##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258549
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04268049770063	31.0	31.0	33.0	28.0	34.0
2	31.20618528789514	31.0	31.0	34.0	28.0	34.0
3	31.456710333437762	31.0	31.0	34.0	30.0	34.0
4	35.46699078317843	37.0	35.0	37.0	33.0	37.0
5	35.03801213696437	37.0	35.0	37.0	32.0	37.0
6	35.068153425462874	36.0	35.0	37.0	32.0	37.0
7	33.90884513187056	36.0	35.0	37.0	30.0	37.0
8	34.71604222023678	37.0	35.0	37.0	32.0	37.0
9	35.99127051351968	39.0	35.0	39.0	32.0	39.0
10	35.70940131271055	37.0	35.0	39.0	30.0	39.0
11	36.055803735462135	37.0	35.0	39.0	31.0	39.0
12	35.958665475403116	37.0	35.0	39.0	31.0	39.0
13	35.71484322120759	37.0	35.0	39.0	30.0	39.0
14	36.8047604129198	39.0	35.0	40.0	31.0	41.0
15	36.87432169530727	38.0	35.0	40.0	31.0	41.0
16	36.877087128552034	38.0	35.0	40.0	32.0	41.0
17	36.833486109016086	38.0	35.0	40.0	32.0	41.0
18	36.65744984509706	38.0	35.0	40.0	31.0	41.0
19	36.63423180905747	38.0	35.0	40.0	31.0	41.0
20	36.564299223744825	38.0	35.0	40.0	31.0	41.0
21	36.61608824632855	38.0	35.0	40.0	31.0	41.0
22	36.54868129445482	38.0	35.0	40.0	31.0	41.0
23	36.49942564078763	38.0	35.0	40.0	31.0	41.0
24	36.13802025921586	38.0	34.0	40.0	30.0	41.0
25	36.23292296624624	38.0	35.0	40.0	30.0	41.0
26	36.05349082765743	38.0	35.0	40.0	30.0	41.0
27	35.980177838630205	38.0	34.0	40.0	30.0	41.0
28	35.93458493361026	38.0	34.0	40.0	30.0	41.0
29	35.65391086409153	38.0	34.0	40.0	29.0	41.0
30	35.6339571996024	38.0	34.0	40.0	29.0	41.0
31	35.35309747862107	38.0	34.0	40.0	27.0	41.0
32	35.17452784578552	38.0	34.0	40.0	27.0	41.0
33	35.13339057586763	38.0	34.0	40.0	26.0	41.0
34	34.94078878665166	38.0	34.0	40.0	25.0	41.0
35	34.84001871985581	38.0	33.0	40.0	25.0	41.0
36	34.57784017729715	37.0	33.0	40.0	24.0	41.0
37	34.45262600126088	37.0	33.0	40.0	24.0	41.0
38	34.38961666840715	37.0	33.0	40.0	24.0	41.0
39	34.24371008977022	37.0	33.0	40.0	23.0	41.0
40	33.9866833752983	37.0	33.0	40.0	23.0	41.0
41	33.90287334315739	37.0	33.0	40.0	22.0	41.0
42	33.793516896216964	37.0	33.0	40.0	22.0	41.0
43	33.74397116213948	37.0	33.0	40.0	22.0	41.0
44	33.68438091038836	36.0	33.0	40.0	21.0	41.0
45	33.480361556223386	36.0	32.0	40.0	20.0	41.0
46	33.35004583270483	36.0	32.0	40.0	20.0	41.0
47	33.18314516784053	36.0	32.0	39.0	20.0	41.0
48	32.94991665022878	35.0	32.0	39.0	18.0	40.0
49	32.79686249028231	35.0	31.0	39.0	18.0	40.0
50	32.693868473674236	35.0	31.0	39.0	18.0	40.0
51	30.52158778413376	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	7.0
11	9.0
12	5.0
13	15.0
14	17.0
15	35.0
16	66.0
17	136.0
18	286.0
19	509.0
20	853.0
21	1381.0
22	1980.0
23	2508.0
24	3055.0
25	3427.0
26	3847.0
27	4534.0
28	5306.0
29	6213.0
30	7525.0
31	9538.0
32	12045.0
33	15897.0
34	24186.0
35	25628.0
36	27310.0
37	35984.0
38	42812.0
39	23431.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.558586573531517	32.861855973142426	19.33946756707626	25.2400898862498
2	22.06661019768013	32.51337270691435	19.66551794824192	25.754499147163596
3	20.656432629791645	34.988338767506356	18.36557093626353	25.98965766643847
4	20.082073417417977	33.807904884567336	17.78927785448793	28.320743843526756
5	17.403277521862393	36.38420570182054	18.06388730956221	28.148629466754848
6	19.49263002370924	38.50179269693559	19.24316087086007	22.7624164084951
7	78.58819798181389	11.230753164777276	5.937365837810241	4.243683015598591
8	83.47972724705956	7.32627084227748	4.385629029700366	4.808372880962603
9	75.42206699697157	11.513098097459281	8.39840803870833	4.666426866860827
10	37.89030319204483	31.389407810511738	12.684636181149415	18.03565281629401
11	28.492858220298668	27.62068312002754	26.080162754448864	17.80629590522493
12	28.949251399154512	24.020591841391767	28.874990814120338	18.15516594533338
13	23.122502891134754	31.315920773238343	27.146111568793536	18.415464766833367
