FastQCFastQC Report
Sat 18 Jun 2016
SRR3553563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301900
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27450.9092414706856575No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT10730.35541570056310035Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.20569725074527986No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT5800.19211659489897318No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.16793640278237829No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT4270.14143756210665784No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.12520702219277907No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA3260.10798277575356079No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT3070.10168930109307718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG406.7866495E-945.0000042
TTGTCCG253.88395E-545.00000425
ATAGATC207.0250145E-445.00000416
CGATTAC207.0250145E-445.00000438
CGACAGT207.0250145E-445.00000432
TATGCGG502.1827873E-1145.0000042
TCTAGCG207.0250145E-445.0000041
GCTTCTA253.88395E-545.00000422
CTACGCG207.0250145E-445.0000041
CGCTAAG253.88395E-545.0000041
GCGATGT253.88395E-545.0000049
ACTCGCG207.0250145E-445.0000041
CGTACAG253.88395E-545.0000041
CGGCTCG253.88395E-545.0000041
AACTCGC207.0250145E-445.00000441
TCATACG207.0250145E-445.0000041
CGCACGG253.88395E-545.0000042
CGATCGT253.88395E-545.00000427
TAGTACG207.0250145E-445.0000041
TTTACGG351.2079545E-745.02