##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553563_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 301900 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.044193441536933 31.0 31.0 34.0 28.0 34.0 2 31.223265982113283 31.0 31.0 34.0 28.0 34.0 3 31.48305730374296 31.0 31.0 34.0 30.0 34.0 4 35.466465717124876 37.0 35.0 37.0 33.0 37.0 5 35.07329248095396 37.0 35.0 37.0 32.0 37.0 6 35.081288506127855 36.0 35.0 37.0 32.0 37.0 7 32.48419344153693 35.0 35.0 37.0 25.0 37.0 8 34.05309705200398 37.0 35.0 37.0 27.0 37.0 9 34.24218946671083 38.0 34.0 39.0 27.0 39.0 10 34.85325604504803 37.0 34.0 39.0 27.0 39.0 11 35.753216296787016 37.0 35.0 39.0 30.0 39.0 12 35.917085127525674 37.0 35.0 39.0 32.0 39.0 13 35.779701888042396 37.0 35.0 39.0 30.0 39.0 14 36.90413050679033 39.0 36.0 40.0 31.0 41.0 15 36.95936402782378 38.0 35.0 40.0 32.0 41.0 16 36.9927923153362 38.0 35.0 40.0 32.0 41.0 17 36.934402119907254 38.0 35.0 40.0 32.0 41.0 18 36.78183835707188 38.0 35.0 40.0 31.0 41.0 19 36.74411725736999 38.0 35.0 40.0 31.0 41.0 20 36.647505796621395 38.0 35.0 40.0 31.0 41.0 21 36.70184166942696 38.0 35.0 40.0 31.0 41.0 22 36.66359721762173 38.0 35.0 40.0 31.0 41.0 23 36.59122557138125 38.0 35.0 40.0 31.0 41.0 24 36.25578005962239 38.0 35.0 40.0 30.0 41.0 25 36.35810864524677 38.0 35.0 40.0 30.0 41.0 26 36.17643590592912 38.0 35.0 40.0 30.0 41.0 27 36.108714806227226 38.0 35.0 40.0 30.0 41.0 28 36.05190129181848 38.0 35.0 40.0 30.0 41.0 29 35.684610798277575 38.0 34.0 40.0 29.0 41.0 30 35.6920470354422 38.0 34.0 40.0 29.0 41.0 31 35.43591917853594 38.0 34.0 40.0 27.0 41.0 32 35.32631666114607 38.0 34.0 40.0 27.0 41.0 33 35.29189135475323 38.0 34.0 40.0 27.0 41.0 34 35.071421000331235 38.0 34.0 40.0 26.0 41.0 35 34.92073865518383 38.0 34.0 40.0 25.0 41.0 36 34.65379927128188 37.0 33.0 40.0 24.0 41.0 37 34.50113282543889 37.0 33.0 40.0 24.0 41.0 38 34.41092083471348 37.0 33.0 40.0 24.0 41.0 39 34.344448492878435 37.0 33.0 40.0 23.0 41.0 40 34.08675057966214 37.0 33.0 40.0 23.0 41.0 41 33.953564094070884 37.0 33.0 40.0 22.0 41.0 42 33.84670420669096 37.0 33.0 40.0 22.0 41.0 43 33.8002550513415 37.0 33.0 40.0 22.0 41.0 44 33.68010268300762 37.0 33.0 40.0 21.0 41.0 45 33.46828088771116 36.0 32.0 40.0 20.0 41.0 46 33.35918847300431 36.0 32.0 40.0 20.0 41.0 47 33.22241801921166 36.0 32.0 40.0 20.0 41.0 48 33.03413713150049 36.0 32.0 39.0 18.0 41.0 49 32.89359390526664 36.0 31.0 39.0 18.0 40.0 50 32.685319642265654 35.0 31.0 39.0 17.0 40.0 51 30.580506790327924 34.0 27.0 38.0 10.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 6.0 10 3.0 11 5.0 12 10.0 13 6.0 14 19.0 15 26.0 16 79.0 17 180.0 18 315.0 19 571.0 20 1046.0 21 1525.0 22 2231.0 23 2867.0 24 3494.0 25 4057.0 26 4548.0 27 5211.0 28 6153.0 29 7466.0 30 8948.0 31 11184.0 32 14278.0 33 19013.0 34 27971.0 35 30642.0 36 33235.0 37 44275.0 38 47238.0 39 25296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.460417356740642 32.794302749254726 19.295462073534285 25.449817820470354 2 22.030473666777077 32.29910566412719 19.64392182842001 26.02649884067572 3 