##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	405486
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.948111648737566	31.0	31.0	33.0	28.0	34.0
2	31.137474536728764	31.0	31.0	34.0	28.0	34.0
3	31.385616272818297	31.0	31.0	34.0	28.0	34.0
4	35.393007403461525	37.0	35.0	37.0	33.0	37.0
5	35.014801990697585	35.0	35.0	37.0	32.0	37.0
6	35.02867916524862	36.0	35.0	37.0	32.0	37.0
7	32.62164168429983	35.0	35.0	37.0	26.0	37.0
8	34.09601564542302	37.0	35.0	37.0	28.0	37.0
9	34.27278130441002	38.0	35.0	39.0	27.0	39.0
10	34.83010757461417	37.0	34.0	39.0	27.0	39.0
11	35.71618009006476	37.0	35.0	39.0	30.0	39.0
12	35.7897806582718	37.0	35.0	39.0	31.0	39.0
13	35.57398035937123	37.0	35.0	39.0	30.0	39.0
14	36.6428532674371	38.0	35.0	40.0	31.0	41.0
15	36.764887073783065	38.0	35.0	40.0	31.0	41.0
16	36.8265069570836	38.0	35.0	40.0	31.0	41.0
17	36.82477570125726	38.0	35.0	40.0	32.0	41.0
18	36.68567348811057	38.0	35.0	40.0	31.0	41.0
19	36.66615370197738	38.0	35.0	40.0	31.0	41.0
20	36.57363262850012	38.0	35.0	40.0	31.0	41.0
21	36.631674089857604	38.0	35.0	40.0	31.0	41.0
22	36.60287161578945	38.0	35.0	40.0	31.0	41.0
23	36.55199932920002	38.0	35.0	40.0	31.0	41.0
24	36.22362794276498	38.0	35.0	40.0	30.0	41.0
25	36.33264280394391	38.0	35.0	40.0	30.0	41.0
26	36.234123989484225	38.0	35.0	40.0	30.0	41.0
27	36.1096141420419	38.0	35.0	40.0	30.0	41.0
28	36.05230513507248	38.0	35.0	40.0	30.0	41.0
29	35.75139955510178	38.0	34.0	40.0	29.0	41.0
30	35.794493521354624	38.0	34.0	40.0	29.0	41.0
31	35.60559674070128	38.0	34.0	40.0	29.0	41.0
32	35.51834341999477	38.0	34.0	40.0	29.0	41.0
33	35.49798266771233	38.0	34.0	40.0	29.0	41.0
34	35.427006111185094	38.0	34.0	40.0	28.0	41.0
35	35.33715097438629	38.0	34.0	40.0	27.0	41.0
36	35.17828728982012	38.0	34.0	40.0	27.0	41.0
37	35.007881899744994	37.0	34.0	40.0	26.0	41.0
38	34.8924500475972	37.0	33.0	40.0	26.0	41.0
39	34.90674647213467	37.0	33.0	40.0	26.0	41.0
40	34.79340347138989	37.0	33.0	40.0	26.0	41.0
41	34.72453056332401	37.0	33.0	40.0	26.0	41.0
42	34.67411945171966	37.0	33.0	40.0	26.0	41.0
43	34.64149933659855	37.0	33.0	40.0	26.0	41.0
44	34.47624578900381	37.0	33.0	40.0	25.0	41.0
45	34.294431866944855	37.0	33.0	40.0	24.0	41.0
46	34.25309135210587	37.0	33.0	40.0	24.0	41.0
47	34.18652925131817	36.0	33.0	40.0	24.0	41.0
48	34.07605441371589	36.0	33.0	40.0	23.0	41.0
49	33.91012513379007	36.0	33.0	39.0	23.0	41.0
50	33.652212406840185	36.0	32.0	39.0	23.0	40.0
51	31.722412117804314	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	4.0
11	6.0
12	12.0
13	14.0
14	32.0
15	48.0
16	86.0
17	152.0
18	340.0
19	606.0
20	962.0
21	1495.0
22	2206.0
23	2855.0
24	3491.0
25	4061.0
26	4893.0
27	5860.0
28	7343.0
29	9054.0
30	11582.0
31	15006.0
32	19518.0
33	26764.0
34	40008.0
35	44360.0
36	47167.0
37	60979.0
38	63603.0
39	32970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.730678741066278	32.99374084432015	20.35631316494281	25.919267249670764
2	23.139886457238966	31.667677798986894	20.4897333076851	24.702702436089037
3	20.556813305514865	33.16612657403708	19.716833626808324	26.560226493639732