14	18.05151054538985	33.84078066440017	28.713706105999247	19.39400268421073
15	16.78134512220121	30.873064680196016	31.845800989367586	20.49978920823519
16	18.154392397572607	27.825286502751894	31.243207283725717	22.777113815949782
17	18.409276384747187	27.40602361641314	27.199873138167234	26.984826860672445
18	20.106053398001926	28.398485393484407	27.447021647734086	24.04843956077958
19	22.090976952144466	32.130853339212294	24.987139768477153	20.79102994016608
20	22.5957168660486	30.070508878394424	25.046316172176258	22.287458083380713
21	20.567474637302794	32.64990388669072	24.26658002931746	22.516041446689023
22	19.265206982042088	29.086169352811265	25.080739047530642	26.567884617616006
23	19.973776730909808	31.823754878185568	23.274505026126576	24.92796336477805
24	17.723913068702643	31.076507741279215	28.2631145353492	22.936464654668942
25	21.064479073599202	29.05058615581573	25.506963863716354	24.37797090686872
26	21.24703634514154	32.109194001910666	24.195026861445992	22.448742791501804
27	18.60034268165802	30.611218763174485	25.262135997431823	25.52630255773567
28	19.99350219880951	28.36560961365157	29.066830658791947	22.57405752874697
29	20.80766121702269	28.921403679766698	27.261756959029043	23.009178144181565
30	23.351086254443064	28.555128815040863	24.934925294625003	23.15885963589107
31	25.568847684578166	28.601154906806837	23.990423478721635	21.839573929893366
32	26.273549694642025	27.944026084030494	23.26986373956194	22.512560481765544
33	24.673852925364244	26.874983078642735	24.746953188757256	23.704210807235764
34	22.921380473333876	25.83881585308781	26.761658331689542	24.478145341888773
35	23.881353244452697	27.332536579139738	26.56363010493175	22.222480071475815
36	25.701897899431053	26.10104854398973	27.491887417858894	20.705166138720323
37	21.363842057018207	29.568863155533382	26.035296984324052	23.03199780312436
38	24.02561990183679	28.82084247086626	25.735547227024664	21.417990400272288
39	23.90920096384051	26.35129124459967	26.124254976812907	23.615252814746913
40	25.110907410200774	26.132764002181403	25.481823561491247	23.274505026126576
41	21.423792008478085	24.4123937822231	30.041500837365454	24.122313371933366
42	23.670561479642156	26.82857021299638	25.198318307168083	24.30255000019339
43	21.45357359726783	25.874785823963737	27.36773300225489	25.30390757651354
44	22.7399835234327	25.194837342244604	26.545451732553598	25.519727401769103
45	24.888125655098257	27.35226204703944	25.96258349481143	21.79702880305087
46	23.463250679755095	25.638853756928086	28.323064486809074	22.574831076507742
47	23.479108408850934	26.01866570746744	25.991978309720785	24.510247573960836
48	22.989839450162254	24.2654197076763	30.526515283369882	22.218225558791563
49	21.48993034202414	24.673466151483858	29.24281277436772	24.59379073212428
50	20.920212416215108	24.63246812016291	27.78815621023481	26.659163253387174
51	21.404453314458767	24.87110760436126	29.688376284572747	24.03606279660722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2695.0
1	2170.0
2	1645.0
3	1195.0
4	745.0
5	741.0
6	737.0
7	814.0
8	891.0
9	978.0
10	1065.0
11	1148.5
12	1232.0
13	1187.0
14	1142.0
15	1099.5
16	1057.0
17	992.0
18	927.0
19	854.5
20	782.0
21	776.5
22	771.0
23	960.0
24	1149.0
25	1175.5
26	1441.0
27	1680.0
28	2039.5
29	2399.0
30	2621.0
31	2843.0
32	3353.5
33	3864.0
34	4552.0
35	5240.0
36	5991.0
37	6742.0
38	7413.5
39	8085.0
40	9076.0
41	10067.0
42	12025.0
43	13983.0
44	16564.5
45	19146.0
46	21032.5
47	22919.0
48	25231.0
49	27543.0
50	26440.5
51	25338.0
52	23061.5
53	20785.0
54	18270.5
55	15756.0
56	13927.5
57	12099.0
58	11049.0
59	9999.0
60	9023.0
61	8047.0
62	7426.0
63	6805.0
64	5787.5
65	4770.0
66	4254.5
67	3739.0
68	3474.5
69	3210.0
70	2848.0
71	2486.0
72	2073.0
73	1660.0
74	1362.0
75	941.0
76	818.0
