	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT	1465	0.3162261805057892	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	553	0.11936728861413066	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1585404812681858E-4	0.0	0.0	0.4856716082853418	0.0
2	2.1585404812681858E-4	0.0	0.0	0.6238181990865057	0.0
3	2.1585404812681858E-4	0.0	0.0	0.8470112848496361	0.0
4	2.1585404812681858E-4	0.0	0.0	2.0115438744938223	0.0
5	2.1585404812681858E-4	0.0	0.0	2.2144466797330318	0.0
6	2.1585404812681858E-4	0.0	0.0	3.1305312599832495	0.0
7	2.1585404812681858E-4	0.0	0.0	3.7638470371873356	0.0
8	2.1585404812681858E-4	0.0	0.0	4.214981997772386	0.0
9	2.1585404812681858E-4	0.0	0.0	5.159775166423471	0.0
10	2.1585404812681858E-4	0.0	0.0	6.155509890432485	0.0
11	2.1585404812681858E-4	0.0	0.0	7.50805135599513	0.0
12	2.1585404812681858E-4	0.0	0.0	8.020273012200072	0.0
13	2.1585404812681858E-4	0.0	0.0	8.260950275861473	0.0
14	2.1585404812681858E-4	0.0	0.0	8.538322727704436	0.0
15	2.1585404812681858E-4	0.0	0.0	8.73064868458543	0.0
16	2.1585404812681858E-4	0.0	0.0	9.131489651956933	0.0
17	2.1585404812681858E-4	0.0	0.0	9.616081990001641	0.0
18	2.1585404812681858E-4	0.0	0.0	10.24162702147316	0.0
19	2.1585404812681858E-4	0.0	0.0	10.512092143776064	0.0
20	2.1585404812681858E-4	0.0	0.0	10.792486552292802	0.0
21	2.1585404812681858E-4	0.0	0.0	11.129650575466892	0.0
22	2.1585404812681858E-4	0.0	0.0	11.465303620304095	0.0
23	2.1585404812681858E-4	0.0	0.0	11.841105518092887	0.0
24	2.1585404812681858E-4	0.0	0.0	12.113297472780804	0.0
25	2.1585404812681858E-4	0.0	0.0	12.379661368169298	0.0
26	2.1585404812681858E-4	0.0	0.0	12.623576442552604	0.0
27	2.1585404812681858E-4	0.0	0.0	12.865548830502767	0.0
28	2.1585404812681858E-4	0.0	0.0	13.1306176016025	0.0
29	2.1585404812681858E-4	0.0	0.0	13.398492475327883	0.0
30	2.1585404812681858E-4	0.0	0.0	13.79156269696682	0.0
31	2.1585404812681858E-4	0.0	0.0	14.037420457783265	0.0
32	2.1585404812681858E-4	0.0	0.0	14.303136791027379	0.0
33	2.1585404812681858E-4	0.0	0.0	14.603173917923657	0.0
34	2.1585404812681858E-4	0.0	0.0	14.869753667360278	0.0
35	2.1585404812681858E-4	0.0	0.0	15.173892021170966	0.0
36	2.1585404812681858E-4	0.0	0.0	15.439608354415078	0.0
37	2.1585404812681858E-4	0.0	0.0	15.68913563404968	0.0
38	2.1585404812681858E-4	0.0	0.0	15.961759296833852	0.0
39	2.1585404812681858E-4	0.0	0.0	16.249708597035028	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.02892E-4	45.000004	9
CGAGTTT	20	7.02892E-4	45.000004	29
TAGCGGA	30	2.1626856E-6	45.000004	3
TAACGCG	20	7.02892E-4	45.000004	1
CATAGCG	30	2.1626856E-6	45.000004	1
TCATCCG	20	7.02892E-4	45.000004	1
ATAACGC	30	2.1626856E-6	45.000004	11
TATACGG	30	2.1626856E-6	45.000004	2
GCATCGT	30	2.1626856E-6	45.000004	35
TAGACGA	25	3.8871876E-5	45.0	30
CTCCCGT	25	3.8871876E-5	45.0	41
ATAGCCG	25	3.8871876E-5	45.0	1
TCTAGCG	50	2.1827873E-11	45.0	1
AGAACCG	35	1.209828E-7	45.0	1
CGCTACG	45	3.8380676E-10	45.0	1
TAGTCGG	25	3.8871876E-5	45.0	2
TGCGTAG	50	2.1827873E-11	45.0	1
CTAGCGG	85	0.0	45.0	2
AGTACGG	70	0.0	41.785713	2
TTAGCGG	55	6.002665E-11	40.909092	2
>>END_MODULE