Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553563_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 301900 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2745 | 0.9092414706856575 | No Hit |
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT | 1073 | 0.35541570056310035 | Illumina Paired End PCR Primer 2 (95% over 21bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 621 | 0.20569725074527986 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT | 580 | 0.19211659489897318 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 507 | 0.16793640278237829 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT | 427 | 0.14143756210665784 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 378 | 0.12520702219277907 | No Hit |
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 326 | 0.10798277575356079 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT | 307 | 0.10168930109307718 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTACGG | 40 | 6.7866495E-9 | 45.000004 | 2 |
TTGTCCG | 25 | 3.88395E-5 | 45.000004 | 25 |
ATAGATC | 20 | 7.0250145E-4 | 45.000004 | 16 |
CGATTAC | 20 | 7.0250145E-4 | 45.000004 | 38 |
CGACAGT | 20 | 7.0250145E-4 | 45.000004 | 32 |
TATGCGG | 50 | 2.1827873E-11 | 45.000004 | 2 |
TCTAGCG | 20 | 7.0250145E-4 | 45.000004 | 1 |
GCTTCTA | 25 | 3.88395E-5 | 45.000004 | 22 |
CTACGCG | 20 | 7.0250145E-4 | 45.000004 | 1 |
CGCTAAG | 25 | 3.88395E-5 | 45.000004 | 1 |
GCGATGT | 25 | 3.88395E-5 | 45.000004 | 9 |
ACTCGCG | 20 | 7.0250145E-4 | 45.000004 | 1 |
CGTACAG | 25 | 3.88395E-5 | 45.000004 | 1 |
CGGCTCG | 25 | 3.88395E-5 | 45.000004 | 1 |
AACTCGC | 20 | 7.0250145E-4 | 45.000004 | 41 |
TCATACG | 20 | 7.0250145E-4 | 45.000004 | 1 |
CGCACGG | 25 | 3.88395E-5 | 45.000004 | 2 |
CGATCGT | 25 | 3.88395E-5 | 45.000004 | 27 |
TAGTACG | 20 | 7.0250145E-4 | 45.000004 | 1 |
TTTACGG | 35 | 1.2079545E-7 | 45.0 | 2 |