20.592249089102353 35.04637297118251 18.380920834713482 25.98045710500166 4 20.21066578337198 33.88638622060285 17.793640278237827 28.109307717787345 5 17.442199403776083 36.33984763166612 18.020205366015237 28.197747598542566 6 19.385226896323285 38.54521364690295 19.265319642265652 22.804239814508115 7 75.30407419675389 14.982113282543889 5.641603179860881 4.072209340841338 8 83.44120569725074 7.472341835044717 4.413713150049685 4.672739317654853 9 72.13116926134482 15.40675720437231 7.987744286187478 4.474329248095396 10 37.85028155018218 31.443193110301426 12.794965220271612 17.911560119244783 11 28.304736667770786 27.88340510102683 26.226233852268965 17.58562437893342 12 28.916859887379925 24.094733355415702 28.788340510102685 18.20006624710169 13 23.057634978469693 31.320967207684664 27.144749917191124 18.476647896654523 14 17.90824776416032 33.89367340178867 28.817820470354423 19.380258363696587 15 16.87512421331567 31.131500496853263 31.812851937727725 20.180523352103346 16 18.229214971844982 28.042398145081155 31.166280225240143 22.56210665783372 17 18.40841338191454 27.710168930109308 27.024180192116592 26.857237495859554 18 20.030804902285524 28.605829744948657 27.386220602848628 23.977144749917194 19 22.029811195760185 32.303411725737 24.903941702550515 20.762835375952303 20 22.54223252732693 29.929115601192446 25.22888373633653 22.299768135144088 21 20.494865849619078 32.97118251076515 24.204703544219942 22.329248095395826 22 19.40476979132163 29.159655515071215 25.180192116594903 26.255382577012256 23 20.06061609804571 31.919841006955945 23.281881417687977 24.73766147731037 24 17.531964226565087 31.12984431931103 28.303080490228556 23.03511096389533 25 21.203378602186156 29.041073203047368 25.360052997681354 24.395495197085125 26 21.23683338853925 32.299436899635644 23.99602517389864 22.467704537926465 27 18.647234183504473 30.795958926796953 25.18880423981451 25.36800264988407 28 19.818482941371315 28.570387545544882 29.100364359059288 22.51076515402451 29 20.741636303411724 28.95462073534283 27.29181848294137 23.011924478304074 30 23.209672076846637 28.6588274263001 25.072540576349784 23.058959920503476 31 25.51076515402451 28.710500165617752 24.024842663133487 21.753892017224246 32 26.101689301093078 28.18052335210335 23.25306392845313 22.464723418350445 33 24.345478635309707 27.06425968863862 24.895329579330905 23.69493209672077 34 22.83603842331898 25.95428949983438 26.6823451473998 24.527326929446836 35 23.83206359721762 27.464392182842 26.48426631334879 22.219277906591586 36 25.684995031467373 26.086783703212983 27.47466048360384 20.753560781715798 37 21.29943689963564 29.758529314342496 26.12487578668433 22.817157999337528 38 23.98211328254389 28.946671083140114 25.748261013580652 21.32295462073534 39 23.72341835044717 26.46273600529977 26.29943689963564 23.514408744617423 40 24.915534945346142 26.42000662471017 25.55846306724081 23.105995362702885 41 21.551507121563432 24.697581980788343 30.00761841669427 23.743292480953958 42 23.413713150049688 26.77542232527327 25.402451142762505 24.40841338191454 43 