4	20.848561972546527	33.454422594121624	18.885238947830505	26.811776485501348
5	17.229201501408188	36.07202221531693	18.16289588296513	28.535880400309754
6	19.826085240920772	38.02991965197319	20.015487587734228	22.128507519371816
7	75.87635578047085	13.959051607207154	5.502532763153352	4.662059849168652
8	83.55208317919731	6.489989790029742	4.852695284177506	5.105231746595443
9	72.62642853267437	14.303329831362857	8.43333678598028	4.63690484998249
10	39.69557518631962	30.20178255229527	12.199434752371229	17.903207509013875
11	30.699703565597826	26.487720907750205	25.362897855906247	17.44967767074572
12	30.409434604400644	22.851837054793506	28.980038768292864	17.758689572512985
13	24.84746699022901	30.76259106356323	25.610995200820742	18.778946745387017
14	18.499035725031195	34.71414549454235	27.926734831782106	18.860083948644345
15	16.684176519041348	30.82794473791943	32.51530262450492	19.972576118534302
16	17.67730575161658	26.83249236718407	32.11553543155621	23.374666449643144
17	17.899754862066754	26.334571353881515	27.33855176257627	28.427122021475466
18	20.10969552586279	27.01893530232856	28.567447458111992	24.30392171369665
19	22.673039266460492	31.39393221960807	24.85239934301061	21.08062917092082
20	24.069881574209713	29.01851111998935	24.408980827944738	22.502626477856204
21	21.18396196169535	30.665423713765705	25.243781536230596	22.90683278830835
22	19.629530982573996	28.19801423477013	25.0252783080057	27.147176474650177
23	20.46211213210814	31.827239411471663	22.7260620588627	24.9845863975575
24	18.84010791987886	30.22693755148143	27.994554682529117	22.938399846110595
25	19.048006589623316	27.923282184834985	27.692694692295174	25.336016533246525
26	22.56551397582161	30.63114386193358	23.702667909619567	23.100674252625243
27	19.454432458827185	30.360850929501883	25.69015946296543	24.494557148705503
28	19.541241867783352	29.181525379421235	28.424902462723743	22.852330290071666
29	21.59630665423714	30.45456563235229	25.648974317239066	22.300153396171506
30	22.45922177337812	29.543806691229786	24.347573035813813	23.649398499578282
31	25.623819318052902	30.3630704882536	20.46211213210814	23.550998061585357
32	26.081295038546337	30.20523519924239	21.693720621673744	22.019749140537527
33	24.17346098262332	28.12131614901624	22.225428251530264	25.479794616830176
34	20.714895212165153	30.549513423398096	21.95291576034684	26.782675604089906
35	21.679910033885264	28.668807307773882	24.459290826317062	25.191991832023792
36	27.51562322743572	27.743991161223814	24.01537907597303	20.725006535367434
37	21.797300030087353	33.19399436725312	22.06389369793285	22.944811904726674
38	21.191113873228666	33.00903113794311	23.591936589672642	22.20791839915558
39	20.819707708774164	29.579319631257306	23.918211726175503	25.68276093379303
40	22.88315749495667	28.764741569376007	22.421489274598876	25.930611661068447
41	20.099584202660512	26.892913688758675	27.13681853380881	25.870683574772002
42	23.085383959002286	27.154821621461657	23.46295556443379	26.296838855102273
43	20.550154629259705	26.891433982924195	26.456400467587045	26.102010920229056
