FastQCFastQC Report
Sat 18 Jun 2016
SRR3553569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380224
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28730.7556072210065646No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT19820.5212716714357852No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.18015696010772597No Hit
AAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT6620.17410789429388993No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.14596658811647872No Hit
AAAAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT5260.13833950513381585No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5140.13518347079616225No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4260.11203921898670258No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3970.10441213600403973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT253.885864E-545.0000046
GCGACAA302.161656E-645.00000410
CGAGTTC207.0273236E-445.00000423
TAGCGAT207.0273236E-445.00000445
TTGTCGC1000.045.00000438
CGATTGA253.885864E-545.00000427
TCTAGTG207.0273236E-445.00000433
GCGTTCG207.0273236E-445.00000433
GCGTTCC207.0273236E-445.0000049
ACGGGTA800.045.0000045
TAGCCCG207.0273236E-445.0000041
CCATGCG207.0273236E-445.0000041
ATACTAG302.161656E-645.0000041
TACGCTA253.885864E-545.00000419
ACGATCC253.885864E-545.00000418
CGCTCGT207.0273236E-445.00000413
TCAGCGA207.0273236E-445.00000419
CGCTATT253.885864E-545.00000421
CATAGCG207.0273236E-445.0000041
CTACGAC207.0273236E-445.00000443