21.296455780059624 26.09672076846638 27.2792315336204 25.327591917853592 44 22.716793640278237 25.421994037760847 26.535607817157995 25.32560450480291 45 24.818482941371315 27.59622391520371 25.82543888704869 21.759854256376286 46 23.456442530639286 25.702881748923485 28.27691288506128 22.563762835375954 47 23.423981450811524 26.2749254720106 26.074527989400465 24.22656508777741 48 22.964889036104672 24.519046041735674 30.46737330241802 22.048691619741636 49 21.39615766810202 24.878436568400133 29.219277906591586 24.50612785690626 50 21.007287181185823 24.71745611129513 27.723418350447165 26.551838357071876 51 21.31169261344816 25.029479960251738 29.76117919841007 23.89764822789003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3299.0 1 2611.5 2 1924.0 3 1403.5 4 883.0 5 889.5 6 896.0 7 970.0 8 1044.0 9 1211.5 10 1379.0 11 1438.0 12 1497.0 13 1445.5 14 1394.0 15 1348.0 16 1302.0 17 1178.5 18 1055.0 19 962.0 20 869.0 21 902.5 22 936.0 23 1081.5 24 1227.0 25 1346.0 26 1712.0 27 1959.0 28 2418.5 29 2878.0 30 3188.0 31 3498.0 32 4111.0 33 4724.0 34 5647.5 35 6571.0 36 7374.0 37 8177.0 38 8841.5 39 9506.0 40 10835.5 41 12165.0 42 14342.0 43 16519.0 44 19595.0 45 22671.0 46 24925.0 47 27179.0 48 29472.0 49 31765.0 50 30708.0 51 29651.0 52 26740.5 53 23830.0 54 20923.5 55 18017.0 56 15833.0 57 13649.0 58 12546.0 59 11443.0 60 10438.5 61 9434.0 62 8616.5 63 7799.0 64 6558.0 65 5317.0 66 4753.0 67 4189.0 68 3890.5 69 3592.0 70 3144.0 71 2696.0 72 2257.5 73 1819.0 74 1506.5 75 1032.5 76 871.0 77 712.0 78 553.0 79 530.0 80 507.0 81 400.5 82 294.0 83 195.0 84 96.0 85 90.0 86 84.0 87 59.0 88 34.0 89 25.0 90 16.0 91 16.0 92 16.0 93 15.5 94 15.0 95 8.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 301900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.764658877333304 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23832922395594 35.918414365975515 2 8.499533284324787 7.6095741617867905 3 3.011306690399549 4.04400350312302 4 1.5647880184683751 2.801888074483006 5 0.9591999968227951 2.1469130326455805 6 0.6527295143535178 1.7531528429521592 7 0.48552360384261534 1.5214008952035734 8 0.3958206021257375 1.4175019384623446 9 0.34365592338434026 1.3845276163327485 >10 3.7015223647842994 34.51933686203609 >50 0.12335945585262226 3.3898732538695673 >100 0.02055990930877038 1.6770225289675682 >500 0.0022028474259396832 0.5614179693211695 >1k 0.0014685649506264555 1.2549729548408812 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2745 0.9092414706856575 No Hit CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT 1073 0.35541570056310035 Illumina Paired End PCR Primer 2 (95% over 21bp) CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 621 0.20569725074527986 No Hit AAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT 580 0.19211659489897318 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 507 0.16793640278237829 No Hit AAAAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT 427 0.14143756210665784 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 378 0.12520702219277907 No Hit ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA 326 0.10798277575356079 No Hit AATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT 307 0.10168930109307718 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.312355084465055E-4 0.0 0.0 0.5342828751242134 0.0 2 3.312355084465055E-4 0.0 0.0 0.7780722093408413 0.0 3 3.312355084465055E-4 0.0 0.0 1.0675720437230871 0.0 4 3.312355084465055E-4 0.0 0.0 2.5710500165617756 0.0 5 3.312355084465055E-4 0.0 0.0 2.868168267638291 0.0 6 3.312355084465055E-4 0.0 0.0 4.405101026830076 0.0 7 3.312355084465055E-4 0.0 0.0 5.564425306392845 0.0 8 3.312355084465055E-4 0.0 0.0 6.498840675720437 0.0 9 3.312355084465055E-4 0.0 0.0 7.665783371977476 0.0 10 3.312355084465055E-4 0.0 0.0 9.656177542232527 0.0 11 3.312355084465055E-4 0.0 0.0 11.388539251407751 0.0 12 3.312355084465055E-4 0.0 0.0 12.331566743954951 0.0 13 3.312355084465055E-4 0.0 0.0 12.792646571712488 0.0 14 3.312355084465055E-4 0.0 0.0 13.171579993375289 0.0 15 3.312355084465055E-4 0.0 0.0 13.493540907585293 0.0 16 3.312355084465055E-4 0.0 0.0 14.100033123550844 0.0 17 3.312355084465055E-4 0.0 0.0 14.822457767472674 0.0 18 6.62471016893011E-4 0.0 0.0 15.751904604173568 0.0 19 6.62471016893011E-4 0.0 0.0 16.137793971513748 0.0 20 6.62471016893011E-4 0.0 0.0 16.551507121563432 0.0 21 6.62471016893011E-4 0.0 0.0 17.023186485591257 0.0 22 6.62471016893011E-4 0.0 0.0 17.490228552500827 0.0 23 6.62471016893011E-4 0.0 0.0 18.009937065253396 0.0 24 6.62471016893011E-4 0.0 0.0 18.397813845644254 0.0 25 6.62471016893011E-4 0.0 0.0 18.781053328916858 0.0 26 6.62471016893011E-4 0.0 0.0 19.100033123550844 0.0 27 6.62471016893011E-4 0.0 0.0 19.433587280556477 0.0 28 6.62471016893011E-4 0.0 0.0 19.78834051010268 0.0 29 6.62471016893011E-4 0.0 0.0 20.152037098376947 0.0 30 6.62471016893011E-4 0.0 0.0 20.680357734349123 0.0 31 6.62471016893011E-4 0.0 0.0 21.01788671745611 0.0 32 6.62471016893011E-4 0.0 0.0 21.354090758529313 0.0 33 6.62471016893011E-4 0.0 0.0 21.69029479960252 0.0 34 9.937065253395165E-4 0.0 0.0 22.029811195760185 0.0 35 9.937065253395165E-4 0.0 0.0 22.42298774428619 0.0 36 9.937065253395165E-4 0.0 0.0 22.749254720105995 0.0 37 9.937065253395165E-4 0.0 0.0 23.066909572706194 0.0 38 9.937065253395165E-4 0.0 0.0 23.3839019542895 0.0 39 9.937065253395165E-4 0.0 0.0 23.730705531632992 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGG 40 6.7866495E-9 45.000004 2 TTGTCCG 25 3.88395E-5 45.000004 25 ATAGATC 20 7.0250145E-4 45.000004 16 CGATTAC 20 7.0250145E-4 45.000004 38 CGACAGT 20 7.0250145E-4 45.000004 32 TATGCGG 50 2.1827873E-11 45.000004 2 TCTAGCG 20 7.0250145E-4 45.000004 1 GCTTCTA 25 3.88395E-5 45.000004 22 CTACGCG 20 7.0250145E-4 45.000004 1 CGCTAAG 25 3.88395E-5 45.000004 1 GCGATGT 25 3.88395E-5 45.000004 9 ACTCGCG 20 7.0250145E-4 45.000004 1 CGTACAG 25 3.88395E-5 45.000004 1 CGGCTCG 25 3.88395E-5 45.000004 1 AACTCGC 20 7.0250145E-4 45.000004 41 TCATACG 20 7.0250145E-4 45.000004 1 CGCACGG 25 3.88395E-5 45.000004 2 CGATCGT 25 3.88395E-5 45.000004 27 TAGTACG 20 7.0250145E-4 45.000004 1 TTTACGG 35 1.2079545E-7 45.0 2 >>END_MODULE