77	628.5
78	439.0
79	450.0
80	461.0
81	357.0
82	253.0
83	176.5
84	100.0
85	89.0
86	78.0
87	61.5
88	45.0
89	33.0
90	21.0
91	15.0
92	9.0
93	11.0
94	13.0
95	7.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.629742578487836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53820996370712	31.90987253400649
2	8.956393327355231	7.277919106442583
3	2.8445778828316914	3.467234013717347
4	1.476845913523794	2.4001547717825384
5	1.0091938839139398	2.0501643857603855
6	0.7206768259611741	1.756854835265051
7	0.6188597476299147	1.7600878566873968
8	0.5030726289646065	1.6351769130492066
9	0.43120505579911744	1.5767775374094561
>10	4.759226074345471	39.64492329791251
>50	0.10780126172210476	2.6725729827167592
>100	0.02994479492280688	2.08493136633488
>500	0.0019963196615204585	0.43272160433365425
>1k	0.0019963196615204585	1.330608794581743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2259	0.8737221957926737	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	1022	0.39528290575480857	Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	536	0.20731079988706202	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	531	0.20537693048513048	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT	458	0.17714243721692985	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	408	0.1578037431976144	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	272	0.10520249546507625	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	262	0.10133475666121315	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	260	0.10056120890044053	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5770666295363741	0.0
2	0.0	0.0	0.0	0.8331109383521113	0.0
3	0.0	0.0	0.0	1.1258987658045476	0.0
4	0.0	0.0	0.0	2.746868098503572	0.0
5	0.0	0.0	0.0	3.088002661004297	0.0
6	0.0	0.0	0.0	4.796382890670627	0.0
7	0.0	0.0	0.0	6.058812836251542	0.0
8	0.0	0.0	0.0	7.031162371542725	0.0
9	0.0	0.0	0.0	8.24756622535767	0.0
10	0.0	0.0	0.0	10.247573960835277	0.0
11	0.0	0.0	0.0	12.022866071808439	0.0
12	0.0	0.0	0.0	12.965434018309875	0.0
13	0.0	0.0	0.0	13.435364282979242	0.0
14	0.0	0.0	0.0	13.843023952906412	0.0
15	0.0	0.0	0.0	14.146641449009666	0.0
16	0.0	0.0	0.0	14.731443556153765	0.0
17	0.0	0.0	0.0	15.443494269944962	0.0
18	0.0	0.0	0.0	16.382194477642535	0.0
19	0.0	0.0	0.0	16.76510061922498	0.0
20	0.0	0.0	0.0	17.200994782420352	0.0
21	0.0	0.0	0.0	17.67092504708972	0.0
22	0.0	0.0	0.0	18.147817241606038	0.0
23	0.0	0.0	0.0	18.70129066443885	0.0
24	0.0	0.0	0.0	19.09734711795443	0.0
25	0.0	0.0	0.0	19.46323520879988	0.0
26	0.0	0.0	0.0	19.78580462504206	0.0
27	0.0	0.0	0.0	20.12113757933699	0.0
28	0.0	0.0	0.0	20.45376311646922	0.0
29	0.0	0.0	0.0	20.82042475507544	0.0
30	0.0	0.0	0.0	21.344890136879275	0.0
31	0.0	0.0	0.0	21.672874387446868	0.0
32	0.0	0.0	0.0	21.99737767309098	0.0
33	0.0	0.0	0.0	22.33696514007016	0.0
34	0.0	0.0	0.0	22.657213913030024	0.0
35	0.0	0.0	0.0	23.063713261316035	0.0
36	0.0	0.0	0.0	23.380481069352424	0.0
37	0.0	0.0	0.0	23.684485339336064	0.0
38	0.0	0.0	0.0	24.000092825731294	0.0
39	0.0	0.0	0.0	24.330784493461586	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	35	1.207045E-7	45.000004	24
GGTACCT	70	0.0	45.000004	8
AATCACG	20	7.023131E-4	45.000004	1
TTTACGG	20	7.023131E-4	45.000004	2
CCCTATC	20	7.023131E-4	45.000004	45
GCGCGAC	20	7.023131E-4	45.000004	9
CGTTGAT	35	1.207045E-7	45.000004	25
CGACAAT	20	7.023131E-4	45.000004	20
CCATGCG	20	7.023131E-4	45.000004	1
AGACGTC	20	7.023131E-4	45.000004	26
AATGCCG	20	7.023131E-4	45.000004	1
AATGCAG	40	6.7793735E-9	45.000004	1
ACTACGG	35	1.207045E-7	45.000004	2
CGAATGG	70	0.0	45.000004	2
ACGTCTT	20	7.023131E-4	45.000004	28
AGTTACG	20	7.023131E-4	45.000004	1
CCAACGG	35	1.207045E-7	45.000004	2
TACAGAT	20	7.023131E-4	45.000004	15
TCGACAA	20	7.023131E-4	45.000004	19
ATCGCGG	20	7.023131E-4	45.000004	2
>>END_MODULE