44	22.218522957636022	25.424059030398087	26.339503706663116	26.017914305302774
45	23.01805734353344	29.389670666804772	24.787538903932564	22.80473308572922
46	21.48927459887641	28.191108940875885	27.626601165021725	22.693015295225976
47	22.380304128872513	26.97232456854244	24.30022244911045	26.347148853474593
48	21.697913121538104	25.68350078671027	30.118919025564384	22.499667066187243
49	22.152922665640737	25.080274041520546	27.952876301524594	24.813926991314126
50	20.96200608652333	26.324706648318312	26.18857371154614	26.524713553612205
51	21.06188623035074	26.360712823624	28.148937324593206	24.42846362143206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3861.0
1	3046.5
2	2232.0
3	1575.0
4	918.0
5	851.0
6	784.0
7	836.0
8	888.0
9	965.5
10	1043.0
11	1162.0
12	1281.0
13	1330.0
14	1379.0
15	1378.5
16	1378.0
17	1238.5
18	1099.0
19	1060.5
20	1022.0
21	1039.0
22	1056.0
23	1186.5
24	1317.0
25	1314.0
26	1726.5
27	2142.0
28	2810.0
29	3478.0
30	3900.0
31	4322.0
32	4972.0
33	5622.0
34	6892.5
35	8163.0
36	9640.0
37	11117.0
38	11807.5
39	12498.0
40	14536.5
41	16575.0
42	20863.0
43	25151.0
44	29826.5
45	34502.0
46	37547.0
47	40592.0
48	42469.5
49	44347.0
50	41614.5
51	38882.0
52	34263.0
53	29644.0
54	26481.0
55	23318.0
56	21742.5
57	20167.0
58	17804.5
59	15442.0
60	14183.0
61	12924.0
62	11574.5
63	10225.0
64	8879.5
65	7534.0
66	6807.5
67	6081.0
68	4949.5
69	3818.0
70	3299.0
71	2780.0
72	2440.5
73	2101.0
74	1880.5
75	1444.0
76	1228.0
77	1002.0
78	776.0
79	515.5
80	255.0
81	220.0
82	185.0
83	205.5
84	226.0
85	152.0
86	78.0
87	58.0
88	38.0
89	33.0
90	28.0
91	18.0
92	8.0
93	6.0
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	405486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.82483380054259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99742997376362	29.50239825627068
2	9.27323648323327	7.015172575428527
3	3.567077777034971	4.0477237220987
4	1.8985746767105263	2.872530864179781
5	1.1250979449874652	2.127832138924144
6	0.7949048769358498	1.8040286914403565
7	0.5012054290170511	1.3270608436749722
8	0.38945170036201476	1.1784756671625536
9	0.288006869955867	0.9804430790545637
>10	3.638847362729827	31.987473429919998
>50	0.4765531524153718	11.456344240446645
>100	0.04308562747865005	2.8356896555153304
>500	0.005222500300442431	1.432660343199229
>1k	0.0013056250751106078	1.4321664926845104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3283	0.8096457090996977	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	2517	0.6207365975644042	No Hit
AATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	918	0.2263949926754561	No Hit
AAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	858	0.21159793433065507	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	797	0.196554258346774	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	773	0.19063543500885358	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	684	0.16868646513073202	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	647	0.15956161248477135	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	612	0.15092999511697075	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	513	0.126514848848049	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4661763908001756E-4	0.0	0.0	0.7901629156123763	0.0
