FastQCFastQC Report
Sat 18 Jun 2016
SRR3553564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258549
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22590.8737221957926737No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT10220.39528290575480857Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.20731079988706202No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT5310.20537693048513048No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT4580.17714243721692985No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.1578037431976144No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.10520249546507625No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT2620.10133475666121315No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT2600.10056120890044053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA351.207045E-745.00000424
GGTACCT700.045.0000048
AATCACG207.023131E-445.0000041
TTTACGG207.023131E-445.0000042
CCCTATC207.023131E-445.00000445
GCGCGAC207.023131E-445.0000049
CGTTGAT351.207045E-745.00000425
CGACAAT207.023131E-445.00000420
CCATGCG207.023131E-445.0000041
AGACGTC207.023131E-445.00000426
AATGCCG207.023131E-445.0000041
AATGCAG406.7793735E-945.0000041
ACTACGG351.207045E-745.0000042
CGAATGG700.045.0000042
ACGTCTT207.023131E-445.00000428
AGTTACG207.023131E-445.0000041
CCAACGG351.207045E-745.0000042
TACAGAT207.023131E-445.00000415
TCGACAA207.023131E-445.00000419
ATCGCGG207.023131E-445.0000042