2	2.4661763908001756E-4	0.0	0.0	1.0468918778946745	0.0
3	2.4661763908001756E-4	0.0	0.0	1.3704542203676575	0.0
4	2.4661763908001756E-4	0.0	0.0	2.9996103441302537	0.0
5	2.4661763908001756E-4	0.0	0.0	3.2716296000355127	0.0
6	2.4661763908001756E-4	0.0	0.0	4.656634261108891	0.0
7	2.4661763908001756E-4	0.0	0.0	5.776031724893091	0.0
8	2.4661763908001756E-4	0.0	0.0	6.643385961537513	0.0
9	2.4661763908001756E-4	0.0	0.0	7.886832097778962	0.0
10	2.4661763908001756E-4	0.0	0.0	9.701937921407891	0.0
11	2.4661763908001756E-4	0.0	0.0	11.437139629974894	0.0
12	2.4661763908001756E-4	0.0	0.0	12.34839180637556	0.0
13	2.4661763908001756E-4	0.0	0.0	12.76566885169895	0.0
14	2.4661763908001756E-4	0.0	0.0	13.145213398243095	0.0
15	2.4661763908001756E-4	0.0	0.0	13.438441771109236	0.0
16	2.4661763908001756E-4	0.0	0.0	14.03772263407368	0.0
17	2.4661763908001756E-4	0.0	0.0	14.768943933945932	0.0
18	2.4661763908001756E-4	0.0	0.0	15.799312430022244	0.0
19	2.4661763908001756E-4	0.0	0.0	16.208697710895073	0.0
20	2.4661763908001756E-4	0.0	0.0	16.628687550248344	0.0
21	2.4661763908001756E-4	0.0	0.0	17.076051947539497	0.0
22	2.4661763908001756E-4	0.0	0.0	17.55301046152025	0.0
23	2.4661763908001756E-4	0.0	0.0	18.12343706071233	0.0
24	2.4661763908001756E-4	0.0	0.0	18.515065871571398	0.0
25	2.4661763908001756E-4	0.0	0.0	18.894610418115544	0.0
26	2.4661763908001756E-4	0.0	0.0	19.23962849518849	0.0
27	2.4661763908001756E-4	0.0	0.0	19.596484218937274	0.0
28	2.4661763908001756E-4	0.0	0.0	19.93952935489758	0.0
29	2.4661763908001756E-4	0.0	0.0	20.322033313110687	0.0
30	2.4661763908001756E-4	0.0	0.0	20.845355943238484	0.0
31	2.4661763908001756E-4	0.0	0.0	21.190374020311427	0.0
32	2.4661763908001756E-4	0.0	0.0	21.549449302811933	0.0
33	2.4661763908001756E-4	0.0	0.0	21.90704487947796	0.0
34	2.4661763908001756E-4	0.0	0.0	22.23553957473254	0.0
35	2.4661763908001756E-4	0.0	0.0	22.641472208658254	0.0
36	2.4661763908001756E-4	0.0	0.0	22.997341461850716	0.0
37	2.4661763908001756E-4	0.0	0.0	23.3172045397375	0.0
38	2.4661763908001756E-4	0.0	0.0	23.663948940284005	0.0
39	2.4661763908001756E-4	0.0	0.0	24.00058201762823	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0278783E-4	45.000004	11
CTAGACG	20	7.0278783E-4	45.000004	1
TACCACG	20	7.0278783E-4	45.000004	1
CATGCGT	20	7.0278783E-4	45.000004	2
ACCGATT	20	7.0278783E-4	45.000004	42
CGCGACG	20	7.0278783E-4	45.000004	1
TAACGCG	20	7.0278783E-4	45.000004	1
CCGCTAT	20	7.0278783E-4	45.000004	23
ATATCAC	20	7.0278783E-4	45.000004	30
CGCTATA	20	7.0278783E-4	45.000004	24
CGGATCG	20	7.0278783E-4	45.000004	1
CCTCATA	20	7.0278783E-4	45.000004	39
ACGCTAG	20	7.0278783E-4	45.000004	1
CGTACAG	20	7.0278783E-4	45.000004	1
GGTTGAC	20	7.0278783E-4	45.000004	26
CGGTAGA	20	7.0278783E-4	45.000004	25
CCTATAT	20	7.0278783E-4	45.000004	29
TATCGCG	20	7.0278783E-4	45.000004	1
TAATGCG	20	7.0278783E-4	45.000004	1
ATTTACG	25	3.886324E-5	45.0	1
>>END_MODULE